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91.
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93.
F. Kristensen B. Kristensen M. Vandevelde R.J. Higgins A.L. De Weck 《Veterinary immunology and immunopathology》1981,2(6):579-590
Lymphocytes from dog thymus and mesenteric lymphnodes have been stimulated in vitro with 3 different mitogens. Culture medium was enriched with either autologous plasma, fetal calf serum or a newly described defined serum substitute. In such cultures the number of surviving and activated cells was quantified by cytofluorometry and the proliferation was assessed by thymidine incorporation. Results obtained with the 3 media were very similar. A significant number of lymphocytes died during the first 42 hours of incubation. There was a tendency toward more surviving cells with fetal calf serum and the serum substitute, whereas more activated (G1) cells and higher thymidine incorporation could be observed with autologous plasma. Furthermore, when results obtained with various mitogen concentrations and from individual dogs were analyzed, a high correlation between “highly activated (G1b) cells and thymidine incorporation was found, i.e. r=0.84 for thymocytes and 0.68 for lymphnode cells. The correlation between all G1 (G1a+b) cells and thymidine incorporation was lower or absent (r=0.02 and 0.55, respectively). It is concluded from these results that the population of G1b cells have received all required signals necessary for proliferation whereas the total G1 cell population also include activated cells, which are not obligatorily undergoing subsequent proliferation 相似文献
94.
Enzyme-linked immunosorbent assay for the detection of antibodies to bovine virus diarrhea virus in sera from border disease virus-infected sheep.
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H J Chu M M Sawyer C A Anderson R J Higgins Y C Zee 《Canadian journal of veterinary research》1987,51(2):281-283
An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific antibodies against the causative agent of border disease in ovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified bovine virus diarrhea virus was used as test antigen. The optimal amount of antigen was 0.5 microgram/well, and the optimal concentration of conjugate was at 1/4,000 dilution. A total of 20 ovine serum samples, which had been collected from animals with or without border disease, were compared by ELISA and serum neutralization test for the detection of border disease-specific antibodies. ELISA was shown to be equally specific but less time-consuming and easier to perform than serum neutralization test. A positive correlation (r = 0.60) between the two tests was found. 相似文献
95.
Changes in the susceptibility of Actinobacillus pleuropneumoniae to antimicrobial agents in Quebec (1981-1986) 总被引:1,自引:0,他引:1
J P Vaillancourt R Higgins G P Martineau K R Mittal S Larivière 《Journal of the American Veterinary Medical Association》1988,193(4):470-473
An important reduction in the in vitro efficacy of spectinomycin and chloramphenicol was recorded between 1981 and 1986 against the causal agent of porcine pleuropneumonia Actinobacillus. Antimicrobial susceptibility tests were carried out by use of Kirby-Bauer disk diffusion technique on a total of 723 isolates of Actinobacillus pleuropneumoniae. Results did not agree with those of other reports in which a constant susceptibility to any of the antimicrobial agents tested was reported with serotype 2 isolates. The ability to acquire drug resistance may differ from one serotype to another. 相似文献
96.
B. Kristensen F. Kristensen M. Vandevelde R.J. Higgins A.L. de Weck 《Veterinary immunology and immunopathology》1982,3(4):439-448
Lymphocytes from dog peripheral blood have been stimulated in vitro with 3 different mitogens (Con A, PHA and PWM). Culture medium was RPMI 1640 enriched with either autologous plasma, fetal calf serum or a newly described defined serum substitute. In such cultures the number of surviving and activated cells was measured by cytofluorometry and the proliferation was assessed by thymidine incorporation. In unstimulated cultures, up to 70% of all cells had disappeared (died) during the first 42 hours of incubation, whereas the number of viable cells was reduced to 50–60% in mitogen stimulated cultures. Of the surviving lymphocytes, between 25–40% of the cells appeared to have an elevated RNA-content (activated or G1 cells). By comparison between thymidine incorporation and number of mitogen induced G1 cells, a very high correlation was found (r=0.92). However, the slope of the regression line was much lower than expected. The low thymidine incorporation per activated cell was primarily related to the high cell death and a resulting dilution of tritiated thymidine. Indeed, preliminary results suggested that the same thymidine incorporation per G1b cells could be obtained if peripheral blood lymphocytes were washed immediately before pulsing as could be obtained with lymphnode cells without washing. 相似文献
97.
R. Higgins 《The Canadian veterinary journal. La revue veterinaire canadienne》1981,22(9):277-278
Hemorrhagic diathesis is one of the most striking manifestations in acute leptospirosis. Hemorrhages are seen in infections due to Leptospira icterohaemorrhagiae as well as in those caused by Leptospira pomona. Thrombocytopenia is a constant feature and its finding can be useful for the diagnosis. Attempts to demonstrate the presence of a toxin in leptospires were unsuccessful. A few years ago, a syndrome of disseminated intravascular coagulation was associated with the physiopathogenesis of experimental leptospirosis with L. icterohaemorrhagiae. More recently, this syndrome was identified in cases of human leptospirosis and in hamsters infected with L. pomona. It appears now that other spirochetal infections (borreliosis) have a similar pathogenesis. Nonetheless, many points are still unclear: the primary cause of disseminated intravascular coagulation is unknown, as well as the virulence factors of spirochetes. Some points favor the presence of a toxic factor in leptospires: vascular damage that occurs in the absence of leptospires in damaged areas and the fact that antibiotic therapy is ineffective unless treatment is initiated early in the disease. 相似文献
98.
Central nervous system (CNS) tissues from 192 cats with neurological signs were examined histologically, and tissues from 173 of them were later examined immunohistochemically as part of a survey to determine the prevalence of feline spongiform encephalopathy (FSE). One of the cats was from Norway and the others were from Great Britain. The most commonly recorded clinical signs were ataxia, behavioural changes and epilepsy, but none of the cats had histopathological evidence of FSE. The most common organic CNS lesions were non-suppurative encephalomyelitis in 28 per cent, neoplasia in 15 per cent and a heterogeneous group of degenerative encephalopathies in 9 per cent of the cats. A range of minor histological lesions of uncertain significance was also observed. No histological lesions were observed in the tissues of 63 (33 per cent) of the cats. Disease-specific prion protein (PrP(Sc)) was observed in only one of the 173 cats examined by immunohistochemistry. 相似文献
99.
100.
D A Higgins 《The Veterinary record》1966,79(3):67-71