Relationship of the bovine growth hormone gene to carcass traits in Japanese black cattle.   总被引：1，自引：0，他引：1  

K Tatsuda  A Oka  E Iwamoto  Y Kuroda  H Takeshita  H Kataoka  S Kouno 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2008,125(1):45-49

The bovine growth hormone gene (bGH) possesses three haplotypes, A, B and C, that differ by amino acid mutations at positions 127 and 172 in the fifth exon: (leucine 127, threonine 172), (valine 127, threonine 172) and (valine 127, methionine 172) respectively. The correlation between meat quality or carcass weight and these haplotypes was investigated in Japanese black cattle. Altogether, 940 bGH haplotypes were compared with respect to six carcass traits: carcass weight, longissimus muscle area, rib thickness, subcutaneous fat thickness, beef marbling score and beef colour. The frequency of the B haplotype was higher (0.421) than that of A (0.269) and C (0.311). High carcass weight and low beef marbling were associated with haplotype A (p < 0.05 and p < 0.01 respectively), whereas beef marbling was increased by haplotype C (p < 0.05). Estimated regression coefficient of the A haplotype substitution effect for carcass weight and beef marbling score were 5.55 (13.1% of the phenotypic SD) and -0.31 (17.0%) respectively. That of the C haplotype for beef marbling score was 0.20 (11.0%). The other traits showed no relationship to the haplotypes examined. The results of this investigation suggest that information pertaining to bGH polymorphisms in Japanese black cattle could be used to improve the selection of meat traits.  相似文献   

Estimation of beef marbling in the Longissimus muscle with computer image analysis of ultrasonic pictures of the Iliocostalis muscle area     

Toshihiro Nade  Yuya Kataoka  Kazuhiro Hoshi  Ikuo Maeda  Kunihiko Saito 《Animal Science Journal》2014,85(3):247-253

This study investigated an objective method for estimating beef marbling using ultrasonic images of the Iliocostalis muscle and the Lomgissimus muscle area sections. Thirty‐one Japanese Black cattle steers were used in this study. The end of the left side shoulder blade bone was scanned using an ultrasonic device. Ultrasonic images were captured of the Longissimus muscle area and that around the Iliocostalis muscle area. Twenty items were measured in the two images using computer image analysis software. The level of beef marbling was measured according to the Beef Marbling Standard (BMS) for carcass grading, and the percentage of ether‐extractable fat content in the Longissimus muscle (EE). The difference in the gray level between the Iliocostalis muscle and intermuscular fat (X10) was used to estimate the BMS and the EE, which were highly correlated (r² = 67.72% and 61.30%). An equation was developed using four parameters from the two ultrasonic images, which could estimate the BMS (r² = 85.88%). This equation could also estimate the EE (r² = 68.98%). The equations used to estimate beef marbling were based on one to four parameters that included X10. Thus, ultrasonic images of the Iliocostalis muscle area section are important for estimating beef marbling.  相似文献   

Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex     

Kim VN  Kataoka N  Dreyfuss G 《Science (New York, N.Y.)》2001,293(5536):1832-1836

Nonsense-mediated messenger RNA (mRNA) decay, or NMD, is a critical process of selective degradation of mRNAs that contain premature stop codons. NMD depends on both pre-mRNA splicing and translation, and it requires recognition of the position of stop codons relative to exon-exon junctions. A key factor in NMD is hUpf3, a mostly nuclear protein that shuttles between the nucleus and cytoplasm and interacts specifically with spliced mRNAs. We found that hUpf3 interacts with Y14, a component of post-splicing mRNA-protein (mRNP) complexes, and that hUpf3 is enriched in Y14-containing mRNP complexes. The mRNA export factors Aly/REF and TAP are also associated with nuclear hUpf3, indicating that hUpf3 is in mRNP complexes that are poised for nuclear export. Like Y14 and Aly/REF, hUpf3 binds to spliced mRNAs specifically ( approximately 20 nucleotides) upstream of exon-exon junctions. The splicing-dependent binding of hUpf3 to mRNAs before export, as part of the complex that assembles near exon-exon junctions, allows it to serve as a link between splicing and NMD in the cytoplasm.  相似文献   

Availability of black soldier fly meal as an alternative protein source to fish meal in red sea bream (Pagrus major,Temminck & Schlegel) fingerling diets     

Fumiaki Takakuwa  Ryota Tanabe  Shohei Nomura  Takashi Inui  Shinichi Yamada  Amal Biswas  Hideki Tanaka 《Aquaculture Research》2022,53(1):36-49

Black soldier fly meal (BM) is an outstanding candidate as a fish meal (FM) substitute because it contains relatively high protein and essential amino acids. In this study, we replaced FM in red sea bream diets (Pagrus major) with BM to investigate its effect on growth and feed utilization. Six isonitrogenous and isolipidic experimental diets were prepared by substituting 0%, 20%, 40%, 60%, 80% and 100% FM protein with BM (Control, BM20, BM40, BM60, BM80 and BM100, respectively). After the 8-week feeding trial, final body weight, weight gain, specific growth rate and feed efficiency decreased linearly with increasing dietary BM level (p < 0.05). Apparent digestibility of protein and fat and serum total cholesterol concentration decreased with increasing dietary BM levels (p < 0.05) Final body weight, WG, SGR and FE against dietary BM level had breaking points of 76.2%, 41.7%, 76.5% and 60.0%, respectively, in segmental regression analysis. In conclusion, the results suggest that BM can replace a maximum of 41.7% of FM in the diet of red sea bream without compromising growth performance or feed efficiency for 56 days.  相似文献   

Determination of S-methyl-, S-propyl-, and S-propenyl-L-cysteine sulfoxides by gas chromatography-mass spectrometry after tert-butyldimethylsilylation     

Tsuge K  Kataoka M  Seto Y 《Journal of agricultural and food chemistry》2002,50(16):4445-4451

A gas chromatographic-mass spectrometric method for the determination of S-methyl-L-cysteine sulfoxide (1), S-propyl-L-cysteine sulfoxide (2), and S-propenyl-L-cysteine sulfoxide (3), specific marker compounds in the genus Allium, is described. The target amino acids were converted to the tert-butyldimethylsilyl derivatives. The products were silylated on the amino and carboxyl groups and on an additional oxygen atom and were separated on a nonpolar capillary column. That incorporation of three tert-butyldimethylsilyl groups had occurred was verified by mass spectrometry, which gave an m/z 302 fragment as base peak (amino acid side chain eliminated ion) and m/z 436 (1), 464 (2), or 462 (3) as major peaks (tert-butyl function eliminated ion), by electron impact ionization. The detection limits for 1 and 2 under selected ion monitoring at m/z 436 (1) and m/z 464 (2), respectively, were determined to be 0.3 and 1.8 ng per injection. To clean up the analytes from the solvent extract of onion, as a representative food material, onion, the sample solution was subjected to combined solid phase extraction. The eluate from a Sep-Pak C(18) cartridge was applied to a Bond Elut SCX cartridge (H(+) form), followed by washing with 0.1 M hydrochloric acid and elution with 0.5 M ammonia. From a simulated matrix solution containing 5% sucrose, 1 and 2 were extracted quantitatively, and the detection yield was approximately 75%. The contents of 1, 2, and 3 in commercial onion were estimated to be 0.3, 3.1, and 3.0 mg, respectively, per gram of fresh weight.  相似文献   

Narrow-band ultraviolet B therapy attenuates cutaneous T-cell responses in hapten-induced,experimental contact dermatitis in beagles     

Saki Onishi-Sakamoto  Kiichi Makishi  Kazumoto Takami  Ryota Asahina  Sadatoshi Maeda  Masahiko Nagata  Peter F. Moore  Kaori Ide  Koji Nishifuji 《Veterinary dermatology》2021,32(6):605-e161

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Relationship between wettability and sizing degree of paper containing bulking agent     

Hailan Jin  Takayuki Okayama  Ryota Arai  Hajime Ohtani 《Journal of Wood Science》2011,57(1):34-39

The addition of a bulking agent achieves the bulking of paper and increases the pore volume. In this study, the effect of adding bulking agents with various alkyl chain lengths (C₁₄, C₁₈, and C₂₂) on the bulk of paper, the sizing performance, and sheet wettability was evaluated. The bulking effect of a bulking agent with a short alkyl chain length (C₁₄) was large, and reduction in the sheet tensile strength was confirmed to be suppressed. Good sheet sizing performance was achieved when a bulking agent was used with an alkyl ketene dimer (AKD). When 0.1% or more of AKD was added, using a bulking agent with a shorter alkyl chain length increased the improvement in the sizing degree. Pyrolysis gas chromatography measurements of the amount of reacted AKD revealed that the addition of a bulking agent increased the AKD content of the sheet. The sheet contact angle measured by the two-liquid method tended to increase with increasing alkyl chain length. However, the sheet contact angle was not directly correlated with the Stöckigt sizing degree. By using 1% ferric chloride (FeCl₃) and 2% ammonium thiocyanate (NH₄SCN) aqueous solution, which are used for measuring the Stöckigt sizing degree, the time-dependent change in the contact angle on the paper sheet surface was measured to evaluate wettability. The Stöckigt sizing degree of paper sheets was found to be greatly influenced by the sheet wettability by NH₄SCN solution. In the case of 2% NH₄SCN aqueous solution, different samples exhibited large wettability differences. When 0.2% or less of AKD was added, the effect of the concentration on the contact angle was confirmed to be large at all NH₄SCN concentrations.  相似文献   

Spatial structures of N₂O, CO₂, and CH₄ fluxes from Acacia mangium plantation soils during a relatively dry season in Indonesia     

Ryota Konda  Seiichi Ohta  Shigehiro Ishizuka  Seiko Arai  Saifuddin Ansori  Nagaharu Tanaka  Arisman Hardjono 《Soil biology & biochemistry》2008,40(12):3021-3030

We investigated spatial structures of N₂O, CO₂, and CH₄ fluxes during a relatively dry season in an Acacia mangium plantation stand in Sumatra, Indonesia. The fluxes and soil properties were measured at 1-m intervals in a 1 × 30-m plot (62 grid points) and at 10-m intervals in a 40 × 100-m plot (55 grid points) at different topographical positions of the upper plateau, slope, and valley bottom in the plantation. Spatial structures of each gas flux and soil property were identified using geostatistical analysis. The means (±SD) of N₂O, CO₂, and CH₄ fluxes in the 10-m grids were 0.54 (±0.33) mg N m⁻² d⁻¹, 2.81 (±0.71) g C m⁻² d⁻¹, and −0.84 (±0.33) mg C m⁻² d⁻¹, respectively. This suggests that A. mangium soils function as a larger source of N₂O than natural forest soils in the adjacent province on Sumatra during the relatively dry season, while CO₂ and CH₄ emissions from the A. mangium soils were less than or consistent with those in the natural forest soils. Multiple spatial dependence of N₂O fluxes within 3.2 m (1-m grids) and 35.0 m (10-m grids), and CO₂ fluxes within 1.8 m (1-m grids) and over 65 m (10-m grids) was detected. From the relationship among N₂O and CO₂ gas fluxes, soil properties, and topographic elements, we suggest that the multiple spatial structures of N₂O and CO₂ fluxes are mainly associated with soil resources such as readily mineralizable carbon and nitrogen in a relatively dry season. The soil resource distributions were probably controlled by the meso- and microtopography. Meanwhile, CH₄ fluxes were spatially independent in the A. mangium soils, and the water-filled pore space appeared to mainly control the spatial distribution of these fluxes.  相似文献   

Absorption and translocation of nitrogen in cucumber (Cucumis sativus L.) plants using the ¹⁵N tracer technique     

Ryota TANEMURA  Hiroshi KURASHIMA  Norikuni OHTAKE  Kuni SUEYOSHI  Takuji OHYAMA 《Soil Science and Plant Nutrition》2008,54(1):108-117

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In situ observation of pinewood nematode in wood     

Toshihiro Umebayashi  Toshihiro Yamada  Kazunari Fukuhara  Ryota Endo  Dai Kusumoto  Kenji Fukuda 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(2):463-467

In pine wilt disease, xylem dysfunction occurs in relation to nematode migration and proliferation in host tissue, but the detection accuracy of pinewood nematode (PWN), Bursaphelenchus xylophilus, in pine stem tissue remains unclear. This study describes the use of cryo-scanning electron microscopy (cryo-SEM) and fluorescein-conjugated wheat germ agglutinin (F-WGA) staining to detect PWN. After PWN invasion, the frequency of surface fluorescence in PWN increased in pine stems from the day of inoculation to 3 weeks after inoculation. However, the fluorescence frequency decreased significantly during the advanced disease stage after 5 weeks. Thus, detecting PWN based on fluorescent staining of the nematode body surface coat protein can be misleading when used to examine the correlation between the development of disease symptoms and the nematode population. In contrast, all cut body segments were fluorescent, and their fluorescent components were common in pine-stem cross sections, regardless of the timing after inoculation. In addition, PWN were observed under cryo-SEM only in empty resin canals and this distribution was confirmed by F-WGA staining of PWN cut in a cross section. Thus, PWN detection based on fluorescent staining of surface coat proteins by F-WGA was not reliable in longitudinal sections of pine stems because of changes in nematode stainability during disease progression. To detect PWN in infected plants, we concluded that a combination of both methods is most effective.  相似文献