Effektivität sowie Stabilität des interspezifischen Chromosomen- und Gentransfers beim hexaploiden Weizen,Triticum aestivum L.     

Rolf Schlegel 《Genetic Resources and Crop Evolution》1990,38(1):67-78

Zusammenfassung Basierend auf experimentellen Ergebnissen wird demonstriert, daß chromosomale Manipulationen effektiv zur gezielten Übertragung von Fremdgenen in den hexaploiden Weizen genutzt werden können und zu züchterisch bzw. agronomisch verwertbaren Produkten führten. Die Stabilität der Integration von Chromosomen oder Chromosomensegmenten und die Expression der übertragenen Gene hängt von einer Vielzahl von cytologischen und genetischen Faktoren ab. Homöologe Austauschereignisse erweisen sich als besonders geeignet, obwohl auch nichthomöologe Substitutionen und Translokationen toleriert sowie beständig vererbt werden können. Die Vorteile unterschiedlicher Transferverfahren und die dabei auftretenden Probleme werden diskutiert.

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The efficiency and stability of interspecific chromosome and gene transfer in hexaploid wheat,Triticum aestivum L.

Summary The transfer of alien chromosomes and genes into wheat can be efficiently achieved by cytogenetic manipulations. They contributed to several substitution and translocation lines with improved resistance against diseases and abiotic stress, which are utilized in breeding programs. The stability of the insertion of alien chromosomes/segments and the expression of the alien genes is depending on numerous cytological and genetical factors. When an alien segment is transferred, the natural organisation of the genes is usually maintained intact. The genes not only can compensate for most of the replaced wheat genes, but they also interact well with their neighbouring recipient genes. Although nonhomoeologous recombinations occured, the homoeologous exchanges are the most dominating. If dosage effects are desired, the target segment can be introduced to additional homoeologous chromosomes. The advantages of different procedures of transfer and their limitations are discussed.

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Genetische Variabilität     

Joachim Dehne  Kurt Skiebe  Martin Stein  Hagen Herdam  Rolf Franke  Heinz Leike 《Genetic Resources and Crop Evolution》1988,36(1):247-274

Zusammenfassung Für die Pflanzenzüchtung ist die genetische Variabilität eine entscheidende Voraussetzung. Sie basiert auf Genmutationen, Chromosomenmutationen, Introgressionen, Autopolyploidie, Alloplasmie, transponiblen DNA-Elementen und der Kombination. Diese Variabilitätskomponenten werden charakterisiert. Außerdem wird anhand von Beispielen ihr Einfluß auf die Entwicklung der Kulturpflanzen demonstriert.Um effektiv in der Pflanzenzüchtung arbeiten zu können, bedarf es der Analyse von genetischer Variabilität. Dies geschieht mit Hilfe von Kreuzungsexperimenten und Parameterschätzungen. In zunehmendem Maße werden auch Marker bei der Analyse eingesetzt.Einen wichtigen Faktor zur zielgerichteten Beeinflussung der genetischen Variabilität stellt die Selektion dar. Sie führt zur Einschränkung der Kombination. Die Selektion ist für die Entwicklung von Sorten notwendig. Sie hat aber auch eine Verarmung an genetischer Information zur Folge.Für den weiteren Fortschritt in der Pflanzenzüchtung gilt es, eine genügende genetische Variabilität zu gewährleisten. Dazu bedarf es auch Maßnahmen zu ihrer Erhaltung. Genetische Variabilität muß außerdem verstärkt charakterisiert und analysiert werden.

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Genetic variability

Summary Genetic variability is a decisive prerequisite to plant breeding. It is based on gene mutations, chromosome mutations, introgressions, autopolyploidy, alloplasmy, transposible DNA elements and recombinations. These components of the genetic variability are illustrated and examples are given to demonstrate their contributions to the evolution of crop plants.Effective plant breeding requires the analysis of the genetic variation. It is analysed by hereditary studies and parameter estimations. The utilization of markers is continuously increasing in studies of the genetic variation.Selection is an important factor to a purposive influence on the genetic variability and leads to a restriction of the recombinations. Selection is necessary for the development of varieties but results also in an impoverishment of genetic information.For the continuous progress in plant breeding an adequate genetic variability is to secure. That includes also activities to its conservation. Furthermore the characterization and analysis of the genetic variability has to be intensified.

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Die Bestimmung des hydraulischen Gradienten an der Befeuchtungsfront und seine Bedeutung für die Tonablösung im Boden     

Rolf Lessing 《植物养料与土壤学杂志》1991,154(1):41-45

The determination of the hydraulic gradient of the wetting front and its relevance for clay detachment in soil As a result of some observations and experiments it must be supposed that clay migration does not only depend on the physico-chemical conditions but also on the water regime. Mechanical displacement of clay particles from its carrier can be the result of only rarely occuring high pore water velocities or of intensive changes of moisture suction at the wetting front, which can be quantified with the hydraulic gradient. A method how to determine the hydraulic gradient of the wetting front will be described. The determination and its importance for clay displacement will be explained by results of laboratory-experiments with sandy material packed into columns.  相似文献   

Precursors to damascenone: synthesis and hydrolysis of isomeric 3,9-dihydroxymegastigma-4,6,7-trienes     

Puglisi CJ  Daniel MA  Capone DL  Elsey GM  Prager RH  Sefton MA 《Journal of agricultural and food chemistry》2005,53(12):4895-4900

A series of four isomeric 3,9-dihydroxymegastigma-4,6,7-trienes, 8, has been prepared. The (3S,6R,9S) isomer of 8 proved to be identical to an isomer of this compound tentatively identified as an intermediate in the formation of damascenone from an allene triol. Each of the four isomers, when hydrolyzed independently of each other at pH 3.0 and 25 degrees C, produced product mixtures in which the major product was damascenone (1). Contrary to expectation, 3-hydroxydamascone (5) was not observed in any of the hydrolyses. Consequently, the mechanism of formation of damascenone proposed earlier requires modification. In each hydrolysis, the product mixtures showed the presence of a second isomer of 8, produced by epimerization during hydrolysis. Chiral analysis on a Cyclosil B column revealed that this epimerization was occurring at C(3) in each of the hydrolyses.  相似文献   

Microbial activity and bacterial composition of H₂-treated soils with net CO₂ fixation     

Susanne Stein  Rolf Schilling  Michael Schmid 《Soil biology & biochemistry》2005,37(10):1938-1945

The effects of H₂ gas treatment of an agricultural soil cultivated previously with a mixture of clover (Trifolium pratense) and alfalfa (Medicago sativa) on CO₂ dynamics and microbial activity and composition were analyzed. The H₂ emission rate of 250 nmol H₂ g⁻¹ soil h⁻¹ was similar to the upper limit of estimated H₂ amounts emitted from N₂ fixing nodules into the surrounding soil ([Dong, Z., Layzell, D.B., 2001. H₂ oxidation, O₂ uptake and CO₂ fixation in hydrogen treated soil. Plant and Soil 229, 1-12.]). After 1 week of H₂ supply to soil samples simultaneously with H₂ uptake net CO₂ production declined continuously and this finally led to a net CO₂ fixation rate in the H₂-treated soil of 8 nmol CO₂ g⁻¹ soil h⁻¹. The time course of H₂ uptake and CO₂ fixation in the soils corresponded with an increase in microbial activity and biomass of the H₂-treated soil determined by microcalorimetric measurements, fluorescence in situ hybridization analysis (FISH) and DNA staining (DAPI). Shifts in the bacterial community structure caused by the supply of H₂ were recorded. While the H₂ treatment stimulated β-and γ-subclasses of Proteobacteria, it had no significant effect on α-Proteobacteria. In addition, FISH-detectable bacteria of the Cytophaga-Flavobacterium-Bacteroides phylum increased in numbers.  相似文献   

Die Rolle der mikrobiellen Biomasse und des mineralisch fixierten Ammoniums bei den Stickstoff-Transformationen in niedersächsischen Löß-Ackerböden unter Winter-Weizen. I. Poolgrößenveränderungen     

Rolf Nieder  Elisabeth Neugebauer  Astrid Willenbockel  Jrg Richter 《植物养料与土壤学杂志》1995,158(5):469-475

Significance of microbial biomass and non-exchangeable ammonium with respect to the nitrogen transformations in loess soils of Niedersachsen during the growing season of winter wheat. I. Change of pool sizes Nitrogen transformations in loess soils have been examined by laboratory and field experiments. After straw application (· 8 t · ha^?1), N in microbial biomass (N_mic) increased by about 20 mg · kg^?1 soil (· 90 kg N · ha^?1 · 30 cm^?1) after 9 days of incubation (20 °C). Another laboratory experiment yielded an increase of about 400 mg of NH₄+-N · kg^?1 fixed by minerals within 1 h after addition of 1 M NH₄+-acetate. Defixation of the recently fixed NH₄+ after addition of 1 M KCl amounted to only 60 mg · kg^?1 within 50 days. In a field experiment with winter wheat 1991, an increase in N_mic of about 80 kg N · ha^?1 · 30 cm^?1 was observed from March to June. After July, growth of the microbes was limited by decreased soluble carbon concentrations in the rhizosphere. Different levels of mineral N-fertilizer (0, 177 and 213 kg N · ha^?1) did not affect significantly the microbial biomass. The same field experiment yielded a decrease of non-exchangeable ammonium on the “zero”-fertilized plot in spring by 200 kg N · ha^?1 · 30 cm^?1. The pool of fixed ammonium increased significantly after harvest. After conventional mineral N-fertilizer application (213 kg N · ha^?1). NH₄+-defixation was only about 120 kg N · ha^?1 · 30 cm^?1 until July.  相似文献   

Effects of mustard residues on the carbon and nitrogen turnover in undisturbed soil microcosms     

Ralph E. Hantschel  Eckart Priesack  Rolf Hoeve 《植物养料与土壤学杂志》1994,157(5):319-326

The C- and N-turnover of undisturbed, irrigated 25 cm soil microcosms of a Typic Agrudalf from loess were followed during a laboratory incubation of about 200 days. Besides the untreated control columns two treatments were installed to test the effects of the application of white mustard (Sinapis alba L.) on both nutrient cycles (either mixing the dried plant residues to 0–8 cm or surface application). Each of the treatments is represented by 4 soil columns to account for soil variability. The C-budget was mainly altered for soil respiration, which increased for the microcosms treated with mustard rapidly after application. After 4 weeks no differences could be proved however. The loss of soluble C-compounds, which was unimportant for the budget, reflected no one of the treatments with mustard. Net N mineralization, as calculated from the NO₃-leaching, could be well fitted by zero-order kinetics. Although the high variability of the net N mineralization of the replicates mustard application significantly rises the N-release. No difference could be proved for the different techniques of application however. In the mixed treatment 38% of the nitrogen added with the mustard was decomposed during the incubation period, in the surface treatment 34%.  相似文献   

Ergebnisse serologischer und bakteriologischer Untersuchungen von Hundeblutproben auf Brucella canis     

Rolf Baron von Kruedener 《Zoonoses and public health》1976,23(7):555-565

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Ten years of bovine virus diarrhoea virus (BVDV) control in Norway: a cost-benefit analysis   总被引：1，自引：0，他引：1  

Valle PS  Skjerve E  Martin SW  Larssen RB  Østerås O  Nyberg O 《Preventive veterinary medicine》2005,72(1-2):189-207; discussion 215-9

A retrospective cost-benefit analysis was carried out on the Norwegian bovine virus diarrhoea (BVD) control and eradication strategy, for the years 1993-2003. Information regarding the control cost input parameters was gathered from the cattle industry (TINE Norwegian Dairies, GENO Breeding and AI association, and GILDE Norwegian Meat), The National Animal Health Authorities and The Veterinary Institute. We accounted for variable costs (both direct costs associated with the control, and those costs carried by the farmers as a consequence of the control program). The benefit was estimated as the difference between the assumed losses without control - represented overall as 10% increase of the observed 1993 BVD virus infection level - and the observed losses during the control period. An estimate of the financial losses associated with the BVD virus (BVDV) infection was based on studies of the herd level effects on health, reproduction, and production in dairy herds with evidence of recent BVDV infection. We used a stochastic simulation model to account for the total uncertainty in both the control cost and financial loss estimates. The annual net benefits over the 10 years of BVD control were discounted to a 1993 net present value (NPV). The median NPV of the BVD control, nationally, was estimated at 130 million NOK with a distribution of the NPV ranging from +51 to +201 million NOK (5th and 95th percentiles, respectively). Out of the total control costs the farmers and the farmer-owned industries (the co-operatives) had carried about 62% of these costs; however, the farmers were also the main beneficiaries. The Norwegian experience shows a robust cost-efficiency for a BVDV eradication strategy; this stands in sharp contrast to earlier studies where the results were not supportive. Even though every cattle population and country is unique, the Norwegian findings and experiences should have wider implications.  相似文献   

Intraepithelial but not lamina propria lymphocytes in the porcine gut are affected by dexamethasone treatment     

Schwarz E  Saalmüller A  Gerner W  Claus R 《Veterinary immunology and immunopathology》2005,105(1-2):125-139

It is well established that glucocorticoids are key regulators of the immune system and act as immunosuppressive agents in high concentrations. In the pig, effects on the gut immune system and trafficking of lymphocytes between tissues and blood plasma were not investigated so far. Twelve pigs of 70 kg were fed 0.4 mg portions of dexamethasone (Dexa) twice daily for 9 days or remained untreated (controls) and were sacrificed for tissue collection at the end of Dexa treatment. Another six pigs with jugular vein catheters were left untreated for 7 days (control period) and then received Dexa for 9 days. Blood was drawn twice during the control period and at days 3, 6 and 9 of the Dexa period for characterization of peripheral blood leukocytes. Cells were obtained from thymus, mesenteric lymph nodes, jejunal mucosa and Peyer's patches. Lymphoid cells from gut tissue were isolated from two fractions: the EDTA-fraction, containing the intraepithelial lymphocytes (IEL), and the Collagenase-fraction, containing the lamina propria lymphocytes (LPL). In all samples, cell counts and phenotypic characterization of cells by flow cytometry (FCM) were performed. In thymus, Dexa led to a more than 90% reduction of the absolute cell number, which was mainly found in the CD4+CD8+ subpopulation. Dexa effects on lymphocytes from mesenteric lymph nodes were less severe (50%) and led mainly to a decrease (71%) of B-lymphocytes. The number of lymphocytes in the EDTA-fraction (IEL) of the jejunal mucosa decreased significantly by 56% in the Dexa-treated animals compared to the controls, whereas the number of lymphocytes in the Collagenase-fraction (LPL) decreased only moderately. In the Peyer's patches, a decreasing tendency in the number of lymphocytes in the EDTA-fraction was observed which, however, was not significant. In blood, monocytes and granulocytes were significantly increased in an order of 60%. The data show that supraphysiological amounts of Dexa remarkably reduce cell numbers in thymus and also in the intraepithelial compartment of the jejunal mucosa and ileal Peyer's patches. In blood, a notable homeostasis was observed for several leukocyte populations whereas both monocytes and granulocytes increased.  相似文献