硒对母猪氧化应激及其后代抗氧化能力的营养调控研究     

赵金波  高圣玥  宋岩  王佳辉  唐玲玲  张智慧  李伟  李梦姝 《饲料研究》2021,(2):138-141

硒作为的重要微量元素之一,可以通过胎盘和乳组织进行转移。仔猪可以从乳汁中获取硒源,提高硒含量,进而提高仔猪的初生重。同时,硒也能够调节母猪妊娠期间代谢速度,控制妊娠期自由基产生,进而提高胎盘输送营养功能。文章从母猪氧化应激体系角度来解析硒对母猪妊娠期氧化应激的调控机制、硒对哺乳母猪生产性能的调控作用。  相似文献   

Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines     

Xiao-feng YUE  Yan-lun JU  Zi-zhu TANG  Ya-meng ZHAO  Xu-liang JIAO  Zhen-wen ZHANG 《农业科学学报》2019,18(9):2052-2062

The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines.  相似文献   

持续低温胁迫下大花蕙兰不同组织器官耐寒响应     

唐敏 《北方园艺》2015,(3):84-87

采用自然降温处理,分别测定了大花蕙兰地上部分和地下部分中丙二醛(MDA)含量、膜透性(CMP)、可溶性蛋白质(SP)含量、可溶性糖(SS)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和过氧化氢酶(CAT)活性等与耐寒性有关的生理指标,比较地上部分和地下部分耐寒差异性,研究低温胁迫对大花蕙兰组培苗生理特性的影响。结果表明:POD活性、可溶性蛋白质含量和可溶性糖含量在反映大花蕙兰组培苗耐寒性方面作用明显,可作为其耐寒性鉴定指标;地上部分和地下部分在耐寒性提高时表现出不一致性,后者比前者表现出更强的耐寒性能。  相似文献   

白花蛇舌草超微粉对小鼠免疫功能的影响     

王航  汤承  岳华 《中国畜牧兽医》2014,41(3):166-169

The assay was aimed to study the effect of Hedyotis diffusa Willd superfines powder on immune function. Three groups of mice were gavaged with the drugs at doses of 1.75,1.25 and 0.75 g/kg once a day for 7 consecutive days, repectively. The mice of control group were administered with the same volume of saline.At the last time, mice were injected into the muscle with Newcastle disease vaccine,and get peripheral blood on days 7, 14 and 21 after the medicine administration. The enhancement of innate immune responses were evaluated by using CCK-8 method, hemagglutination inhibition test, macrophage phagocytic function test and organ coefficient method. The levels of lymphocytes proliferative capacity, serum antibody, peritoneal macrophage phagocytosis and the indexes of immune organs in mice were extremely significantly enhanced (P＜0.01). Hedyotis diffusa dWilld superfines powder could extremely significantly regulate the immunity function of mice (P＜0.01).  相似文献   

茶树咖啡碱合成酶CRISPR/Cas9基因组编辑载体的构建   总被引：3，自引：0，他引：3  

唐雨薇  刘丽萍  王若娴  陈宇宏  刘仲华  刘硕谦 《茶叶科学》2016,(4):414-426

CRISPR/Cas9技术是一门新兴的基因组定点编辑技术,具有操作简单、高效的优点,可轻松实现对目标基因的敲除、替换和定点突变等操作。该技术刚诞生,就受到了全球生命科学领域研究者的关注,不到3年的时间就已经成功应用于多种动、植物当中。然而CRISPR/Cas9技术在茶树中的应用面临载体构建问题,本文以茶树咖啡碱合成酶为例,联合采用常规PCR、Overlapping PCR和Golden Gate Cloning技术,构建了包含茶树咖啡碱合成酶双靶点的CRISPR/Cas9基因编辑载体,为CRISPR/Cas9介导的基因组编辑技术在茶树中的应用奠定了坚实基础。  相似文献   

不同施肥方式对红壤蔬菜田氨氧化细菌和氨氧化古菌群落的影响   总被引：1，自引：0，他引：1  

周志成  罗葵  唐前君  荣湘民  刘强  何飞飞 《中国蔬菜》2015,1(7):33

通过构建氨单加氧酶基因(amoA)克隆文库,研究在红壤蔬菜田上只施用磷钾化肥(PK)、只施氮磷钾化肥(NPK)、施用腐熟有机肥(DNPK)和施用新鲜有机肥(FNPK)等4种不同施肥处理的土壤氨氧化细菌(AOB)和氨氧化古菌(AOA)群落多样性及与土壤脲酶活性的相关性。结果表明:施加有机肥处理(DNPK和FNPK)的蔬菜田土壤的AOB文库和AOA文库OTU数量和Shannon指数高于只施用无机肥(NPK和PK)处理的蔬菜田土壤;DNPK和FNPK处理的土壤优势AOB菌群为多形亚硝化叶菌(Nitrosolobus multiformis),比例分别为88.5%和68.5%,NPK和PK处理的土壤优势AOB菌群为亚硝化单胞菌属(Nitrosospirasp.),比例分别为54.8%和65.5%;DNPK、FNPK、NPK和PK处理土壤优势AOA菌群均为阿伯丁土壤亚硝化细杆菌侯选种(CandidatusNitrosotalea devanaterra),比例分别为90.9%、84.4%、77.8%和45.2%;施加有机肥处理(DNPK和FNPK)的土壤脲酶活性和氨氧化微生物的多样性指数都高于只施用无机肥处理(NPK和PK);AOA群落多样性指数与土壤脲酶活性呈显著正相关,而AOB群落多样性与土壤脲酶活性相关性不显著。总体来看,有机肥比无机肥处理提高了AOA和AOB群落多样性,且AOA在红壤蔬菜田土壤氨氧化过程中起着更为重要的作用。  相似文献   

黄土沟壑区开采沉陷对地形因子的影响研究     

汤伏全  黄韩  孙学阳  乔德京  白峰 《干旱区资源与环境》2016,(5):124-128

以黄土沟壑区的采煤沉陷区为研究对象,以地面数字高程模型和开采沉陷预计结果为基础数据,基于ArcGIS平台对煤矿开采沉陷区的地形数据进行分析处理,研究开采沉陷前、后地形因子的变化特征,并对不同地形因子之间的相关性进行了分析.研究结果表明:黄土沟壑区地面高程、坡度、坡向、起伏度等各种地形因子均受开采沉陷影响而发生改变,开采沉陷和地形因子之间存在明确的量化关系,而平均高程、起伏度、曲率、沟壑密度等因子与地面坡度之间存在显著的相关性.研究结果有助于揭示煤矿开采沉陷区的地形地貌演变规律,为保护西部矿区的生态环境提供技术支持.  相似文献   

用于构建双价禽流感DNA疫苗的双目的基因表达质粒的构建及鉴定     

黄冬  陈普成  刘兵  唐猛  柳金雄  姜永萍  陈化兰 《中国预防兽医学报》2016,(4):257-261

为探索构建禽流感双价或多价DNA疫苗的可行性,本研究以本实验室前期研制的禽流感DNA疫苗p CAGGopti HA5为基础[其中含有禽流感病毒(AIV)A/Goose/Guang Dong/1/96(H5N1)的HA基因],将增强型绿色荧光蛋白(eGFP)报告基因作为第二个目的基因,利用双启动子启动、内部核糖体进入位点(IRES)介导以及通过蛋白Linker编码序列连接融合表达两个目的基因的形式,构建了双启动子重组表达质粒pH5-eGFP、IRES介导的重组表达质粒pHIE以及融合表达重组质粒pH6E。并采用脂质体介导将这3种重组质粒分别转染293T细胞,通过间接免疫荧光试验和western blot方法检测HA和eGFP这两种目的蛋白的瞬时表达情况。结果显示,这3种重组质粒均能够正确表达AIV的HA蛋白和eGFP。其中pH5-eGFP表达出两个独立的目的蛋白,而且互相不影响各自的表达和细胞定位;pH6E则以融合形式表达两种目的蛋白,由于HA蛋白具有膜定位信号,所以融合蛋白主要定位于细胞膜上。本研究结果表明,双启动子表达质粒和双目的蛋白融合表达质粒适合用于构建AIV以及其他相关病原的双价DNA疫苗。  相似文献   

Advances on Detection Methods for Residues of Nitrofurans in Food     

WANG Ming-ming  XU Na  TANG Yun  HANG Ye  LIU Zhen-jiang  DU Dao-lin 《中国畜牧兽医》2016,43(8):2202-2207

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.  相似文献   

2012年春季塔克拉玛干沙漠腹地沙尘颗粒的组成研究     

张延伟  唐湘玲  陆辉  葛全胜  王雯雯  魏文寿 《干旱区资源与环境》2017,31(11):94-99

为研究塔克拉玛干沙漠腹地沙尘颗粒的微观形貌及其矿物、元素组成,应用扫描电镜能谱技术(SEM/EDX)对塔克拉玛干沙漠腹地塔中观测站2012年5月采集的PM10样品进行了识别和分析。统计622个单颗粒高分辨电镜图像及能谱的结果表明:塔克拉玛干沙漠大气PM10主要来源于地壳矿物。其中,矿物组成主要是石英、粘土矿物、方解石、岩盐,特别是岩盐含量高达14.5%,石英、方解石、石膏、岩盐单体矿物相对含量较高,但大部分都以矿物集合体的形式存在,与其它人为源颗粒物的混合主要是外混合。不同天气类型下,其矿物组成差异明显,重矿物(岩盐等)主要来源于强风就地扬起或携带的较粗颗粒。  相似文献