Intracardiac heartworms in dogs: Clinical and echocardiographic characteristics in 72 cases (2010‐2019)     

Abigail E. Romano  Ashley B. Saunders  Sonya G. Gordon  Sonya Wesselowski 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):88-97

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Sodium distribution in the bovine brain     

Megan C. Romano  Grace E. Higgs  Michelle N. Helm  Darko Stefanovski  Cynthia L. Gaskill 《Journal of veterinary diagnostic investigation》2021,33(2):384

Fatal sodium intoxication can occur in many species, including cattle, and postmortem confirmation often includes brain sodium concentration determination. Published information regarding brain sodium distribution in cattle was not found in a literature review. Our study was designed to determine whether sodium is uniformly distributed throughout the bovine brain. Eight whole bovine brains were collected from adult cattle with no neurologic signs or history suggestive of sodium intoxication, and with a non-neurologic cause of death diagnosed on gross examination. Brains were divided mid-sagittally. One hemisphere of each brain was homogenized. Subsamples were obtained from the remaining hemisphere (rostral, caudal, and dorsal cerebral cortices; brainstem, thalamus, and cerebellum). Sodium concentrations of regions and homogenates were measured by inductively coupled plasma–mass spectrometry. Data were analyzed using repeated measures ANOVA with a pairwise post-test to compare mean sodium concentration of each region to mean homogenate sodium concentration. Brain sodium was not uniformly distributed; sodium concentrations in different regions of the same brain varied somewhat unpredictably. Homogenization of an entire brain hemisphere appears to be the ideal method of sample preparation to ensure accurate brain sodium concentration measurement in adult cattle.  相似文献   

Comparison between two approaches for the transversus abdominis plane block in canine cadavers     

Marta Romano  Diego A. Portela  Alexander Thomson  Pablo E. Otero 《Veterinary anaesthesia and analgesia》2021,48(1):101-106

ObjectiveTo compare the dye distribution following either two lateral abdominal or one lateral abdominal and one subcostal ultrasound-guided transversus abdominis plane (TAP) injections of a clinically relevant volume of dye solution in dogs.Study designRandomized cadaveric study.AnimalsA total of eight canine cadavers.MethodsOn one side of each cadaver, two TAP injections were performed on the lateral aspect of the abdomen (approach LL), caudal to the last rib and cranial to the iliac crest. On the contralateral hemiabdomen, one subcostal (caudal to the costal arch) and one lateral abdominal injection (between last rib and iliac crest), were performed (approach SL). Side allocation was randomly determined. A spinal needle was introduced in-plane to the transducer for each injection of methylene blue (0.25 mL kg^?1). All cadavers were dissected to assess dye distribution and number of stained target nerves.ResultsAll injections were performed in the TAP. The proportion of target nerve staining was 53.5% versus 80.4% with approaches LL and SL, respectively (p = 0.005). Approach LL stained the first lumbar (L1) spinal nerve in 100% of injections and ninth thoracic (T9), T10, T11, T12, T13 and L2 were stained in 0%, 0%, 37.5%, 62.5%, 87.5% and 87.5% of injections, respectively. Approach SL stained T11, L1 and L2 in 100% of injections and T9, T10, T12 and T13 were stained in 37.5%, 87.5%, 75% and 62.5% of injections, respectively. Approach SL resulted in greater staining of nerves cranial to T12 compared with approach LL. The two approaches were equivalent in staining nerves caudal to T12.Conclusions and clinical relevanceApproach SL provided a broader distribution of the injected solution than approach LL, which may result in a larger blocked area in live animals undergoing celiotomy.  相似文献   

Report on drugs, narcotic and dangerous     

A Romano 《Journal of the Association of Official Analytical Chemists》1974,57(2):256-258

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Floral Analysis and Seasonal Dynamics of Mineral Levels in Carob Tree Leaves     

L. Custódio  P. J. Correia  M. A. Martins-Loução  A. Romano 《Journal of plant nutrition》2013,36(5):739-753

The mineral concentration of flowers and the seasonal fluctuation of macro- [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)] and micronutrients [iron (Fe), manganese (Mn), zinc (Zn), and copper (Cu)] in leaves of male, female, and hermaphroditic carob trees (Ceratonia siliqua L.) were studied. The nutrient dynamics were linked not only to phenological events, but also to the gender of the trees. The females were able to allocate more nutrients to leaves than male trees, even though male flowers were richer in particular elements such as N and Zn. The hermaphrodites supported the development of both inflorescences and fruits with a lower seasonal variation and a lower leaf nutrient concentration, as compared to the other genders, which may indicate a more efficient use of resources. Flowers had, in general, a higher concentration of N, P, and K, and a lower Mn concentration than in leaves. Flowers of the females had a lower nutrient concentration compared to males or hermaphrodites.  相似文献   

The wheat AMT2 (AMmonium Transporter) family,possible functions in ammonium uptake and pathogenic/symbiotic interactions     

Romano Porras-Murillo  Yufen Zhao  Jinling Hu  Toyosi Ijato  Joseline Palafox Retamal  Uwe Ludewig  Benjamin Neuhäuser 《植物养料与土壤学杂志》2023,186(2):164-168

Ammonium uptake into wheat roots relies primarily on two AMmonium Transporters of subfamily one, while the wheat genome comprises 4 to 6 AMT2 type transporters. Plant AMT2s generally show functions in root-to-shoot translocation or pathogenic and symbiotic plant–microorganism interactions. We addressed the activity of TaAMT2s in ammonium transport. Nitrogen-dependent expression implicated a physiological function in ammonium uptake for TaAMT2;1 and in ammonium distribution for TaAMT2;2-6.  相似文献   

The effects of salinity on the survival, growth and haemolymph osmolality of early juvenile blue swimmer crabs, Portunus pelagicus     

Nicholas Romano  Chaoshu Zeng 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):151-162

The blue swimmer crab, Portunus pelagicus, is an emerging aquaculture species in the Indo-Pacific. Two experiments were performed to determine the effects of salinity on survival, growth and haemolymph osmolality of early juvenile P. pelagicus crabs. The salinities tested for the first experiment were 10, 15, 25 and 40 ppt, and for the second experiment 5, 20, 30, 35 and 45 ppt. Each salinity experiment was triplicated, with each replicate consisting of 10 stage 4 juveniles. Each experiment lasted 45 days. Mortalities and incidence of “molt death syndrome” were recorded daily, while the intermolt period, carapace length, carapace width and wet weight were measured at each molt. At the end of the experiments the haemolymph osmolality and dry weights were measured.

Results demonstrate that salinity significantly affects both the survival and growth of early P. pelagicus juveniles. Mortality was significantly higher (p < 0.01) for juveniles cultured at salinities ≤ 15 ppt and at 45 ppt. At a salinity of 5 ppt a complete mortality occurred on day 20. In all salinity treatments, the majority of mortalities were due to “molt death syndrome”. In experiment 1, immediate effects of salinity on growth and development were seen at 10 ppt as the intermolt period was significantly longer (p < 0.01) and the mean carapace size increase was significantly less (p < 0.01) at the first molt compared to the other treatments. Meanwhile, the specific growth rates (carapace length, width and wet weight) were significantly lower (p < 0.05) at high salinities (≥ 40 ppt) due to longer intermolt periods and significantly lower (p < 0.05) carapace size or wet weight increases.

The haemolymph osmolality exhibited a positive linear relationship with the culture medium with an isosmotic point of 1106 mOsm/kg, equal to a salinity of approximately 38 ppt. Based on the osmolality graph, high metabolic cost for osmoregulation due to increased hyper- and hypo-osmotic stress appeared to cause lower survival and specific growth rates of the crabs. The results demonstrate that a salinity range of 20–35 ppt is suitable for the culture of early juvenile P. pelagicus.  相似文献   

Benthic foraminifera as proxies of marine influence in the Orosei marine caves,Sardinia, Italy     

Elena Romano  Luisa Bergamin  Letizia Di Bella  Virgilio Frezza  Andrea Marassich  Giancarlo Pierfranceschi  Claudio Provenzani 《水产资源保护:海洋与淡水生态系统》2020,30(4):701-716

1. As a result of their location at the boundary between marine and continental domains, marine caves are affected by wide spatial and seasonal environmental changes. Only recently have benthic foraminifera been recognized as reliable indicators for the ecological zonation of these environments.
2. The present study is focused on two marine caves of the Orosei Gulf, Sardinia, Italy: Bue Marino and Bel Torrente. It investigates the spatial and seasonal variability of benthic foraminiferal assemblages relative to sediment grain size and water parameters (temperature, salinity, pH, and dissolved oxygen) collected during two campaigns in August 2014 and April 2015. The results from 2014 have been partially published.
3. Based on a comparison of the results of the two campaigns, the considerable reduction of foraminiferal abundance in Bel Torrente was deduced to occur because of the strong freshwater flows occurring during the rainy season; in Bue Marino, the less severe water flow allowed the identification of both living and dead foraminifera, although strongly reduced in number. These identifications allowed benthic foraminifera to be used to define the ecological zonation.
4. Entrance, confluence, and transitional ecozones were identified in Bue Marino cave on the basis of species abundance. The second ecozone, not recognized in 2014, was correlated with plant debris at the confluence of the two cave branches. The other two ecozones, which are characterized by the faunal shift from hyaline‐ to agglutinated‐prevalent assemblages, were attributed to the gradient of abiotic parameters detected from the outer to the inner portions of the cave. In both campaigns the same ecozones were recognized in terms of species composition, with exceptions being found to different extents as a result of seasonal variability.
5. As the distribution of foraminiferal ecozones is conditioned by a decreasing gradient of marine influence, long‐term monitoring may be regarded as a promising tool for future studies on sea‐level change.

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Diet composition of the Italian crested newt (Triturus carnifex) in structurally different artificial ponds based on stomach contents and stable isotope analyses     

Giulio Careddu  Nicol Carlini  Antonio Romano  Loreto Rossi  Edoardo Calizza  Simona Sporta Caputi  Maria Letizia Costantini 《水产资源保护:海洋与淡水生态系统》2020,30(8):1505-1520

1. In order to evaluate the effects of ecological disturbances, such as climate change, human‐induced habitat modification, or species introduction, and in order to adopt appropriate management policies for their conservation, knowledge of the trophic ecology of protected or threatened species is crucial. The Italian crested newt, Triturus carnifex (Laurenti, 1768), is listed in annexes II and IV of the European Habitats Directive. For this species, changes in water quality and habitat loss represent major threats, potentially impairing its breeding and feeding activities.
2. Artificial aquatic habitats, such as cattle‐drinking pools, represent valuable refuges and suitable breeding sites for T. carnifex. The aim of this study was to determine the food niche of the Italian crested newt in these habitats, evaluating its prey selection strategy in relation to a range of environmental conditions and differing availability of resources. Stomach contents (indicative of short‐term diet) and δ¹³C and δ¹⁵N signatures of tails (indicative of medium‐term diet) of adult newts from three artificial ponds in central Italy, where traditional silvo‐pastoral activities are still important, were determined. Potential prey were also sampled and processed for stable isotope analysis.
3. Triturus carnifex, similar to other newt species, is an opportunistic predator, feeding on a wide variety of prey that includes terrestrial and aquatic macroinvertebrates. In particular, the opportunistic use of temporally available small zooplankton was identified from stomach contents analysis. Conversely, stable isotope analysis highlighted the consumption of larger, soft‐bodied prey that the stomach‐contents‐based approach underestimated. These included terrestrial oligochaetes, which turned out to be the most important and energy‐rich food source in the medium term.
4. These results emphasize the usefulness of combined approaches for studying the trophic ecology of salamanders and also highlight the importance of the integrated management of aquatic breeding habitats and neighbouring terrestrial habitats, as sources of food, for newt conservation.

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Retrospective clinical evaluation of ultrasound guided transverse abdominis plane block in dogs undergoing mastectomy     

Diego A. Portela  Marta Romano  Angela Briganti 《Veterinary anaesthesia and analgesia》2014,41(3):319-324

HistoryEleven female dogs of different breeds undergoing unilateral radical (n = 7) or regional abdominal mastectomy (n = 4) received an ultrasound guided transverse abdominis plane block (TAP-block).Physical examinationSubjects showed single or multiple mammary tumours. Serum biochemistry, CBC and electrocardiogram were unremarkable. Eight animals were classified as ASA physical status II and 3 as ASA III.ManagementDogs were premedicated with methadone [0.1 or 0.2 mg kg^?1 intravenously (IV) or intramuscularly respectively] or fentanyl (2.5 μgkg^?1 IV). Anaesthesia was induced with propofol and maintained with isoflurane or sevoflurane. Unilateral ultrasound guided TAP blocks were performed in the caudal and cranial abdomen with bupivacaine 0.25% (0.3 to 0.35 mL kg^?1). Intercostal nerve blocks (T₄ to T₁₁) with bupivacaine 0.25% (0.013 to 0.04 mL kg^?1) completed the blocked area in dogs undergoing radical mastectomy.Follow upThe median (range) of end-expired isoflurane and sevoflurane necessary to maintain anaesthesia was 1.15 (1.07–1.22) and 2.07 (2.05–2.2) vol% respectively. A single administration of fentanyl (2.5 μg kg^?1, IV) was administered to control nociception (defined as an increased heart rate or mean arterial blood pressure above 20% of the pre-incisional value) in four of 11 dogs. All dogs received carprofen (2 mg kg^?1 subcutaneously) at the end of surgery. Post-operative pain, assessed for 120 minutes using the short form of Glasgow Composite Pain Scale (0–24), was always lower than 3. No rescue analgesia (allowed by the protocol) was required in this time.ConclusionTransverse abdominis plane block combined with intercostal nerve blocks may be useful to produce intraoperative anti-nociception and short term post-operative analgesia in dogs undergoing unilateral mastectomy.  相似文献