Knowledge,attitudes, and practices (KAP) related to brucellosis and factors affecting knowledge sharing on animal diseases: a cross-sectional survey in the dry zone of Sri Lanka     

Kasthuri Arachchige Chandrika Hemali Abeyratne Kothalawala  Kohei Makita  Hemal Kothalawala  Athambawa Mohamed Jiffry  Satoko Kubota  Hiroichi Kono 《Tropical animal health and production》2018,50(5):983-989

Farmers’ lack of knowledge is assumed to have affected the presence of brucellosis in Sri Lanka for decades. This study, carried out in the Ampara district in the dry zone of Sri Lanka, revealed that there is a significant knowledge gap for brucellosis compared to foot and mouth disease (FMD) (p?<?0.001). Only 8.3% of farmers knew that brucellosis causes cattle abortions. Only 2.6% knew that it is zoonotic. The difference in knowledge of the symptoms and transmission of brucellosis and FMD was significant (p?<?0.001). Farmers’ attitudes and practices related to the spread of the disease were poor. Farmers’ education and spoken language had a negative influence on knowledge. Young people and those with strong social relationships were efficient in knowledge sharing. It can be concluded that brucellosis knowledge, attitudes, and practices are poor; thus, there is a need for more attention in disease control policymaking. Backward farmer groups should be the focus in animal health extension programs.  相似文献   

Thermal stimulation at 39°C facilitates the fusion and elongation of C2C12 myoblasts     

Satoko Hayashi  Shinichi Yonekura 《Animal Science Journal》2019,90(8):1008-1017

The aim of this study was to determine the effect of thermal stimulation at 39°C on the fusion and elongation of skeletal muscle cells. During a 5 day differentiation process of C2C12 cells, nine groups subjected to varying lengths of thermal stimulation at 39°C were established. Afterward, all groups were immunostained using anti‐muscle heavy‐chain antibody to test for myotube formation. Quantification of the myotube area demonstrated a significant increase in the group subjected to thermal stimulation at 39°C during the latter half of the differentiation compared with the control group, but the fusion index was significantly higher in the group that received hyperthermic treatment during the first half of the differentiation period. Moreover, the longitudinal length of myotubes was significantly increased in the groups that were subjected to thermal stimulation at 39°C during the latter half of the differentiation period. The distance between the center of myotubes and the nucleus farthest away from the center was substantially extended in the group receiving thermal stimulation at 39°C only on the fourth day of the differentiation. Together, these results demonstrate that thermal stimulation at 39°C facilitates myoblast fusion and elongation.  相似文献   

A canine case with cystic meningioma showing miraculous reduction of the cystic lesion     

Masae WADA  Daisuke HASEGAWA  Yuji HAMAMOTO  Atsushi ASAI  Akane SHOUJI  James CHAMBERS  Kazuyuki UCHIDA  Michio FUJITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):101-104

A 12-year-old spayed female Labrador retriever was presented with forebrain signs. Brain MRI revealed a huge cystic lesion with the thickened falx in the frontal region. The brain parenchyma surrounding the lesion showed significant signs of a mass effect and also increased intracranial pressure. However, the dog suddenly became lucid after about two weeks, and an MRI scan one month after the initial study revealed a dramatically shrunken cystic lesion. The dog survived for over a year until it was euthanized for other reasons, and the brain lesion was diagnosed as a cystic meningioma histologically. To the authors’ knowledge, this is the first report that described the reduction of the cystic lesion of a cystic meningioma in dogs.  相似文献   

Influence of sickness condition on diurnal rhythms of heart rate and heart rate variability in cows     

Masumi YOSHIDA  Ken ONDA  Yasunori WADA  Masayoshi KUWAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):375-379

Parameters of heart rate variability would explain changes in heart rate during the disease status in cows and to evaluate whether such changes might provide a more sensitive and quantitative indicator of these conditions than crude indices. For this purpose, we recorded electrocardiograms for 24 hr using a Holter-type electrocardiograph and applied power spectral analysis of heart rate variability in both five clinically healthy and four hospitalized cows. The significant findings of the current investigation were that the diurnal variations of autonomic nervous function are abolished in cows that are sick. This abnormal rhythm was induced by predominant parasympathetic inhibition in these cows. Therefore, the heart rate variability may be a useful indicator of sickness condition in cows.  相似文献   

Effect of type of aromatic nucleus in lignin on the rate of the β-O-4 bond cleavage during alkaline pulping process     

Satoko Shimizu  Tomoya Yokoyama  Yuji Matsumoto 《Journal of Wood Science》2015,61(5):529-536

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FORMATION, COMPOSITION AND STRUCTURE OF HYDROXY-ALUMINOSILICATE IONS   总被引：7，自引：0，他引：7  

SHIN-ICHIRO WADA  KOJI WADA 《European Journal of Soil Science》1980,31(3):457-467

Formation of hydroxy-Al (HA) and hydroxy-aluminosilicate (HAS) ions was studied in solutions of A1C1₃, orthosilicic acid and NaOH. Evidence for incorporation of Si into HA is that HA retarded the development of silicomolybdate colour and Si was retained by a cation-exchanger. The reaction is believed to be mainly a condensation reaction between the hydroxyl groups in Al–OH–Al and Si–OH groups. The HAS with Si/Al ratio 0.3–0.6 showed infrared spectra similar to allophane-like constituents in Ando soils.  相似文献   

Comparison of progesterone concentrations in blubber and plasma among female Antarctic minke whales of known reproductive status     

Inoue  Satoko  Yasunaga  Genta  Pastene  Luis A. 《Fisheries Science》2019,85(6):971-977

Fisheries Science - The utility of progesterone concentration in blubber as a means of determining reproductive status in the Antarctic minke whale Balaenoptera bonaerensis was assessed through a...  相似文献   

Effect of microwave decomposition on inductively coupled plasma spectrometry analysis of minerals in oysters     

Yoda  Tsuyoshi  Ichinohe  Satoko  Yokosawa  Yukihito 《Fisheries Science》2019,85(6):1089-1098

Fisheries Science - Oysters, which can be cultivated in oceans, are rich in nutrients. Recently, our group has reported the rapid analysis of multiple minerals in Crassostrea gigas oysters by...  相似文献   

Visualization of the respiring bacteria in sediments inhabited by Capitella sp. 1     

SHIOW SHYAN  WU  HIROAKI  TSUTSUMI  KUMIKO  KITA-TSUKAMOTO  KAZUHIRO  KOGURE  KOUICHI  OHWADA  MINORU  WADA 《Fisheries Science》2003,69(1):170-175

ABSTRACT:    We used the tetrazolium salt 2-( p -iodophenyl)-3-( p -nitrophenyl)-5-phenyl tetrazolium chloride (INT) to locate sites of active (respiring) bacterial populations in sediments inhabited by the deposit-feeding polychaete Capitella sp. 1. In sediment microcosms, this organism created typical protruding tubes on the sediment surface and burrows from day 1. Within a couple of hours after adding INT into the overlying water, the red color of the reduced form of INT (INT-formazan) became apparent in the water column and on the sediment surface. During the following 24 h, part of the subsurface burrows turned red and the color of formazan intensified with time during incubation. Spatial heterogeneity of the distribution of the formazan deposits along burrows also was recognized. Microscopic observations revealed that the tubes and feces produced by Capitella sp. 1, as well as detrital particles, were the sites of intensive formazan deposition. Observation with higher magnification (up to ×1250) revealed that INT-formazan was present either as bacterial intercellular deposits or as microgranules covering entire bacterial cells. In situ application of INT into a sediment microcosm provides a simple and sensitive way to visualize the impact of burrow structures created by small macrobenthos, such as Capitella , on the distribution of metabolically active bacteria.  相似文献   

Damping-off of cabbage plug seedlings caused by Pythium ultimum var. ultimum in Japan     

Masaharu Kubota  Satoko Nakasuji  Masafumi Shimizu  Kazufumi Nishi 《Journal of General Plant Pathology》2006,72(2):123-125

In October 2004, Pythium ultimum var. ultimum was isolated from rotten stems of cabbage plug seedlings in a commercial nursery in Mie Prefecture (Japan). The isolated fungus was then used to inoculate seedlings and subsequently reisolated from the seedlings with the damping-off disease, showing that P. ultimum var. ultimum is a new pathogen causing cabbage seedling disease.  相似文献