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Conventional methods have been used to study the general configuration of the wall of the Sinus paranalis in dogs. Apocrine tubular glands, elastic fibres and smooth-muscle fibres are the more significant elements of the wall, together with the epithelium and connective tissue. By means of the immunohistochemical method described in this paper, the results provide histochemical evidence for the presence of an AChE-positive reaction, and supposedly cholinergic nerve fibres in the wall of the dog anal sacs, mainly associated with subepithelial smooth muscle, vessels, and glands.  相似文献   
123.
In the strawberry crop area of Tucumán (north-west Argentina) the three species of Colletotrichum causing anthracnose disease (C. acutatum, C. fragariae and C. gloeosporioides) were detected. Among all isolates characterized, one of them identified as C. acutatum (M11) and another as C. fragariae (F7) were selected due to their conspicuous interaction with the strawberry cultivar Pájaro. Whereas isolate M11 produced a strong compatible interaction in cv. Pájaro with clear disease symptoms (DSR = 5.0), the isolate F7 brought about a typical incompatible interaction (DSR = 1.0). When plants of cv. Pájaro were inoculated with F7 prior to the inoculation with M11, the former avirulent strain prevented the growth of the latter virulent pathogen. Experimental evidence indicated that the time elapsed between the first inoculation with the avirulent pathogen and the second inoculation with the virulent one was crucial to inhibit the growth of the latter. The growth of F7 on the plant without provoking damage and the fact that there was no in vitro antagonistic effect between the pathogens, suggests that the avirulent strain triggers a plant defensive response against M11. The defense response was further confirmed by the detection of an early oxidative burst occurring within 4 h after the first inoculation and by the observation of anatomical changes associated with defense mechanisms that lasted 50 days after the inoculation with F7. Results obtained support the hypothesis that the plant resistance against the virulent strain M11 is elicited by one or more diffusible(s) compound(s) produced by the avirulent strain F7.  相似文献   
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After successive vegetative propagation cycles, strawberry (Fragaria × ananassa Duch.) plants often accumulate multiple virus species that result in viral symptoms and losses in yield and quality. However, strawberry plants infected by a single virus species usually remain asymptomatic with unknown effects on fruit production and quality. In this context, the effect of Strawberry mild yellow edge virus (SMYEV) on fruit production was studied in strawberry plants, cultivar Camarosa, over two years. Asymptomatic SMYEV-infected plants showed a significant reduction in total and marketable fruit number and weight compared with healthy plants. These reductions ranged between 28% and 63%, depending on the parameter measured and the production cycle. Fluctuations in SMYEV concentration in the plants was detected throughout the crop cycle, suggesting that samples for virus diagnosis should be taken when the plant has the highest virus concentration; in this study, this occurred at the end of the crop cycle. These results show that analyzing symptomless strawberry plants should be part of a virus disease management plant and an important component to control the quantitative and qualitative impacts of SMYEV on strawberry yield.  相似文献   
126.
Hispanic farm workers and their families in the U.S. face a number of environmental and occupational health risks, yet they are rarely given the opportunity to choose the focus of the research and interventions that take place in their communities. Community-based participatory research (CBPR) can be one effective approach to changing this situation. CBPR is an approach to research that makes community members partners in research rather than subjects of research. This article describes the experience of El Proyecto Bienestar (The Well-Being Project), a CBPR project in the Yakima Valley, Washington, with the aim of facilitating the Hispanic community's involvement in the identification and prioritization of occupational and environmental health issues among farm workers. The project utilized three forms of data collection (key informant interviews, community surveys, and a town hall meeting) to create a list of environmental and occupational health issues of concern. Investigators strove to provide opportunities for community participation in the various stages of research: study concept and design, data collection, data analysis and interpretation, conclusions, and dissemination of results. This article describes the involvement that community members had at each stage of the three forms of data collection and outlines the basic findings that led the Community Advisory Board to prioritize four areas for future work. In addition, it describes the challenges the project faced from the researcher perspective. Using examples from this experience, we conclude that this model may be an effective way for farm workers and their families to have a voice in prioritizing health and safety issues for research and action in their communities.  相似文献   
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The objective of this study was to evaluate the effect of two sources of commercial porcine pituitary‐derived follicle‐stimulating hormone (pFSH) and pFSH—porcine Luteinizing Hormone (pLH), including equine chorionic gonadotropin (eCG), in ovulatory and embryonic response in Pelibuey sheep. Twenty‐four Pelibuey sheep were used and were assigned randomly to four treatments (n = 6): (T1; 200 mg pFSH‐Folltropin®); (T2; 200 mg pFSH + 300 UI eCG‐Folligon®); (T3; 250 UI pFSH/pLH‐Pluset®) and (T4; 250 UI pFSH/pLH + 300 UI eCG). The interval of hours from withdrawal of the device to the beginning of oestrus (BO) was lower (p < .05) in sheep treated with eCG (T2 = 8.0 ± 1.4 and T4 = 10.0 ± 2.8) than in those without eCG (T1 = 12.6 ± 0.6 and T3 = 20.6 ± 2.4). The ovulatory rate (OR) was higher (p < .05) in T1 = 15.5 ± 2.8 and T2 = 15.6 ± 1.4, compared to T3 = 8.1 ± 3.2 and T4 = 11.8 ± 2.8; a significant difference was not shown between them (T1 vs. T2 and T3 vs. T4) when including eCG. The number of non‐fertilized oocytes (NFO) was lower (p ? .05) in T1 = 0.8 ± 0.4 and T3 = 1.8 ± 1.8, compared to those that included eCG (T2 = 6.3 ± 2.4 and T4 = 2.1 ± 1.2). The number of transferable embryos (TE) was higher (p < .05) when FSH was applied (T1 = 5.8 ± 1.1), compared with (T2 = 2.6 ± 1.1, T3 = 2.3 ± 1.4 and T4 = 2.8 ± 1.5). The commercial treatments (pFSH or pFSH‐pLH) in combination with eCG did not improve OR, NFO and TE. However, the exclusive pFSH (Folltropin) treatment presented a higher OR, lower number of NFO and higher number of TE.  相似文献   
129.
In Reserva Natural de Castro Marim e Vila Real de Santo António (SE of Portugal), most of the salt marshes and saltpans are abandoned contributing to their degradation and, consequently, alteration of some ecological conditions. Rehabilitation of these areas by a Salicornia crop can contribute to their economic and environmental improvement by stimulation of biogeochemical processes and biomass commercialization. However, the development of agro-techniques adjusted to species and variable environmental conditions from Mediterranean are needed in order to improve the Salicornia crop. This study aimed to evaluate: i) potential use of seedlings from greenhouse in the field cultivation; and ii) survival, growth and yield of Salicornia under shading and different salinity conditions of the soils and irrigation waters. Autochthonous Salicornia species (S. ramosissima and S. patula) were tested. Germination tests under controlled conditions and in situ mesocosm assays were carried out. Assays were realized with a low density of seedlings from natural conditions and greenhouse, and soils and waters from estuary both with different salinities. Intermediate and high salinities (25–45?dS/m) affected only S. patula germination. In general, Salicornia growing in soils with low salinity had great fresh biomass accumulation independently of seedling source (from natural conditions and greenhouse) and/or growing season. The shading seems to improve the fresh biomass accumulation and yield. Under Mediterranean conditions, the transplantation of Salicornia from greenhouse to the field was a promising technique, independently of the salinity conditions. This study demonstrated that the abandoned and saline areas can be rehabilitated by a sustainable crop of Salicornia.  相似文献   
130.
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