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41.
The persistence of foot-and-mouth disease virus on wool 总被引:1,自引:0,他引:1
SUMMARY Five Suffolk sheep, held in a high-security isolation room, were exposed for 2 hours to the aerosol of 3 mature pigs that had been infected with foot-and-mouth disease virus (FMDV), strain O1-BFS. The fleeces of 3 of the sheep were contaminated with FMDV at 2 days post exposure (dpe), while at 5 dpe the fleeces of all 5 sheep were more extensively, and more heavily, contaminated. The persistence of FMDV on contaminated wool was examined in vitro using multiple 0.5 g samples of Merino wool that were each contaminated with one of 3 strains of FMDV in tissue-culture medium: O1-BFS, O-Morocco (O-MOR 9/91) or an Asia 1 strain (TAI 1/90). Wool samples were held at either 4°C, 18°C or 37°C, and decay curves were established for each virus at each temperature. These curves predicted that O1-BFS, O-MOR 9/91 and TAI 1/90 would fall below detect-able levels at 72, 70 and 48 days post contamination (pc), respectively, for wool stored at 4°C; at 11, 12 and 12 days pc, respectively, for wool stored at 18°C; and at 57, 68 and 33 hours pc, respectively, for wool stored at 37°C. For wool contaminated with O1-BFS-infected sheep faeces, urine or blood, or with O1-BFS-infected cattle saliva, decay curves predicted virus to persist for 5 to 11 days pc at 18°C. We demonstrated that the simulated scouring of FMDV-contaminated wool at 60° to 70°C would usually reduce virus to below detectable levels. The detergent component of the scouring process had little, if any, antiviral activity, and scouring at 20°C or 50°C had limited impact on FMDV titres . We recommend that either (1) simple storage of FMDV-contaminated wool for 4 weeks at temperatures of 18°C or higher, or (2) scouring of contaminated wool at 60° to 70°C would be sufficient to remove the threat of FMDV-contaminated wool being infectious to other animals . 相似文献
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Flavor release in the presence of melanoidins prepared from L-(+)-ascorbic acid and amino acids 总被引:1,自引:0,他引:1
Obretenov C Demyttenaere J Tehrani KA Adams A Kersiene M De Kimpe N 《Journal of agricultural and food chemistry》2002,50(15):4244-4250
High-molecular-weight (HMW) water-soluble melanoidins were prepared from model systems of L-(+)-ascorbic acid-glycine, L-(+)-ascorbic acid-lysine, L-(+)-ascorbic acid-glutamic acid, and glucose-glycine using a very recently approved standard protocol. The amount of HMW water-soluble melanoidins prepared from L-(+)-ascorbic acid was over 5-15 times higher than the amount obtained from glucose. The study of the release of a model flavor compound, namely isoamyl acetate, from melanoidins by solid-phase microextraction (SPME) showed that SPME is a suitable technique for the analysis of flavor release from melanoidin-containing solutions. From the studies on the retention capacity of the melanoidins toward isoamyl acetate, an increased release of the flavor compound was observed from the melanoidins prepared from the L-(+)-ascorbic acid-glycine model system, whereas the opposite effect was observed from the melanoidins prepared from the L-(+)-ascorbic acid-lysine/glutamic acid model systems. The melanoidins prepared from the glucose-glycine model system had the same effect as the melanoidins prepared from the L-(+)-ascorbic acid-glycine model system. 相似文献
44.
NW TOMKINS NN JONSSON MP YOUNG AN GORDON KA McCOLL 《Australian veterinary journal》1997,75(10):722-723
On the basis of clinical signs and histological findings eight 9-month-old male rusa deer ( Cervus timorensis ) were diagnosed with sheep associated-malignant catarrhal fever. Following a variable course involving rectal temperatures around 40.5°C, depression, inappetence, diarrhoea, corneal opacity and hypopyon all animals died or were euthanased over a 5-week period. Severe multifocal vasculitis, mainly periglomerular and in the arcuate vessels were consistent histological findings which in the past have been adequate to confirm clinical diagnosis of sheep associated-malignant catarrhal fever. A nested poly-merase chain reaction test has been used to detect a sheep associated-malignant catarrhal fever PRC product, 238 base-pairs in size, in DNA extracted from lymphocyte preparations. The result supported the diagnosis of sheep associated-malignant catarrhal fever in these deer. 相似文献
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Magnetic Field and Plasma Observations at Mars: Initial Results of the Mars Global Surveyor Mission 总被引:2,自引:0,他引:2
MH Acu?a JEP Connerney P Wasilewski RP Lin KA Anderson CW Carlson J McFadden DW Curtis D Mitchell H Reme C Mazelle JA Sauvaud C d'Uston A Cros JL Medale SJ Bauer P Cloutier M Mayhew D Winterhalter NF Ness 《Science (New York, N.Y.)》1998,279(5357):1676-1680
The magnetometer and electron reflectometer investigation (MAG/ER) on the Mars Global Surveyor spacecraft has obtained magnetic field and plasma observations throughout the near-Mars environment, from beyond the influence of Mars to just above the surface (at an altitude of approximately 100 kilometers). The solar wind interaction with Mars is in many ways similar to that at Venus and at an active comet, that is, primarily an ionospheric-atmospheric interaction. No significant planetary magnetic field of global scale has been detected to date (<2 x 10(21) Gauss-cubic centimeter), but here the discovery of multiple magnetic anomalies of small spatial scale in the crust of Mars is reported. 相似文献
48.
Linking winter and summer events in a migratory bird by using stable-carbon isotopes 总被引:1,自引:0,他引:1
For migratory birds, early arrival and physical condition on the breeding grounds are important determinants of reproductive success and fitness. Differences in arrival times often exceed a month, and later arriving individuals are often in poorer condition. Habitat-specific isotopic signatures indicate that the quality of winter habitats occupied by American redstarts (Setophaga ruticilla) determines their physical condition and spring departure dates, which in turn result in variable arrival schedules and condition on temperate breeding grounds. These findings link events in tropical winter grounds with those in temperate breeding areas for a migratory songbird and provide evidence that winter habitats may be limiting. 相似文献
49.
IL NIELSEN KA JACOBS PJ HUNTINGTON† CB CHAPMAN‡ KC LLOYD§ 《Australian veterinary journal》1988,65(6):181-185
Adverse reactions to intramuscular injections of procaine penicillin G are reported in 11 horses, five of which died. The clinical findings are presented and suggest central nervous involvement in most cases. Post mortem findings in one horse were consistent with anaphylaxis whereas in other cases the clinical findings, duration of treatment, speed of onset and subsequent completion of treatment supports diagnosis of an acute procaine toxicity syndrome. 相似文献
50.