Development and characterization of microsatellite markers,genetic diversity and population structure analysis in Sapota (Manilkara zapota (L.) P. Royen)     

Sathanandam  Pavithra N.  Patil  Prakash  Rekha  Ajitha  Muthuvel  Iyamperumal  Patel  Amrutlal R.  Boggala  Ramesh B.  Shirol  Adiveppa M.  Ravishankar  Kundapura V. 《Genetic Resources and Crop Evolution》2022,69(8):2787-2801

Genetic Resources and Crop Evolution - Manilkara zapota (L.) P. Royen, a widely adapted and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite...  相似文献   

BOOK REVIEW: Soil Analysis. Edited by S. K. Singh,D. R. Biswas,C. A. Srinivasamurthy,S. P. Datta,G. Jayasree,P. Jha,S. K. Sharma,R. N. Katkar,K. P. Raverkar,A. K. Ghosh,Indian Society of Soil Science,New Delhi,India, 2019, pp. INR1400, ISBN 81-903797-8-X.     

Sabyasachi Majumdar  Laxmanarayanan Muruganantham  Kapudeep Karmakar  Prakash Nagabovanalli Basavarajappa 《European Journal of Soil Science》2022,73(4):e13259

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Phylogenetic studies in Citrus reticulata and related types     

H. C. Dass  G. S. Randhawa  D. Prakash 《Euphytica》1977,26(3):805-810

Summary Sixteen types of mandarins were screened for their leaf flavonoid patterns with the help of cellulose thin-layer chromatography. In general, Tanaka's classification of mandarins into 36 species is untenable, as was already concluded by Swingle (1948), Hodgson (1965) and Singh (1967). It is likely that a fine loose skinned mandarin was first evolved. Later, it hybridized with wild mandarin to give rise to numerous types and forms.Contribution No. 474 of Indian Institute of Horticultural Research, Bangalore-6, India.  相似文献   

A unique introgression from Moricandia arvensis confers male fertility upon two different cytoplasmic male-sterile lines of Brassica juncea     

S. R. Bhat    S. Prakash    P. B. Kirti    V. Dinesh  Kumar V. L. Chopra 《Plant Breeding》2005,124(2):117-120

A Brassica juncea line carrying an introgression from Moricandia arvensis restored male fertility to two cytoplasmic male‐sterile (CMS) B. juncea lines carrying either M. arvensis or Diplotaxis catholica cytoplasm. Genetics of fertility restoration was studied in the F₁, F₂, F₃ and backcross generations of the cross between CMS and fertility‐restorer lines. No male‐sterile plants were found in F1‐F₃ generations of the cross between CMS [M. arvensis] B. juncea and the restorer. However, a 1: 1 segregation for male sterility and fertility was observed when the F1 was pollinated with non‐restorer pollen from a euplasmic line. These results clearly show that restoration is mono‐genic and gametophytic. In CMS lines carrying D. catholica cytoplasm, the restorer conferred male fertility to the F1 and showed 3: 1 and 1: 1 segregations for male fertility and sterility in F₂ and BC1 generations, respectively, indicating a monogenic, sporophytic mode of fertility restoration. The results were also supported by pollen stainability in the F1 which was about 65% in M. arvensis‐based CMS and >90% in D. catholica‐based CMS. The above results are discussed in the light of previous molecular studies which showed association between CMS and atpA in both systems.  相似文献   

Corrigendum to “Catastrophic collapse of Indian white-backed Gyps bengalensis and long-billed Gyps indicus vulture populations” [Biol. Conserv. 19 (2003) 381-390]     

V. Prakash  A.A. Cunningham  N. Prakash  R. Gargi  A.R. Rahmani 《Biological conservation》2005,124(4):559

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Tyrosine cross-links: molecular basis of gluten structure and function     

Tilley KA  Benjamin RE  Bagorogoza KE  Okot-Kotber BM  Prakash O  Kwen H 《Journal of agricultural and food chemistry》2001,49(5):2627-2632

The formation of the large protein structure known as "gluten" during dough-mixing and bread-making processes is extremely complex. It has been established that a specific subset of the proteins comprising gluten, the glutenin subunits, directly affects dough formation and breadmaking quality. Glutenin subunits have no definitive structural differences that can be directly correlated to their ability to form gluten and affect dough formation or breadmaking quality. Many protein structural studies, as well as mixing and baking studies, have postulated that disulfide bonds are present in the gluten structure and contribute to the process of dough formation through the process of disulfide-sulfhydryl exchange. Evidence presented here indicates that tyrosine bonds form in wheat doughs during the processes of mixing and baking, contributing to the structure of the gluten network. The relative contributions of tyrosine bonds and disulfide--sulfhydryl interchange are discussed.  相似文献   

Assessment of genetic relationships between <Emphasis Type="Italic">Rhus</Emphasis> L. species using RAPD markers     

Surya Prakash  Johannes Van Staden 《Genetic Resources and Crop Evolution》2007,54(1):7-11

Genetic diversity of seven Rhus L. species was assessed using random amplified polymorphic DNA (RAPDs) markers. Initially, 90 primers were screened, of which 25 produced reproducible amplification products. These primers generated a total of 296 bands, with an average of 11.8 bands per primer. Out of 296 bands scored, 236 (80%) were polymorphic and 62 (20%) were monomorphic. Primers OPC-05 and OPD-05 generated 100% polymorphic bands. The resolving power of primers ranged from 9.4 to 26.8. Similarity matrix values ranged from 0.45 to 0.63. The dendrogram generated using Unweighted Pair Group Method using Arithmetic Averages (UPGMA) grouped all the species of Rhus in one major group with two sister groups, whilst R. pyroides Burch. and R. dentata Thunb. were outliers. R. gerrardii (Harv. ex Engl.) Diels, R. glauca Thunb. and R. pentheri Zahlbr. constituted one sister group, while R. natalensis Bernh. ex C. Krauss and R. gueinzii Sond. were included in the other. The degree of genetic diversity observed between seven species of Rhus with RAPD markers suggest that this approach could be used for studying the phylogeny of the genus.  相似文献   

Involvement of mammalian Mus81 in genome integrity and tumor suppression     

McPherson JP  Lemmers B  Chahwan R  Pamidi A  Migon E  Matysiak-Zablocki E  Moynahan ME  Essers J  Hanada K  Poonepalli A  Sanchez-Sweatman O  Khokha R  Kanaar R  Jasin M  Hande MP  Hakem R 《Science (New York, N.Y.)》2004,304(5678):1822-1826

Mus81-Eme1 endonuclease has been implicated in the rescue of stalled replication forks and the resolution of meiotic recombination intermediates in yeast. We used gene targeting to study the physiological requirements of Mus81 in mammals. Mus81-/- mice are viable and fertile, which indicates that mammalian Mus81 is not essential for recombination processes associated with meiosis. Mus81-deficient mice and cells were hypersensitive to the DNA cross-linking agent mitomycin C but not to gamma-irradiation. Remarkably, both homozygous Mus81-/- and heterozygous Mus81+/- mice exhibited a similar susceptibility to spontaneous chromosomal damage and a profound and equivalent predisposition to lymphomas and other cancers. These studies demonstrate a critical role for the proper biallelic expression of the mammalian Mus81 in the maintenance of genomic integrity and tumor suppression.  相似文献   

Tumor suppression with a combination of alpha-difluoromethyl ornithine and interferon   总被引：1，自引：0，他引：1  

P S Sunkara  N J Prakash  G D Mayer  A Sjoerdsma 《Science (New York, N.Y.)》1983,219(4586):851-853

alpha-Difluoromethyl ornithine and mouse type 1 interferon, when administered simultaneously, were highly toxic to B16 melanoma cells in culture. Oral administration of alpha-difluoromethyl ornithine suppressed B16 melanoma development in mice 85 percent whereas interferon given subcutaneously inhibited tumor growth only 24 percent. Total or near total suppression of tumor growth was observed in mice receiving both treatments.  相似文献   

Microfluidic bubble logic     

Prakash M  Gershenfeld N 《Science (New York, N.Y.)》2007,315(5813):832-835

We demonstrate universal computation in an all-fluidic two-phase microfluidic system. Nonlinearity is introduced into an otherwise linear, reversible, low-Reynolds number flow via bubble-to-bubble hydrodynamic interactions. A bubble traveling in a channel represents a bit, providing us with the capability to simultaneously transport materials and perform logical control operations. We demonstrate bubble logic AND/OR/NOT gates, a toggle flip-flop, a ripple counter, timing restoration, a ring oscillator, and an electro-bubble modulator. These show the nonlinearity, gain, bistability, synchronization, cascadability, feedback, and programmability required for scalable universal computation. With increasing complexity in large-scale microfluidic processors, bubble logic provides an on-chip process control mechanism integrating chemistry and computation.  相似文献