Rabbits first-then humans     

Stein G 《Science (New York, N.Y.)》1967,156(3774):457

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Nonhistone chromosomal protein synthesis: utilization of preexisting and newly transcribed messenger RNA's   总被引：3，自引：0，他引：3  

G S Stein  D E Matthews 《Science (New York, N.Y.)》1973,181(94):71-73

Treatment of HeLa S(3) cells with actinomycin D during mitosis suppresses the synthesis of several major classes of nonhistone chromosomal proteins during the subsequent period before DNA replication, but allows the synthesis of other species of these proteins. Such results are consistent with the utilization of preexisting, as well as newly transcribed, messenger RNA's for nonhistone chromosomal protein synthesis during the prereplicative phase of the cell cycle.  相似文献   

Reaction of hydrated electrons with ferricytochrome C     

Lichtin NN  Shafferman A  Stein G 《Science (New York, N.Y.)》1973,179(4074):680-682

The reaction of ferricytochrome c with hydrated electrons produced at pH 6.8 by radiolysis with electron pulses lasting 50 to 1000 nanoseconds has a specific rate constant of 5.5 x 10(10) liter mole(-1) sec(-1) for the formation of the primary adduct. By using appropriate wavelengths, another fast, consecutive process was demonstrated, with the pure first order rate constant 1.0 x 10(5) sec(-1). Its characteristics agree with it being an intramolecular process within the enzyme. Approximately 50 percent of all electrons which add to ferricytochrome c end in forming ferrocytochrome c.  相似文献   

Geochemistry. How old is planet Earth?     

Jacobsen SB;Department of Earth  Planetary Sciences  Harvard University  Cambridge  MA  USA. jacobsen@neodymium.harvard.edu 《Science (New York, N.Y.)》2003,300(5625):1513-1514

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Reactions of aromatic and sulfur amino acids in ribonuclease with hydrogen atoms in water solution     

R Shapira  G Stein 《Science (New York, N.Y.)》1968,162(861):1489-1491

Neutral hydrogen atoms are simple, one-electron-equivalent reagents and their reactions are of fundamental interest for chemical kinetics. Functional correlation between aromatic and divalent-sulfur amino acids in ribonuclease is shown in the reaction of ribonuclease with hydrogen atoms in aqueous solution. Products of the reaction are partially characterized.  相似文献   

Correlation of changes in gravity, elevation, and strain in southern california     

Jachens RC  Thatcher W  Roberts CW  Stein RS 《Science (New York, N.Y.)》1983,219(4589):1215-1217

Measurements made once or twice a year from 1977 through 1982 show large correlated changes in gravity, elevation, and strain in several southern California networks. Precise gravity surveys indicate changes of as much as 25 microgals between surveys 6 months apart. Repeated surveys show that annual elevation changes as large as 100 millimeters occur along baselines 40 to 100 kilometers long. Laser-ranging surveys reveal coherent changes in areal strain of 1 to 2 parts per million occurred over much of southern California during 1978 and 1979. Although the precision of these measuring systems has been questioned, the rather good agreement among them suggests that the observed changes reflect true crustal deformation.  相似文献   

Metabolic precursors of a known human carcinogen     

Moore RM  Woolf BS  Stein HP  Thomas AW  Finklea JF 《Science (New York, N.Y.)》1977,195(4276):344

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Gel filtration and disc gel electrophoresis of tomato pectic substances.     

E R Stein  H E Brown 《Journal of agricultural and food chemistry》1975,23(3):526-529

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Chlorinated compounds and phenols in tissue, fat, and blood from rats fed industrial waste site soil extract: methods and analysis     

V B Stein  R S Narang 《Journal of the Association of Official Analytical Chemists》1984,67(1):111-116

A method was developed to analyze rat tissue, fat, and blood for some of the chlorinated compounds found in an extract of soil from an industrial waste site. Extraction with hexane and then with ethyl ether-hexane (1 + 1) was followed by concentration over steam, and gas chromatographic analysis with an electron capture detector. Volatile compounds were analyzed in a glass column coated with 6% SP-2100 plus 4% OV-11 on Chromosorb W. Semivolatile compounds, chlorinated compounds, and pesticides were analyzed in a 70 m glass capillary column coated with 5% OV-101. Phenols were analyzed in a glass column packed with 1% SP-1240 DA on Supelcoport. However, the most efficient means of separation was to use the same glass column for volatile compounds, a DB-5 fused silica capillary column for semivolatile compounds, pesticides, and phenols, and the same 1% SP-1240 DA glass column for separation of beta-BHC and pentachlorophenol. Recoveries ranged from 86.3 +/- 9.1% (mean +/- standard deviation) to 105 +/- 10.4%. Sensitivities for semivolatile chlorinated compounds, pesticides, and phenols were about 4 ng/g for fat, 1 ng/g for tissue, and 0.2 ng/mL for blood. Sensitivities for volatile compounds were about 4-fold higher (16, 4, and 0.8, respectively). Sensitivities for dichlorobenzenes and dichlorotoluenes were 8 ng/g for fat, 2 ng/g for tissue, and 0.4 ng/mL for blood.  相似文献   

Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers     

Carlos A. Cairo  Juliana Stein  Luciana Delgado  Santiago Bortolotti  Sebastián A. Guelman  Juan Pablo A. Ortiz  Eligio N. Morandi 《Euphytica》2002,124(3):387-395

Soybean cultivars carrying the `long juvenile trait' show a delayed flowering response under short day conditions. The incorporation of this character into genotypes of agronomic interest may allow a broader range of sowing dates and latitudes for a single cultivar adaptation. The objective of this work was to identify molecular markers linked to the juvenile locus in soybean. Experiments were carried out using two pairs of near isogenic lines(NILs) differing in the presence of the long juvenile trait, and RAPD markers. Four hundred primers were first screened to find polymorphism associated with the trait. Additional differences between NILs were sought by digesting the genomic DNA with five restriction enzymes. Polymorphic fragments detected between NILs were tested for linkage to the juvenile locus in the corresponding F₂ segregating populations. Marker bc357-HaeIII was linked (χ²L = 46.316) to the juvenile locus with an estimated recombination frequency of 0.13 ± 0.03in one of the genetic backgrounds studied. The fragment was cloned, sequenced and converted into a SCAR marker. Moreover,bc357-HaeIII was used as RFLP probe. Both, SCAR and RFLP generated markers linked to the juvenile locus in the two genetic backgrounds analysed. Results presented in this work can be utilised for both, the localisation of the gene associated with the character and for tagging the juvenile trait in soybean breeding programs. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献