Long-term estradiol-17β administration decreases the number of neurons in the caudal mesenteric ganglion innervating the ovary in sexually mature gilts     

Koszykowska M  Calka J  Szwajca P  Jana B 《The Journal of reproduction and development》2011,57(1):62-71

The effect of estradiol-17β (E(2)) on the number and distribution of neurons in the caudal mesenteric ganglion (CaMG) supplying the ovary of adult pigs was investigated. Also, the numbers of ovarian dopamine-β-hydroxylase (DβH-), neuropeptide Y (NPY-), somatostatin (SOM-), galanin (GAL-) and estrogen receptor (ER)-immunoreactive perikarya as well as the density of the intraganglionic nerve fibers containing DβH and/or NPY, SOM, GAL were determined. E(2) was administered i.m. from day 4 of the first studied estrous cycle to the expected day 20 of the second studied cycle. Injections of E(2) (1) increased the E(2) level in the peripheral blood approximately 4-5 fold, (2) decreased the number of small-sized Fast Blue-positive postganglionic neurons in the CaMG, (3) decreased the number of small perikarya in the ventral, dorsal and central regions of the CaMG, (4) decreased the number of large perikarya in the dorsal and central regions, (5) decreased the number of small and large perikarya in the CaMG that were DβH(+)/NPY(+), (6) decreased the number of small DβH(+) but NPY(-) perikarya, (7) decreased the number of small perikarya coded DβH(+)/SOM(+) and DβH(+)/SOM(-), (8) decreased the number of small DβH(+)/GAL(-) perikarya, (9) decreased the number of small and large perikarya expressing ER subtypes α and β and (10) decreased the total number of nerve fibers in the CaMG containing DβH and/or NPY and DβH and/or GAL. These results show that long-term E(2) treatment of adult gilts downregulates the populations of both noradrenergic and ERs expressing ovarian neurons in the CaMG. Our findings suggest also that elevated E(2) levels that occur during pathological states may regulate gonadal function(s) by affecting ovary supplying neurons.  相似文献   

Diversity of S-alk(en)yl cysteine sulphoxide content within a collection of garlic (Allium sativum L.) and its association with the morphological and genetic background assessed by AFLP     

Jaroslava Ovesná  Ladislav Kučera  Jana Horníčková  Leona Svobodová  Helena Stavělíková  Jan Velíšek  Luigi Milella 《Scientia Horticulturae》2011

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Blood coagulation times in the European brown hare (Lepus europaeus)     

Pikula J  Adam V  Bandouchova H  Beklova M  Horakova J  Horakova H  Kizek R  Krizkova S  Skocovska B  Supalkova V  Svoboda M  Tremi F  Vitula F 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(4):361-363

Background:  Many causes of mortality in the European brown hare, such as bacterial and viral infections, anticoagulant poisoning, and trauma, may result in hemorrhage. There are, however, no reference values concerning blood clotting in this species.  Objectives:  The aim of this study was to determine reference values for blood coagulation times and related parameters in healthy European brown hares.  Methods:  Blood samples from 30 clinically healthy adult hares (15 males and 15 females) were obtained. Hares were physically restrained for blood collection from the cephalic vein into tubes containing citrate and EDTA.  Results:  Mean ± SD were obtained for thrombin time (TT) (13.97 ± 1.37 seconds), prothrombin time (PT) (13.32 ± 2.15 seconds), activated partial thromboplastin time (APTT) (16.73 ± 1.86 seconds), fibrinogen concentration (2.98 ± 1.06 g/L), and platelet count (355.28 ± 128.73 × 10⁹/L). Conclusions: Reference values for blood coagulation times and other parameters associated with blood clotting will be useful in the laboratory evaluation of hemorrhage in the European brown hare.  相似文献   

Few indirect effects of baculovirus on parasitoids demonstrate high compatibility of biocontrol methods against Tuta absoluta     

Jérémy Gonthier  Judit Arnó  Jörg Romeis  Jana Collatz 《Pest management science》2023,79(4):1431-1441

BACKGROUND

Combining different biocontrol agents, particularly micro- and macroorganisms, can contribute to new and sustainable pest control approaches. Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most destructive pests of solanaceous crops. An emerging management strategy consists of biological control using microbial insecticides such as baculoviruses, but with limited efficacy. Thanks to their high target specificity, baculoviruses can be used simultaneously with natural enemies such as parasitoids for improved control of T. absoluta. However, potential indirect nontarget effects of baculoviruses on parasitoids can result from overlapping resource requirements. We assessed whether ovipositing parasitoid females discriminated against virus-treated hosts and examined the outcome of within-host competition between the hymenopteran parasitoids Necremnus tutae (Reuter) (Eulophidae) and Dolichogenidea gelechiidivoris Marsch (Braconidae), and the Phthorimaea operculella granulovirus (PhopGV, Baculoviridae) that infects T. absoluta larvae.

RESULTS

Female D. gelechiidivoris discriminated against virus-treated hosts, whereas N. tutae did not. We found few indirect virus-related effects depending on the species, the sex, and the time of virus treatment. Effects were ambivalent for D. gelechiidivoris offspring and ranged from increased male longevity when infection occurred before parasitization to reduced emergence and male longevity when infection occurred after parasitization. N. tutae offspring showed a longer development time and shorter male longevity when they developed in virus-treated hosts.

CONCLUSION

The virus had a low impact on parasitoid offspring. In rare cases, adverse effects were detected; however, the low magnitude of these effects is unlikely to reduce the fitness of parasitoid offspring, therefore both parasitoids seem compatible with the baculovirus for control of T. absoluta. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Congenital subcutaneous arteriovenous malformation in a puppy: diagnosis with CT angiography          下载免费PDF全文

Pavel Proks  Ladislav Stehlik  Michaela Paninarova  Katarina Irova  Jana Lorenzova  Alois Necas 《Veterinary dermatology》2015,26(5):384-386

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Landscape diversity enhances the resilience of populations,ecosystems and local economy in rural areas     

Peter Schippers  C. Martijn van der Heide  Hans Peter Koelewijn  Marleen A. H. Schouten  René M. J. M. Smulders  Marleen M. P. Cobben  Marjolein Sterk  Claire C. Vos  Jana Verboom 《Landscape Ecology》2015,30(2):193-202

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Isolation and Characterization of Cadmium- and Arsenic-Absorbing Bacteria for Bioremediation     

Jatindra N. Bhakta  Y. Munekage  K. Ohnishi  B. B. Jana  J. L. Balcazar 《Water, air, and soil pollution》2014,225(10):1-10

The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)- and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg l^?1) for use as metal(loid) bioremediation agents. Out of 40, 4 isolates, RCd3, RCd6, RAs7, and RAs10, showed a relatively higher growth rate in Cd- or As-supplemented culture media which were selected for further study. The selected isolates showed a high minimum inhibitory concentration (60–400 mg l^?1 for Cd and 400–2200 mg l^?1 for As), which demonstrated their remarkable Cd and As resistance capabilities. The metal(loid) removal efficiencies (0.032–0.268 μg Cd h^?1 mg^?1 and 0.0003–0.0172 μg As h^?1 mg^?1 [wet weight cell]) of selected isolates indicated their greater magnitude in absorbing Cd compared to As from water. Phylogenetic analysis of the 16S rDNA sequences revealed that isolates RCd3, RCd6, RAs7, and RAs10 were closely related to Acinetobacter brisouii, Pseudomonas abietaniphila, Exiguobacterium aestuarii, and Planococcus rifietoensis, respectively. Because of high Cd and As resistance and removal efficiency, the selected isolates can survive in a high metal(loid)-contaminated environment and could be a potential tool for bioremediation of high metal(loid)-contaminated effluents to protect the aquatic environment.  相似文献   

Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments   总被引：1，自引：0，他引：1  

Guizetti J  Schermelleh L  Mäntler J  Maar S  Poser I  Leonhardt H  Müller-Reichert T  Gerlich DW 《Science (New York, N.Y.)》2011,331(6024):1616-1620

After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits by abscission. Here, we examined intermediate stages of abscission in human cells by using live imaging, three-dimensional structured illumination microscopy, and electron tomography. We identified helices of 17-nanometer-diameter filaments, which narrowed the cortex of the intercellular bridge to a single stalk. The endosomal sorting complex required for transport (ESCRT)-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments. Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site. The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures.  相似文献   

Effects of combinations of heavy metal pollutants on Cuscuta reflexa     

Sasadhar Jana  Aloke Bhattacharjee 《Water, air, and soil pollution》1988,42(3-4):303-310

The effects of combinations of Hg, As, Pb, Cu, Cd, and Cr on the variables of Cuscuta reflexa Roxb. in natural condition were studied. All of the combinations of heavy metals tested decreased Hill reaction activity, chlorophyll and protein contents and increased tissue permeability over control values. The effects were best visible with the treatments of (As + Cd + Pb + Hg + Cu + Cr) and (As + Cd + Pb + Hg + Cu), followed by (As + Cd + Pb + Hg), (As + Cd + Pb), and (As + Cd). The degree of inhibition or increase by combinations of heavy metals is much higher due to synergism as the observed effects exceed the summed amount. The general order of sensitivity was As > Cd > Pb > Hg > Cu > Cr (absolute metal concentration).  相似文献   

Isolation and identification of terpene chlorohydrins found in cold-pressed orange oil     

Weiss ER  Pika J  Braddock RJ 《Journal of agricultural and food chemistry》2003,51(8):2277-2282

Three terpene chlorohydrins found in cold-pressed orange oil were concentrated by silica adsorption chromatography and purified by preparative HPLC. Formation of these chlorohydrins was determined to be the result of a reaction of d-limonene, the major component of cold-pressed oil, with hypochlorous acid, found in chlorinated treatment water used in the oil recovery process. NMR analyses indicated that the major chlorohydrin present was the diequatorially substituted (1R,2R,4R)-2-chloro-8-p-menthen-1-ol (1). The other two compounds were the diaxial trans stereoisomer, (1S,2S,4R)-2-chloro-8-p-menthen-1-ol (2), and the dichlorohydrin, (1R,2R,4R)-2,9-dichloro-8-p-menthen-1-ol (3).  相似文献