Primary sinonasal malignant melanoma with systemic metastasis in a non-gray horse     

Hitoshi Hatai  Takashi Hatazoe  Haruka Seo  Teruaki Tozaki  Shingo Ishikawa  Noriaki Miyoshi  Kazuhiro Misumi  Seiji Hobo 《Journal of veterinary diagnostic investigation》2021,33(2):379

A 27-y-old Anglo-Arabian gelding with bay coat color was presented with a swelling of the left maxillary region. Fenestration on the left maxilla revealed that the left maxillary sinus was filled with black-red tissue. A portion of the tissue was excised and diagnosed histologically as malignant melanoma. Genotyping of the STX17 gene for gray coat color revealed that the horse did not have the “gray” factor. The horse was euthanized ~3 mo after first presentation. During autopsy, a black-to-gray mass extended from the left nasal cavity to the surrounding paranasal sinus and invaded the hard palate, cribriform plate, and the cranial portion of the left olfactory bulb. Moreover, identical black nodules were present in lymph nodes from the mandible to the larynx, and in the spleen, liver, kidney, and adrenal glands. However, masses were not found in the skin, perineal region, or pelvic cavity. All of the black-to-gray nodules were malignant melanomas that were histologically identical to the initial biopsy; tumor emboli were also found in the kidney. Sinonasal mucosal melanoma is a rare disease in horses.  相似文献   

Nitrate Loss for Denitrification during High Frequency Research in Floodplain Groundwater of the Tama River     

Tsushima  Kouji  Ueda  Shingo  Ogura  Norio 《Water, air, and soil pollution》2002,137(1-4):167-178

This study aims to identify the possible roles of a floodplain inbiogeochemical nitrogen cycles, based on analysis of groundwaterdynamics of inorganic nitrogen species. Groundwater samples collected from boreholes made of poly vinyl chloride located onthe floodplain in the middle reach of the Tama River, Nagata district were analyzed for dissolved organic carbon, dissolved oxygen, inorganic nitrogen compounds, major anions and the stableisotope ratio of nitrate-nitrogen.Dissolved oxygen in groundwater samples collected from marked borehole (G-5) was low (50 ± 16 μM) and nitrate decreasedfrom 63 μM to < 10 μM during our research period. Concentrations of nitrate, nitrite and nitrous oxide decreased one after the other. These decreases in concentration, combinedwith ¹⁵N-enrichment of groundwater nitrate suggest denitrification as a significant nitrate sink, yielding an isotopic fractionation of nitrate-nitrogen with an enrichment factor (ε= –17.9‰) that is comparable tothose in various soil and groundwater systems. This study provesthat floodplains can perform as nitrogen sinks for groundwaterin river catchments.  相似文献   

Sequential extraction methods for soil organic nitrogen     

Rumiko KODASHIMA  Noriharu AE  Noriko KIKKOUJI  Shingo MATSUMOTO 《Soil Science and Plant Nutrition》2006,52(1):134-134

(pp. 825–831)
This study was carried out to clarify the effects of soil nitrate before cultivation and amounts of basal-dressed nitrogen on additional N application rate and yields of semi-forced tomato for three years from 1998 to 2000. The amounts and timing of additional N dressing were determined based on diagnosis of petiole sap nitrate. The top-dressing was carried out with a liquid fertilizer when the nitrate concentration of a leaflet's petiole sap of leaf beneath fruit which is 2–4 cm declined below 2000 mg L⁻¹.
For standard yield by the method of fertilizer application based on this condition, no basal-dressed nitrogen was required when soil nitrate before cultivation was 150 mg kg⁻¹ dry soil or higher in the 0–30 cm layer; 38 kg ha⁻¹ of basal-dressed nitrogen, which corresponds to 25% of the standard rate of fertilizer application of Chiba Prefecture, was optimum when soil nitrate before cultivation was 100150 mg kg⁻¹ dry soil; 75 kg ha⁻¹ of basal-dressed nitrogen, which corresponds to 50% of the standard, was optimum when soil nitrate before cultivation was under 100 mg kg⁻¹ dry soil. A standard yield was secured and the rate of nitrogen fertilizer application decreased by 49–76% of the standard by keeping the nitrate concentration of tomato petiole sap between 1000–2000 mg L⁻¹ from early harvest time to topping time under these conditions.  相似文献   

Kinship and inbreeding estimates based on microsatellite markers in breeding of Japanese pear (Pyrus pyrifolia Nakai)     

Noriyuki Onoue  Masahiko Yamada  Toshiya Yamamoto  Shingo Terakami  Chikako Nishitani  Miyuki Kunihisa  Norio Takada  Sogo Nishio  Yutaka Sawamura  Toshihiro Saito 《Euphytica》2015,205(2):539-555

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Age and growth of yellowstriped butterfish, <Emphasis Type="Italic">Labracoglossa argentiventris</Emphasis>, around Izu Oshima Island     

Shingo?Watari  Junji?Yonezawa  Sakutaro?Yamada  Eiji?TanakaEmail author  Toshihide?Kitakado 《Fisheries Science》2005,71(1):86-94

ABSTRACT:   Age and growth of the yellowstriped butterfish, Labracoglossa argentiventris , around Izu Oshima Island were studied using a total of 1450 fish. Age was determined by counting the edge of the opaque zones as a ring mark on sectioned sagittal otoliths. Formation of the first ring was observed during spring or summer, corresponding to 1.5 years after hatching. Thereafter, one ring was formed each year in the same season as the previous year. The growth of the butterfish was rapid until 2 years of age. The maximum likelihood method was applied to the age and length data for estimating parameters in von Bertalanffy, Gompertz, and Logistic growth models. The selected model, based on the Akaike Information Criterion, was the von Bertalanffy growth model, which indicated differential asymptotic length and variance by sex.  相似文献   

Why do young Pacific bluefin tuna repeatedly dive to depths through the thermocline?     

Takashi Kitagawa  Shingo Kimura  Hideaki Nakata  Harumi Yamada 《Fisheries Science》2007,73(1):98-106

Young Pacific bluefin tuna Thunnus orientalis with archival tags were released in the East China Sea. Time-series data for depth, and ambient and peritoneal temperatures for nine fish, recorded every 128 s, were analyzed. Our objectives were to describe monthly changes in diving patterns in relation to the ambient thermal structure and the occurrence of feeding events during March–June, and to discuss possible reasons why bluefin tuna repeatedly dived to depths below the thermocline in terms of their thermoconservation mechanisms. It was found that the fish repeatedly dived through the thermocline at intervals of 1.2 h on average, and the dive frequency was high during March–May. However, the dive frequency and periodicity decreased in June, when the gradient became steeper. In contrast, feeding events increased in June. These results indicate that from March to May, bluefin tuna repeatedly dive because food biomass is inadequate at the surface, and they stop undertaking repeated dives in June when food becomes more readily available at the surface, in addition to low visibility caused by low solar radiation. Further, the range of heat transfer times for these fish was so long that their peritoneal temperature was probably maintained by engaging in brief dives. The periodicity of dives may lead to a lower fluctuation in the peritoneal temperature, suggesting that the dives are a kind of behavioral thermoregulation.  相似文献   

High mobility group box 1 (HMGB1) protein is present in the cerebrospinal fluid of dogs with encephalitis     

Miyasho T  Nakamura K  Nomura S  Kawasako K  Nakade T  Yamada S  Yokota H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(7):917-922

Many cases of encephalitic disease with unknown etiologies have been reported in specific breeds of small dogs. High mobility group box 1 (HMGB1) in neuronal cells was recently found to be a novel cytokine-like mediator that is a marker of neuronal necrosis and inflammation. The aim of this study was to determine whether HMGB1 levels are elevated in the cerebrospinal fluid (CSF) of dogs suspected of having encephalitis. CSF was obtained from 31 dogs that were diagnosed with an encephalitic disease by clinical examinations and magnetic resonance image (MRI) scanning. The CSF samples were analyzed via western blotting (WB) and an enzyme-linked immunosorbent assay (ELISA) with a polyclonal antibody against HMGB1. The mean HMGB1 concentration was significantly higher in the encephalitic dogs than that in the healthy controls. The concentrations of HMGB1 were correlated with the cell counts and total protein concentrations, which are known CSF indicators of the neuronal inflammation associated with encephalitis. These results suggest that HMGB1 protein in CSF confirms the presence of necrosis and inflammation in most cases of canine encephalitis and that HMGB1 will be a new indicator of encephalitis.  相似文献   

TRANSORBITAL ECHOENCEPHALOGRAPHY IN CATTLE     

Takeshi  Tsuka  DVM  Shingo  Okamura  DVM  Munekazu  Nakaichi  DVM  PND  Satoshi  Une  DVM  PHD  Yasuho  Taura  DVM  PHD 《Veterinary radiology & ultrasound》2002,43(1):55-61

The purpose of this study was establishment of the criteria of transorbital echoencephalography in cattle and experimental applications to bovine practice. Quantitative investigations using magnetic resonance (MR) imaging revealed that this examination could be applied to cattle under 3 months of age. The method of transducer positioning was established in Japanese Black (J.B.) and filial (F1) cattle (turning caudally at an angle of about 16 degrees and dorsally at an angle of about 23 degrees) or in Holstein cattle (turning caudally at an angle of about 20 degrees and dorsally at an angle of about 21 degrees). Examinations in clinical calves revealed that the cerebral parenchyma and the lateral ventricle could be detected antemortem or postmortem. In this study, the diagnoses of hydrocephalus or hydranencephaly was possible using antemortem transorbital echoencephalography. Transorbital echoencephalography was especially useful as the imaging method for bovine hydranencephaly.  相似文献   

Both PPARgamma and C/EBPalpha are sufficient to induce transdifferentiation of goat fetal myoblasts into adipocytes     

Yamanouchi K  Ban A  Shibata S  Hosoyama T  Murakami Y  Nishihara M 《The Journal of reproduction and development》2007,53(3):563-572

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Optic pathway degeneration in Japanese black cattle     

Shiori CHIBA  Shingo FUNATO  Noriyuki HORIUCHI  Kotaro MATSUMOTO  Hisashi INOKUMA  Hidefumi FURUOKA  Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):147-154

Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of glial fibrillary acidic protein was observed in the retinal stratum opticum and ganglion cell layer in all 5 cattle in which the optic pathway lesions could be examined. As etiological research, we also examined whether the concentrations of vitamin A and vitamin B12 or bovine viral diarrhea virus (BVDV) infection was associated with optic pathway degeneration. However, our results suggested that the observed optic pathway degeneration was probably not caused by these factors. These facts indicate the presence of optic pathway degeneration characterized by severe gliosis that has never been reported in cattle without bilateral compressive lesions in the optic pathway or bilateral severe retinal atrophy.  相似文献