Mitochondrial DNA Copy Number Variations and Serum Pepsinogen Levels for Risk Assessment in Gastric Cancer     

Mehdi Alikhani  Samaneh Saberi  Maryam Esmaeili  Valrie Michel  Mohammad Tashakoripour  Afshin Abdirad  Arezoo Aghakhani  Sana Eybpoosh  Massoud Vosough  Mohammad Ali Mohagheghi  Mahmoud Eshagh Hosseini  Eliette Touati  Marjan Mohammadi 《Iranian Biomedical Journal》2021,25(5):323

Background:Variations in mtDNA-CN of PBLs, as a potential biomarker for GC screening has currently been subject to controversy. Herein, we have assessed its efficiency in GC screening, in parallel and in combination with sPG I/II ratio, as an established indicator of gastric atrophy. Methods:The study population included GC (n = 53) and non-GC (n = 207) dyspeptic patients. The non-GC group was histologically categorized into CG (n = 104) and NM (n = 103) subgroups. The MtDNA-CN of PBLs was measured by quantitative real-time PCR. The sPG I and II levels and anti-H. pylori serum IgG were measured by ELISA. Results:The mtDNA-CN was found significantly higher in GC vs. non-GC (OR = 3.0; 95% CI = 1.4, 6.4) subjects. Conversely, GC patients had significantly lower sPG I/II ratio than the non-GC (OR = 3.2; CI = 1.4, 7.2) subjects. The combination of these two biomarkers yielded a dramatic amplification of the odds of GC risk in double-positive (high mtDNA-CN-low sPGI/II) subjects, in reference to double-negatives (low mtDNA-CN-high sPGI/II), when assessed against non-GC (OR = 27.1; CI = 5.0, 147.3), CG (OR = 13.1; CI = 2.4, 72.6), or NM (OR = 49.5; CI = 7.9, 311.6) groups. Conclusion:The combination of these two biomarkers, namely mtDNA-CN in PBLs and serum PG I/II ratio, drastically enhanced the efficiency of GC risk assessment, which calls for further validations. Key Words: Biomarkers, DNA copy number variation, Mitochondrial DNA, Stomach neoplasms  相似文献   

Sustainable futures over the next decade are rooted in soil science     

Daniel L. Evans  Victoria Janes-Bassett  Pasquale Borrelli  Claire Chenu  Carla S. S. Ferreira  Robert I. Griffiths  Zahra Kalantari  Saskia Keesstra  Rattan Lal  Panos Panagos  David A. Robinson  Samaneh Seifollahi-Aghmiuni  Pete Smith  Tammo S. Steenhuis  Amy Thomas  Saskia M. Visser 《European Journal of Soil Science》2022,73(1):e13145

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