Caulogenic callus induction and adventitious bud formation from embryos of long-term stored seeds ofPicea jezoensis     

Shinjiro Ogita  Takafumi Kubo  Masami Fushitani 《Journal of Forest Research》1997,2(3):141-145

Caulogenic calli with a high differentiation potency were induced from mature embryos ofPicea jezoensis seeds stored over a long time, for 29 years, resulting in the active formation of adventitious buds. Embryos began to induce calli within 3 weeks of cultivating on LP medium containing 3 μM BAP and 1 μM 2,4-D. Then, the calli proliferated and transformed into caulogenic calli with bud primordia in 8 weeks. The caulogenic calli increased actively with the addition of 500 mg/l ofl-glutamine in the medium. Furthermore, caulogenic calli, induced on LP medium containingl-glutamine, resulted in the formation of adventitious buds, which elongated after transferring the calli into LP medium with 0.1 μM BAP, but withoutl-glutamine. It appears that the number of adventitious buds and the process of shoot elongation are influenced by the kind of nitrogen contained in the medium for callus induction. A part of this study was presented at the 107th Annual Meeting of the Japanese Forestry Society (1996).  相似文献   

Influence of osmotic pressure on somatic embryo maturation in Pinus densiflora     

Mariko Shoji  Hidenori Sato  Remi Nakagawa  Ryo Funada  Takafumi Kubo  Shinjiro Ogita 《Journal of Forest Research》2006,11(6):449-453

The effect of an osmoticum, polyethylene glycol (PEG), on somatic embryo production was examined using embryogenic cells of Pinus densiflora. In the basal medium containing 30 μM abscisic acid and 6% maltose, the quality of the embryos formed was poor even though somatic embryos were produced. The addition of PEG with molecular weight of 4000 or 8000 significantly enhanced the development of both the quality and quantity of somatic embryos. Furthermore, higher levels of a constant osmotic pressure with PEG 8000 in a range from about 300 to 450 mmol/kg could remarkably enhance the morphogenesis of somatic embryos and their number of embryos produced. A higher stable osmotic pressure with an appropriate molecular weight of PEG is a key factor for the production of good quality somatic embryos in P. densiflora.  相似文献   

Characteristics of systemic infection and host responses in chickens experimentally infected with Salmonella enterica serovar Gallinarum biovar Gallinarum     

Shinjiro OJIMA  Masashi OKAMURA  Nana OSAWA  Akiko TAMURA  Kazuki YOSHIOKA  Takashige KASHIMOTO  Takeshi HANEDA  Hisaya K. ONO  Dong-Liang HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1147

Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) is a host-specific pathogen causing systemic infection in poultry, which leads to significant economic losses due to high mortality. However, little is known about the dynamic process of systemic infection and pathogenic characteristics of S. Gallinarum in chickens. In the present study, we developed an oral infection model that reproduces the pathology of S. Gallinarum and clarified the host immune response of the infected chickens. Chickens at 20 days of age orally inoculated at a dose of 10⁸ colony forming unit (CFU) showed typical clinical signs of fowl typhoid and died between 6 and 10 days post infection. The inoculated S. Gallinarum rapidly disseminated to multple organs and the bacterial counts increased in the liver and spleen at 3 days post infection. Pathological changes associated wirh inflammation in the liver and spleen became apparent at 4 days post infection, and increased expression of interferon (IFN)-γ and interleuikin (IL)-12 in the liver and spleen did not observed until 3 days post infection. These results indicate that S. Gallinarum rapidly spread to entire body through intestine, and the low-level of inflammatory responses in the liver during the early stage of infection may contribute to rapid, systemic dissemination of the bacteria. Our infection model and findings will contribute to the better understanding of the pathogenic mechanism of S. Gallinarum, and provide new insights into the prevention and control of fowl typhoid.  相似文献   

Regions of strong coupling between soil moisture and precipitation   总被引：26，自引：0，他引：26  

Koster RD  Dirmeyer PA  Guo Z  Bonan G  Chan E  Cox P  Gordon CT  Kanae S  Kowalczyk E  Lawrence D  Liu P  Lu CH  Malyshev S  McAvaney B  Mitchell K  Mocko D  Oki T  Oleson K  Pitman A  Sud YC  Taylor CM  Verseghy D  Vasic R  Xue Y  Yamada T;GLACE Team 《Science (New York, N.Y.)》2004,305(5687):1138-1140

Previous estimates of land-atmosphere interaction (the impact of soil moisture on precipitation) have been limited by a lack of observational data and by the model dependence of computational estimates. To counter the second limitation, a dozen climate-modeling groups have recently performed the same highly controlled numerical experiment as part of a coordinated comparison project. This allows a multimodel estimation of the regions on Earth where precipitation is affected by soil moisture anomalies during Northern Hemisphere summer. Potential benefits of this estimation may include improved seasonal rainfall forecasts.  相似文献   

An autopsy case of corrosive gastritis caused by calcium polysulfide colloid preparation     

Eriko Ohkubo  Takeshi Kondo  Yasushi Nagasaki 《Journal of toxicologic pathology》2020,33(4):287

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Steroid-binding characteristics of sex hormone-binding globulin (SHBG) in carp, Cyprinus carpio     

M. Okunaga  T. Matsubara  N. Ohkubo  M. Nagae 《Fish physiology and biochemistry》2003,28(1-4):219-220

In this study, the binding characteristics of SHBG were investigated in carp by the combination of FPLC and steroid ELISA techniques. The results of this study suggest that almost all E₂ was bound to SHBG in the blood.  相似文献   

Enzyme-linked immunosorbent assay (ELISA) for detection of IgM and IgG antibodies to Japanese encephalitis virus in sera from experimentally infected swine     

Y Ohkubo  I Takashima  N Hashimoto  I Fujita 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(1):57-64

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Evidence of global chlorophyll d     

Kashiyama Y  Miyashita H  Ohkubo S  Ogawa NO  Chikaraishi Y  Takano Y  Suga H  Toyofuku T  Nomaki H  Kitazato H  Nagata T  Ohkouchi N 《Science (New York, N.Y.)》2008,321(5889):658

Although analyses of chlorophyll d (Chl d)-dominated oxygenic photosystems have been conducted since their discovery 12 years ago, Chl d distribution in the environment and quantitative importance for aquatic photosynthesis remain to be investigated. We analyzed the pigment compositions of surface sediments and detected Chl d and its derivatives from diverse aquatic environments. Our data show that the viable habitat for Chl d-producing phototrophs extends across salinities of 0 to 50 practical salinity units and temperatures of 1 degrees to 40 degrees C, suggesting that Chl d production can be ubiquitously observed in aquatic environments that receive near-infrared light. The relative abundances of Chl d derivatives over that of Chl a derivatives in the studied samples are up to 4%, further suggesting that Chl d-based photosynthesis plays a quantitatively important role in the aquatic photosynthesis.  相似文献   

Determination of neonicotinoid pesticide residues in vegetables and fruits with solid phase extraction and liquid chromatography mass spectrometry   总被引：9，自引：0，他引：9  

Obana H  Okihashi M  Akutsu K  Kitagawa Y  Hori S 《Journal of agricultural and food chemistry》2003,51(9):2501-2505

A rapid and simple extraction method for the simultaneous analysis of five neonicotinoid insecticides has been developed. Twelve different fruit and vegetable matrixes were extracted with methanol and cleaned up using a graphitized carbon solid phase extraction cartridge loading with a 20% methanol solution. The concentrated eluate after methanol elution was then analyzed for pesticide residues by liquid chromatography/mass spectrometry in the APCI positive mode. The five pesticides including nitenpyram, thiamethoxam, imidacloprid, acetamiprid, and thiacloprid were recovered at 70-95% at spike levels of 0.1 and 1 mg/kg in bell pepper, cucumber, eggplant, grape, grapefruit, Japanese radish, peach, pear, potato, rice, and tomato. Relative standard deviations were less than 10% for all of the recovery tests. The proposed method is fast, easy to perform, and could be utilized for regular monitoring of pesticide residues.  相似文献   

Determination of 15N abundance of amino acids in soil hydrolysates     

Shinjiro Kanazawa  Tadakatsu Yoneyama 《Soil Science and Plant Nutrition》2013,59(1):153-155

¹⁵N abundance of amino acids in soil hydrolysates was determined by emission spectroscopic method.  相似文献