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Aoki T Takano T Unajak S Takagi M Kim YR Park SB Kondo H Hirono I Saito-Taki T Hikima J Jung TS 《Comparative immunology, microbiology and infectious diseases》2011,34(3):209-216
Outbreaks of koi herpesvirus (KHV) infection in carp are still a serious problem worldwide. KHV is closely related to other two cyprinid herpesviruses, pox herpesvirus (CHV) and haematopoietic necrosis herpesvirus (CyHV-2) in goldfish. In this study, two major KHV antigenic proteins (ORF62 and ORF68) were identified by immunoscreening using a KHV-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. After screening hybridoma cells, one mAb against ORF68 (mAb-7C6) was obtained but no mAbs against ORF62. mAb-7C6 specifically reacted with a lysate of KHV-infected koi fin cells (KF-1 cells) but not with lysates of CHV- or CyHV-2-infected KF-1 cells in an immuno-blotting analysis. Similar results were shown in the following tests: (1) a indirect fluorescent antibody test using infected KF-1 cells and (2) an immunohistochemical investigation by fast red stain (infected liver) or FITC detection (infected spleen). These results suggested that mAb-7C6 specifically reacts with KHV ORF68 protein. 相似文献
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You-Jung Ha Yong Seok Choi Ye Rim Oh Eun Ha Kang Gilson Khang Yong-Beom Park Yun Jong Lee 《Marine drugs》2021,19(3)
Fucoxanthin (FX), a natural carotenoid present in edible brown seaweed, is known for its therapeutic potential in various diseases, including bone disease. However, its underlying regulatory mechanisms in osteoclastogenesis remain unclear. In this study, we investigated the effect of FX on osteoclast differentiation and its regulatory signaling pathway. In vitro studies were performed using osteoclast-like RAW264.7 cells stimulated with the soluble receptor activator of nuclear factor-κB ligand or tumor necrosis factor-alpha/interleukin-6. FX treatment significantly inhibited osteoclast differentiation and bone resorption ability, and downregulated the expression of osteoclast-specific markers such as nuclear factor of activated T cells 1, dendritic cell-specific seven transmembrane protein, and matrix metallopeptidase 9. Intracellular signaling pathway analysis revealed that FX specifically decreased the activation of the extracellular signal-regulated kinase and p38 kinase, and increased the nuclear translocation of phosphonuclear factor erythroid 2-related factor 2 (Nrf2). Our results suggest that FX regulates the expression of mitogen-activated protein kinases and Nrf2. Therefore, FX is a potential therapeutic agent for osteoclast-related skeletal disorders including osteoporosis and rheumatoid arthritis. 相似文献
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Bomi Yim Jeong-Hwan Mun Soo Hyun Park Hye Rim Shim Jun-Hyuk Kim Youn Young Hur Hee-Ju Yu 《Plant Breeding》2020,139(3):672-683
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Rim Ben Mansour Rihab Ben Fattoum Houda Gouia Chiraz Chaffei Haouari 《Journal of plant nutrition》2019,42(10):1142-1151
Two varieties of durum wheat (Om Rabiaa and Karim), were analyzed and evaluated in the presence of increasing doses of NaCl (0, 100, 200 and 300?mM) in which we added different concentrations of nitrate (0.1, 3, 10?mM). The data obtained showed that presence of NaCl in the culture medium induces the increase of the salt accumulation levels (Na+, Cl?) and reduces the levels of K+ and NO3? in the cultivar Om Rabiaa. In Karim variety, ions that have been heavily accumulated following exposure to NaCl are Na+ and K+ while low levels of NO3? and Cl? have been detected. Those findings highlight the difference in the salinity tolerance of durum wheat cultivars also depending on nitrogen (N) availability, Karim cultivar being less sensitive to NaCl treatment than Om Rabiaa. These data also suggested a relationship between salt tolerance capacity and enhancement of nitrogen and carbon metabolisms enzyme activity. 相似文献
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This study aimed to develop UV protection fabrics by microencapsulation methods. The sol-gel method was used to manufacture
spherical silica microcapsules in an O/W/O emulsion. UV protection fabrics were prepared by attaching microcapsules to cotton-knitted
fabrics. The appropriate conditions for microencapsulation were 20 % (w/v) UV absorber content, 0.5 % (w/v) HPC addition concentration,
6 % (w/v) PEG addition concentration, 6 h agitation time, and 1000 rpm agitation speed. The particle distribution of microcapsules
manufactured under the appropriate conditions was 2–12 μm, and the average particle size was 6.27 μm. With regard to the thermal
characteristics of the microcapsules, the first peak appeared at 152 °C, with the second peak at 250 °C. Cotton-knitted fabrics
treated with silica microcapsules maintained an 80–90 % UV protection rate even after repeated washing. 相似文献
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Our study proposes an enzymatic scouring method for cotton fabrics using the enzyme cutinase. We established cutinase treatment
conditions for cotton fabrics from their relative activity at different pH levels, temperatures, enzyme concentrations, and
treatment times. Weight loss, moisture regain, K/S value, tensile strength, and SEM micrographs of cotton fabrics were analyzed. We determined the optimum cutinase treatment
conditions to be as follows: pH of 9.0, temperature of 50°C, cutinase concentration of 100 %, and a treatment duration time
of 60 min. We discovered that this cutinase treatment hydrolyzed the cuticle of cotton fabrics. The cutinase treatment did
not decrease the moisture regain and the K/S value. The optimum concentrations of Triton X-100 and calcium chloride, which were used as auxiliaries for cutinase treatment,
were found to be 0.5 % (v/v) and 70 mM, respectively. Some cracks were observed on the surface of the cotton fibers; however,
the tensile strength did not decrease. 相似文献
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In the next decade, advances in complementary metal-oxide semiconductor fabrication will lead to devices with gate lengths (the region in the device that switches the current flow on and off) below 10 nanometers (nm), as compared with current gate lengths in chips that are now about 50 nm. However, conventional scaling will no longer be sufficient to continue device performance by creating smaller transistors. Alternatives that are being pursued include new device geometries such as ultrathin channel structures to control capacitive losses and multiple gates to better control leakage pathways. Improvement in device speed by enhancing the mobility of charge carriers may be obtained with strain engineering and the use of different crystal orientations. Here, we discuss challenges and possible solutions for continued silicon device performance trends down to the sub-10-nm gate regimes. 相似文献