Experimental study of the effect of 6-methyl-prednisolone on ingestion-microbicidal function activity in dog neutrophils     

J M Molina  A Garrido  A Anguiano  A Uceda  E Serrano 《Comparative immunology, microbiology and infectious diseases》1991,14(1):21-29

The effect of increasing doses of 6-methyl-prednisolone on the ingestion-microbicidal function activity of dog neutrophils was studied. Histochemical and spectrophotometrical NAT reduction techniques, together with a direct overall method using C. albicans as a test micro-organism were carried out. Samples were analysed 2 and 48 h after inoculation of different doses. The histochemical NAT technique was not very precise, but quantification of responses using the spectrophotometric method revealed that at 48 h.p.i. neutrophil reducing activity declines continuously. At 2 h.p.i. in the first few days of the experiment (i.e. at lower doses), NAT reduction increased, but subsequently fell back to O.D. levels below those recorded at 48 h.p.i. Ingestion and destruction of C. albicans followed the same pattern as that recorded for NAT activity. Simple and differential leukocyte counts performed in all samples revealed leukocytosis (with neutrophilia and lymphopaenia) at 2 h.p.i. This returned to normal levels in the intervals between inoculations.  相似文献   

Usefulness of estimated surface area of damaged skin as a proxy of mite load in the monitoring of sarcoptic mange in free-ranging populations of Iberian wild goat, Capra pyrenaica     

Pérez JM  Granados JE  Sarasa M  Serrano E 《Veterinary parasitology》2011,176(2-3):258-264

In this study we examined the influence of several factors, like the surface of skin with lesions attributable to Sarcoptes scabiei, the time of year and host sex and age on variations of mite load in Iberian wild goats, Capra pyrenaica, from the Sierra Nevada Natural Space, southern Spain. As well, the surface area of skin with scabietic lesions estimated during field surveys were compared with measurements taken in the laboratory and the potential for using categories based on surface areas estimated during monitoring and management programs was analyzed. The surface area of the skin lesions measured in the laboratory and the time of year were the main factors affecting mite load. Results also revealed discrepancies between the surface area of lesions estimated in the field and those measured in the laboratory. The highest error percentages were associated with lesions ranging between 25 and 75% of host skin surface area. By reducing the number of categories of mange infestation (from 5 to 3) the proportion of cases correctly classified using data from field surveys increases. Our analyses suggest that the estimated surface areas of scabietic lesions is a useful relative index of mite abundance and/or intensity of parasitation in Iberian wild goat. Therefore, we conclude that revised classifications should be used in the monitoring of sarcoptic mange in wild populations, since data can be obtained in an inexpensive, fast and non-invasive fashion.  相似文献   

Negative effect of the arthropod parasite, Sarcoptes scabiei, on testes mass in Iberian ibex, Capra pyrenaica     

Sarasa M  Serrano E  Soriguer RC  Granados JE  Fandos P  Gonzalez G  Joachim J  Pérez JM 《Veterinary parasitology》2011,175(3-4):306-312

Testes mass is a key factor in male reproductive success and is potentially exposed to so-called 'parasitic castration'. This is the result of the direct destruction or alteration of reproductive cell lineages (parasitic castration sensu stricto), or the indirect detrimental effects - for example, via body condition - on the ability of progenitors to produce or rear offspring (parasitic castration sensu lato). There are enormous gaps in our knowledge on the effects of parasites on the testes of wild mammals and in an attempt to rectify this dearth of data we examined the relationship between the skin parasite Sarcoptes scabiei and testes mass in Iberian ibex Capra pyrenaica. We considered data from 222 males that were culled in the population from the Sierra Nevada in Spain. Our results provide evidence that sarcoptic mange is associated with reduced size-corrected testes mass in Iberian ibex which supports the hypothesis that parasitism is a determining factor in gonad plasticity in male mammals. We discuss several hypothetical causes of this relationship and highlight the need to deepen the sub-lethal effects of pathogens if we are to accurately understand their modulator effects on host population dynamics.  相似文献   

Influence of sex and castration on growth performance and carcass quality of crossbred pigs from 2 Large White sire lines     

Morales JI  Cámara L  Berrocoso JD  López JP  Mateos GG  Serrano MP 《Journal of animal science》2011,89(11):3481-3489

In total, 360 pigs slaughtered at 125 kg of BW and destined for the dry-cured industry were used to study the influence of sex and castration [immunocastrated males (IMC), surgically castrated males (CM), and intact females (IF)] in 2 terminal Large White sire lines [Top York (TY) and Tempo (TE)] on growth performance and carcass and meat quality. The female line was Large White × Landrace. The IMC pigs were immunized against gonadotropin-releasing factor (GnRF) with Improvac at 78 (experimental d 16) and 126 (experimental d 64, 48 d before slaughter) d of age. Each of the 6 treatments was replicated 6 times (10 pigs/pen). Through the day of the first Improvac injection (62 to 78 d of age), IMC and IF grew at a slower rate (P < 0.001) than CM with no differences (P = 0.95) in feed intake. The CM had greater G:F than IF, with IMC being intermediate (P < 0.01). From 78 to 126 d of age (between Improvac injections), the IMC pigs ate less feed (P < 0.001) and grew at a slower rate than CM, with IF being intermediate. The IMC pigs were more feed efficient than IF, and both were more efficient than CM (P < 0.001). However, from 126 to 174 d of age (from the second Improvac injection to slaughter), IMC pigs grew at a faster rate (P < 0.001) and were more efficient (P < 0.001) than IF and CM. Overall, IMC and CM pigs grew at a faster rate (P < 0.01) than IF, and IMC were more efficient than the other sexes (P < 0.001), but had reduced (P < 0.001) carcass yield compared with CM and IF. The IMC and IF pigs also had less (P < 0.001) backfat depth than CM. Intact females had greater (P < 0.01) loin yield but less intramuscular fat than IMC and CM and greater (P < 0.05) fresh and trimmed ham yields than CM, with IMC being intermediate. Crossbreds from TE sires grew at a faster rate (P < 0.001) than crossbreds from TY sires, but no differences (P = 0.23 and 0.14, respectively) were found for feed intake or efficiency. Crossbreds from TY produced greater (P < 0.05) fresh and trimmed ham yield, but less (P < 0.01) loin yield and intramuscular fat content than crossbreds from TE. In conclusion, IMC pigs are more efficient, but have less carcass yield than CM and IF. The intramuscular fat content is least for IF and similar for IMC and CM. Crossbreds from TE sires were larger and had greater intramuscular fat content, but slightly less trimmed ham yield compared with crossbreds from TY sires. Immunocastrated pigs can replace CM for the production of heavy pigs destined for the dry-cured industry. Because of increased carcass weight, crossbreds from TE sires may have an advantage over crossbreds from TY sires.  相似文献   

Evaluation of genotype and environment effects on taste and aroma flavor components of Spanish fresh tomato varieties   总被引：1，自引：0，他引：1  

Cebolla-Cornejo J  Roselló S  Valcárcel M  Serrano E  Beltrán J  Nuez F 《Journal of agricultural and food chemistry》2011,59(6):2440-2450

Taste and aroma related compounds have been analyzed in a collection of four traditional varieties and two tomato hybrids, representing a wide variability in fruit shape and color, grown in different environments: screenhouse and open field. Protected cultivation tended to show lower sugar concentration (fructose and glucose) but similar acid contents (citric, malic, and glutamic acids). The decreased levels of sucrose equivalents and the similar ratios of sucrose equivalents to citric or glutamic acid contents indicated that protected cultivation, despite being useful to reduce the incidence of pests and viral diseases, reduces the organoleptic quality. Additionally, it doubles the interaccession variability and increased the level of intra-accession variability. In the case of aroma, the genotypic effect was considerably higher than the environmental component on the 12 main volatiles analyzed. Only hexanal and methyl salicylate were significantly affected by environment, while 10 out of 12 volatiles were affected by the genotype. Biplot analysis showed that, even in considerably different environments, it is possible to identify genotype-dependent main aroma profiles. In the case of 13 background volatiles, the environment showed no significant effects and the genotypic effect was lower, though it is possible to identify genotypic trends in background notes.  相似文献   

Interstellar matter and chemical evolution     

Peimbert M  Serrano A  Torres-Peimbert S 《Science (New York, N.Y.)》1984,224(4647):345-350

The determination of chemical abundances of the interstellar medium is related to three different areas of astronomy: pregalactic conditions, stellar evolution, and chemical evolution of galaxies. The observed abundances permit the testing of theories associated with these areas. Knowledge of the abundances of helium, carbon, nitrogen, and neon relative to the abundance of hydrogen in the interstellar medium puts limits on current models of cosmology, stellar evolution, and galactic evolution.  相似文献   

Dopa and dopamine in glusulase: possible artifact in studies on catecholamine metabolism     

A H Anton  A Serrano  T B Tjandramaga  L I Goldberg 《Science (New York, N.Y.)》1973,182(107):60-61

Relatively high concentrations of dopa and dopamnine were found in Glusulase, an enzyme preparation widely used in studies on catecholamine metabolism. This contamination may be a source of error in some studies, particularly in those measuring the endogenous concentrations of these catechols and their metabolic products.  相似文献   

Beneficial Effect of Melatonin on Blastocyst In Vitro Production from Heat‐Stressed Bovine Oocytes     

A Cebrian‐Serrano  I Salvador  E Raga  A Dinnyes  MA Silvestre 《Reproduction in domestic animals》2013,48(5):738-746

Melatonin may play an important role in protecting gametes and embryos from the potential harmful effects of oxidative stress. In this study, we first examined two different heat stress (HS) treatments for in vitro oocyte maturation (Experiment 1: 38.5 vs 41.0°C, during the first 20 h; Experiment 2: 38.5 vs 41.5°C, during the entire period) on bovine oocyte maturation and embryo development. Second, we tested different melatonin concentrations added to the maturation and culture medium (Experiment 3: 0, 10^?12, 10^?9, 10^?4 m ; Experiment 4: 0, 10^?3 m ), both with and without HS (38.5 or 41.5°C, respectively). In Experiment 1, the HS treatment resulted in a lower maturation rate and number of cells/blastocyst (C/B) and a higher blastocyst rate than that in the control group. In Experiment 2, oocytes/embryos from heat‐stressed oocytes (HSO) had a lower maturation, cleavage and blastocyst rates, as well as a lower C/B compared with the control. In Experiment 3, in HSO groups, 10^?4 m melatonin resulted in an increased blastocyst rate compared with 0 m melatonin, with a similar blastocyst rate to the non‐HSO without melatonin. Melatonin did not have any effect in embryos from non‐HSO groups compared with the control. In Experiment 4, 10^?3 m melatonin produced lower cleavage and blastocyst rates in HSO and lower blastocyst rate in non‐HSO when compared to melatonin‐untreated oocytes/embryos. In conclusion, 10^?4 m melatonin was found to alleviate bovine oocytes from the harmful effects of HS.  相似文献   

Plasma Concentrations of Pregnancy‐Associated Glycoproteins Measured Using Anti‐Bovine PAG‐2 Antibodies on Day 120 of Gestation Predict Abortion in Dairy Cows Naturally Infected with Neospora caninum     

I García‐Ispierto  S Almería  B Serrano  NM de Sousa  JF Beckers  F López‐Gatius 《Reproduction in domestic animals》2013,48(4):613-618

The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy‐associated glycoprotein‐2 (PAG‐2) concentrations throughout pregnancy and (ii) whether plasma PAG‐2 concentrations could predict abortion in N. caninum‐infected cows. The study was performed on a commercial Holstein‐Friesian dairy herd in northeastern Spain and the final data included those recorded in 53 non‐aborting and 19 aborting animals. Blood samples were collected immediately before pregnancy diagnosis (on Days 40, 90, 120, 150, 180 and 210 post‐insemination) in non‐aborting cows or until the time of abortion detection in aborting cows. General lineal models (GLM) repeated measures anova revealed the different behaviour of PAG‐1 and PAG‐2, and significant effects of Neospora seropositivity, cool season and twin pregnancy on plasma PAG‐2 concentrations throughout gestation (between‐subject effects). In addition, based on the odds ratios, the likelihood of abortion increased in Neospora‐seropositive cows (by a factor of 7.0) compared to seronegative animals and decreased in cows with a high plasma PAG‐2 concentration (>4.5 ng/ml) on Day 120 of pregnancy (by a factor of 0.24), compared to the remaining cows. In conclusion, there is a relationship between plasma PAG‐2 concentrations and the risk of abortion in Neospora‐infected dairy cows. Thus, plasma PAG concentrations measured using anti‐boPAG‐2 antiserum on Day 120 of gestation could serve as an indicator of the abortion risk in N. caninum infected animals; values <4.5 ng/ml indicating a high risk of abortion in chronically infected animals.  相似文献   

Calibration of a capacitance probe for measurement and mapping of dry matter yield in Mediterranean pastures     

João M. Serrano  José O. Peça  J. Marques da Silva  Shakib Shahidian 《Precision Agriculture》2011,12(6):860-875

The main objective of this study was to calibrate a commercial capacitance probe for measuring pasture dry matter yields under Mediterranean conditions. The standard method of assessing pasture biomass is based on cutting all the forage within a specified area and requires great effort and expense to collect enough samples to accurately represent a pasture. The field tests were carried out in 2007, 2008 and 2009 on different dates (phenological stages), and on five dairy farms, representing typical pastures in the region (grasses; legumes; and bio-diverse flora, mixture of grasses, legumes and others species). The linear regression techniques used in 2007 to relate the weight of the herbage (direct measurements) to the meter reading of capacitance (indirect measurements) led to high regression coefficients in grasses (R² = 0.90; P < 0.01) and heterogeneous botanical composition (R² = 0.87; P < 0.001) and moderate regression coefficient in legumes species (R² = 0.48; P < 0.05). The validation of the calibration equations in 2008 and 2009 in two sites showed RSME values of 130 kg ha⁻¹ in heterogeneous botanical composition and 456 kg ha⁻¹ in legumes. The results indicated that the capacitance probe together with a GPS receiver might support site-specific management of pastures which would be useful in large areas.  相似文献