Environmental influences on late-summer individual Japanese eel diel activity and space utilization patterns in a shallow western Japan brackish lake     

Itakura  Hikaru  Miyake  Yoichi  Wakiya  Ryoshiro  Kimura  Shingo 《Fisheries Science》2022,88(1):29-43

The late-summer diel activity patterns and space utilization of five Japanese eels, Anguilla japonica, were investigated using an acoustic telemetry positioning system in a shallow brackish lake in western Japan. Eels were highly nocturnal and crepuscular, with 98.1% of positions detected between sunset and sunrise, and were more active during dusk and dawn compared with nighttime, and during full moons compared with other lunar periods. Most diurnal activity occurred during darker conditions, and two individuals extended their activity into the morning, suggesting that overcast skies reduced light intensity and prolonged nocturnal activity into daytime. Individual eels had different home-range patterns, but appeared to rest nearshore during the day, with space utilization expanding offshore at dusk and night, followed by returning to nearshore at dawn. Their home ranges and core activity areas were bordered by the shoreline, with eels tending to exhibit small activity spaces and strong site fidelity. This study provides valuable ecological knowledge about the individual behavior of eels inhabiting a small brackish lake at the northern limit of the natural distributional range of the species along the Sea of Japan, which can contribute to management and conservation of the species.

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Effects of feeding early‐harvested orchardgrass–perennial ryegrass mixed silage instead of heading stage harvested timothy silage on digestion and milk production in dairy cows     

Makoto Miyaji  Akira Yajima  Shingo Tada  Kenji Sudo  Yasuhiro Aoki 《Animal Science Journal》2020,91(1)

We evaluated the effects of replacement of heading stage harvested timothy silage with early‐harvested orchardgrass–perennial ryegrass mixed (OP) silage while maintaining or reducing concentrate input on dry matter intake (DMI), milk production, nutrient digestibility, and N balance in dairy cows. Nine multiparous Holstein cows were used in a replicated 3 × 3 Latin square design with three dietary treatments: TYL, a diet containing timothy silage where forage‐to‐concentrate ratio (FC) was 50:50; OPL, a diet containing OP silage where FC ratio was 50:50; and OPH, a diet containing OP silage where FC ratio was 60:40. We observed that an equal replacement of timothy with OP silage increased DMI, milk yield, milk protein production, and nutrient digestibility but decreased milk fat content (TYL versus OPL). We observed that replacing timothy with OP silage while reducing concentrate input increased milk fat and protein yield, nutrient digestibility, and feed efficiency and reduced urinary N loss with no effect on DMI or milk fat content (TYL versus OPH). These results show that replacing timothy with OP silage can be a good approach to improve milk production, feed efficiency, and N utilization and reduce concentrate input. However, milk fat depression should be considered when an equal substitution is performed.  相似文献   

Prokinetic effect of the 5-HT4R agonist mosapride on canine gastric motility     

Tsukamoto A  Ohno K  Maeda S  Nakashima K  Fukushima K  Fujino Y  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1635-1637

We assessed prokinetic action of gastroprokinetic agent, mosapride in dogs. Open-label cross-over study. Six healthy beagles were administered single oral mosapride at doses of 0.5, 0.75, 1, and 2 mg/kg 30 min prior to feeding, followed by 1-week interval. The motility index (MI) of gastric contraction was ultrasonographically evaluated by change rate of antral area and contraction number. Significant increases in MI were observed at doses of 0.75 mg/kg (mean ± SEM, 11.11 ± 0.19), 1 mg/kg (11.65 ± 0.34), and 2 mg/kg (12.04 ± 0.34), compared with that of the control (9.37 ± 0.51). Mosapride administration (2.0 mg/kg, BID) for 1 week had no adverse effects on blood tests or health of the animals. In conclusion, 0.75 to 2 mg/kg of mosapride produces gastric prokinetic actions without adverse effects.  相似文献   

Production of calcium maintenance factor Stanniocalcin-1 (STC1) by the equine endometrium during the early pregnant period     

Kikuchi M  Nakano Y  Nambo Y  Haneda S  Matsui M  Miyake Y  Macleod JN  Nagaoka K  Imakawa K 《The Journal of reproduction and development》2011,57(2):203-211

A factor responsible for progression to pregnancy establishment in the mare has not been definitively characterized. To identify factors possibly involved in the establishment of equine pregnancy, the endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19 and 25 pregnant animals. From initial subtractive hybridization studies, a calcium regulating factor, Stanniocalcin-1 (STC1) mRNA, was found as a candidate molecule expressed uniquely in the pregnant endometrium. Endometrial expression of STC1 mRNA was noted on day 19 and was markedly increased in the day 25 gravid endometrium. STC1 protein was found in the extracts of day 25 gravid endometrium and immunochemically localized in the uterine glands. In addition, STC1 protein was detected in uterine flushing media collected from day 25 pregnant mares. High concentrations of estradiol-17 β (E(2)) were detected in day 25 conceptuses. E(2) levels were much higher in the gravid endometrium than in other regions, whereas progesterone levels did not differ among the samples from different endometrial regions. Expression of STC1 mRNA, however, was not significantly upregulated in cultured endometrial explants treated with various concentrations of E(2) (0.01-100 ng/ml) with or without 10 ng/ml progesterone. These results indicate that an increase in STC1 expression appears to coincide with capsule disappearance in the conceptus, and suggest that STC1 from the uterine glands likely plays a role in conceptus development during the pregnancy establishment period in the mare.  相似文献   

Effect of recombinant canine interferon‐γ on granulocyte–macrophage colony‐stimulating factor,transforming growth factor‐β and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK)     

Sanae Shibata  Sadatoshi Maeda  Naho Kondo  Akiko Inoue  Shingo Maeda  Naoki Chimura  Tsuneo Fukata 《Veterinary dermatology》2011,22(1):24-30

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Quantification of chemokine and chemokine receptor gene expression in duodenal mucosa of dogs with inflammatory bowel disease     

Maeda S  Ohno K  Nakamura K  Uchida K  Nakashima K  Fukushima K  Tsukamoto A  Goto-Koshino Y  Fujino Y  Tsujimoto H 《Veterinary immunology and immunopathology》2011,144(3-4):290-298

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Homozygosity of single nucleotide polymorphisms in the 3′ region of the canine estrogen receptor 1 gene is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas     

Noritoshi KAWATE  Makoto TSUJI  Shingo HATOYA  Toshio INABA  Hiromichi TAMADA 《Animal Science Journal》2011,82(3):390-395

Differences in the distribution of single nucleotide polymorphisms (SNPs) and haplotypes in the estrogen receptor α gene (ESR1) were examined in Miniature Dachshunds (n = 48), Chihuahuas (n = 20) and Toy Poodles (n = 18). Five DNA fragments located in the 40‐kb region at the 3′ end of ESR1 were amplified by polymerase chain reaction and were directly sequenced. We compared allele, genotype and estimated haplotype frequencies at each SNP in the 3′ end of ESR1 for these three breeds of small dog. The frequency of the major allele and the genotype frequency of the major allele homozygotes, were significantly higher in Toy Poodles for five SNPs (SNP #5, #14–17) than in Miniature Dachshunds, and significantly higher in Toy Poodles than Chihuahuas for three SNPs (SNP #15–17). A common haplotype block was identified in an approximately 20‐kb region encompassing four SNPs (SNPs # 14–17). The frequencies of the most abundant estimated haplotype (GTTG) and GTTG homozygotes were significantly higher in Toy Poodles than in the other two breeds. These results imply that homozygosity for the allele, genotype and haplotype distribution within the block at the 3′ end of ESR1 is greater in Toy Poodles than in Miniature Dachshunds and Chihuahuas.  相似文献   

Venting of carbon dioxide-rich fluid and hydrate formation in mid-okinawa trough backarc basin     

Sakai H  Gamo T  Kim ES  Tsutsumi M  Tanaka T  Ishibashi J  Wakita H  Yamano M  Oomori T 《Science (New York, N.Y.)》1990,248(4959):1093-1096

Carbon dioxide-rich fluid bubbles, containing approximately 86 percent CO(2), 3 percent H(2)S, and 11 percent residual gas (CH(4) + H(2)), were observed to emerge from the sea floor at 1335- to 1550-m depth in the JADE hydrothermal field, mid-Okinawa Trough. Upon contact with seawater at 3.8 degrees C, gas hydrate immediately formed on the surface of the bubbles and these hydrates coalesced to form pipes standing on the sediments. Chemical composition and carbon, sulfur, and helium isotopic ratios indicate that the CO(2)-rich fluid was derived from the same magmatic source as dissolved gases in 320 degrees C hydrothermal solution emitted from a nearby black smoker chimney. The CO(2)-rich fluid phase may be separated by subsurface boiling of hydrothermal solutions or by leaching of CO(2)-rich fluid inclusion during posteruption interaction between pore water and volcanogenic sediments.  相似文献   

Effect of Platelet-Rich Plasma-Incorporated Gelatin Hydrogel Microspheres and Subchondral Drilling on Equine Cartilage Defects     

Nao Tsuzuki  Noe Oshita  Jong-pil Seo  Kazutaka Yamada  Shingo Haneda  Hidefumi Furuoka  Yasuhiko Tabata  Naoki Sasaki 《Journal of Equine Veterinary Science》2014

We evaluated the effect of platelet-rich plasma-incorporated gelatin hydrogel microsphere (PRP/GM) together with subchondral drilling for cartilage regeneration in horses. Cartilage defects (diameter, 4.5 mm; depth, 3.0 mm) were created in both third carpal bones of six Thoroughbred horses. A subchondral hole was drilled at the center of this cartilage defect (diameter, 2.0 mm; depth, 35 mm) in one randomly selected carpal joint (test joint), whereas no subchondral hole was made in the contralateral joint (control joint). Thereafter, the test joint was injected with PRP/GM and the control joint with saline-infused GM. Additional injections of PRP/GM were given 14 and 28 days after surgery. After 16 weeks, histologic analysis was conducted. The macroscopic and histologic scores of the test group were significantly higher than those of the control group (P < .05). These data indicated that the application of PRP/GM together with subchondral drilling accelerated cartilage regeneration in horses.  相似文献   

An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro     

Jinghui LIU  Mohamed A. MAREY  Rasoul KOWSAR  Nina HAMBRUCH  Takashi SHIMIZU  Shingo HANEDA  Motozumi MATSUI  Motoki SASAKI  Hiroyuki HAYAKAWA  Christiane PFARRER  Akio MIYAMOTO 《The Journal of reproduction and development》2014,60(5):342-348

We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in cultured bovine oviduct epithelial cells (BOECs) and AGP protein was detected in oviduct fluid. Preexposure of PMNs to AGP at physiological levels impaired PMN phagocytosis for sperm and superoxide generation. The desialylation of AGP eliminated these suppressive effects of AGP on PMN. Scanning electron microscopy revealed that AGP drastically reduced the formation of DNA-based neutrophil extracellular traps (NETs) for sperm entanglement. Additionally, AGP dose-dependently stimulated BOECs to produce prostaglandin E₂ (PGE₂) which has been shown to partially contribute to the regulation of sperm phagocytosis in the bovine oviduct. AGP and PGE₂ at concentrations detected in the oviducts additively suppressed sperm phagocytosis by PMNs. These results provide evidence that locally produced AGP may be involved in protecting sperm from phagocytosis by PMNs in the bovine oviduct.  相似文献