Electron microscopy of bursa of Fabricius of chicks infected with a field strain of infectious bursal disease virus   总被引：2，自引：0，他引：2  

A. S. Panisup  B. Jrplid  K. C. Verma  G. C. Mohanty 《Acta veterinaria Scandinavica》1988,29(1):125-127

Chicks were infected in the bursa with a field strain of infectious bursal disease virus. Inter- and intracellular edema, condensation and margination of nuclear chromatin, increased number of lysosomes in macrophages, and lymphocytolytic changes appeared earliest by 8 hours post infection. Inclusions containing spheroid to hexagonal virus particles were seen in the cytoplasm of the macrophages. Multiplying virus particles in crystalline arrays arranged either in single or in multiple clusters were seen in the cytoplasm of macrophages, lymphocytes and light stained reticular epithelial cells.  相似文献   

Ultrastructural changes of Rous sarcomas exhibiting spontaneous occurrence of A-type particles in chicken     

A S Panisup  G C Mohanty  K C Verma 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1988,35(10):760-764

  相似文献   

A note on the relationship between the production rates of volatile fatty acids and their concentration,digestible organic matter and total digestible nutrient intake in buffalo calves     

D.N. Verma  U.B. Singh  Usha R. Mehra  A. Varma 《Livestock Production Science》1978,5(3):253-263

Volatile fatty acid (VFA) production rates were measured by isotope dilution technique in the rumen of buffalo calves fed on wheat straw plus concentrate, green maize, cow pea and berseem. Correlations derived between the VFA production rates and their concentration and DOM were significant except in animals fed on cow pea. The VFA production rates were also significantly correlated with the TDN intake in all the four feeds tested.The regression equations obtained for the four feeds were different, which suggested that VFA production may vary with the quantity and quality of feed digested. These experiments suggest the use of different regression equations for different feeds.  相似文献   

Soil structural improvements with the addition of Lantana camara biomass in rice-wheat cropping     

P.K. Sharma  T.S. Verma  R.M. Bhagat 《Soil Use and Management》1995,11(4):199-203

Abstract. A long-term field experiment was initiated in June 1988 in a silty clay loam soil to investigate the potential of Lantana camara, an obnoxious weed, for improving structural properties and productivity of soil in rice-wheat cropping. Lantana was incorporated into the soil 10–15 days before puddling at 10, 20 and 30 t/ha (fresh weight). At the end of the sixth cropping season, Lantana additions increased the organic carbon (OC) of the 0–15 cm soil layer by 11–24%, and of water-stable aggregates (WSA, 0.50–8.0 mm diameter) by 10–21%; OC of WSA <0.50 mm diameter remained unaffected. About 17–25% of the applied OC was retained in the soil. The OC increase resulted in a decrease in bulk density of the plough layer (0–15 cm) by 7%, a decrease in aggregates of 2–8 mm diameter and of clods by 4% and 6%, respectively. There was an increase in water-stable aggregates and aggregate porosity, and a decrease in clod-breaking strength from 420 to 216 kPa. Soil cracking at the surface changed from wide, deep cracks in hexagonal pattern to a close-spaced network of fine cracks. Lantana additions increased <5mm wide cracks at the expense of 10–20 mm wide cracks; 5–10 mm wide cracks remained unchanged. Total volume of cracks decreased by 36% and surface area of cracks by 55% compared with the control plots.  相似文献   

Pharmacokinetics and urinary excretion of gentamicin in Bubalus bubalis calves following intramuscular administration     

S K Garg  S P Verma  B D Garg 《Research in veterinary science》1991,50(1):102-105

The pharmacokinetics and urinary excretion of gentamicin was studied in buffalo calves after a single intramuscular administration (10 mg kg-1). Kinetic determinants were calculated by using a two compartment open model. The absorption (t1/2Ka) and biological half lives (t1/2 beta) were calculated to be 0.43 +/- 0.08 and 3.79 +/- 0.23 h, respectively. The value of the apparent volume of distribution (VdB) was found to be 0.38 +/- 0.07 litre kg-1. The satisfactory intramuscular dosage regimen of gentamicin for buffalo calves would be 3.23 mg kg-1 as priming dose and 2.88 mg kg-1 as maintenance dose to be repeated at 12 hour intervals to achieve and maintain the therapeutic plasma levels within safe limits. Urinary excretion of gentamicin was very rapid during the first 12 hours as 48.07 +/- 1.39 per cent of the total administered dose was excreted unchanged during this period.  相似文献   

An outbreak of listeriosis in chickens     

M S Nagi  J D Verma 《The Indian veterinary journal》1967,44(7):539-543

  相似文献   

Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR     

Bakshi CS  Shah DH  Verma R  Singh RK  Malik M 《Veterinary microbiology》2005,109(3-4):211-216

The aim of this work was the design and validation of a rapid and easy single tube multiplex-PCR (m-PCR) assay for the unequivocal differential detection of Mycobacterium bovis and Mycobacterium tuberculosis. Oligonucleotide primers were based on the uninterrupted 229-bp sequence in the M. bovis genome and a unique 12.7-kb insertion sequence from the M. tuberculosis genome, which is responsible for species-specific genomic polymorphism between these two closely related pathogens. The m-PCR assay was optimized and validated using 22 M. bovis and 36 M. tuberculosis clinical strains isolated from diverse host species and 9 other non-tuberculous mycobacterial (NTM) strains. The designed primers invariably amplified a unique 168-bp (M. bovis-specific) and 337-bp (M. tuberculosis-specific) amplicon from M. bovis and M. tuberculosis strains, respectively. The accuracy of the assay, in terms of specificity, was 100%, as none of the NTM strains tested revealed any amplification product. As little as 20 pg of genomic DNA could be detected, justifying the sensitivity of the method. The m-PCR assay is an extremely useful, simple, reliable and rapid method for routine differential identification of cultures of M. bovis and M. tuberculosis. This m-PCR may be a valuable diagnostic tool in areas of endemicity, where bovine and human tuberculosis coexist, and the distinction of M. bovis from M. tuberculosis is required for monitoring the spread of M. bovis to humans.  相似文献   

Diagnosis of canine Hepatozoon spp. infection by quantitative PCR     

Li Y  Wang C  Allen KE  Little SE  Ahluwalia SK  Gao D  Macintire DK  Blagburn BL  Kaltenboeck B 《Veterinary parasitology》2008,157(1-2):50-58

Hepatozoon (H.) americanum and H. canis are the etiological agents of canine hepatozoonosis, a disease that is found worldwide and is also prevalent in the southeastern United States. Current laboratory diagnosis of canine hepatozoonosis caused by H. americanum is usually dependent on visual identification of Hepatozoon "onion skin cysts" in muscle biopsies, an approach that requires invasive sampling and can result in false negatives. We have developed a diagnostic method for detection of Hepatozoon spp. DNA that integrates nucleic acid extraction with extensive agitation to maximize DNA extraction efficiency. The DNA extracted from canine EDTA-whole blood is subjected to real-time PCR, and fluorescence resonance energy transfer (FRET) probes detect a signature polymorphism in the amplified DNA. This PCR method amplifies a fragment of the Hepatozoon 18S rDNA gene, detects as few as 7 genomic copies of Hepatozoon spp. per ml of blood with high specificity, and differentiates between H. americanum and H. canis amplicons. A surprising 300-fold increase of H. americanum 18S rDNA targets occurred during 3-0 days of storage of positive blood specimens. Examination of 614 EDTA-blood samples submitted mostly from the southeastern Unites States from dogs with suspected hepatozoonosis identified H. americanum in 167 samples (27.2%). An additional 14 samples (2.3%) were positive for H. canis, and 14 samples (2.3%) were positive for both H. americanum and H. canis. These results suggest that the Hepatozoon spp. 18S rDNA quantitative PCR may be a valuable tool that can improve diagnosis and therapy of canine hepatozoonosis.  相似文献   

Field performance of somaclones of rose scented geranium (Pelargonium graveolens L’Her Ex Ait.) for evaluation of their essential oil yield and composition     

Gauri Saxena  Praveen Chandra Verma  Suchitra Banerjee  Sushil Kumar 《Industrial Crops and Products》2008,27(1):86-90

Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium graveolens L’Her Ex Ait. cv. Hemanti were successfully transferred to the field in Northern India for evaluation. Two distinct morphotypes were described on the basis of leaf dentation-one resembling the parental cultivar in having highly dentated leaves (HDL) and the other with less dentated leaves (LDL). After repeated field-testing for 3 consecutive years, the HDL clones closely resembled the parental cultivar with respect to the different quantity and quality determining traits, while the LDL group was clearly different. The field established LDL somaclones possessed higher herb yield, number of branches and other oil yield attributing traits as compared to the HDL clones and the parent cultivar. The chemical investigations of the essential oil revealed significant differences between the LDL clones, the HDL clones and the control. Selection of such somaclones, which are superior to the parental in most of the quantitative and qualitative traits and show better adaptability to different areas of cultivation, will help towards commercialization of geranium in India.  相似文献   

Enhanced disease resistance in the Indian snakehead,Channa punctata against Aeromonas hydrophila,through 5 % feed supplementation with F. benghalensis (aerial root) and L. leucocephala (pod seed)     

Vipin Kumar Verma  Kumari Vandana Rani  Neeta Sehgal  Om Prakash 《Aquaculture International》2015,23(5):1127-1140

  相似文献