Stress mitigating and immunomodulatory effect of dietary pyridoxine in Labeo rohita (Hamilton) fingerlings     

Mohammad Shahbaz Akhtar  Asim Kumar Pal  Narottam Prasad Sahu  Ciji Alexander  Sanjay Kumar Gupta  Arup Kumar Choudhary  Ashish Kumar Jha  Mysore Govindrajan Rajan 《Aquaculture Research》2010,41(7):991-1002

A 60‐day experiment was carried out to delineate stress mitigating and immunomodulatory role of dietary pyridoxine (PN) in Labeo rohita fingerlings exposed to endosulfan. Two hundred and seventy fingerlings were randomly distributed into six treatments in triplicates. Five iso‐caloric and iso‐nitrogenous purified diets were prepared with graded levels of pyridoxine. Six treatment groups were T₀ (10 mg PN+without endosulfan), T₁ (0 mg PN+endosulfan), T₂ (10 mg PN+endosulfan), T₃ (50 mg PN+endosulfan), T₄ (100 mg PN+endosulfan) and T₅ (200 mg PN+endosulfan). The role of pyridoxine on stress mitigation and immunomodulation was assessed by biochemical and haemato‐immunological parameters like aspartate aminotransaminase, alanine aminotransaminase, lactate dehydrogenase, malate dehydrogenase, superoxide dismutase and catalase were significantly (P<0.05) lower while acetylcholinesterase was significantly (P<0.05) higher in pyridoxine‐fed groups. Erythrocytes count, haemoglobin content and total serum protein, albumin, globulin, nitroblue tetrazolium and lysozyme activity were significantly (P<0.05) higher while cortisol and blood glucose were decreased significantly (P<0.05) in pyridoxine‐fed groups. Percentage survival after challenge with Aeromonas hydrophila was highest in T₀ group. The results obtained in present study indicate that dietary pyridoxine supplementation at 100 mg PN kg^?1 diet reduces the endosulfan‐induced stress and triggers immune response in L. rohita fingerlings.  相似文献   

Haemato-biochemical,non-specific immunity,antioxidant capacity and histopathological changes in <Emphasis Type="Italic">Labeo rohita</Emphasis> fingerlings fed rubber protein isolate     

Femi John Fawole  N. P. Sahu  K. K. Jain  S. Gupta  K. V. Rajendran  N. Shamna  Nalini Poojary 《Fish physiology and biochemistry》2017,43(3):677-690

A 60-day feeding trial was conducted to evaluate the haemato-biochemical, innate immune response, antioxidant capacity and histopathological changes in Labeo rohita fingerlings fed rubber protein isolates (RPI). One hundred and eighty fingerlings (average weight 4.45 ± 0.01 g) were distributed into five experimental groups in triplicate and fed with isonitrogenous and isocaloric diets. Soybean protein isolate (SPI) served as the reference diet (Control), and the treatment diets were formulated as RPI₂₅, RPI₅₀, RPI₇₅ and RPI₁₀₀ replacing 25, 50, 75 and 100% of SPI protein, respectively. The growth performance indices like final body weight (9.54–10.27 g), net weight gain (5.09–5.84 g), metabolic growth rate (4.54–5.02) and feed efficiency ratio (0.60–0.65) among the various groups were not significantly different (P > 0.05). All the haematological parameters, except red blood cells, showed no significant differences compared with the control group (P > 0.05). The immuno-biochemical parameters like albumin, globulin, total immunoglobulin, respiratory burst and lysozyme activities among the various groups did not differ significantly (P > 0.05). The stress enzyme such as superoxide dismutase (SOD), catalase (CAT) and oxidative stress marker malondialdehyde (MDA) showed no significant difference (P > 0.05). Histopathological examination of the liver revealed no marked changes. In summary, the results showed that RPI was well utilised by the fish and its inclusion did not generate any oxidative-induced stress, thus, RPI may be suggested as a potential replacement for SPI in fish diets without any detrimental effects. Hence, protein isolation offers a unique opportunity for the utilisation of rubber seed meal.  相似文献   

Sequence variation in genes encoding miRNAs/targets and other related approaches for possible use in crop improvement     

Tinku Gautam  Pushpendra Kumar Gupta 《Plant Breeding》2020,139(1):28-41

  相似文献   

Delineating Genotype × Environment interactions towards durable resistance in mungbean against Cercospora leaf spot (Cercospora canescens) using GGE biplot     

Arpita Das  Sanjeev Gupta  Ashok K. Parihar  Deepak Singh  Ramesh Chand  Aditya Pratap  Kansam Dayamoy Singha  Kailash Pati Singh Kushwaha 《Plant Breeding》2020,139(3):639-650

Cercospora leaf spot (Cercospora canescens) is a major fungal disease which impedes mungbean production worldwide. Presence of wider host range with existence of pathogenic variability creates intricacy towards host-pathogen dynamics. Moreover, environmental factors having crucial role in augmenting severity of this disease further complicate disease management. An attempt has been made for unfolding genotype x environment interactions towards identifying and validating durable resistant genotypes against cercospora leaf spot in multi-environment testing. Preliminary screening with 246 genotypes under artificial epiphytotic condition was conducted to extract out a subset of 22 mungbean genotypes for further evaluation in field testing across six environments consecutively for two years. GGE biplot analysis detected significant environmental influence towards genotypic response and confirmed the presence of non-crossover interaction with incoherent genotypic response, thus advocating the urgency for multi-location testing. GGE biplot aptly identified “LGG 460” and “COGG 912” as “ideal” and “desirable” genotypes, respectively having durable resistance and genetic homeostasis and thus suggested for their utilization in future resistance breeding programme in mungbean against cercospora leaf spot.  相似文献   

Genetic diversity and population structure of Indian soybean [<Emphasis Type="Italic">Glycine max</Emphasis> (L.) Merr.] revealed by simple sequence repeat markers     

SK Gupta  JG Manjaya 《Journal of Crop Science and Biotechnology》2017,20(3):221-231

Genetic diversity in 90 Indian soybean cultivars was assessed using 45 SSR markers distributed on 20 soybean chromosomes. Forty-five SSR markers generated 232 alleles with an average of five alleles/locus. The observed frequencies of the 232 alleles ranged from 0.01 to 0.94 with an average of 0.19. The polymorphic information content (PIC) value of the SSR markers varied from 0.10 to 0.83 with an average of 0.61 and about 71% markers have a PIC value of >0.5. In this study, 54 rare alleles including 19 genotype specific alleles were also identified. The observed hetrozygosity for SSR markers ranged from 0 to 0.11 with a mean of 0.10. Cluster analysis grouped the 90 soybean cultivars into three major clusters and principal coordinates analysis (PCoA) results were similar to those of the cluster analysis. A combination of eight SSR markers successfully differentiated all 90 soybean cultivars. The population structure analysis distributed the 90 soybean genotypes into two populations with mean alpha (α) value of 0.1873. In AMOVA analysis, proportion of variation within population was high (88%), whereas only 12% occurred among populations. In cluster and structure analyses, most of the genotypes with similar pedigree were grouped together. Soybean cultivars DS228, MACS-13, LSb-1, Hardee, Improved Pelican, and Pusa-24 were the six most genetically distinct cultivars identified. The study reported a moderate genetic diversity in Indian soybean cultivars and findings would be useful to the soybean breeders in selecting genetically distinct parents for a soybean improvement program.  相似文献   

Counting the Costs: Economic Growth and Environmental Change in Thailand,edited by J. Rigg. 1995. Institute of Southeast Asian Studies,Singapore, ISBN 981-3055-08-1 (paperback), S$49.50/US$36.50, xxi+267 pp.     

A. Gupta 《Land Degradation \u0026amp; Development》1999,10(6):593-594

  相似文献