Integration of soil solarization and potential native antagonist for the management of crown gall on cherry rootstock colt     

A.K. Gupta  K. Khosla 《Scientia Horticulturae》2007

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Association of induction of protease and chitinase in chickpea roots with resistance to Fusarium oxysporum f.sp. ciceri     

Giri  Harsulkar  Patankar  Gupta  Sainani  Deshpande  & Ranjekar 《Plant pathology》1998,47(6):693-699

To improve the breeding of chickpea varieties with resistance to Fusarium wilt, an attempt was made to analyse the biochemical basis of disease resistance by measuring levels of chitinase, β-1,3-glucanase, protease and proteinase inhibitor activities in dry and soaked seeds and in root and shoot tissues of wilt-resistant and wilt-susceptible cultivars. Marginal variation was observed in the levels of the candidate proteins in dry or soaked seeds. Chitinase activity was higher in roots than in shoots or cotyledons. No proteinase inhibitor activity was detected in root and shoot tissue of any of the cultivars. When the levels of these proteins were analysed in resistant (Vijay) and susceptible (JG-62) cultivars during development of wilt by growing plants in soil infested with F. oxysporum f.sp. ciceri , race 1, both cultivars showed induction of chitinase activity in the roots. However, the induced activity in JG-62 (3.82 U g⁻¹) was equivalent to the constitutive level in Vijay (3.90 U g⁻¹) and much lower than that induced in Vijay (5.18 U g⁻¹). Induction of protease activity was observed only in root extracts of Vijay when challenged by the pathogen. The root extract of Vijay showed in vitro antifungal activity in a plate assay. Simultaneous induction of proteolytic and chitinolytic activities specifically in the resistant cultivar was correlated with antifungal properties of root extracts effective in conferring resistance.  相似文献   

Kinetics of Interferon-Gamma Production and its Comparison with Anti-Listeriolysin O Detection in Experimental Bovine Listeriosis     

Barbuddhe SB  Malik SV  Choudhary SP  Gupta LK 《Veterinary research communications》1998,22(8):505-516

The kinetics of the production of interferon gamma (IFN-) in whole blood culture and its comparison with anti-listeriolysin O (ALLO) detection by ELISA were studied during oral infection of calves with Listeria monocytogenes. Culture filtrate antigen (CFA), listeriolysin O (LLO), and sonicated antigen (SA) were used to prime the peripheral blood mononuclear cells (PBMCs) and the plasma from orally infected calves. IFN- and ALLO appeared as early as day 7 of an oral infection. IFN- was detected earlier with LLO than with SA. The Max50 interleukin (IL-2) activity and IFN- estimated in the culture supernatant from PBMCs primed in vitro with different antigens of L. monocytogenes revealed high induction of IL-2 and IFN- by CFA, LLO and live antigen. IFN- assay and ALLO detection were used for testing cases of repeat breeding in dairy cattle. It appeared that detection of IFN- employing LLO can be used to diagnose listerial infections.  相似文献   

Evaluation of cell-mediated immunity during chronic organophosphate pesticide intoxication in mice and goats     

G J Jha  L M Mahto  R K Tamang  M K Gupta  H V Chauhan 《Acta veterinaria Hungarica》1990,38(1-2):55-60

The status of cell-mediated immunity (CMI) after pesticide exposure was assessed in mice with the help of skin sensitivity and graft versus host reaction tests. It was observed that at 24 hours post-challenge CMI values did not differ significantly from the control, indicating no effect of quinalphos treatment in mice. Goats receiving monocrotophos at a dose rate of 1.0 mg kg-1 body mass for 40 days gave a similar result when CMI was tested with the help of the chemical sensitizer dinitrofluorobenzene (DNFB). Thus the results clearly indicate that the tested organophosphates do not interfere with cellular immunity in the intoxicated animals.  相似文献   

A methylflavan with free radical scavenging properties from Pancratium littorale   总被引：1，自引：0，他引：1  

Ioset JR  Marston A  Gupta MP  Hostettmann K 《Fitoterapia》2001,72(1):35-39

The isolation of 7,4'-dihydroxy-8-methylflavan (1) from the dichloromethane extract of Pancratium littorale stem was guided by an assay for free radical scavenging activity towards the 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH). The structure of 1 was established by spectrometric methods including UV, EI mass spectrometry, 1H and 13C-NMR. The free radical scavenging properties of 1 were quantified in solution using spectrophotometry.  相似文献   

Antibacterial activity of Terminalia pallida     

Gupta M  Mazumder UK  Manikandan L  Bhattacharya S  Haldar PK  Roy S 《Fitoterapia》2002,73(2):165-167

The methanol extract of the dried fruit powder of Terminalia pallida was evaluated for antimicrobial activity. The methanol extract showed a broad spectrum of antibacterial activity.  相似文献   

Experimental evaluation of Pentas lanceolata flowers for wound healing activity in rats     

Nayak BS  Vinutha B  Geetha B  Sudha B 《Fitoterapia》2005,76(7-8):671-675

The ethanolic extract of the flowers of Pentas lanceolata given by oral route to rats at dose of 150 mg kg(-1) day(-1) for 10 days, was studied for its effect on wound healing, using excision wound mode. Significant increase in granulation tissue weight, tensile strength, hydroxyproline and glycosaminoglycan content was observed. Moderate increase in protein content was seen. There was significant reduction in the wound area measurement of the test group as compared to that of controls. The efficacious prohealing action may be due to increased collagen deposition as well as better alignment and maturation.  相似文献   

Early introduction of water and complementary feeding and nutritional status of children in northern Senegal     

Gupta N  Gehri M  Stettler N 《Public health nutrition》2007,10(11):1299-1304

OBJECTIVE: Malnutrition is responsible globally for 60% of deaths among children under 5 years and is often attributed to suboptimal feeding practices. In response, the World Health Organization recommends exclusive breast-feeding for the first 6 months of life. The objective of this study was to determine if an association exists between the early introduction of water and complementary foods (CFs) and the nutritional status of children in northern Senegal. DESIGN/SETTING/SUBJECTS: A cross-sectional study of 374 children in the Podor Health District between the ages of 6 and 23 months was conducted. Knowledge and behaviours of mothers regarding introduction of water and CFs were assessed via individual interviews. RESULTS: Water was introduced to about 85% of the children in the first 3 months of life and 62% were fed CFs before 6 months. Overall, 16% had clinically significant wasting (weight-for-length Z-score (WHZ) less than - 2) and 20% had stunting (height-for-age Z-score (HAZ) less than - 2). There was no significant association between wasting or stunting and introduction of water before 3 months (WHZ: odds ratio = 0.99, 95% confidence interval 0.46-2.14, P = 0.97; HAZ: 0.68, 0.34-1.36, P = 0.3) or introduction of CFs before 6 months (WHZ: 0.81, 0.46-1.42, P = 0.5; HAZ: 0.79, 0.46-1.35, P = 0.4). A significant association was found between wasting and male sex, age, living in Guede community, drinking river/pond water and large family size, while stunting was associated with age and drinking tap water. CONCLUSION: The results of the present study suggest that early introduction of water and CFs is frequent and is not associated with increased risk for malnutrition among children from this region of northern Senegal, but the possibility of reverse causality cannot be excluded.  相似文献   

Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress   总被引：2，自引：0，他引：2  

Paromita Das  Akhil Gupta  & Sanjib Kumar Manna 《Aquaculture Research》2005,36(6):525-529

Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg^?1 total protein in kidney and gill and 4.2–5.3 ng μg^?1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills.  相似文献   

Negative interference of icteric serum on a bichromatic biuret total protein assay     

Aradhana Gupta  Steven L. Stockham 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(3):422-427

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