全文获取类型
收费全文 | 1586篇 |
免费 | 54篇 |
专业分类
林业 | 35篇 |
农学 | 38篇 |
基础科学 | 1篇 |
82篇 | |
综合类 | 901篇 |
农作物 | 22篇 |
水产渔业 | 45篇 |
畜牧兽医 | 445篇 |
园艺 | 7篇 |
植物保护 | 64篇 |
出版年
2023年 | 9篇 |
2022年 | 8篇 |
2021年 | 22篇 |
2020年 | 22篇 |
2019年 | 29篇 |
2018年 | 25篇 |
2017年 | 17篇 |
2016年 | 14篇 |
2015年 | 16篇 |
2014年 | 13篇 |
2013年 | 59篇 |
2012年 | 31篇 |
2011年 | 47篇 |
2010年 | 25篇 |
2009年 | 23篇 |
2008年 | 41篇 |
2007年 | 37篇 |
2006年 | 47篇 |
2005年 | 42篇 |
2004年 | 32篇 |
2003年 | 39篇 |
2002年 | 45篇 |
2001年 | 60篇 |
2000年 | 59篇 |
1999年 | 36篇 |
1998年 | 12篇 |
1996年 | 6篇 |
1995年 | 12篇 |
1994年 | 11篇 |
1993年 | 31篇 |
1992年 | 34篇 |
1991年 | 38篇 |
1990年 | 38篇 |
1989年 | 52篇 |
1988年 | 46篇 |
1987年 | 48篇 |
1986年 | 53篇 |
1985年 | 32篇 |
1984年 | 40篇 |
1983年 | 53篇 |
1982年 | 55篇 |
1981年 | 65篇 |
1980年 | 43篇 |
1979年 | 62篇 |
1978年 | 10篇 |
1977年 | 7篇 |
1974年 | 9篇 |
1973年 | 12篇 |
1971年 | 11篇 |
1966年 | 4篇 |
排序方式: 共有1640条查询结果,搜索用时 15 毫秒
81.
82.
Neomycin toxicosis in calves 总被引:1,自引:0,他引:1
W A Crowell T J Divers T D Byars A E Marshall K E Nusbaum L Larsen 《American journal of veterinary research》1981,42(1):29-34
Calves (n = 4) were given neomycin (2.25 or 4.5 mg/kg) twice daily IM and were compared with 2 calves given penicillin IM. The 2 hallmarks of aminoglycoside toxicosis, nephrotoxicosis and ototoxicosis, were seen with both dosages of parenterally administered neomycin. Nephrotoxicosis was confirmed by abnormal findings in urinalysis (granular casts, proteinuria, low specific gravity), renal biopsy results (tubular degeneration and necrosis), and increased 24-hour amounts of urinary enzymes (alanine aminopeptidase and gamma-glutamyltranspeptidase). Azotemia, decreased creatinine clearance, polyuria, and polydipsia also were documented in calves given neomycin. Clinically, deafness was suspected in 2 calves and was documented by electrical auditory-evoked response tests. Abnormalities in partial thromboplastin times and renal residues of neomycin were seen in all 4 calves that were given neomycin, but not in calves that were given penicillin. 相似文献
83.
Marshall E 《Science (New York, N.Y.)》2002,296(5569):824-825
84.
85.
Kittelberger R Reichel MP Jenner J Heath DD Lightowlers MW Moro P Ibrahem MM Craig PS O'Keefe JS 《Veterinary parasitology》2002,110(1-2):57-76
The aim of this study was to develop an immunological method for the identification of sheep infected with Echinococcus granulosus which would allow the monitoring of animals imported into countries free from hydatidosis and as an aid to countries where control schemes for the disease are in operation. Three enzyme-linked immunosorbent assays (ELISAs) were developed and validated, using as antigen either a purified 8 kDa hydatid cyst fluid protein (8kDaELISA), a recombinant EG95 oncosphere protein (OncELISA) or a crude protoscolex preparation (ProtELISA). Sera used for the assay validations were obtained from 249 sheep infected either naturally or experimentally with E. granulosus and from 1012 non-infected sheep. The highest diagnostic sensitivity was obtained using the ProtELISA at 62.7 and 51.4%, depending on the cut-off. Assay sensitivities were lower for the 8kDaELISA and the OncELISA. Diagnostic specificities were high, ranging from 95.8 to 99.5%, depending on the ELISA type and cut-off level chosen. A few sera from 39 sheep infected with T. hydatigena and from 19 sheep infected with T. ovis were recorded as positive. Western immunoblot analysis revealed that the dominant antigenic components in the crude protoscolex antigen preparation were macromolecules of about 70-150 kDa, most likely representing polysaccharides. This study demonstrated that the ProtELISA was the most effective immunological method of those assessed for detection of infection with E. granulosus in sheep. Because of its limited diagnostic sensitivity of about 50-60%, it should be useful for the detection of the presence of infected sheep on a flock basis and cannot be used for reliable identification of individual animals infected with E. granulosus. 相似文献
86.
Jankowski MK Ogilvie GK Lana SE Fettman MJ Hansen RA Powers BE Mitchener KM Lovett SD Richardson KL Parsley L Walton JA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(1):105-108
Matrix metalloproteinases (MMPs) are enzymes that play key roles in angiogenesis, tumor invasion, and metastasis in a wide variety of species. The purpose of this study was to evaluate pro and active MMP 2 and 9 concentrations in tumor, normal stromal tissue, and serum from tumor-bearing cats. We hypothesized that serum concentrations of pro and active forms of MMPs 2 and 9 would be predictive of MMP concentrations in tumor tissue and that these MMP concentrations would correlate with the histopathologic grade of the malignancies. Pro and active forms of MMPs 2 and 9 were determined by gelatin zymography and subsequent computerized densitometry from tumor and nearby stromal tissue and serum from 49 cats with various malignancies. The serum concentrations of MMPs from these tumor-bearing cats were compared with serum concentrations of MMPs from 44 normal cats of similar age and gender. Measurable concentrations of MMPs 2 and 9 were found within tumor, stromal, and serum samples. Mean concentrations of total pro and active MMPs 2 and 9 within tumor tissue were significantly higher (P values <.0001, .0031, <.001, and .0064, respectively) when compared with stromal tissue from the same animals. Serum MMP concentrations from tumor-bearing cats were higher than those from normal cats. Poor correlation was found between serum MMP concentrations and tissue MMP concentrations of increasing histologic grades of malignancies. 相似文献
87.
88.
Marshall E 《Science (New York, N.Y.)》2000,287(5453):565, 567
89.
90.