Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel,Anguilla japonica,at Completion of Yolk Resorption     

Tadahide Kurokawa  Takuma Okamoto  Koichiro Gen  Susumu Uji  Koji Murashita  Tatsuya Unuma  Kazuharu Nomura  Hajime Matsubara  Shin‐Kwon Kim  Hiromi Ohta  Hideki Tanaka 《Journal of the World Aquaculture Society》2008,39(6):726-735

The occurrence of morphological deformities under different rearing water temperatures (18, 20, 22, 24, 26, 28, and 30 C) was examined in Japanese eel larvae. The rates of hatching and survival until yolk resorption at 22–26 C were higher than those at other water temperatures. Fertilized eggs never hatched at 18 and 30 C. The rates of occurrence of abnormal larvae reared at the water temperatures 24–28 C were lower than those at 20 or 22 C. Pericardial edema and lower jaw deformities occurred most frequently at lower temperatures (20 and 22 C). In contrast, the incubation temperature did not significantly affect the relative frequency of some neurocranial deformities and of spinal curvature. These results imply that the optimal temperatures for rearing Japanese eel eggs and embryos are 24–26 C from the viewpoints of survival and deformity.  相似文献   

Current status of genetic transformation technology developed in cucumber (Cucumis sativus L.)     

WANG Shun-li  Seong Sub Ku  YE Xing-guo  HE Cong-fen  Suk Yoon Kwon  Pil Son Choi 《中国农业科学(英文版)》2015,(3):469-482

Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.  相似文献   

假单胞菌中抗真菌成分对植物病原真菌准性产孢的抑制作用     

Yoon.k.s Rew.H.R 《安徽农业大学学报》1999,26(3):252-260

我们目前正进行铜绿假单胞菌（PAFS）中抗真菌物质的纯化研究,本文考察对卵菌产孢及菌丝生长具抑制活性的部分纯化成分的特性。PAFS中的初级成分：A1,A2,A3及A6似乎包含2类抗菌组分,A1,A2及A3对子囊菌准性产孢具特异性抑制作用,导致孢子发育不完全,然而有趣的是这些成分对菌丝生长抑制作用不明显。相反,A6对卵菌菌丝生长具抑制活性,另外在含A6条件下产生的孢子萌发的非对称性说明其抗菌活性具有持效性。  相似文献   

Heterosporis anguillarum infections in farm cultured eels (Anguilla japonica) in Korea     

Seong Joon Joh  Yong Kuk Kwon  Min Chul Kim  Min Jeong Kim  Hyuk Man Kwon  Jung Won Park  Jun Hun Kwon  Jae Hong Kim 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(2):147-149

Ten eels (Anguilla japonica) from a fish farm in Korea were examined and diagnosed with a Heterosporis infection. The gross lesions on the trunk were uneven and the concave parts were pasty. Histopathologically, lyses of the trunk muscles, degenerative muscle fibers and the scattered spores were observed. The sporophorocyst (SPC) contained several spores with a variety of shapes. Some SPC were disrupted and the spores in the SPC were scattered in the muscle tissues. Macrophages existed near the scattered spores. Electron microscopy revealed special structures such as sporophorocyst containing various developmental parasitic stages such as meronts, sporonts, sporophorous vesicles and spores.  相似文献   

Application of nucleotide sequence of RNA polymerase beta-subunit gene (rpoB) to molecular differentiation of serovars of Salmonella enterica subsp. enterica.     

H J Kwon  K Y Park  S J Kim  H S Yoo 《Veterinary microbiology》2001,82(2):121-129

To establish a molecular differentiation method for Salmonella enterica subsp. enterica, a hyper-variable region of RNA polymerase beta-subunit (rpoB) of S. enterica subsp. enterica (I), serotype Typhimurium, and Escherichia coli were investigated through comparison of nucleotide sequence of the region. The hyper-variable region was identified at 612-937 of the gene. After PCR amplification of the region in the 17 serotypes and two biotypes of serotype Gallinarum of S. enterica subsp. enterica (I), the nucleotide sequences of the region were determined and compared. All serotypes were distantly related to E. coli with 82.8-84.7% identities in nucleotide sequence while showing 96.6-100% identities with each other. According to the phylogenetic analysis based on the sequenced region with the neighbor-joining method, relatedness of biotype Gallinarum to serotype Enteritidis and biotype Pullorum was determined. Biotype Gallinarum was more closely related to serotype Enteritidis than biotype Pullorum. These results suggested that the 612-937 variable region of rpoB might be useful for molecular evolutionary analysis of serotypes of S. enterica subsp. enterica (I).  相似文献   

Mammary gland adenocarcinoma in a mandrill (Mandrillus sphinix).     

J H Choi  B I Yoon  J H Kim  N S Shin  S W Kwon  K W Lee  D Y Kim 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(11):1233-1235

A 22-year-old female mandrill (Mandrillus sphinix) with continuously growing mass at the right mammary gland area was found dead, and a postmortem examination was performed. At necropsy, an elevated firm subcutaneous mass about 5 cm in diameter was present at the right mammary gland area. Axillary, mediastinal, and tracheobronchial lymph nodes were enlarged 2 to 4 times their normal sizes. Numerous metastatic foci 2 to 5 mm in diameter were scattered in the lung. Histologically, the tumor was diagnosed as mammary gland adenocarcinoma. Metastasis to the regional lymph nodes and lung was also confirmed. This is the first reported case of a mammary gland tumor in mandrill in Asia.  相似文献   

Antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolated from pigs in Korea using new standardized procedures     

Kim B  Min K  Choi C  Cho WS  Cheon DS  Kwon D  Kim J  Chae C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(3):341-342

The in vitro susceptibilities of 76 isolates of Actinobacillus pleuropneumoniae collected from pigs with pleuropneumonia were tested with 12 commonly used antimicrobial drugs by an agar dilution minimal inhibitory concentration procedure according to National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Field isolates had low MICs for ceftiofur, danofloxacin and penicillin. No correlation of antimicrobial resistance was related to serotype.  相似文献   

Detection and localization of ApxI, -II and -III genes of Actinobacillus pleuropneumoniae in natural porcine pleuropneumonia in natural porcine pleuropneumonia by in situ hybridization     

Choi C  Kwon D  Min K  Chae C 《Veterinary pathology》2001,38(4):390-395

In situ hybridization techniques that employed a nonradioactive digoxigenin-labeled probe were used to detect and localize ApxI, II and III genes in tissue sections of pneumonic lung naturally infected with Actinobacillus pleuropneumoniae. In pigs infected with either serotype 2 or 6, a hybridization signal for apxIICA, apxIIICA, apxIBD, and apxIIIBD was detected, and in pigs infected with serotype 5, a hybridization signal for apxICA, apxIICA, and apxIBD was detected in the pneumonic lesions. A hybridization signal for apxIICA and apxIBD was detected in pigs infected with serotype 7. A strong hybridization signal for apx genes was seen in streaming degenerate alveolar leukocytes bordering zones of coagulative necrosis. Simultaneous detection of hybridization signals for the apxCA and apxBD genes provided scientific evidence that the expression of the apx genes could be potential indicators of the production of corresponding Apx toxins. This study demonstrates the expression of ApxI, II, and III genes in pneumonic lesions caused by A. pleuropneumoniae.  相似文献   

Suppression of cytokine-inducible nitric oxide synthesis during intraperitoneal meth a tumor growth     

Kwon OD  Yim CY  Jeong KS  Jung KY  McGregor JR  Bastian NR  Samlowski WE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):357-365

Nitric oxide (NO*) synthesis is induced within many tumors. The timecourse of NO* synthesis was evaluated during intraperitoneal Meth A fibrosarcoma progression. While increasing macrophage recruitment into ascites was noted, inducible nitric oxide synthase (iNOS) antigen and function peaked between days 3-6 after tumor implantation. The capacity of cells to respond to LPS and IFNgamma stimulation was markedly depressed on day 9 and 11. Cellular proliferation correlated in an inverse fashion with levels of NO* synthesis. Electron paramagnetic resonance spectroscopy and nitrotyrosine immunostaining failed to show accumulation of characteristic target cell lesions induced by NO*. These findings lead us to conclude that NO* production was increasingly suppressed during Meth A tumor progression. Depression of NO* production did not correlate with levels of the inhibitory cytokines TGFbeta and IL-10, but could be partially overcome by addition of sepiapterin (a tetrahydrobiopterin prodrug). Thus, depletion of essential co-factors necessary for iNOS function may contribute to depressed NO* responses during cancer progression.  相似文献   

Calcinosis circumscripta on lingual muscles and dermis in a dog     

Jeong W  Noh D  Kwon OD  Williams BH  Park SC  Lee M  Do S  Chung J  Lee G  Yun H  Jeong KS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):433-435

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