Analysis of mitochondrial DNA protein-coding region in the Yeso Sika deer (Cervus nippon yesoensis)     

Kenta WADA  Michinari YOKOHAMA 《Animal Science Journal》2004,75(4):295-302

In the present study, mitochondrial DNA sequences of the Yeso Sika deer (Cervus nippon yesoensis) were studied. Specifically, protein‐coding genes as mitochondrial NADH dehydrogenase subunits (ND1, ND2, ND3, ND4L, ND4, ND5 and ND6), cytochrome c oxidase subunits (CO I and CO III), ATP synthase subunits (ATPase8 and ATPase6) and cytochrome b. Also, phylogenetic analyses on eight mammalian species were performed, including the Muntjac deer (Muntiacus reevesi). The rate of amino‐acid substitution was lowest (3.74%) between Yeso Sika deer and Muntjac deer, and the values between Yeso Sika deer and other species (sheep, cattle, horse, pig, mouse, human and chimpanzee) were 6.63%, 7.30%, 12.55%, 13.03%, 23.59%, 24.82% and 25.04%, respectively. Among them, the highest value of divergence was recognized in ATPase8, and the second structure of ATPase8 showed a difference between the Yeso Sika deer and Muntjac deer as a result of the substitution of 34His→Tyr and 49Thr→Ile. In addition, we identified a substitution of an amino‐acid sequence (19Thr→Ala) between the Yeso Sika deer and Yakushima Sika deer (C. n. yakushimae). From these results, ATPase8 was also a variable region in Cervidae.  相似文献   

Self- and nonself recognition during hyphal interactions in Rosellinia necatrix     

Takahiro Uwamori  Kanako Inoue  Chiaki Kida  Yuichi Morita  Pyoyun Park  Hitoshi Nakayashiki  Satoko Kanematsu  Kenichi Ikeda 《Journal of General Plant Pathology》2015,81(6):420-428

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Possibility of quantitative assessment of the contribution of paddy irrigation and caldera lakes to river water in Bali Island using water isotopic physics     

Nakagiri  Takao  Kato  Hisaaki  Maruyama  Seiji  Hashimoto  Satoko  Horino  Haruhiko  Sakurai  Shinji 《Paddy and Water Environment》2019,17(3):463-473

Paddy and Water Environment - This study aims to investigate a possibility of quantitative assessment of the relationship between return flow from paddy fields and river regime by using hydrogen-...  相似文献   

Studies on trichloroethylene-extracted feeds. VII. Observations with laboratory animals fed trichloroethylene-extracted soybean oil meal     

PRITCHARD WR  SAUER F  REHFELD CE  PERMAN V  SAUTTER JH  WADA S  SCHULTZE MO 《American journal of veterinary research》1956,17(64):448-454

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Bacterial wilt of bellflower caused by Ralstonia solanacearum in Japan     

Satoko Kusumoto  Yuichi Takikawa 《Journal of General Plant Pathology》2005,71(2):158-159

A sudden wilt of bellflower (Campanula lactiflora) was observed in Japan in 1997. A bacterium that formed white fluidal and mucoid colonies resembling those of Ralstonia solanacearum was isolated from the infected plants. The bacterium was bacteriologically identified as biovar 3 of R. solanacearum. This is the first report of R. solanacearum affecting a plant species of the Campanulaceae family.  相似文献   

Light-chain multiple myeloma in a cat.     

Osamu Yamada  Kyoichi Tamura  Hiroko Yagihara  Mayu Isotani  Mari Azakami  Satoko Sawada  Kenichiro Ono  Tsukimi Washizu  Makoto Bonkobara 《Journal of veterinary diagnostic investigation》2007,19(4):443-447

A diagnosis of light-chain multiple myeloma was made in an 11-year-old male American Shorthair cat. The cat showed atypical plasma cell infiltration in the bone marrow, biclonal gammopathy caused by polymerization of myeloma protein (M-protein), and Bence-Jones proteinuria. The M-protein in the serum of the cat was analyzed by using 12% sodium dodeyl sulfate (SDS) polyacrylamide gel electrophoresis with Coomassie brilliant blue staining. An intense band with a size of 27 kDa, the size of the immunoglobulin light chain, was clearly observed, whereas the band corresponding to the immunoglobulin heavy chain (59 kDa) was undetectable. The 27-kDa band was confirmed to be an immunoglobulin light chain by Western blotting by using antibodies for feline immunoglobulin. These data suggested that the neoplastic plasma cells produce light chain only, leading to the diagnosis of light-chain multiple myeloma in the cat.  相似文献   

Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt     

Samy METWALLY  Rania HAMADA  Alsagher O. ALI  Hassan Y.A.H. MAHMOUD  Nabil M. BAKER  Adel E. A. MOHAMED  Satoshi WADA  Yasunobu MATSUMOTO  Yoko AIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1676

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, the most common neoplastic disease of cattle worldwide and a serious problem for the cattle industry. Previous studies have shown the molecular prevalence of BLV and the coexistence of BLV genotype-1 and -4 in Egyptian dairy cattle; however, the molecular characteristics of BLV in Egyptian beef cattle are unknown. Therefore, we collected blood samples of 168 beef cattle from slaughterhouses in three governorates in Egypt. Based on BLV-CoCoMo-qPCR-2 targeting long terminal repeats and nested PCR targeting the env-gp51 gene, the BLV provirus infection rates were found to be 47/168 (28.0%) and 42/168 (25.0%), respectively. Phylogenetic analysis based on 501 bp of the BLV env-gp51 gene from 42 BLV isolates revealed that at least six distinctive strains (b, e, f, g, x, and z) were prevalent in cattle across the examined regions. Furthermore, phylogenetic analysis of the 420 bp sequence of the BLV env-gp51 region of the six strains against 11 known genotypes showed that the strains b, e, f, and g were clustered into genotype-1, and strains x and z were clustered into genotype-4. Our results also indicated that strains b and x exist in both dairy and beef cattle in Egypt. The present study is the first to detect and genotype BLV among beef cattle in Egypt.  相似文献   

Genotyping of infectious bronchitis viruses isolated in Japan during 2008−2019     

Masaji MASE  Makiko GOTOU  Daisuke INOUE  Satoko WATANABE  Hiroshi ISEKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):522

Seventeen isolates of infectious bronchitis virus (IBV) were obtained from various prefectures of Japan during 2008–2019 and genetically analyzed. The IBV isolates were classified into six genetic groups, based on phylogenetic analysis of the S1 gene. The S1 genotypes were distinguishable by a newly developed restriction fragment length polymorphism (RFLP) method using three endonucleases, Hae II, Hpa I, and Fok I. Moreover, the isolates were classified into four genetic groups, based on phylogenetic analysis of the S2 gene. However, novel genetic groups based on a combination of S1 and S2 genotypes, which were undetected previously, were confirmed in this study, indicating that various recombinant IBV strains were prevalent in poultry in Japan.  相似文献   

The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis     

Yamato SAJIKI  Satoru KONNAI  Reiko NAGATA  Satoko KAWAJI  Hayato NAKAMURA  Sotaro FUJISAWA  Tomohiro OKAGAWA  Naoya MAEKAWA  Yukinari KATO  Yasuhiko SUZUKI  Shiro MURATA  Yasuyuki MORI  Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162

Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease.  相似文献   

Efficacy and safety of 0.0584% hydrocortisone aceponate topical spray and systemic oclacitinib combination therapy in dogs with atopic dermatitis: a randomized,double-blinded,placebo-controlled trial     

Junko Takahashi  Satoko Kanda  Ichiro Imanishi  Tadashi Hisano  Teruyasu Fukamachi  Norihito Taguchi  Shoko Momiyama  Satoshi Nishiyama  Tomoki Motegi  Keita Iyori 《Veterinary dermatology》2021,32(2):119-e25

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