A review of the roles of keratinocyte‐derived cytokines and chemokines in the pathogenesis of atopic dermatitis in humans and dogs          下载免费PDF全文

Ryota Asahina  Sadatoshi Maeda 《Veterinary dermatology》2017,28(1):16-e5

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Diversity of fimbrillin among Porphyromonas gulae clinical isolates from Japanese dogs     

Nomura R  Shirai M  Kato Y  Murakami M  Nakano K  Hirai N  Mizusawa T  Naka S  Yamasaki Y  Matsumoto-Nakano M  Ooshima T  Asai F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(7):885-891

Porphyromonas gulae, a gram-negative black-pigmented anaerobe, is a pathogen for periodontitis in dogs. An approximately 41-kDa fimbrial subunit protein (FimA) encoded by fimA is regarded as associated with periodontitis. In the present study, the fimA genes of 17 P. gulae strains were sequenced, and classified into two major types. The generation of phylogenetic trees based on the deduced amino acid sequence of FimA of P. gulae strains along with sequences from several strains of Porphyromonas gingivalis, a major cause of human periodontitis, revealed that the two types of FimA (types A and B) of P. gulae were similar to type I FimA and types II and III FimA of P. gingivalis, respectively. A PCR system for classification was established based on differences in the nucleotide sequences of the fimA genes. Analysis of 115 P. gulae-positive oral swab specimens from dogs revealed that 42.6%, 22.6%, and 26.1% of them contained type A, type B, and both type A and B fimA genes, respectively. Experiments with a mouse abscess model demonstrated that the strains with type B fimA caused significantly greater systemic inflammation than those with type A. These results suggest that the FimA proteins of P. gulae are diverse with two major types and that strains with type B fimA could be more virulent.  相似文献   

Involvement of endogenous vasopressin in high plasma osmolality-induced anorexia via V1 receptor-mediated mechanism     

Ikemura R  Matsuwaki T  Yamanouchi K  Nishihara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):951-955

It is known that water deprivation or injection of hypertonic saline induces anorexia. The present study examined the possible involvement of vasopressin in the suppression of food intake during high plasma osmolality. Intraperitoneal injection of vasopressin (20 microg/kg) into male rats significantly suppressed food intake for 1 hr. This anorectic effect of vasopressin was reversed by simultaneous injection of a peptide antagonist for V(1) receptor (40 microg/kg), but not for V(2) receptor (40 microg/kg). Intraperitoneal injection of hypertonic saline (20% NaCl, 2 ml/kg) similarly suppressed food intake for 2 hr, which was associated with a transient increase in plasma vasopressin concentrations. This hypertonic saline-induced suppression of food intake was blocked by a V(1) receptor antagonist. Vasopressin (40 ng/2 microl) directly administered into the third ventricle of the brain also suppressed food intake for 1 hr. These results suggest that vasopressin participates in the suppression of food intake during high plasma osmolality, the action of which is mediated by V(1) receptors in the brain.  相似文献   

Pathological studies on the ectopic migration of Dirofilaria immitis in the brain of dogs (author's transl)]     

T Kotani  T Tomimura  M Ogura  H Mochizuki  M Horie 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1975,37(2):141-154

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Transcriptional analysis of the IL‐33 receptor suppression of tumourigenicity 2 and its effects on canine Type 2 T helper cells: a preliminary study          下载免费PDF全文

Ryota Asahina  Hidetaka Nishida  Hiroaki Kamishina  Sadatoshi Maeda 《Veterinary dermatology》2018,29(2):112-e45

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The combined effect of clothianidin and environmental stress on the behavioral and reproductive function in male mice     

Tetsushi HIRANO  Shogo YANAI  Takuya OMOTEHARA  Rie HASHIMOTO  Yuria UMEMURA  Naoto KUBOTA  Kiichi MINAMI  Daichi NAGAHARA  Eiko MATSUO  Yoshiko AIHARA  Ryota SHINOHARA  Tomoyuki FURUYASHIKI  Youhei MANTANI  Toshifumi YOKOYAMA  Hiroshi KITAGAWA  Nobuhiko HOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1207-1215

Neonicotinoids, some of the most widely used pesticides in the world, act as agonists to the nicotinic acetylcholine receptors (nAChRs) of insects, resulting in death from abnormal excitability. Neonicotinoids unexpectedly became a major topic as a compelling cause of honeybee colony collapse disorder, which is damaging crop production that requires pollination worldwide. Mammal nAChRs appear to have a certain affinity for neonicotinoids with lower levels than those of insects; there is thus rising concern about unpredictable adverse effects of neonicotinoids on vertebrates. We hypothesized that the effects of neonicotinoids would be enhanced under a chronic stressed condition, which is known to alter the expression of targets of neonicotinoids, i.e., neuronal nAChRs. We performed immunohistochemical and behavioral analyses in male mice actively administered a neonicotinoid, clothianidin (CTD; 0, 10, 50 and 250 mg/kg/day), for 4 weeks under an unpredictable chronic stress procedure. Vacuolated seminiferous epithelia and a decrease in the immunoreactivity of the antioxidant enzyme glutathione peroxidase 4 were observed in the testes of the CTD+stress mice. In an open field test, although the locomotor activities were not affected, the anxiety-like behaviors of the mice were elevated by both CTD and stress. The present study demonstrates that the behavioral and reproductive effects of CTD become more serious in combination with environmental stress, which may reflect our actual situation of multiple exposure.  相似文献   

Gene transcription of pro‐inflammatory cytokines and chemokines induced by IL‐17A in canine keratinocytes          下载免费PDF全文

Ryota Asahina  Harumi Kamishina  Hiroaki Kamishina  Sadatoshi Maeda 《Veterinary dermatology》2015,26(6):426-e100

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New cylindrocladium diseases of strawberry and coral bells caused by <Emphasis Type="Italic">Cylindrocladium canadense</Emphasis>     

Yuuri Hirooka  Seiju Ishikawa  Jun Takeuchi  Hiromichi Horie  Kiichi Nakayama  Motoo Koitabashi  Seiichi Okuda  Keiko T. Natsuaki 《Journal of General Plant Pathology》2009,75(1):83-86

Based on inoculation experiments and morphological and molecular phylogenetic studies, Cylindrocladium damping-off of strawberry and Cylindrocladium blight of coral bells in Japan were demonstrated to be caused by Cylindrocladium canadense. This is the first report of a disease on strawberry and coral bells caused by this fungus.  相似文献   

Establishment of a model for chemoattraction of <Emphasis Type="Italic">Symbiodinium</Emphasis> and characterization of chemotactic compounds in <Emphasis Type="Italic">Acropora tenuis</Emphasis>     

Ryota Takeuchi  Mitsuru Jimbo  Fumika Tanimoto  Chiaki Tanaka  Saki Harii  Yoshikatsu Nakano  Ko Yasumoto  Shugo Watabe 《Fisheries Science》2017,83(3):479-487

Corals harbor symbiotic dinoflagellates, Symbiodinium spp., acquired from surrounding environments. Because Symbiodinium are present at low densities in the water column, corals may attract these symbionts using chemotactic compounds. To examine whether corals contain chemotactic compounds, we established an assay to measure the chemotactic activity for Symbiodinium using an extract of the coral Acropora tenuis, a major reef-building coral in Japan. Our assay revealed that Symbiodinium strain NBRC102920 (clade A), which is taken up by juvenile A. tenuis polyps, is attracted to crude A. tenuis extracts. We found that the chemotactic compounds are water-soluble, heat-labile macromolecules and that the chemotactic activity was inhibited by N-acetyl-d-glucosamine (GlcNAc). We separated the GlcNAc-binding fraction (Fr-ActL) and identified it as the most plausible candidate for the chemoattractant, since the chemotactic activity of the crude A. tenuis extract appeared to be mainly attributable to the activity of Fr-ActL and was also inhibited by the addition of GlcNAc. These results indicate that chemoattraction is mediated via the binding of Symbiodinium to Fr-ActL.  相似文献   

A preliminary experiment regarding the natural induction of gonadal development in female Japanese eels without hormone treatment     

Naomi Mikawa  Yoshiaki Yamada  Noriyuki Horie  Akihiro Okamura  Tomoko Utoh  Satoru Tanaka  Katsumi Tsukamoto 《Aquaculture Research》2019,50(12):3749-3754

To test whether gonadal development of female eels could be promoted without any exogenous hormone treatments, we observed the effect of water temperature manipulation. After 3–5 months of water temperature treatments, three silver eels showed higher gonadosomatic indices (GSI). In particular, one eel in the 5–15°C fluctuating temperature treatment group (5°C daytime and 15°C night‐time) had the highest GSI of 8.5 with secondary yolk globule stage oocytes of large diameter (OD; 412 μm) after 3 months, which indicated definite gonadal development compared with those in the initial states (mean GSI, 2.4; OD, 226.7 μm). The 5–15°C fluctuating temperature and constant 5°C groups had low oocyte breakdown (atretic) rates. Because daily temperature fluctuations and cold daytime water are experienced by vertically migrating silver eels in the ocean, these temperature conditions may be key maturation process components that could be useful for hormone‐free artificial maturation.  相似文献