Evaluation of the effects of helmet continuous positive airway pressure on laryngeal size in dogs anesthetized with propofol and fentanyl using computed tomography     

Vincenzo Rondelli  Alessandro Guarracino  Pierpaolo Iacobellis  Salvatore Grasso  Tania Stripoli  Luca Lacitignola  Edoardo Auriemma  Francesca Romano  Joaquin D. Araos  Francesco Staffieri 《Journal of Veterinary Emergency and Critical Care》2020,30(5):543-549

  相似文献   

Sidewinder gait in horses     

Monica Aleman  Emily Berryhill  Kevin Woolard  Charlotte A. Easton-Jones  Tania Kozikowski-Nicholas  Sue Dyson  Isabelle Kilcoyne 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):2122-2131

  相似文献   

Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci     

Janira Prichula  Muriel Primon-Barros  Romeu C. Z. Luz  Ícaro M. S. Castro  Thiago G. S. Paim  Maurício Tavares  Rodrigo Ligabue-Braun  Pedro A. d&#x;Azevedo  Jeverson Frazzon  Ana P. G. Frazzon  Adriana Seixas  Michael S. Gilmore 《Marine drugs》2021,19(6)

New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application.  相似文献   

Performance,nitrogen balance and microbial efficiency of beef cattle under concentrate supplementation strategies in intensive management of a tropical pasture     

Tiago Cunha Rocha  Carlos Augusto de Alencar Fontes  Renata Tavares Soares da Silva  Elizabeth Fonsêca Processi  Felipe Roberto Amaral Ferreira do Valle  Cláudio Teixeira Lombardi  Ronaldo Lopes Oliveira  Leilson Rocha Bezerra 《Tropical animal health and production》2016,48(3):673-681

  相似文献   

Remote sensing in development     

Paul CK  Mascarenhas AC 《Science (New York, N.Y.)》1981,214(4517):139-145

Remote sensing is used to acquire statistics on crops in developing countries and to locate petroleum and mineral deposits. It has increasing potential for forest monitoring and subsurface water location. Problems related to Third World use of the technology include sensitivity about the dissemination of data with high spatial resolution, exploitation by multinational companies, absorptive capacity of countries for advanced technology, autonomy in acquiring resource information, and competing foreign policy interests of the industrialized world in the global search for raw materials. The attitude of Third World countries toward use of remote sensing tends to depend on the development model they adopt.  相似文献   

Label‐free proteome of water buffalo (Bubalus bubalis) seminal plasma          下载免费PDF全文

Mayara F. Brito  Patrícia A. Auler  Guilherme C. Tavares  Cristiana P. Rezende  Gabriel M. F. Almeida  Felipe L. Pereira  Carlos A. G. Leal  Arlindo de Alencar Moura  Henrique C. P. Figueiredo  Marc Henry 《Reproduction in domestic animals》2018,53(5):1243-1246

The study aimed to describe the Bubalus bubalis seminal plasma proteome using a label‐free shotgun UDMS^E approach. A total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. Proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. In conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a foundation for further studies designed to understand regulation of sperm function and discovery of novel biomarkers for fertility. MS data are available in the ProteomeXchange with identifier PXD003728.  相似文献   

LC/ESI/MS method for the quantitative detection of guazatine residues in cereals     

Dreassi E  Zizzari AT  Zanfini A  Corbini G  Botta M 《Journal of agricultural and food chemistry》2007,55(17):6850-6856

Guazatine is a fungicide used in agriculture to control a wide range of seed-borne diseases of cereals and other vegetable foods. In this work, a LC-ESI-MS method was developed for the quantitative detection of guazatine residues in maize and hard wheat. Quantitative data were determined for the residues of the main diamines, triamines, and tetramines that cover more than 87% of the total contents of the mixture. The mean recoveries from the fortified cereals at 0.050 mg/kg ranged from 81 to 86%, with the coefficients of variation (CVs) ranging from 0.9 to 5.5% (n = 5). At 0.025 mg/kg, the recoveries ranged from 78 to 87%, with the CVs ranging from 0.8 to 6.3% (n = 5). The limits of quantification have been estimated to be 0.010, 0.004, 0.002, 0.002, 0.005, and 0.002 mg/kg, respectively, for GN, GG, GNG, GGN, GGG, and GGGG in maize and hard wheat (S/N ratio >10).  相似文献   

MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity     

Stehling O  Vashisht AA  Mascarenhas J  Jonsson ZO  Sharma T  Netz DJ  Pierik AJ  Wohlschlegel JA  Lill R 《Science (New York, N.Y.)》2012,337(6091):195-199

Instability of the nuclear genome is a hallmark of cancer and aging. MMS19 protein has been linked to maintenance of genomic integrity, but the molecular basis of this connection is unknown. Here, we identify MMS19 as a member of the cytosolic iron-sulfur protein assembly (CIA) machinery. MMS19 functions as part of the CIA targeting complex that specifically interacts with and facilitates iron-sulfur cluster insertion into apoproteins involved in methionine biosynthesis, DNA replication, DNA repair, and telomere maintenance. MMS19 thus serves as an adapter between early-acting CIA components and a subset of cellular iron-sulfur proteins. The function of MMS19 in the maturation of crucial components of DNA metabolism may explain the sensitivity of MMS19 mutants to DNA damage and the presence of extended telomeres.  相似文献   

Optimization of seasonality and mother plant nutrition for vegetative propagation of Pinus pinaster Ait     

Celia Martínez-Alonso  Angelo Kidelman  Isabel Feito  Tania Velasco  Ricardo Alía  Maria Jo?o Gaspar  Juan Majada 《New Forests》2012,43(5-6):651-663

Due to the high economic importance of Pinus pinaster Ait., there is considerable interest in developing, improving and extending the use of its families for mass clonal propagation and in breeding programmes. In the current study, we evaluated shoot growth, rooting ability and mini-cuttings production of P. pinaster in response to nitrogen fertilization and seasons. We compared eight half-sib families of P. pinaster from Asturias and Galicia (Northern Iberian Peninsula), searching for useful parameters and growing conditions to be included in a mass propagation program for clonal family forestry. We fertilized P. pinaster seedling mother plants kept in a greenhouse with three levels of nitrogen: high (HN), medium (MN) and low (LN) to evaluate rooting ability of mini-cuttings. In addition, we evaluated the maximal potential production of rooted mini-cuttings considering nine cycles of propagation over 1?year, also using three levels of nitrogen. The HN treatment significantly influenced the rooting process, with length, area and volume of roots all being positively affected. Spring was the most favourable season for mini-cuttings in the HN treatment. This study provides valuable new information to optimize the clonal propagation protocol for P. pinaster and shows that the mini-cuttings technique has great potential in mass scale cloning, providing high quality sprout production and well-formed new plants.  相似文献   

Qualitative and quantitative polymerase chain reaction (PCR) for detection of Leishmania in spleen samples from naturally infected dogs     

Solcà Mda S  Guedes CE  Nascimento EG  Oliveira GG  dos Santos WL  Fraga DB  Veras PS 《Veterinary parasitology》2012,184(2-4):133-140

Because infected dogs are widely considered to be the main domestic reservoir for Leishmania infantum (syn Leishmania chagasi) parasites in Brazil, the diagnosis of canine visceral leishmaniasis (CVL) must be made both accurately and promptly. The present study attempted to standardize a conventional polymerase chain reaction (cPCR) protocol for the detection of L. infantum DNA in canine spleen samples. Quantitative PCR (qPCR) technique was used to confirm the presence of Leishmania DNA in the canine spleen fragments. A comparison was made between the efficacies of these molecular diagnostic techniques and conventional parasitological and serological methods. cPCR protocols for spleen samples were standardized using primers that amplify a 145 bp fragment, located at the parasite kinetoplast minicircle. The genus specificity of the cPCR protocol was assessed by its inability to amplify the DNA of other common canine pathogens, such as Ehrlichia canis, Babesia canis, Toxoplasma gondii and Trypanosoma cruzi. cPCR protocol sensitivity was tested by assessing the reaction detection limit, determined to be 10 fg of L. infantum reference strain DNA, which corresponds to a range of 0.03-0.1 parasites per fragment. Standardized cPCR protocol was used to detect the presence of Leishmania in 45 dog spleen samples. Our results showed that 40% of the spleen fragment cultures were positive for Leishmania parasites, 58% of the dog serum samples tested positive using ELISA, and parasite DNA was detected in 44% using qPCR, while 47% of the spleen samples using cPCR. Diagnostic methods performance was assessed and revealed a better degree of ascertainment for cPCR when compared to other diagnostic methods. The sensitivity of ELISA was 83.3%, qPCR was 83.3%, and cPCR was 88.9%; PPV for ELISA was 57.7%, qPCR was 75% and cPCR was 76.2%; the Kappa coefficients were found to be 0.40 (fair) for ELISA, 0.64 (substantial) for qPCR and 0.68 (substantial) for cPCR. In both oligosymptomatic and polysymptomatic dogs, cPCR revealed the better performance analysis when compared to other diagnostic methods. The findings presented herein establish cPCR as the most indicated test to detect Leishmania when compared to the other two diagnostic methods evaluated. Despite the fact that the qPCR protocol provides a highly accurate quantification of parasites when targeting the SSU rRNA gene, this technique does not significantly improve the diagnosis of CVL when compared with the performance of the cPCR protocol, which focused on the kinetoplast minicircle.  相似文献