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31.
Validation of otolith daily increments for larval and juvenile Japanese halfbeak Hyporhamphus sajori
Mitsuhiro Nakaya Taizo Morioka Kyohei Fukunaga Sachio Sekiya Tadao Jinbo Hiroshi Nagashima Yasuhiro Ueno 《Fisheries Research》2008,93(1-2):186-189
Daily ring formation was verified by examining the growth of the marginal increment on sagittal otoliths of larval and juvenile Japanese halfbeak Hyporhamphus sajori. The relationship between age (x) and number of increments (y) is y = 1.0x + 2.0. The first daily increment was formed during the 2 days before hatching. The relationship between the number of rings deposited after the alizarin complexone (ALC) mark (x) and the number of increments (y) is given by: y = 1.0x − 0.2. The index of completion of the marginal increment was 99 ± 4.1% (mean ± S.D.) at 04:00, and from 24 ± 4.4% at 08:00 it increased with time of day and reached 98 ± 3.6% until the next day at 04:00. Growth of the incremental zone started a few hours after sunrise prior to which the discontinuous zone seemed to be formed. Light rhythms tend to be one of the most important factors for the formation of the marginal increment on otoliths. Based on the relationships between time of day and the marginal increment on otoliths, it would be possible to estimate the predation time for specimens retrieved from stomach contents, and also clearer analysis of the growth history immediately prior to the sampling time. 相似文献
32.
Plasma Membrane Lipids Are the Powerful Components for Early Stage Aluminum Tolerance in Triticale 总被引:1,自引:0,他引:1
Tadao Wagatsuma Satoru Ishikawa Matsuo Uemura Wataru Mitsuhashi Takeshi Kawamura Md. Shahadat Hossain Khan Keitarou Tawaraya 《Soil Science and Plant Nutrition》2005,51(5):701-704
The order of aluminum (Al) tolerance in triticale lines (ST2>ST22) after re-elongation in an Al-free 0.2 mM calcium (Ca) solution for 9 h (Ca period) following 1 h pretreatment with 20 μM Al (Al period) agreed with that after 24 h of Al treatment. Permeability of the plasma membrane (PM) of root-tip cells after the Ca period was significantly increased in Al-sensitive ST22. Al was accumulated more heavily in the root-tip portion of ST22 than in that of ST2, although similar amounts of malic and citric acid anions were released from both triticale lines. We established a new system examining lipid permeability using synthesized nylon-2,8 ultrathin and porous capsules trapped previously with 0.1% (w/v) methylene blue solution and coated thereafter with PM lipid isolated from root tips by a newly developed technique. Permeability of the PM lipid measured with time in 0.2 mM Ca with or without 50 μM Al photometrically ( A 680 ) was significantly greater in Al-sensitive ST22 after 5 min of Al treatment. This is the first report to directly show the primary and early role of PM lipid in Al tolerance in triticale. 相似文献
33.
Molecular design of antifungal agents 总被引:3,自引:0,他引:3
Kubo I Xiao P Nihei K Fujita K Yamagiwa Y Kamikawa T 《Journal of agricultural and food chemistry》2002,50(14):3992-3998
In a rational approach to the design of antifungal agents against Saccharomyces cerevisiae, a series of alkyl gallates (3,4,5-trihydroxybenzoates) were synthesized and assayed. Nonyl gallate (1) was found to be the most effective with a minimum fungicidal concentration (MFC) of 12.5 microg/mL (42 microM), followed by octyl gallate (2) with an MFC of 25 microg/mL (89 microM). These MFCs are little influenced by pH values. A time-kill curve study indicates that nonyl gallate exhibits fungicidal activity against S. cerevisiae at any growing stage. The antifungal activity of nonyl gallate is due primarily to its ability to act as a nonionic surface-active agent (surfactant). The length of the alkyl group is not a major contributor but plays a role in eliciting the activity to a large extent. As far as alkyl gallates are concerned, their antimicrobial spectra and potency depend largely on the hydrophobic portion of the molecules. 相似文献
34.
Soichi?TanakaEmail author Yuko?Fujiwara Yoshihisa?Fujii Shogo?Okumura Hiroyoshi?Togo Naoya?Kukutsu Tadao?Nagatsuma 《Journal of Wood Science》2011,57(3):189-194
The attenuation coefficients of 100-GHz millimeter waves polarized linearly were measured for cross-cut, quarter-sawn, and
flat-sawn boards of hinoki (Chamaecyparis obtusa) that were 0.2–2.0 cm thick. This was done to examine the applicability of free-wave propagation theory for applying electromagnetic
waves to wood. It was found that the transmittance of a millimeter wave through the specimen boards was lower when the fiber
direction of a board was parallel to the direction of the electric field of the incident wave than when the fiber direction
was perpendicular to the electric field, and there was little difference in the transmittance between the tangential and radial
directions for the former case. These findings can be quantitatively explained by using propagation theory and the dielectric
properties of wood. 相似文献
35.
Haruki KITAZAWA Tomohiko INO Yasushi KAWAI Takatoshi ITOH Tadao SAITO 《Animal Science Journal》2002,73(5):395-401
The chemoattractant activity of a new chemotactic factor, 'Gasserokine' produced by Lactobacillus gasseri JCM1131T , has been proposed as a novel immunological function of probiotic lactic acid bacteria. The focus of the present study was to understand the mechanism of the chemotaxis induced by Gasserokine, using activation of an adhesion molecule, Mac-1 (CD11b/CD18) on macrophages. The macrophage chemotaxis to Gasserokine was abolished by preincubation of macrophages with the anti-Mac-1 mAb. Gasserokine induced rapid serine phosphorylation of CD18 molecules within 1 min of stimulation, but the effect was short-lived. Substantial tyrosine phosphorylation was observed in CD18-associated protein of macrophages stimulated by Gasserokine. The tyrosine phosphorylation was confirmed in macrophages stimulated with Gasserokine and also serine/threonine phosphorylation was detected on CD18 molecules by laser microscopy using a double immunostaining method. These results suggest that selective activation of intracellular signaling cascades, such as the mitogen-activated protein kinase pathway, are related to the macrophage chemotaxis induced by Gasserokine. 相似文献
36.
Tatsuhiko Matsuguchi Tadao Shimomura Sang Kyu Lee 《Soil Science and Plant Nutrition》2013,59(3):323-336
In the C2H2-C2H4 assay for measurement of heterotrophic N2 fixation in water-logged soils, the diffusion of C2H2 into the soil and the recovery of C2H4 from it are critical factors regulating the assay result. To establish an C2H2-C2H4 assay technique suitable for waterlogged soils, the C2H2-reducing activities (ARA), assayed by varying the method of assay gas filling, the pC2H2 of the assay gas, the duration of assay incubation and of soil vibration before the gas sampling, were compared. A maximum ARA was measured when the following set of procedures were applied to the soil sample in assay flasks: 1) a 4-fold repetition of I-min evacuation under 0.01 atmospheric pressure and the subsequent I-min filling under 1 atmospheric pressure with assay gas at pC2H2 of 0.1 atm, 2) an assay incubation for 3 hr, and 3) a sampling of an aliquot of the headspace gas after strongly vibrating the flask for 1 min. The ARA measured by this technique was several times larger than those measured by the techniques hitherto applied, and corresponded to an almost 80% of the V max of the sample. This technique was, therefore, proposed for the assay of heterotrophic N2 fixation in waterlogged soils. A striking depression of ARA in the soil sample prepared with agitation indicated that a microbial ecosystem established in the soil should be kept as undisturbed as possible throughout the C2H2-C2H4 assay. 相似文献
37.
Keitaro Tawaraya cMasanori Saito Mikio Morioka Tadao Wagatsuma 《Soil Science and Plant Nutrition》2013,59(3):667-671
Extract Arbuscular mycorrhizae are symbiotic associations between plant roots and soil fungi that belong to the order Glomales. They enhance the uptake of phosphate and improve plant growth (Mos.se 1973). Those functions have not fully been utilized in Japan, where a large amount of phosphate fertilizer is usually applied, because arbuscular mycorrhizal colonisation in plant is inhibited by a large application of phosphate (Mosse 1973; Abbott et al. 1984; Tawaraya et al. 1985; Schubert and Hayman 1986; Thomson et al. 1986; Amijee et al. 1989). It is important to analyse the conditions for symbiotic association between fungi and host plant to use arbuscular mycorrhiza. 相似文献
38.
Md. Akhter Hossain Chowdhury Kenji Kouno Tadao Ando 《Soil Science and Plant Nutrition》2013,59(1):175-186
The soil physicochemical characteristics and amounts of microbial biomass C, N, and S in 19 soils (10 grassland, 2 forest, and 7 arable soils) were investigated to clarify the S status in granitic regosols in Japan, in order to determine the relationships between biomass S and other soil characteristics and to estimate approximately the annual Sand N flux through the microbial biomass. Across the sites, the amount of biomass C ranged from 46 to 1,054, biomass N from 6 to 158, and biomass S from 0.81 to 13.44 mg kg-1 soil with mean values of 438.8, 85.8, and 6.15 mg kg-1 soil, respectively. Microbial biomass Nand S accounted for 3.4–7.7% and 1.1–4.0% of soil total Nand S, respectively. The biomass C: N, C : S, and N : S ratios varied considerably across the sites and ranged from 3.0–10.4, 32.5–87.7, and 5.0–18.8, respectively. Microbial biomass S was linearly related to biomass C and biomass N. The regression accounted for 96.6% for biomass C and 92.9% for biomass N of the variance in the data. The amounts of biomass C, N, and S were positively correlated with a number of soil properties, particularly with the contents of organic C, total N, SO4-S, and electrical conductivity and among themselves. The soil properties, in various linear combinations showed a variability of 84–97% in the biomass nutrients. Stepwise multiple regression indicated that biomass C, N, and S were also dependent on SO4-S as a second factor of significance which could limit microbial growth under the conditions prevailing at the study sites. Annual flux of Nand S was estimated through the biomass using the turnover rates of 0.67 for Nand 0.70 for S to be approximately 129 kg Nand 9.7 kg S ha-1 y-l, respectively, and was almost two times higher in grassland than arable soils. 相似文献
39.
Tadao Jinbo Shigeki Dan Mitsuhiro Nakaya Masakazu Ashidate Katsuyuki Hamasaki 《Fisheries Science》2013,79(3):459-467
The effect of varying levels of dietary n-3 highly unsaturated fatty acid (HUFA) content in Artemia sp. on survival, the number of days required to reach each larval stage, and the growth and morphogenesis of larvae of the horsehair crab Erimacrus isenbeckii were examined. Artemia enriched with materials of six types [condensed Nannochloropsis sp., ethyl oleate, two enrichment levels of eicosapentaenoic acid (EPA), and those of docosahexaenoic acid], and unenriched Artemia were fed to larvae. Newly hatched larvae were reared in groups of 50 individuals in two 2-l beakers until they reached the first crab stage. No significant difference was found in survival rate, growth, or morphogenesis of larvae, but fewer days tended to be required to reach the first crab stage in the group enriched with high EPA compared to the other groups. Results show that, although the amount of n-3 HUFA in unenriched Artemia might meet the demands of horsehair crab larvae for their survival, growth, and morphogenesis, Artemia should be enriched with high EPA to improve the rate of larval development. 相似文献
40.
Nakamura K Waseda K Yamamoto Y Yamada M Nakazawa M Hata E Terazaki T Enya A Imada T Imai K 《Avian diseases》2006,50(1):152-156
Cutaneous fowlpox occurring in vaccinated layer hens was investigated pathologically and microbiologically. Anorexia, decrease of egg production, increased mortality, yellow scabs on faces, and alopecia of feathered skins with yellow scabs were observed in affected hens. Histologically, proliferative and necrotic dermatitis with eosinophilic ring-shaped cytoplasmic inclusions (Bollinger bodies) and clumps of gram-positive cocci (Staphylococcus hyicus) were noted in the affected birds. Fowlpox lesions were primarily observed in the feathered skins. Proliferation of feather follicle epidermal cells, with cytoplasmic inclusions and degeneration of the feather, and bacterial clumps in the feather follicles were noted in the affected skins. Ultrastructurally, characteristic fowlpox viral particles were observed in the cytoplasmic inclusions of hyperplastic epidermal cells. Amyloid deposition was observed in the Disse space of the liver, splenic sinus, and lamina propria of the bronchiolar, bronchial, and tracheal areas. Amyloidosis could be one factor inducing the fowlpox infection in vaccinated chickens. 相似文献