Potential of three new krill products for seabream larval production     

Mónica Beatriz Betancor  Sigve Nordrum  Eyad Atalah  María José Caballero  Tibiábin Benítez‐Santana  Javier Roo  Lidia Robaina  Marisol Izquierdo 《Aquaculture Research》2012,43(3):395-406

The objective of this study was to evaluate three products derived from krill as sources of essential fatty acids, protein and, particularly, phospholipids in microdiets for larval gilthead seabream (Sparus aurata). Their effect on larval performance, biochemical composition and histological development was investigated. The addition of krill phospholipids, rich in highly unsaturated fatty acids, improved larval sea bream growth in terms of weight and length, enhanced hepatic utilization of dietary lipids and reduced the incidence of enterocyte injuries. These results confirm the higher nutritional value of marine phospholipids for the early development of marine fish larvae in comparison with soybean phospholipids.  相似文献   

Biomarkers of bone development and oxidative stress in gilthead sea bream larvae fed microdiets with several levels of polar lipids and α‐tocopherol          下载免费PDF全文

R. Saleh  M.B. Betancor  J. Roo  T. Benítez‐Santana  M.J. Zamorano  M. Izquierdo 《Aquaculture Nutrition》2015,21(3):341-354

Although dietary marine phospholipids are able to improve culture performance of marine fish larvae in a further extend than soybean lecithin, both types of phospholipids (PL) markedly increase oxidative risk. The inclusion of a fat‐soluble antioxidant such as the vitamin E α‐tocopherol could allow a better control of oxidative stress. The objective of this study was to determine the combined effect of graded levels of α‐tocopherol with different levels and sources of krill phospholipids (KPL) and soybean lecithin (SBL) on growth, survival, resistance to stress, oxidative status, bone metabolism‐related genes expression and biochemical composition of sea bream larvae. Sea bream larvae were completely weaned at 16 dph and fed for 30 days seven microdiets with three different levels of PL (0, 40 and 80 g kg^?1 diet) and two of α‐tocopherol 1500 and 3000 mg kg^?1 diet. Sea bream larvae fed diets without PL supplementation showed the lowest survival, growth and stress resistance, whereas increase in PL, particularly KPL, markedly promoted larval survival and growth. However, feeding SBL markedly increased TBARs and GPX gene expression increasing the peroxidation risk in the larvae. Besides, KPL inclusion improved incorporation of n‐3 HUFA and, particularly, EPA into larval tissues, these fatty acids being positively correlated with the expression of BMP‐4, RUNX 2, ALP, OC and OP genes and to bone mineralization for a given larval size class. The increase in dietary α‐tocopherol tends to improve growth in relation to the n‐3 HUFA levels in the diet, denoting the protective role of this vitamin against oxidation. Indeed, dietary α‐tocopherol decreased the oxidative stress in the larvae as denoted by the reduction in larval TBARs contents and gene expression of SOD and CAT, but not GPX. Thus, increase in dietary α‐tocopherol effectively prevented the formation of free radicals from HUFA, particularly EPA, but did not affect the incidence of bone anomalies or the expression of genes related to osteogenetic processes.  相似文献   

Life table determination of thermal requirements of the tomato borer Tuta absoluta     

J. C. Martins  M. C. Picanço  L. Bacci  R. N. C. Guedes  P. A. Santana  D. O. Ferreira  M. Chediak 《Journal of pest science》2016,89(4):897-908

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Photo-Assisted Degradation,Toxicological Assessment,and Modeling Using Artificial Neural Networks of Reactive Gray BF-2R Dye     

Graziele Elisandra do Nascimento  Daniella Carla Napoleão  Polyana Karynne de Aguiar Silva  Rayany Magali da Rocha Santana  Ana Maria Ribeiro Bastos  Léa Elias Mendes Carneiro Zaidan  Maiara Celine de Moura  Luana Cassandra Breitenbach Barroso Coelho  Marta Maria Menezes Bezerra Duarte 《Water, air, and soil pollution》2018,229(12):379

This work investigates the degradation of Reactive Gray BF-2R dye (a blend of reactive yellow 145, reactive orange 122 and reactive black 5 dyes) using UV/H₂O₂, Fenton, and photo-Fenton-advanced oxidative processes, with artificial sunlight and UV-C radiations. The photo-Fenton process employing UV-C radiation was the most efficient under the conditions studied. The ideal conditions for the degradation of the dye, determined using a factorial design 2³ and a study of the concentration of hydrogen peroxide ([H₂O₂]), were [H₂O₂] equal to 40 mg L^?1, iron concentration [Fe] of 1 mg L^?1, and pH between 3 and 4. The Chan and Chu non-linear kinetic model predicted the kinetic data with a degradation of over 98% for color and 68% for aromatics after 60 min. The behavior of the chemical oxygen demand fitted the first-order kinetic model well, with a degradation of 64% after 60 min. The Multilayer Perceptron 7-11-2 artificial neural network model enabled to model the degradation process of the aromatics and accurately predict the experimental data. Toxicity tests indicated that the post-treatment samples were non-toxic for Escherichia coli bacteria, and Portulaca grandiflora and Basil sabory seeds. However, they inhibited the growth of Lactuca sativa seeds and Salmonella enteritidis bacteria. The photo-Fenton process with UV-C radiation degraded the dye studied efficiently and the degradation percentages were, on average, 7% and 5% higher for color than those observed when employing the Fenton and UV/H₂O₂ processes, respectively. With the aromatic, however, they were 84% and 62% higher, thus justifying the use of this process.  相似文献   

Identification and relationships of accessions grown in the grapevine (<Emphasis Type="Italic">Vitis vinifera</Emphasis> L.) Germplasm Bank of Castilla y Léon (Spain) and the varieties authorized in the VQPRD areas of the region by SSR-marker analysis     

J. C. Santana  E. Hidalgo  A. I. de Lucas  P. Recio  J. M. Ortiz  J. P. Martín  J. Yuste  C. Arranz  J. A. Rubio 《Genetic Resources and Crop Evolution》2008,55(4):573-583

A total of sixty-five accessions from the Spanish region of Castilla y León including those authorized in the VQPRD areas were characterized for six SSR loci. All the samples but one unknown were identified by comparison to other databases. Thirty synonymous samples and three cases of homonymy were found out, confirming in most cases ampelographic expectations. Five unique genotypes belonging to local varieties in risk of extinction were detected. Several parameters were calculated to assess the usefulness of the chosen loci in this work. A dendrogram representing the genetic similarities among the accessions was constructed using the neighbor-joining method to investigate possible parentage relationships in the sample, and to explain them from an historical and cultural point of view.  相似文献   

Introduced <Emphasis Type="Italic">Eucalyptus</Emphasis> psyllids in Brazil     

Dalva Luiz de Queiroz Santana  Daniel Burckhardt 《Journal of Forest Research》2007,12(5):337-344

In the last 10 years, four species of psyllid were detected in Brazil on eucalypts: Ctenarytaina spatulata in 1994, Blastopsylla occidentalis in 1997, Ctenarytaina eucalypti in 1998 and Glycaspis brimblecombei in 2003. The latter two are serious pests in several countries. In Brazil, G. brimblecombei caused significant damage to the eucalypt plantations in the first years of its introduction. Now this pest is under control due to the programmes of integrated pest management, where the parasitoid is the principal control agent. The four eucalypt psyllid species introduced into Brazil are presented with information on distribution, hosts, biology and control.  相似文献   

Characterization and quantification of blood cells from the umbilical cord of dogs     

Nakage AP  Santana AE  de Cápua ML  Godoy AV 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2005,34(4):394-399

BACKGROUND: Models for the study of hematopoietic stem cells in dogs provide important information for bone marrow transplantation in humans. Recent studies have reported the importance of human umbilical cord blood (UCB) as an alternative to allogenic bone marrow for hematopoietic reconstitution. However, there are no studies on the UCB cells of dogs. OBJECTIVE: The aim of this experiment was to characterize and quantify the blood cells of the umbilical cord of dogs. METHODS: The blood of the umbilical cord of 20 neonatal dogs, delivered at term, with a median gestation time of 58 days, was collected with a 5-mL syringe containing EDTA. Total RBC, WBC, and platelet counts, HCT, hemoglobin (Hgb) concentration, and RBC indices were determined using an automatic cell counter. The differential leukocyte count was determined manually in blood smears stained with May-Grünwald-Giemsa. Reticulocyte percentages were determined on blood smears stained with brilliant cresyl blue and counterstained with May-Grünwald Giemsa. RESULTS: The MCHC and numbers of RBCs, WBCs, neutrophils, and eosinophils in UCB were lower as compared with reference values for the peripheral blood of healthy neonatal and adult dogs; whereas, the MCV and reticulocyte percentages were higher. CONCLUSION: Erythrocyte macrocytosis and hypochromasia in UCB were consistent with marked reticulocytosis and indicative of high erythropoietic activity. The results of this study are an important first step in the characterization of UCB from neonatal dogs.  相似文献   

Biomechanics in young and adult italian standardbred trotter horses in real racing conditions     

Vilar JM  Spadari A  Billi V  Desini V  Santana A 《Veterinary research communications》2008,32(5):367-376

This study was undertaken to characterize and establish the kinematic standards of Italian standardbred trotter horses in real racing conditions. Certain parameters, such as the angles of both front and hind fetlock joints, stride length, stride frequency and average speed in youngs and adults are presented, examinated and compared with statistical tests. The angles and stride length were obtained using ONTRACK software over frames extracted from two CANON MV630i digital videocameras. For the stride frequency, a third identical videocamera filmed the horses to count the number of beats per minute. The average speed was calculated by multiplying the stride frequency by the stride length and transforming the result into kilometers per hour. The data reveal that as speed increases, younger horses have more extended fetlock joints than adults. Contrary to this, at the maximum speed (hereafter referred to as sprint speed) the adults increase both their stride frequency and stride length, while young horses increase stride frequency and decrease stride length. We think that the knowledge of these parameters in competition breeds could have potential relevance as they may reveal early indicators of the development of proper and adequate characteristics in young horses of those breeds.  相似文献   

Landscape makers and landscape takers: links between farming systems and landscape patterns along an intensification gradient     

Paulo?F.?RibeiroEmail author  José?L.?Santos  Joana?Santana  Luís?Reino  Pedro?J.?Leit?o  Pedro?Beja  Francisco?Moreira 《Landscape Ecology》2016,31(4):791-803

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Supplementation of pig diets in the growth and termination phases with different calcium sources     

Ana Lúcia Almeida Santana  Paulo Levi de Oliveira Carvalho  Eliseu Carlos Cristofori  Poliana Caroline da Silva Chambo  Mariana Barbizan  Ricardo Vianna Nunes  Cristine Regina Gregory  Jansller Luiz Genova 《Tropical animal health and production》2018,50(3):477-484

The aim of this study was to evaluate the effect of supplementation of pig diets in the growth and termination phases with different calcium sources. In experiment I, 36 whole males were distributed in randomized blocks in six groups, with six replications. A basal diet was formulated to meet the animals’ nutritional requirements except for calcium (0.09%), and the sources evaluated (calcitic limestone, monodicalcium phosphate, calcinated bone flour, and oyster flour) replaced the basal diet to provide 0.59% of total calcium. To determine the endogenous calcium, a diet containing low calcium (0.019%) was given simultaneously to another group of animals. Feces and urine were collected for determination the coefficients of apparent and true digestibility. In experiment II, 160 piglets were distributed in randomized blocks in four treatments, with five replications and four animals per experimental unit. Carcass and performance parameters, calcium concentration in bone and serum, and bone parameters were evaluated. The data were submitted to analysis of variance and factorial. The calcium source did not influence the digestibility coefficients determined by total collection (P > 0.05). The digestibility of Ca from oyster flour estimated by collection with an indicator was higher than that from the other sources (P < 0.05). Calcium sources did not interfere in the evaluated parameters (P > 0.05). The sources studied in this work can be used to supplement growing pigs’ diets.  相似文献