Investigation of an outbreak of sheeppox among native sheep breeds in the Western Himalayas of India     

Chahota  Rajesh  Sharma  Pardeep  Kumar  Rakesh  Gupta  Tania  Sharma  Mandeep 《Veterinary research communications》2022,46(1):101-107

Veterinary Research Communications - An outbreak of sheeppox was investigated in a cluster of villages situated in Western Himalayan ranges of a Northern Indian state. Non-migratory sheep...  相似文献   

Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon     

das Araújo  Francisca Chagas Bezerra  Moura  Elisa Ferreira  de Rodrigues  Simone Miranda  de Carvalho  Kenny Bonfim Arruda  Tavares  Eraldo José Madureira  Moura  Mônika Fecury  da Silva  Gilvan Ferreira 《Genetic Resources and Crop Evolution》2021,68(5):1809-1824

Genetic Resources and Crop Evolution - The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in...  相似文献   

The use of oral recombinant feline interferon omega in two cats with type II diabetes mellitus and concurrent feline chronic gingivostomatitis syndrome     

Rodolfo O Leal  Solange Gil  Maria TV Brito  David McGahie  Maria MRE Niza  Luís Tavares 《Irish veterinary journal》2013,66(1):19

Feline Chronic Gingivostomatitis Syndrome (FCGS) is a common disease in clinical practice. Among the therapeutic options available, long-acting corticosteroids are frequently used due to their anti-inflammatory and immunosuppressive properties. Although they may improve the clinical symptoms, they can lead to a progressive form of the disease that becomes refractory to treatment. Furthermore, their direct relationship with type II diabetes mellitus (DM) is well known. Consequently, these drugs are controversial and not recommended for routine management of FCGS. Recombinant feline interferon-omega (rFeIFN-ω) is an immunomodulatory compound. Recently, its daily oral administration has been shown to be successful in treating refractory cases of FCGS. This case study describes two clinical cases of type II DM complicated by FCGS. Both animals were calicivirus positive and they had been previously treated with long-acting corticosteroids, which may have been the major cause of DM. The two cats were treated with glargine insulin (Lantus, starting dose 1 IU/cat twice daily (BID)), achieving remission 10 and 18 weeks later respectively. Considering the difficulty with control of FCGS in these animals, an oral daily dose of rFeIFN-ω was started as an alternative to long-acting corticosteroids. In both cats oral clinical signs gradually improved and 60 days after the start of therapy the owners reported a significant relief of pain during mastication. According to the authors’ knowledge, this is the first case report that describes the successful use of rFeIFN-ω in the management of FCGS in type II diabetic cats, in which long-acting corticosteroids are contraindicated.  相似文献   

Conservation Biogeography,R.J. Ladle,R.J. Whittaker. Wiley-Blackwell,Oxford, UK (2011). 320 pp., Price £34.95, Paperback,ISBN: 978-1444335040     

Tania Escalante  Juan J. Morrone 《Biological conservation》2012,149(1):73-74

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A chimeric recombinant crustacean hyperglycemic hormone from Litopenaeus schmitti (Burkenroad) obtained as C‐terminus fusion protein boost hemolymph glucose concentration in Litopenaeus vannamei (Boone)     

Laida Ramos  Tania Rodríguez‐Ramos  Alberto Huberman  Mario Pablo Estrada 《Aquaculture Research》2013,44(7):1066-1075

To date, no hormonal treatments are available for control of shrimp reproduction and only eyestalk ablation is of practical use. The crustacean hyperglycemic hormone (CHH) is the most abundant neuropeptide of the eyestalk CHH family. It plays an important role in the regulation of hemolymph glucose levels, as its principal function, but it is also implicated in additional physiological processes such as moulting and reproduction. In the present study, the cDNA encoding Litopenaeus schmitti (Burkenroad) mature CHH was cloned into the Escherichia coli pTYB2 expression vector. Using this strategy we have obtained, for the first time, the recombinant CHH from L. schmitti with its C‐terminus fused to an intein tag. The expected fused protein of about 63 KDa was expressed in E. coli forming inclusion bodies. It was purified in a soluble form by electroelution following molecular size fractionation in sodium dodecil sulphate polyacrylamide gel electrophoresis. The ability of the chimeric CHH protein to elevate glucose levels in the hemolymph of the eyestalk‐ablated Litopenaeus vannamei (Boone) shrimps indicates that its biological activity as hyperglycemic protein is preserved. The results provide an alternative tool to obtain soluble recombinant proteins from the CHH family of neuropeptides to get a better understanding of shrimp endocrinology.  相似文献   

New record ofTrichospilus diatraeae as a parasitoid of the eucalypt defoliatorThyrinteina arnobia in Brazil     

F. F. Pereira  J. C. Zanuncio  M. T. Tavares  P. L. Pastori  G. C. Jacques  E. F. Vilela 《Phytoparasitica》2008,36(3):304-306

This is the first report ofTrichospilus diatraeae Cherian & Margabandhu, 1942 (Hymenoptera: Eulophidae) parasitizing pupae of the eucalypt defoliatorThyrinteina arnobia (Stoll, 1782) (Lepidoptera: Geometridae). Parasitized pupae of this insect were collected in the field in Brazil. In view of this finding, the potential of this parasitoid for biological control ofT. arnobia in eucalypt plantations in Brazil should be investigated. http://www.phytoparasitica.org posting June 12, 2008.  相似文献   

Potential use of Asteraceae extracts to control Spodoptera frugiperda (Lepidoptera: Noctuidae) and selectivity to their parasitoids Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) and Telenomus remus (Hymenoptera: Scelionidae)     

Wagner de Souza Tavares  Ivan Cruz  Fernando Petacci  Sebastio Loureno de Assis Júnior  Silvia de Sousa Freitas  Jos Cola Zanuncio  Jos Eduardo Serro 《Industrial Crops and Products》2009,30(3):384-388

Extracts of 12 Asteraceae were tested on Spodoptera frugiperda (Lepidoptera: Noctuidae) and on their parasitoids Telenomus remus (Hymenoptera: Scelionidae) and Trichogramma pretiosum (Hymenoptera: Trichogrammatidae). The plants Lychnophora ericoides and Trichogonia villosa were toxic for 97.7 ± 0.15% of one-day-old eggs of S. frugiperda and Lepidaploa lilacina for 72.0 ± 2.50% for two-day-old eggs of this insect. Extracts of Vernonia holosenicea, Lychnophora ramosissima and Chromolaena chaseae had higher impact on S. frugiperda, while those of Eremanthus elaeagnus and L. ericoides were more selective to T. pretiosum and T. remus. Asteraceae extracts present potential for integrated pest management programs of S. frugiperda.  相似文献   

Biochemical composition and indigestible oligosaccharides in Phaseolus vulgaris L. seeds     

da Silva Fialho L  Guimarães VM  de Barros EG  Moreira MA  Dos Santos Dias LA  de Almeida Oliveira MG  José IC  de Rezende ST 《Plant foods for human nutrition (Dordrecht, Netherlands)》2006,61(2):87-89

Common beans have a high nutritional value, but contain galactooligosaccharides (GO), which cause flatulence and intestinal discomfort in humans. The biochemical composition of ten bean cultivars was determined to select those of high protein and low GO contents. The cultivars varied in carbohydrate (47.02-60.17%), GO (3.12-5.71%), protein (22.17-33.50%), lipid (1.13-1.81%), moisture (11.42-12.93%) and ash contents (4.08-5.61%). 'Mexico 222' presented the highest alpha-galactosidase activity. Protein and GO contents were positively correlated. 'Perry Marrow' combined high protein and low GO concentrations, indicating it can be used in improvement programs aiming at high-quality cultivars for human consumption.  相似文献   

Identification and in vitro screening of avian yeasts for use as probiotic     

García-Hernández Y  Rodríguez Z  Brandão LR  Rosa CA  Nicoli JR  Elías Iglesias A  Peréz-Sanchez T  Salabarría RB  Halaihel N 《Research in veterinary science》2012,93(2):798-802

The objective of this study was to isolate and identify yeast strains from broilers excreta and to evaluate in vitro their potential for probiotic use in animal production.Methods and resultsNine yeast strains were isolated and presumptively pre-identified by biochemical assays. These isolates were grouped in six different molecular profiles using PCR-fingerprinting technique. Each profile was identified by sequencing of the D1/D2 domains of the large subunit of the 26S rRNA gene. These yeasts were identified as: Trichosporon sp. (LV-2), Wickerhamomyces anomalus (LV-6), Pichia kudriavzevii (LV-8), Kodamaea ohmeri (LV-9) and Trichosporon asahii (LV-10). A pre-screening of the strains for probiotic use was based on their ability to agglutinate pathogenic micro-organisms. These yeast strains were characterized for specific growth rate, duplication time, their cell surface hydrophobicity, medium acidification, resistance to low pH (2.0, 2.5 and 3.0) and concentrations of bile salts (0.3% and 0.6%). The isolate of W. anomalus (LV-6) had the highest agglutinating and adherence capacity, a growth rate of 2.07 × 10⁸ cfu/mL in 24 h at 30 °C, decreasing the medium pH from 6.5 to 5.23, a 25% hydrophobicity and an elevated capacity to grow under stress conditions.ConclusionsW. anomalus strain LV-6 showed the best characteristics for use as a probiotic candidate.Significance and impact of the Study:The data from this study helped in choosing a probiotic candidate from yeast to use in broiler production.  相似文献   

Effect of salt on ROS homeostasis, lipid peroxidation and antioxidant mechanisms in Pinus pinaster suspension cells     

Herlânder Azevedo  Vítor Amorim-Silva  Rui M. Tavares 《Annals of Forest Science》2009,66(2):211-211

? In the Pinus genus, information on the effectiveness of oxidative defence mechanisms during exposure to salt is lacking. The effect of salt stress imposition on ROS homeostasis was investigated using maritime pine (Pinus pinaster Ait.) suspension cells as a model system.

? Cells were maintained in MS-based medium, exposed to salt (50, 100 and 150 mM NaCl) and analysed for biomass production, evidencing a decreasing growth capacity. Use of 100 mM NaCl imposed severe salt stress without affecting cell viability, being chosen for subsequent studies on the ROS homeostasis of salt shock-treated suspension cells.

? Increased total ROS levels were evident on the second day of salt exposure, but a superoxide ion transient burst was immediately noticeable. Additionally, lipid peroxide formation seemed to correlate with superoxide ion breakdown. In-gel superoxide dismutase activity evidenced a FeSOD homodimer with strongly increasing activity between hours 12–48 of salt stress imposition. Subsequently, P. pinaster Fe-Sod1 and csApx1 genes were isolated from a cDNA library and expression was shown to increase within 12–24 h.

? Results show that severe salt treatment generates oxidative stress in P. pinaster cells despite the induction of antioxidant systems, and suggest a putative involvement of ROS in salt stress signalling.

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