Valine pathway is more crucial than phenyl propanoid pathway in regulating capsaicin biosynthesis in Capsicum frutescens mill     

Prasad BC  Gururaj HB  Kumar V  Giridhar P  Ravishankar GA 《Journal of agricultural and food chemistry》2006,54(18):6660-6666

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Genetic analysis of yield and quantitative traits in pigeonpea (<Emphasis Type="Italic">Cajanus cajan</Emphasis> L. Millsp.)     

B. C. Ajay  B. N. Gnanesh  K. N. Ganapathy  M. Byre Gowda  P. S. Prasad  G. N. Veerakumar  S. C. Venkatesha  R. Abdul Fiyaz  K. T. Ramya 《Euphytica》2012,186(3):705-714

Basic information on genetics and inheritance of quantitative characters, which is necessary to develop future breeding programme, is not widely studied in pigeonpea. Hence, present study was conducted among 5 generations in four pigeonpea crosses to know significance of additive-dominance model, gene action involved in inheritance of quantitative characters, heritability and genetic advance. “Scaling” and “joint scaling test” was significant for most characters indicating that additive-dominance model alone is not enough to explain the inheritance of a character. Though additive variance was more, dominance variance also played important role for most of the traits. Positive and negative alleles were found to be distributed between parents. Additive gene effect (d) was significant for pods per plant and seeds per pod whereas dominance gene effect (h) was more predominant among pod yield and seed yield. Dominance × Dominance inter-allelic interactions (l) was more important than Additive × Additive type (i) for most of the traits studied which could be exploited by selecting individuals based on their performance in recurrent selection. Complementary gene action was observed among many traits with few exhibiting duplicate gene action. Heritability and genetic advance was high indicating the effectiveness of selection. Since dominance effects is also present along with additive effects selection could be practised in later generations to identify high yielding genotypes.  相似文献   

Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I   总被引：1，自引：0，他引：1  

Starck SR  Jiang V  Pavon-Eternod M  Prasad S  McCarthy B  Pan T  Shastri N 《Science (New York, N.Y.)》2012,336(6089):1719-1723

Effective immune surveillance by cytotoxic T cells requires newly synthesized polypeptides for presentation by major histocompatibility complex (MHC) class I molecules. These polypeptides are produced not only from conventional AUG-initiated, but also from cryptic non-AUG-initiated, reading frames by distinct translational mechanisms. Biochemical analysis of ribosomal initiation complexes at CUG versus AUG initiation codons revealed that cells use an elongator leucine-bound transfer RNA (Leu-tRNA) to initiate translation at cryptic CUG start codons. CUG/Leu-tRNA initiation was independent of the canonical initiator tRNA (AUG/Met-tRNA(i)(Met)) pathway but required expression of eukaryotic initiation factor 2A. Thus, a tRNA-based translation initiation mechanism allows non-AUG-initiated protein synthesis and supplies peptides for presentation by MHC class I molecules.  相似文献   

Conservation of microsatellite regions across legume genera enhances marker repertoire and genetic diversity study in Phaseolus genotypes     

Subhojit Datta  Mayank Kaashyap  Pallavi Singh  P. Gupta  Tuba Anjum K.  Sahil Mahfooz  Sanjeev Gupta 《Plant Breeding》2012,131(2):307-311

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Genomic characterization of drought tolerance-related traits in spring wheat     

Sundeep Kumar  Sunish Kumar Sehgal  Uttam Kumar  P. V. Vara Prasad  Arun Kumar Joshi  Bikram Singh Gill 《Euphytica》2012,186(1):265-276

Drought tolerance was investigated in ‘C306’, one of the most drought tolerant wheat cultivars bred in India in the 1960’s. An intervarietal mapping population of recombinant inbred lines of the cross ‘C306’ × ‘HUW206’ was evaluated for drought tolerance components, namely potential quantum efficiency of photosystem (PS) II (F_v/F_m), chlorophyll content (Chl), flag leaf temperature (Lt), and grain yield per plant (Gyp) under stress. Three independent experiments were conducted under well-watered and water-stressed conditions in greenhouses and growth chambers at Kansas State University (USA). Five hundred and sixty microsatellite markers covering the entire genome were screened for polymorphism between the parents. A QTL (QLt.ksu-1D) for Lt (low flag leaf temperature under stress) on the short arm of chromosome 1D between markers Xbarc271 and Xgwm337 at LOD 3.5 explained 37% of the phenotypic variation. A QTL for F_v/F_m (QF _v /F _m .ksu-3B) and Chl (QChl.ksu-3B) controlling quantum efficiency of PS II and chlorophyll content under stress were co-localized on chromosome 3B in the marker interval Xbarc68–Xbarc101 and explained 35–40% of the phenotypic variation for each trait. A QTL (QGyp.ksu-4A) for Gyp on chromosome 4A at a LOD value of 3.2 explained 16.3% of the phenotypic variation. Inconsistent QTLs were observed for F_v/F_m on chromosomes 3A, 6A, 2B, 4B, and 4D; for Chl on 3A, 6A, 2B and 4B; and for Lt on 1A, 3A 6A, 3B and 5B. The identified QTLs give a first glimpse of the genetics of drought tolerance in C306 and need to be validated in field experiments using the marker-phenotype linkages reported here.  相似文献   

Characterisation and validation of housekeeping genes for qRT-PCR expression analysis in Pterophyllum scalare     

Sushila  Ngairangbam  Das  Basanta Kumar  Prasad  K. Pani  Tripathi  Gayatri 《Aquaculture International》2021,29(6):2387-2402

Aquaculture International - Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive and advanced method to quantify the expression of target genes of animals including fish. However, the...  相似文献   

Differentiation of infectious bursal disease viruses by restriction enzyme analysis of RT-PCR amplified VP1 gene sequence     

Tiwari AK  Kataria RS  Indervesh  Prasad N  Gupta R 《Comparative immunology, microbiology and infectious diseases》2003,26(1):47-53

In order to differentiate infectious bursal disease virus (IBDV) isolates/strains, a quick method of RT-PCR followed by restriction enzyme analysis of VP1 gene sequence is being reported for the first time. A 480 bp fragment, comprising one of the RNA dependent RNA polymerase motifs of VP1 gene sequence of an Indian classical virus, an attenuated vaccine strain, Georgia and two Indian field isolates, genetically similar to reported very virulent strains of IBDV, was amplified by RT-PCR. Restriction enzyme digestion of PCR products with Taq1 enzyme generated distinct profile for field isolates, different from the classical and attenuated viruses, whereas restriction profile with BstNI restriction enzyme was similar in all the viruses, irrespective of the pathotype. Therefore, the present results suggest that Taq1 digestion can be taken up for the differentiation of field isolates from the classical and vaccine strains. The sequence analysis of VPI gene of reported very virulent IBD viruses from Europe and Japan, using 'MapDraw' programme of Lasergene software, revealed similar restriction enzyme profile as in Indian field isolates.  相似文献   

Turkey herpesvirus and Marek's disease virus. A comparative appraisal.     

L B Prasad 《Comparative immunology, microbiology and infectious diseases》1979,2(2-3):335-358

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Efficacy of disinfectants and hand sanitizers against avian respiratory viruses     

Patnayak DP  Prasad AM  Malik YS  Ramakrishnan MA  Goyal SM 《Avian diseases》2008,52(2):199-202

Disinfectants play a major role in the control of animal diseases by decontaminating the farm environment. We evaluated the virucidal efficacy of nine commonly used disinfectants on a nonporous surface contaminated experimentally with avian metapneumovirus (aMPV), avian influenza virus, or Newcastle disease virus (NDV). Phenolic compounds and glutaraldehyde were found to be the most effective against all three viruses. Quaternary ammonium compounds were effective against aMPV but not against the other two viruses. In addition, efficacy of commercially available hand sanitizers was evaluated on human fingers contaminated with aMPV and NDV. All three hand sanitizers tested were found to be effective against both viruses within 1 min of application on fingers.  相似文献   

Influence of Pre-Drying Treatments on Physico-Chemical and Phytochemical Potential of Dried mahua Flowers     

Pinakin  Dave Jaydeep  Kumar  Vikas  Kumar  Satish  Kaur  Sawinder  Prasad  Rasane  Sharma  Basista Rabina 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):576-582

Plant Foods for Human Nutrition - Mahua (Madhuca longifolia) flowers are boon for the local tribes of India and have been successfully utilized traditionally for the value addition, and are very...  相似文献