Molecular and serological detection of Babesia spp. in neotropical and exotic carnivores in Brazilian zoos     

André MR  Adania CH  Teixeira RH  Allegretti SM  Machado RZ 《Journal of zoo and wildlife medicine》2011,42(1):139-143

Large and small piroplasms have been observed in the blood smears of various wild carnivores, but few studies utilizing molecular characterization have been done. The goal of this present study was to investigate the presence of Babesia sp. by molecular and serologic techniques in exotic and neotropical carnivores maintained in captivity at Brazilian zoos. Blood and sera samples were collected from 146 Brazilian wild felids, 21 exotic felids, 1 genet (Genetta tigrina), 3 European wolves (Canis lupus), and 94 Brazilian wild canids in Brazilian zoos in the S?o Paulo and Mato Grosso states and in the Federal District. A total of 53 wild felids (31.74%) and 10 wild canids (10.31%) were seropositive for Babesia canis by Indirect Fluorescent Antibody Test (IFAT). Antibodies were detected in ocelots, little-spotted cats, margays, pampas cats, jaguars, pumas, jaguarundis, crab-eating foxes, and bush dogs. Babesia sp. DNA, with high similarity to B. leo, was detected in one pampas cat and one genet.  相似文献   

Clavibacter michiganensis subsp. michiganesis: Tracking strains using their genetic differentiations by ISSR markers in Southern Turkey     

Ömür Baysal  Francesco Mercati  Hatice İkten  Raziye Çetinkaya Yıldız  Francesco Carimi  Yeşim Aysan  Jaime A. Teixeira da Silva 《Physiological and Molecular Plant Pathology》2011,75(3):113-119

There is no available data published related to the dissemination of bacterial canker caused by Clavibacter michiganensis subsp. michiganensis (Cmm) and its genetic diversity in Turkey. It is important to control new introduced inoculum sources by commercial seeds and seedlings. Pathogens were identified by morphological features and the identity was confirmed by PCR amplification using a specific primer PSA-4, PSA-R in addition to microbiological tests. ISSR markers showing high polymorphism ratios were selected and used to characterize Cmm strains. The collected strains were classified into different groups on the basis of ISSR-PCR fingerprints, which showed remarkable genetic specificity and diversity not previously identified in Cmm, suggesting that genetic differences are related to dissemination of the pathogen in the region. This is the first ever study carried out on the characterization of Cmm using ISSR. The selected ISSR primers to characterize Cmm can be used to determine genetic differences in further studies.  相似文献   

Measurement and prediction of nitrogen loss by simulated erosion events on cultivated forest soils of contrasting structure     

P.C. Teixeira  R.K. Misra   《Soil & Tillage Research》2005,83(2):204-217

The objectives of this study were to determine nitrogen (N) loss associated with erosion of forest soils and to explore the role of soil structure and other factors governing N enrichment of sediment to aid prediction of N loss. We measured erosion, size distribution of aggregates in the sediment and N distribution in various aggregate fractions using simulated rainfall on samples of three cultivated forest soils of contrasting structure (repacked in trays) exposed to four erosion conditions. Both sediment loss and N loss increased with slope and kinetic energy of rainfall suggesting greater dependency of N loss on sediment loss than on N concentration in the sediment. Irrespective of erosion treatments and soil type, the bulk sediment and its size fractions were mostly richer in N than those of the uneroded soils. The enrichment ratio (E_R) and concentration ratio (C_R) of N for sediment cast some doubt on the application of raindrop stripping as a mechanism of N enrichment of sediment for soils of widely differing characteristics. Previously published models of N enrichment of sediment did not predict E_R for our soils satisfactorily. However, an empirical model using published data on erosion and N loss agreed well with the results. This predictive method only requires information on sediment loss that can be easily obtained from an erosion model.  相似文献   

Genetic Relationships and Variation among Biotypes of Dallisgrass (Paspalum dilatatum Poir.) and Related Species Using Random Amplified Polymorphic DNA Markers     

Rogéria Beatriz Miz  Tatiana Teixeira de Souza-Chies 《Genetic Resources and Crop Evolution》2006,53(3):541-552

The genus Paspalum L. consists of more than 400 species. Around twenty-five informal groups of species are recognized in Paspalum and the Dilatata group is of special interest because its members are excellent potential forage grasses. Seventy-five germplasm accessions, representing 15 taxa, were analyzed using randomly amplified polymorphic DNA (RAPD). Polymorphisms were observed with twenty-two primers in the Dilatata group and 16 of those were analyzed. Four hundred and four different RAPD fragments were generated, resulting in an average of 25.2 bands per primer. Among the 404 markers analyzed, 48 (11.88%) were exclusive for the P. dilatatum Poir. biotypes, 31 (7.67%) were exclusive to taxa belonging to other groups included in this study, 28 markers (6.93%) were diagnosed for other species of the Dilatata group and 16 (3.96%), for natural hybrids. Extensive RAPD variation was found among the species studied. Inter- and intra-taxonomic polymorphisms were detected. A dendrogram based on the RAPD data shows some clusters corresponding to the same taxa. However, the biotypes of P. dilatatum do not form a cluster. The present work confirms that the RAPD technique can be used to determine genetic relationships between the taxa belonging to the Dilatata group.  相似文献   

Fenthion poisoning of native birds in southern Queensland     

RA McKENZIE  AEL LANHAM  JD TAYLOR  JA GIBSON  RJ PIERCE 《Australian veterinary journal》1996,74(4):321-323

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A bio-economic modelling project for small-scale milk production systems in South-East Brasil     

N.R. Brockington  C.A. Gonzalez  J.M. Veil  R.R. Vera  N.M. Teixeira  A.G. de Assis 《Agricultural Systems》1983,12(1):37-60

This paper reports on the development and use of a bioeconomic model to simulate dairy enterprises in the Zona da Mata of Minas Gerais State in south-east Brasil.In view of the relatively small size of the units, each animal within the herd is accounted for on an individual basis and the main biological events and processes are treated stochastically. Within the limits of given herdranges for genetic potentials, actual rates of performance are estimated from the levels of feeding and other management factors. An energy balance approach is used to assess the contributions of pasture, cut forage and concentrates in the diet.The model has been used to help in identifying priority areas for applied research, to explore various combinations of herd potential and level of feeding, the behaviour of different herd sizes and the potential returns from specific technical innovations.  相似文献   

Artificial Insemination in Dogs     

JA Rodger 《Australian veterinary journal》1993,70(12):462-463

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Chronic renal failure and urolithiasis in a 2-years-old colt     

JA LAING  AL RAISIS  RJ RAWLINSON  AC SMALL 《Australian veterinary journal》1992,69(8):199-200

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4 THE EFFECTS OF EXERCISE AND INJURY ON CHONDROITIN SULFATE AND KERATAN SULFATE CONTENT IN EQUINE SYNOVIAL FLUID.     

MP  Brown  AHK Plaas  JD Sandy  JA Hernandez  KA Merritt  . 《Veterinary surgery : VS》2003,32(6):599-599

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Antimicrobial susceptibility of Pasteurella multocida isolates from cases of pneumonia in slaughter swine from south-east Queensland     

CP STEPHENS  JA GIBSON  L RICHARDSON 《Australian veterinary journal》1995,72(4):156-156

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