Hand-in-hand in the "trading zone"     

Higgins J 《Science (New York, N.Y.)》2003,299(5613):1625

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Sampling Strategies for Mapping Soil Phosphorus and Soil Potassium Distributions in Cool Temperate Grassland   总被引：1，自引：0，他引：1  

Shi  Z.  Wang  K.  Bailey  J. S.  Jordan  C.  Higgins  A. J. 《Precision Agriculture》2000,2(4):347-357

Unlike the situation for arable soils, virtually nothing is known about the spatial dependencies of soil properties in cool temperate grassland or about what the optimal sampling strategies ought to be for mapping soil nutrient distributions in such situations. The aim of this study was to investigate the spatial variability in plant-available (soil) phosphorus and potassium in a grass silage field in Northern Ireland and devise optimal sampling strategies for mapping their distributions. Soil samples were collected from the field at 25 m intervals in a regular rectangular grid to provide a database of soil properties. Different data combinations were subsequently abstracted from this database for comparison purposes, and ordinary kriging used to produce interpolated soil maps. Soil potassium displayed greater spatial variability than soil phosphorus. In keeping with this observation, the results of three separate statistical procedures demonstrated that the optimal sample size for estimating the true population means was about twice as large for soil potassium as for soil phosphorus. Optimal sampling strategies, however, related not just to sample size but to sample combination and field shape as well.  相似文献   

The structure of D. radiodurans     

Battista JR  Cox MM  Daly MJ  Narumi I  Radman M  Sommer S 《Science (New York, N.Y.)》2003,302(5645):567-8; author reply 567-8

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Fungal Morphogenesis: Ring Formation and Closure by Arthrobotrys dactyloides     

Higgins ML  Pramer D 《Science (New York, N.Y.)》1967,155(3760):345-346

The formation and the closure of constricting rings by a nematode-trapping fungus were recorded by means of time-lapse cinephotomicrography. Analysis of the film revealed that hyphal rings resulted from a sequence of morphological events not previouisly described. Cell inflation and ring constriction were induced by touch, increased temperature, and electrical stimutlation. The inflation process was not particularly sensitive to metabolic inhibitors and appears to operate without an expenditutre of energy on the part of the cell.  相似文献   

THE GERMINATION OF SEEDS,GROWTH OF PLANTS AND DEVELOPMENT OF CHLOROPHYLL AS INFLUENCED BY SELECTIVE SOLAR IRRADIATION     

Sheard C  Higgins GM  Foster WI 《Science (New York, N.Y.)》1930,71(1837):291-293

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Microbiology. A hitchhiker's guide to type IV secretion     

Lybarger SR  Sandkvist M 《Science (New York, N.Y.)》2004,304(5674):1122-1123

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Use of pimobendan in 170 cats (2006–2010)     

John M. MacGregor  John E. Rush  Nancy J. Laste  Rebecca L. Malakoff  Suzanne M. Cunningham  Natalie Aronow  Daniel J. Hall  Justin Williams  Lori L. Price 《Journal of Veterinary Cardiology》2011,13(4):251-260

Hypothesis/ObjectivesTo describe the therapeutic use of pimobendan in cats, describe the patient population to which it was administered, document potential side effects and report the clinical course following administration of pimobendan in conjunction with standard heart failure therapy. It is hypothesized that cats with advanced heart disease including congestive heart failure from a variety of causes will tolerate pimobendan with a minimum of side effects when used in treatment in conjunction with a variety of other medications.Animals, materials and methodsOne hundred and seventy client owned cats with naturally occurring heart disease, one hundred and sixty four of which had congestive heart failure. Medical records were reviewed and owners and referring veterinarians were contacted for follow-up data. Data collected included pimobendan dose, other medications administered concurrently, data collected at physical examination, presence or absence of heart failure, adverse effects, classification of heart disease, echocardiographic data and survival time. The data were analyzed for significance between the initial visit and any follow-up visits.ResultsAll cats were treated with pimobendan. The median pimobendan dose was 0.24 mg/kg q 12 h. Pimobendan was used in combination with multiple concurrent medications including angiotensin converting enzyme inhibitors, diuretics and anti-thrombotics. Five cats (3.0%) had potential side effects associated with pimobendan. One cat (0.6%) had presumed side effects severe enough to discontinue pimobendan use. Median survival time for 164 cats with congestive heart failure after initiation of pimobendan was 151 days (range 1–870).ConclusionPimobendan appears to be well tolerated in cats with advanced heart disease when used with a variety of concurrent medications. Randomized controlled studies need to be performed to accurately assess whether it is efficacious for treatment of congestive heart failure in cats.  相似文献   

Variation in the flavonol glycoside composition of almond seedcoats as determined by maldi-tof mass spectrometry     

Frison S  Sporns P 《Journal of agricultural and food chemistry》2002,50(23):6818-6822

Seedcoats of 16 almond varieties were screened for flavonol glycosides by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Flavonol glycosides were extracted by a simple methanolic extraction followed by a quick cleanup procedure with a Sep-Pak C(18) cartridge. Each of the 16 seedcoat samples exhibited a unique composition. Four flavonol glycosides, isorhamnetin rutinoside, isorhamnetin glucoside, kaempferol rutinoside, and kaempferol glucoside, were detected and quantified with use of rutin as an internal standard. Individual peak ratios were very consistent across triplicate analyses of all samples; the average standard deviation was 9%. In all almond varieties, isorhamnetin rutinoside was the most abundant flavonol glycoside, and the total content ranged from 75 to 250 microg/g.  相似文献   

Biochemical characterization of amandin,the major storage protein in almond (Prunus dulcis L.)   总被引：1，自引：0，他引：1  

Sathe SK  Wolf WJ  Roux KH  Teuber SS  Venkatachalam M  Sze-Tao KW 《Journal of agricultural and food chemistry》2002,50(15):4333-4341

The almond major storage protein, amandin, was prepared by column chromatography (amandin-1), cryoprecipitation (amandin-2), and isoelectric precipitation (amandin-3) methods. Amandin is a legumin type protein characterized by a sedimentation value of 14S. Amandin is composed of two major types of polypeptides with estimated molecular weights of 42-46 and 20-22 kDa linked via disulfide bonds. Several additional minor polypeptides were also present in amandin. Amandin is a storage protein with an estimated molecular weight of 427,300 +/- 47,600 Da (n = 7) and a Stokes radius of 65.88 +/- 3.21 A (n = 7). Amandin is not a glycoprotein. Amandin-1, amandin-2, and amandin-3 are antigenically related and have similar biochemical properties. Amandin-3 is more negatively charged than either amandin-1 or amandin-2. Methionine is the first essential limiting amino acid in amandin followed by lysine and threonine.  相似文献   

Monitoring acetylcholinesterase levels in migrant agricultural workers and their children using a portable test kit     

Higgins GM  Muñiz JF  McCauley LA 《Journal of agricultural safety and health》2001,7(1):35-49

The EQM Research, Inc., portable test kit was evaluated as a surveillance tool for blood cholinesterase levels among migrant workers and their children. Laboratory validation demonstrated a linear relationship between the reference Ellman and kit methods (Ellman = 0.95 x kit result + 0.82, r2 = 0.98). Pre- and post-season cholinesterase levels measured in 70 farm workers were within normal ranges, but significantly different at 28.5 and 29.7 U/g Hb, respectively (paired t-test, p = 0.014). Results from 98 migrant farm worker children and a comparison group of 53 age-matched non-agricultural children showed that cholinesterase levels were not significantly different between the agricultural and non-agricultural children (ANOVA, p = 0.69). These data demonstrate that a portable test kit can provide useful data pesticide exposures when measurements are made in a temperature-controlled setting.  相似文献