Gating Behavior of Endoplasmic Reticulum Potassium Channels of Rat Hepatocytes in Diabetes     

Maedeh Ghasemi  Naser Khodaei  Sajjad Salari  Afsaneh Eliassi  Reza Saghiri 《Iranian Biomedical Journal》2014,18(3):165-172

Background: Defects in endoplasmic reticulum homeostasis are common occurrences in different diseases, such as diabetes, in which the function of endoplasmic reticulum is disrupted. It is now well established that ion channels of endoplasmic reticulum membrane have a critical role in endoplasmic reticulum luminal homeostasis. Our previous studies showed the presence of an ATP-sensitive cationic channel in endoplasmic reticulum. Therefore, in this study, we examined and compared the activities of this channel in control and diabetic rats using single-channel recording techniques. Method: Male Wistar rats were made diabetic for 2 weeks with a single dose injection of streptozotocin (45 mg/kg). Ion channel incorporation of rough endoplasmic reticulum of diabetic hepatocytes into the bilayer lipid membrane allowed the characterization of K⁺ channel. Results: Ion channel incorporation of rough endoplasmic reticulum vesicles into the bilayer lipid revealed that the channel current-voltage (I-V) relation with a mean slope conductance of 520 ± 19 pS was unaffected in diabetes. Interestingly, the channel Po-voltage relation was significantly lower in diabetic rats at voltages above +30 mV. Conclusion: We concluded that the endoplasmic reticulum cationic channel is involved in diabetes. Also, this finding could be considered as a goal for further therapeutic plans. Key Words: Endoplasmic reticulum, Diabetes, Ion channels, Bilayer lipid membrane, Liver  相似文献   

Physico-Chemical Profiling of Promising Sweet Orange Cultivars Grown Under Different Agro-Climatic Conditions of Pakistan     

Syed Bilal Hussain  Muhammad Akbar Anjum  Sajjad Hussain  Shaghef Ejaz  Maqsood Ahmed 《Erwerbs-Obstbau》2017,59(4):315-324

Sweet oranges are produced on large scale all over the world and demanded due to less number of seeds and are better juice quality. A study was carried out with the objective to characterize promising sweet orange cultivars for better quality fruits production under different agro-climatic conditions of Punjab (Pakistan). Fruits of three promising sweet orange (Citrus sinensis L. Osbeck) cultivars viz. ‘Blood Red’, ‘Musambi’ and were harvested from commercial citrus groves at five different locations. Starting from Multan, in the direction of increasing altitude, four more sites viz. Sahiwal, Faisalabad, Sargodha and Chakwal were selected after every 100–150?km distance. Fruits were evaluated in terms of different physical and biochemical characters. Results showed significant influence of climatic conditions on physico-chemical properties of the cultivars at all the locations. Fruits harvested from Multan and Sahiwal showed higher fruit weight, diameter and peel weight. More juicy fruits with higher vitamin C content were produced under mild temperature environmental conditions of Chakwal. Overall, ‘Musambi’ fruits had more total soluble solids and reducing and total sugars. ‘Musambi’ was found to be good source of bioactive compounds i.?e. vitamin C and total phenolic content, had the maximum antioxidant activity as compared to ‘Blood Red’ and ‘Succari’ and may be explored as potential cultivar in Sargodha and Chakwal.  相似文献   

Biodiversity in Indigenous Germplasm of <Emphasis Type="Italic">Pyrus </Emphasis>from Pakistan Based on Phenotypical and Morphological Traits     

Maqsood Ahmed  Muhammad Akbar Anjum  Sajjad Hussain  Shaghef Ejaz  Shakeel Ahmad  Sezai Ercisli 《Erwerbs-Obstbau》2017,59(1):19-27

A comprehensive analysis of various horticulturally important morphological traits of sixty pear accessions located in five districts of Azad Jammu and Kashmir (Pakistan) was carried out. Several qualitative and quantitative traits were investigated in situ and after the harvesting of leaf and fruit samples. The rich diversity was observed for qualitative traits (i.?e. growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, and fruit shape and color) among pear accessions. As for the quantitative traits, significant variation was observed for leaf area (8.96–27.17 cm²), flowering duration (11–24 days), fruit weight (68.1–322.3 g), average fruit length (2.68–9.87 cm) and average fruit width (1.82–8.12 cm) between pear accessions. Based on qualitative and quantitative data, two dendograms were constructed by using cluster analysis which separated pear accessions into three distinct groups. The cluster analysis showed considerable differences between the accessions for qualitative and quantitative traits. Furthermore, a close association between accessions was noted inside the clusters for qualitative and quantitative traits. The results showed rich diversity and similarity between the pear accessions and needs further assessment at molecular level to elucidate their phylogenetic relationship.  相似文献   

Zinc and Boron Application at Different Phenological Stages Alleviates Tree Growth,Fruit Yield and Quality of Sweet Orange Cv. ‘Blood Red’     

Walli  Sammen  Hafiz  Ishfaq Ahmed  Khan  Rashid Iqbal  Bashir  Muhammad Ajmal  Uddin  Sareer  Khan  Shakir Ullah  Ajmal  Usman  Hussain  Sajjad  Khalid  Muhammad Fasih 《Gesunde Pflanzen》2022,74(2):385-396

The deficiency of micronutrients, particularly zinc (Zn) and boron (B) has affected vegetative growth, yield and fruit quality of sweet orange in many citrus growing areas of Pakistan. The present study was conducted in 2015 and 2016 to determine the impact of Zn and B supplementation on growth and quality of sweet orange cv. ‘Blood red’. Pre-harvest foliar application of variant Zn and B levels was done at different phenological stages of fruit growth and development, i.e., full bloom, fruit set and premature stage. Results have concluded that pre-harvest foliar application of Zn and B augmented phosphorous, Zn, B, potassium, iron, and manganese contents in leaves, particularly Zn and B content from deficient to optimal level. Moreover, the combined application of Zn and B has also significantly enhanced vegetative characters such as tree height and spread along with reproductive characters, i.e., incremented numbers of fruit, fruit weight, total soluble solids, titratable acidity and ascorbic acid contents. However, among stage comparison, outcomes depicted that foliar application of Zn and B at full bloom?+?fruit set?+?premature stage resulted in maximum production with high quality fruits.

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Genetic diversity and population structure assessed through simple sequence repeat markers in Saccharum spp. hybrids from Pakistan and Sri Lanka     

Shahzad  Sundas  Saeed  Muhammad  Perveen  Shagufta  Azeem  Muhammad  Sajjad  Iqra  Saeed  Shazia  Naeem  Muhammad  Javed  Alia  Sultan  Iqra  Faria  Ukasha  Maryam  Sana  Yousaf  Muhammad Zaid 《Genetic Resources and Crop Evolution》2022,69(8):2889-2900

Genetic Resources and Crop Evolution - The Saccharum species members are extremely complex allopolyploids. In this study, sixty Saccharum spp. hybrids were analyzed using simple sequence repeat...  相似文献   

Potential of rooftop rainwater harvesting to meet outdoor water demand in arid regions     

Kazi TAMADDUN  Ajay KALRA  Sajjad AHMAD 《干旱区科学》2018,10(1):68-83

The feasibility of rooftop rainwater harvesting(RRWH) as an alternative source of water to meet the outdoor water demand in nine states of the U.S. was evaluated using a system dynamics model developed in Systems Thinking, Experimental Learning Laboratory with Animation. The state of Arizona was selected to evaluate the effects of the selected model parameters on the efficacy of RRWH since among the nine states the arid region of Arizona showed the least potential of meeting the outdoor water demand with rain harvested water. The analyses were conducted on a monthly basis across a 10-year projected period from 2015 to 2024. The results showed that RRWH as a potential source of water was highly sensitive to certain model parameters such as the outdoor water demand, the use of desert landscaping, and the percentage of existing houses with RRWH. A significant difference(as high as 37.5%) in rainwater potential was observed between the projected wet and dry climate conditions in Arizona. The analysis of the dynamics of the storage tanks suggested that a 1.0–2.0 m~3 rainwater barrel, on an average, can store approximately 80% of the monthly rainwater generated from the rooftops in Arizona, even across the high seasonal variation. This interactive model can be used as a quick estimator of the amount of water that could be generated, stored, and utilized through RRWH systems in the U.S. under different climate conditions. The findings of such comprehensive analyses may help regional policymakers, especially in arid regions, to develop a sustainable water management infrastructure.  相似文献   

Assessing the drivers of land use change in the Rumpi hills forest protected area,Cameroon     

Mukete Beckline  Daniel Etongo  Sajjad Saeed  Abdul Mannan 《Journal of Sustainable Forestry》2018,37(6):592-618

Protected areas serve two objectives, biodiversity conservation and securing of ecosystem services. But the recent expansion of human activities around Cameroon’s Rumpi Hills Forest Reserve in this Era of Sustainable Development Goals is a call for concern. This study assesses land use and land cover change around the reserve and their potential impact on its sustainability. A total of 250 household surveys were conducted across 13 villages in four selected sites around the reserve in addition to 11 focus group discussions. Landsat 7 ETM (2000) and Landsat 8 OLI (2014) images and topographic maps were utilized to quantify land use and land cover change.

During the 14-year period, dense forest dropped to 90.2% while settlements increased from 744.6 to 2148.8 hectares in 2014. Also, farmlands increased by 18.25% representing a change from 9400.4 to 11117.16 hectares. Over 98% and 85% of the respondents are engaged in the cultivation of food and cash crops and are dependent on the forests for timber and non-timber forests products. Land and tree-based interventions that improve soil fertility should be promoted among smallholder farmers to ensure the sustainability of protected areas in Cameroon.  相似文献   

Amelioration of cadmium stress in gladiolus (Gladiolus grandiflora L.) by application of potassium and silicon     

Malik Muhammad Zaheer  Sajid Rashid Ahmad  Waheed Ullah Khan  Aqeel Ahmad  Aamir Ali 《Journal of plant nutrition》2018,41(4):461-476

Gladiolus corms were grown in media contaminated with cadmium (Cd) (50 mg kg^?1) and supplemented with silicon (Si) and potassium (K). The role of Si and K for mitigation of Cd toxicity was evaluated. Cd-induced stress generated significantly increased level of oxidative stress markers including hydrogen peroxide (H₂O₂), and malondialdehyde (MDA) in gladiolus. The application of K and Si improved the production of protein and proline in the treated plants. Moreover, K and Si supplemented plants exhibited an improvement in the activity of antioxidant enzymes and a reduction in the level of MDA, H₂O₂ and Cd uptake under Cd stress. Application of K and Si also enhanced the uptake of mineral nutrients including calcium (Ca), magnesium (Mg), manganese (Mn), sulfur (S) and K. The plants supplemented with K and Si exhibited a higher amount of total phenolics and flavonoids. The combined effect of Si and K was more pronounced regarding beneficial effects on gladiolus plants compared to individual effect of these elements under Cd stress. The current research reveals that Si and K may improve gladiolus growth by decreasing the oxidative stress and Cd uptake and by increasing the activity of antioxidant defense enzymes, the quantity of secondary metabolites and plant nutrition.  相似文献   

Dynamics of innate immune response in Gallus domesticus using two methods of induced molting     

Sandhu MA  Rahman ZU  Rahman SU  Hassan IJ 《Veterinary immunology and immunopathology》2007,120(3-4):106-114

The aim of this study was to compare the ability of laying hen abdominal macrophages during the second production cycle by using two different methods of induced molting. Two groups of Single Comb White Leghorn hens were induced to molt at the end of their first production cycle using feed restriction and ZnO supplementation. Macrophages were isolated from the abdomen and in vitro cytotoxic ability, at which point macrophage bactericidal moiety nitric oxide (NO) was recorded. Serum IgM and IgG titers against sheep red blood cells (SRBC) were determined at various stages: before molting (BM), 5% production level (5P), peak production stage (PP) and at the end of production (EP) level after fast and Zn-induced molt. Macrophages adherence percentage remained unaffected (p ≤ 0.05) during all production cycles, whereas the macrophage engulfment percentage and engulfment/cell was significantly higher (p ≤ 0.05) at PP in both fast and Zn-induced molted groups, as compared to all other studied stages. Macrophage NO production was increased (p ≤ 0.05) at PP and after SRBC and lipopolysaccrides (LPS) stimulus, when molted with ZnO supplementation. Serum total antibody titer against SRBC increased serum IgG and IgM titers during the second production cycle by Zn-induced molt. However, molting stress greatly reduced IgG and IgM production at the 5P stage. Serum Zn concentration increased with the onset of production but decreased at the EP stage irrespective of their molting regimes. Our results validate the strengthened innate and acquired immune response during the second production cycle after Zn-induced molting instead of fasting.  相似文献   

An improved method with a wider applicability to isolate plant mitochondria for mtDNA extraction     

Zaheer?Ahmed  Yong-Bi?FuEmail author 《Plant methods》2015,11(1):56

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