Absorption and translocation of nitrogen in cucumber (Cucumis sativus L.) plants using the ¹⁵N tracer technique     

Ryota TANEMURA  Hiroshi KURASHIMA  Norikuni OHTAKE  Kuni SUEYOSHI  Takuji OHYAMA 《Soil Science and Plant Nutrition》2008,54(1):108-117

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Depth profiling of photodegraded wood surfaces by confocal Raman microscopy     

Toru Kanbayashi  Yutaka Kataoka  Atsuko Ishikawa  Masahiro Matsunaga  Masahiko Kobayashi  Makoto Kiguchi 《Journal of Wood Science》2018,64(2):169-172

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In situ observation of pinewood nematode in wood     

Toshihiro Umebayashi  Toshihiro Yamada  Kazunari Fukuhara  Ryota Endo  Dai Kusumoto  Kenji Fukuda 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(2):463-467

In pine wilt disease, xylem dysfunction occurs in relation to nematode migration and proliferation in host tissue, but the detection accuracy of pinewood nematode (PWN), Bursaphelenchus xylophilus, in pine stem tissue remains unclear. This study describes the use of cryo-scanning electron microscopy (cryo-SEM) and fluorescein-conjugated wheat germ agglutinin (F-WGA) staining to detect PWN. After PWN invasion, the frequency of surface fluorescence in PWN increased in pine stems from the day of inoculation to 3 weeks after inoculation. However, the fluorescence frequency decreased significantly during the advanced disease stage after 5 weeks. Thus, detecting PWN based on fluorescent staining of the nematode body surface coat protein can be misleading when used to examine the correlation between the development of disease symptoms and the nematode population. In contrast, all cut body segments were fluorescent, and their fluorescent components were common in pine-stem cross sections, regardless of the timing after inoculation. In addition, PWN were observed under cryo-SEM only in empty resin canals and this distribution was confirmed by F-WGA staining of PWN cut in a cross section. Thus, PWN detection based on fluorescent staining of surface coat proteins by F-WGA was not reliable in longitudinal sections of pine stems because of changes in nematode stainability during disease progression. To detect PWN in infected plants, we concluded that a combination of both methods is most effective.  相似文献   

Analysis of Host Species Specificity of Magnaporthe grisea Toward Foxtail Millet Using a Genetic Cross Between Isolates from Wheat and Foxtail Millet     

Murakami J  Tomita R  Kataoka T  Nakayashiki H  Tosa Y  Mayama S 《Phytopathology》2003,93(1):42-45

ABSTRACT Host species specificity of Magnaporthe grisea toward foxtail millet was analyzed using F(1) cultures derived from a cross between a Triticum isolate (pathogenic on wheat) and a Setaria isolate (pathogenic on foxtail millet). On foxtail millet cvs. Beni-awa and Oke-awa, avirulent and virulent cultures segregated in a 1:1 ratio, suggesting that a single locus is involved in the specificity. This locus was designated as Pfm1. On cv. Ki-awa, two loci were involved and one of them was Pfm1. The other locus was designated as Pfm2. Interestingly, Pfm1 was not involved in the pathogenic specificity on cv. Kariwano-zairai. These results suggest that there is no "master gene" that determines the pathogenic specificity on all foxtail millet cultivars and that the species specificity of M. grisea toward foxtail millet is governed by cultivar-dependent genetic mechanisms that are similar to gene-for-gene interactions controlling race-cultivar specificity.  相似文献   

Determination of pyrimethamine and sulphadimethoxine residues in eggs by high performance liquid chromatography.     

T Nagata  M Saeki  T Iida  M Kataoka  S Shikano 《British poultry science》1992,33(5):953-961

1. Laying hens were given food containing per kg diet: 1 mg pyrimethamine, 10 mg sulphadimethoxine (SDM) or 1 mg pyrimethamine with 10 mg SDM, for 14 days. Residues in the eggs were determined by high performance liquid chromatography. 2. Pyrimethamine was mainly found in yolk. Its concentration was slightly higher when it was given with SDM. 3. The concentrations of SDM in eggs were not different when it was given alone or in combination with pyrimethamine. 4. After withdrawal, pyrimethamine residues decreased below the detection limit (0.02 mg/kg) in the yolk after 11 to 12 d and in the albumen after 2 to 3 d. SDM in yolk fell below the detection limit (0.01 mg/kg) on day 7, whereas in albumen it was not detectable after 2 to 3 d.  相似文献   

Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido     

Kataoka N  Nishimura M  Horiuchi M  Ishiguro N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):349-351

Surveillance of chronic wasting disease (CWD) was conducted by performing Western blot analysis of tissue samples from 136 sika deer (Cervus nippon) killed by hunters in the Tokachi district of Hokkaido Island. No prion protein (PrPSc) associated with CWD was detected in any of the samples. To assess amino acid polymorphisms of the sika deer PrP gene, nucleotide sequencing of the PrP gene was performed. The only amino acid polymorphisms detected were 3 silent mutations at nucleotide positions 63, 225 and 408. These results suggest that sika deer in the Tokachi district are genetically homogeneous, and are not infected with CWD.  相似文献   

Pathogenicity for mice and swine of Erysipelothrix isolates from the tonsils of healthy cattle     

Hassanein R  Sawada T  Kataoka Y  Gadallah A  Suzuki Y  Takagi M  Yamamoto K 《Veterinary microbiology》2003,91(2-3):231-238

The pathogenicity of 79 Erysipelothrix isolates from bovine tonsils for mice and swine was determined. Five (6.3%) isolates were lethal for mice. These isolates belonged to serovars 1b (one isolate), 2 (2), 19 (1) and 21 (1). The 50% lethal dose values of the isolates ranged from 0.33 to 5x10(2) CFUs in mice. Twenty Erysipelothrix isolates (25.3%) were weakly virulent inducing only emaciation while 12 (15.2%) inducing emaciation and ruffled hair. In swine, clinical signs of varying severity were observed. Four isolates were virulent, capable of inducing localized or generalized urticarial lesions accompanied with a rise in body temperature after intradermal inoculation. One isolate each of serovars 1b, 2 and 19 was highly virulent, capable of inducing generalized urticarial lesions while another Erysipelothrix isolate of serovar 2 induced only a localized urticarial lesion at the site of inoculation. Another isolate of serovar 1b induced itching and irritation without obvious urticarial lesion at the site of inoculation. On the other hand, one isolate of serovar 21 and two other isolates of serovar 2 could not induce experimentally any clinical sign of erysipelas other than rise in body temperature. There was a rise in growth agglutination (GA) titer of serum in all the inoculated swine. These observations suggest that Erysipelothrix isolates from cattle are pathogenic for mouse and swine, and may also be pathogenic for other animals and humans.  相似文献   

Retrospective serosurvey for feline immunodeficiency virus infection in Japanese cats   总被引：2，自引：0，他引：2  

T Ishida  A Taniguchi  T Kanai  Y Kataoka  K Aimi  K Kariya  T Washizu  I Tomoda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1990,52(2):453-454

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Detection of Streptococcus suis type 2 in tonsils of slaughtered pigs using improved selective and differential media   总被引：2，自引：0，他引：2  

Y Kataoka  C Sugimoto  M Nakazawa  M Kashiwazaki 《Veterinary microbiology》1991,28(4):335-342

New selective and differential media were devised for the isolation and identification of Streptococcus suis type 2. The selective medium (NNCC) agar) was Todd-Hewitt broth containing 1.5% Bactoagar and 5% defibrinated sheep blood with addition of sodium azide (50 micrograms/ml), nalidixic acid (25 micrograms/ml), colistin (12.5 micrograms/ml) and crystal violet (2 micrograms/ml). The differential medium consisted of heart infusion agar and antiserum specific only for S. suis type 2. In a total of 291 pigs tested by a combination of these media, S. suis type 2 was isolated and confirmed from 40 of them (13.7%).  相似文献   

Narrow-band ultraviolet B therapy attenuates cutaneous T-cell responses in hapten-induced,experimental contact dermatitis in beagles     

Saki Onishi-Sakamoto  Kiichi Makishi  Kazumoto Takami  Ryota Asahina  Sadatoshi Maeda  Masahiko Nagata  Peter F. Moore  Kaori Ide  Koji Nishifuji 《Veterinary dermatology》2021,32(6):605-e161

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