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31.
July 2004, a cow with clinical signs of ovine herpesvirus type-2 infection which is known as sheep associated malignant catarrhal fever (SA-MCF) was reported in Samsun Province in Turkey. Blood samples were collected from the suspected cow, 10 sheep housed with it, and from 150 healthy sheep and 29 healthy cattle randomly selected from different places in Samsun Province. Nested polymerase chain reaction (n-PCR) was used to detect ovine herpesvirus type-2 (OvHV-2) DNA in the suspected cow and competitive- ELISA (c-ELISA) kits were used to detect antibodies against OvHV-2. The suspected cow was found to be n-PCR positive and c-ELISA negative. The serological results were as follows: All 10 (100%) of sheep housed with the suspected cow and 18 of 29 (62%) of the randomly selected cattle were found seropositive. All 150 randomly selected healthy sheep were seronegative. The overall percentage of seropositivity was 14.7% (28/190). OvHV-2 DNA was detected in the peripheral blood leucocyte (PBL) samples of the cow and of the 10 sheep housed with the suspected cow.  相似文献   
32.
The objective of this study is to investigate the effects of dietary protein and lipid levels on the growth performance and bioeconomic benefits of two-banded seabream (Diplodus vulgaris) juveniles, a candidate species for aquaculture sector. Eight experimental diets were formulated with four protein (50, 45, 40 and 35 %) levels for each of the two lipid levels (15 and 10 %). Triplicate groups of juvenile fish with an average initial body weight of ~3.64 g were reared in a recirculating aquaculture system and hand fed twice a day until satiation for a period of 60 days. In the experiment, no difference in survival rate was found between the different groups. Relative growth rate (RGR), specific growth rate (SGR), feed conversion ratio (FCR) and daily feed intake were not significantly affected by increasing protein and/or lipid treatments in this present study. However, the RGR, SGR and FCR values showed slightly better efficiency in the experimental group (35/15) fed with lower protein content (35 %) and higher lipid level (15 %) compared with those fed other diets. According to bioeconomic analyses results, the diet with the 35 % protein and 15 % lipid generated the best profit. The results suggest that two-banded seabream can be accepted as a promising alternative species for the aquaculture industry and optimum growth of two-banded seabream fingerlings can be obtained when they are fed a diet containing 35 % crude protein and 15 % crude lipid.  相似文献   
33.
Crimean-Congo hemorrhagic fever virus threatens human health. Exposure of the infected tick is a strong risk factor for human disease. In this study, the hard ticks collected from a variety of mammalian species (cattle, sheep, goat, and buffalo) and a turtle in either coastal or inland Black Sea region of Turkey were surveyed for the presence of antigen from Crimean-Congo hemorrhagic fever virus (CCHFV). CCHFV antigen was found in 46 of 421 tick pools (10.92%). Positivity rates for the provinces varied and were as follows: Samsun 33.87%, Ordu 4.34%, Giresun 8.86%, Sinop 6.09%, Amasya 7.40%, Tokat 5.08%, and Sivas 8.06%. CCHFV antigen was detected in seven of 11 tick species tested. These results suggest that these hard ticks may act as a reservoir for CCHFV in northern Turkey.  相似文献   
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