Stocking effectiveness of hatchery-released kuruma prawns estimated by two-stage sampling of commercial catch in Ariake Sound, Japan     

Tadanori Yamaguchi  Shiro Ito  Katsuyuki Hamasaki  Shuichi Kitada 《Fisheries Science》2006,72(2):233-238

ABSTRACT:    To evaluate the stocking effectiveness of the kuruma prawn Penaeus japonicus , a two-stage sampling survey of Gensiki bottom-drift-netters was carried out in Ariake Sound, Saga Prefecture, Japan. A total of 471 000 hatchery-produced juveniles, marked by cutting off the right uropods, was released. Thirty-three fishing days were randomly drawn from four boats, which were selected from the 80 boats operating in the fishery. All prawns caught on the selected days were examined, and the hatchery-produced individuals were identified by their regenerated uropods. A telephone survey of all boats was also conducted, to determine the number of fishing days. A total of 286 marked prawns were caught on the survey days for the Saga area in Ariake Sound, from the last half of July to the first half of September 2002. The recapture rate and standard error were estimated at 0.76 ± 0.52%. Economic efficiency was estimated at 0.28 ± 0.02. Strategies for sampling schemes and stock recovery are also discussed.  相似文献   

Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines     

Okumura S  Saitoh H  Ishikawa T  Wasano N  Yamashita S  Kusumoto K  Akao T  Mizuki E  Ohba M  Inouye K 《Journal of agricultural and food chemistry》2005,53(16):6313-6318

Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL.  相似文献   

Effects of growth and dietary crude protein level until first insemination on milk production during first lactation in Holstein heifers     

Takashige ISHII  Kenji KAWASHIMA  Haruo ORIBE  Hiromi UEDA  Toshiya HASUNUMA  Kiyoshi AKIYAMA  Hirofumi NAKAYAMA  Mitsunori KURIHARA  Fuminori TERADA  Shiro KUSHIBIKI 《Animal Science Journal》2011,82(6):741-746

To decrease the age at first calving in Holsteins, the effects of average daily body weight gain (ADG) and crude protein (CP) level until first insemination on growth performance and milk production were examined. The MM group had a target ADG of 0.75 kg and received a diet with a CP level of 14%. The HM and HH groups had a target ADG of 1 kg; both these groups received a diet with CP levels 14% and 16%, respectively. The ADG in the HM and HH groups was 1.1 kg, whereas in the MM group it was 0.97 kg (P < 0.01). The HM and HH groups showed no differences in withers height at body weight 350 kg. The ages at first calving in MM, HM and HH groups were 23.1, 21.0 and 21.8 months, respectively. The HM and HH groups had lower milk yield at day 305 than the MM group (P < 0.01). These results suggest that growth performance until first insemination should be maintained at an ADG of 0.97 kg or less with a CP level of approximately 14%, to shorten time until first insemination and prevent the decrease of milk yield.  相似文献   

Comparison of Ni/Zn Accumulation Ability of Thlaspi japonicum from Three Different Areas in Hokkaido     

Takafumi Mizuno  Hitoshi Obata  Kenji Horie  Shiro Nosaka  Naoharu Mizuno 《Soil Science and Plant Nutrition》2005,51(4):589-594

Thlaspi japonicum is a Japanese Ni-hyperaccumulator plant growing in areas with serpentine soil containing a high level of Ni. In the present study, we investigated the ability of wild Thlaspi japonicum plants inhabiting non-ultramafic soil areas of Hokkaido to accumulate Ni/Zn. Wild T. japonicum plants growing on Rebun Island (Rebun-T) accumulated small amounts of Ni, while the plants cultivated on ultramafic rock soil accumulated large amounts of Ni, like T. japonicum growing on Mt. Yubari (Yubari-T), and this change was also observed for T. japonicum growing on Mt. Tengu (Tengu-T). All the T. japonicum plants transplanted on both ultramaflc soil or Andosol accumulated over 100 mg kg⁻¹ Zn, especially the Ni/Zn content of Tengu-T plants tended to be higher than that of the plants growing in the other two areas. In contrast, transplanting of Yubari-T plants from ultramafic soil with a high level of Ni to soil with a lower-Ni content decrease of the Ni concentration in the leaves. Wild Rebun-T plants showed a smaller biomass than the Yubari-T and Tengu-T plants, and some individuals of the Rebun-T population were smaller than those of the other populations, with tiny radical leaves. Zinc content of the small leaves was not significantly different from that in large leaves. These results suggested that T. japonicum plants are able to accumulate high Ni/Zn accumulation, regardless ecotype, habitat and leaf size.  相似文献   

Identification of geranic acid, a tyrosinase inhibitor in lemongrass (Cymbopogon citratus)     

Masuda T  Odaka Y  Ogawa N  Nakamoto K  Kuninaga H 《Journal of agricultural and food chemistry》2008,56(2):597-601

Lemongrass is a popular Asian herb having a lemon-like flavor. Very recently, potent tyrosinase inhibitory activity has been found in lemongrass in addition to various biological activities reported in the literature. The aim of the present study is to identify the active compounds in the lemongrass. An assay-guided purification revealed that one of the active substances was geranic acid. Geranic acid has two stereoisomers, which are responsible for the trans and cis geometry on the conjugated double bond. Both isomers are present in the active ethyl acetate-soluble extract of the lemongrass, and their IC50 values were calculated to be 0.14 and 2.3 mM, respectively. The structure requirement of geranic acid for the potent tyrosinase inhibitory activity was investigated using geranic acid-related compounds.  相似文献   

Comparative analysis of organelle DNAs in acid citrus grown in Japan using PCR-RFLP method   总被引：1，自引：0，他引：1  

Asad Asadi Abkenar  Shiro Isshiki  Ryoji Matsumoto  Yosuke Tashiro 《Genetic Resources and Crop Evolution》2008,55(4):487-492

PCR-RFLP analyses of three regions for each of chloroplast DNA (cpDNA; rbcL-ORF106, trnD-trnT, trnH-trnK) and mitochondrial DNA (mtDNA; nad7/exon2-exon3, nad7/exon3-exon4, 18S-5S) were performed in 26 cultivars of acid citrus grown in Japan to identify polymorphisms and classify them. The polymorphisms were compared with those of three true Citrus species, i.e., mandarin, pummelo and citron. Ichang papeda (C. ichangensis) was also included in this study to find its relationship with Yuzu. Inter-species cpDNA variation was recognized and the acid citrus were divided into three groups, namely; I (‘Yuzukichi’ and ‘Kinkoyu’), II [sour oranges (‘Kaiseito’, ‘Daidai’ and ‘China daidai’), ‘Nansho daidai’, ‘Kiku daidai’, C. sudachi (‘Mushi yukaku’, ‘Yushi yukaku’ and ‘Yushi mukaku’), C. sphaerocarpa (‘Kabosu’ and ‘Aka kabosu’), C. kizu (‘Taninaka kizu’, ‘Kinosu’ and ‘Kizu’), ‘Zanbo’, ‘Mochiyu’, ‘Jabara’ and ‘Naoshichi’], and III [Yuzu (‘Tetraploid’, ‘Tochikei yuzu’ and ‘Yamanekei yuzu’), ‘Matsuda sudachi’, ‘Zuishoyu’, ‘Hanayu’ and ‘Yuko’]. CpDNA restriction patterns of the three true Citrus species differed from each other as well as from those of ichang papeda. CpDNA restriction patterns of group I of the acid citrus were identical to those of mandarins. Group II showed the same as pummelos. CpDNA restriction patterns of group III were differed from those of the three true Citrus species in the three regions. This group was differed from ichang papeda after digestion of trnH-trnK PCR products with TaqI, HinfI and AluI, while they showed identical restriction patterns in two regions, rbcL-ORF106 and trnD-trnT. Citrons and ichang papeda were placed in groups IV and V, respectively. Based on mtDNA restriction patterns, the acid citrus were divided into three groups; i, ii and iii. In groups i and ii accessions of groups I and II of cpDNA were placed with mandarins and pummelos, respectively. In group iii accessions of group III of cpDNA were placed with ichang papeda. Citrons were placed in a distinct group, iv.  相似文献   

用加压热水预处理山毛榉树粉生产燃料乙醇的初步研究     

吕欣  坂志朗  董明盛  陈晓红  姜梅 《农业工程学报》2008,24(3):219-222

采用间歇式加压热水实验装置,研究了加压热水用于山毛榉树粉的分离规律,探讨了不同温度、处理时间对生物质分离的影响.当温度低于200℃时 ,随着温度升高和反应时间延长,木粉溶解量、木糖浓度和糠醛浓度均逐渐升高,而葡萄糖浓度和5-羟甲基糠醛浓度则较低.但当温度高于200 ℃时,生物质溶解量基本不随反应时间的变化而变化,葡萄糖浓度随反应时间延长逐渐增加,木糖浓度则随着反应时间的延长而下降,糠醛浓度则随着反应时间的延长基本不变甚至略有下降,5-羟甲基糠醛浓度则随着反应时间的延长而增大.  相似文献   

Effect of feeding wood kraft pulp on the growth performance,feed digestibility,blood components,and rumen fermentation in Japanese Black fattening steers     

Yuka Maeda  Keiko Nishimura  Kazuhiro Kurosu  Hitoshi Mizuguchi  Shigeru Sato  Fuminori Terada  Shiro Kushibiki 《Animal Science Journal》2019,90(4):523-532

This study aimed to examine the effects of feeding kraft pulp (KP) on the growth performance, feed digestibility, and rumen fermentation of Japanese Black fattening steers. Ten Japanese Black fattening steers (aged 26 months) were randomly divided into control and KP groups. The control group (n = 5) was fed concentrate feed without KP, and the KP group (n = 5) was fed concentrate feed containing 10% KP. Both the groups were provided rice straw as roughage. The experiment was conducted over a period of 12 weeks. There was no significant difference in dry matter intake, daily body weight gain, and nutrient digestibility between both groups. No difference was observed in the ruminal concentrations of volatile fatty acids among the groups. At weeks 8 and 12 after the onset of the experiment, the acetate‐to‐propionate ratio in the ruminal fluid of the KP group was significantly higher than that of the control group. The average daily pH of ruminal fluid and activity of ruminal lipopolysaccharide did not differ between the groups. Our results suggested that the growth performance and feed digestibility in the Japanese Black fattening steers were not influenced by replacing concentrate feed with KP.  相似文献   

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method          下载免费PDF全文

Tao Zhuang  Megumi Urakawa  Hidetoshi Sato  Yuko Sato  Teruaki Taguchi  Tsuyoshi Umino  Shiro Katto  Koutaro Tanaka  Kozue Yoshimura  Naokazu Takada  Hiroko Kobayashi  Megumi Ito  Michael T. Rose  Yoshio Kiku  Yuya Nagasawa  Haruki Kitazawa  Kouichi Watanabe  Tomonori Nochi  Tomohito Hayashi  Hisashi Aso 《Animal Science Journal》2018,89(7):1011-1019

Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a⁺/CD11c⁺/MHC (major histocompatibility complex) class II⁺ cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a⁺/CD11c⁺ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.  相似文献   

Aerodynamic sound and self-excited vibration of circular saw with step thickness I: Comparison of dynamic characteristics between the common circular saw and the circular saw with step thickness     

Wuhua Cheng  Hideyuki Yokochi  Shiro Kimura 《Journal of Wood Science》1998,44(3):177-185

The natural frequencies, aerodynamic noise, whistling noise, and self-excited vibration between the common circular saw with uniform thickness and the circular saw with step thickness (thin on its outer part and thick on its inner part) were researched during idling. The natural frequencies of the circular saw with step thickness increased with an increase in the thickness and in the radius of the thick part. The frequencies of the circular saws with step thickness (thick at the inner part) were higher than that of the thin circular saw with a uniform thickness of 0.5mm. The aerodynamic sound pressure level induced from circular saw teeth increased with an increase in the tooth thickness. The whistling noise and self-excited vibration easily occurred in the thick circular saws (1.5, 2.0, and 2.5mm). The whistling sound pressure level and the vibration amplitude increased with an increase in the thickness of the saw. None of the designed circular saws with step thickness generated whistling noises or self-excited vibration during idling.Part of this report was presented at the 45th annual meeting of the Japan Wood Research Society at Tokyo, April 1995 and the 12th International Wood Machining Seminar at Kyoto, October 1995  相似文献