Estimation of the size of the genome ofTaylorella equigenitalis by crossed-field gel electrophoresis     

M. Matsuda  Y. Asami  T. Miyazawa  T. Sugawara  M. Kumano  Y. Isayama  M. Honda 《Veterinary research communications》1994,18(2):99-102

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Effect of PCB-126 on intracellular accumulation and transepithelial transport of vinblastine in LLC-PK1 and its transformant cells expressing human P-glycoprotein     

Sasawatari S  Toki M  Horie T  Nakano Y  Ikeda T  Ueda K  Fujise H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1079-1085

The effects of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB-126), which is the most toxic congener of coplanar polychlorinated biphenyls (Co-PCBs), on intracellular accumulation and transepithelial transport of vinblastine were examined in porcine kidney cells, LLC-PK1, and its transformant cells expressing human P-glycoprotein (LLC-MDR1). The accumulation decreased less than one-tenth in LLC-MDR1 compared to LLC-PK1. In both cells, the accumulation increased with the addition of PCB-126 and cyclosporine A (CYA), which are P-glycoprotein modulators, though the magnitudes were different in these two cell groups as well as for these two chemicals. Thus, PCB-126 might inhibit extrusion of vinblastine through the drug extrusion system as does CYA. In both the cells, there might be an endogenous drug extrusion system other than P-glycoprotein that was inhibited by CYA or PCB-126. The net basal-to-apical transepithelial transport of vinblastine increased 1.7-fold more in LLC-MDR1 than in LLC-PK1. By adding PCB-126 on the apical side, the transport was greatly decreased by -76% in the monolayer of both cells. By adding PCB-126 and CYA on the basal side in LLC-MDR1 monolayer, the transports increased -1.7-fold, so that PCB-126 might inhibit the extrusion of vinblastine on both the apical and basal sides. One of the causes to be considered for the adverse effects of Co-PCBs, in addition to the binding with an aryl hydrocarbon receptor, might be the modification of drug transport by its interaction with the drug transport system.  相似文献   

Single intradermal tuberculin tests of milking buffaloes and cows in Nepal     

Pun MB  Prasai TP  Dhakal M  Jha VK  Shrestha KB  Jha VC  Sato T  Morita Y  Kozawa K  Kimura H 《The Veterinary record》2004,154(4):124

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Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development          下载免费PDF全文

Akiyoshi Egashira  Nobuhiko Yamauchi  Md. Rashedul Islam  Kazuki Yamagami  Asami Tanaka  Hikaru Suyama  El‐Sharawy Mohamed El‐Sayed  Shoji Tabata  Takashi Kuramoto 《Animal Science Journal》2016,87(8):1048-1054

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst.  相似文献   

Overwintering of brown leaf spot fungus, Mycochaetophora gentianae, in infected gentian leaves as the primary inoculum source   总被引：1，自引：0，他引：1  

Syuuichi Nekoduka  Kazuaki Tanaka  Teruo Sano 《Journal of General Plant Pathology》2013,79(3):175-177

Overwintering of the brown leaf spot fungus, Mycochaetophora gentianae, in infected gentian leaves was studied in Iwate, northern Japan. Sporophores were produced on overwintered, infected leaves when they were sampled from January to July, but not in August after incubation in high humidity at 15 °C. Symptoms developed on gentian plants grown in soil artificially infested with overwintered, infected leaves that were either left throughout the experiments or removed before planting. Few lesions developed when plants were grown in soil infested with conidia. These results indicate that M. gentianae can overwinter in infected leaves, which act as the primary inoculum source.  相似文献   

Mechanism underlying potato spindle tuber viroid affecting tomato (Solanum lycopersicum): loss of control over reactive oxygen species production     

Fujibayashi  Misato  Suzuki  Takahiro  Sano  Teruo 《Journal of General Plant Pathology》2021,87(4):226-235

Journal of General Plant Pathology - Severe infection of the tomato cultivar ‘Rutgers’ with potato spindle tuber viroid (PSTVd) variants resulted in systemic stunting, leaf...  相似文献   

Leaf spot of barnyardgrass caused by Exserohilum oryzicola in Japan and the fungal influence on rice,barley, bread wheat,durum wheat,and soybean     

Tomioka  Keisuke  Asami  Hidenori  Chiba  Masahiro  Kobayashi  Hidekazu  Nagata  Kenji  Mori  Shinsuke  Yamazaki  Ryo  Kawasaki  Yohei  Yasumoto  Satoko  Masunaka  Akira  Sekiguchi  Hiroyuki  Ishikawa  Naoyuki 《Journal of General Plant Pathology》2021,87(5):281-286

Journal of General Plant Pathology - Brown lesions were found on leaves of the weed barnyardgrass in paddy fields in Hiroshima Prefecture in western Japan in June 2017. A fungus, isolated...  相似文献   

Effect of an automatic milking system on milking traits     

Takashi HAYASHI  Teruo KAWAMURA 《Animal Science Journal》2002,73(5):403-408

Recent studies in Japan of the effect of automatic milking systems (AMS) on the behavior of dairy cows have confirmed the labor-saving aspect of AMS, but have not solved some of the problems affecting milking traits. The influence of AMS on milking was examined in 39 Holstein cows at Iwate Agricultural Research Center from June 1998 to December 1999, by measuring and recording the yield per milking, milking finish time, milking frequency, and daily yield of milking. Four milking traits were analyzed separately as the objective variable in a linear model, with parity, days in milking, month after installation of the AMS as independent variables. Primiparous cows milked more frequently in the middle and late stage of lactation, yielded less per milking and less per day in the early stage of lactation than multiparous cows with the AMS. Lower frequencies were observed at noon and midnight for cleaning of the bulk tank. By contrast, higher milking frequency in the forenoon was shown. The yield per milking rose and declined, following a normal lactation curve. However, milking frequency rose slowly, and declined gradually in the late stage of lactation. After installing a one-way gate, the cows milked more frequently, with a lower yield per milking and a greater daily milk yield, than before installation.  相似文献   

Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry,strawberry, and corn grown using conventional,organic, and sustainable agricultural practices   总被引：10，自引：0，他引：10  

Asami DK  Hong YJ  Barrett DM  Mitchell AE 《Journal of agricultural and food chemistry》2003,51(5):1237-1241

Secondary phenolic metabolites play an important role in plant defense mechanisms, and increasing evidence indicates that many are important in human health. To date, few studies have investigated the impact of various agricultural practices on levels of secondary plant metabolites. To address this issue, the total phenolic (TP) content of marionberries, strawberries, and corn grown by sustainable, organic, or conventional cultural practices were measured. Additionally, the effects of three common postharvest processing treatments (freezing, freeze-drying, and air-drying) on the TP content of these agricultural products were also investigated. Statistically higher levels of TPs were consistently found in organically and sustainably grown foods as compared to those produced by conventional agricultural practices. In all samples, freeze-drying preserved higher levels of TPs in comparison with air-drying.  相似文献   

Chemical Characteristics in Stemflow of Japanese Cedar in Japan     

Asami Nakanishi  Hideaki Shibata  Yoji Inokura  Toshio Nakao  Hiroto Toda  Fuyuki Satoh  Kaichiro Sasa 《Water, air, and soil pollution》2001,130(1-4):709-714

To clarify the characteristics in stemflow of Japanese cedar (Cryptomeria japonica), we conducted the annual and extensive observation. We examined the chemistry of bulk and wet deposition, throughfall and stemflow at 26 forested sites in June and September 1998. The each sampling site was broadly distributed in all over the Japanese archipelago. The stemflow pH of Japanese cedar was significantly lower (p<0.01) than precipitation and stemflow of broad-leaved species in both months. There were significant anion deficits in stemflow of Japanese cedar, suggesting that organic anions derived from plant sources play an important role in the stemflow acidity. Our results suggested that the strong stemflow acidity in Japanese cedar was derived from an internal biological characteristic rather than influences of external acidic deposition.  相似文献