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Genetic analysis and PCR-based identification of major <Emphasis Type="Italic">Fusarium</Emphasis> species causing head blight on wheat in Japan 总被引：1，自引：1，他引：0
Wen-Hsin Chung Hideo Ishii Kumiko Nishimura Michiyo Ohshima Toshitaka Iwama Hideaki Yoshimatsu 《Journal of General Plant Pathology》2008,74(5):364-374
Identifying the Fusarium species cause Fusarium head blight (FHB) and produces mycotoxins in wheat and other cereal is difficult and time consuming because of confusing
phenotypic classification systems. In Japan, the F. graminearum complex, F. culmorum, F. avenaceum, and Microdochium nivale predominantly cause FHB. The internal transcribed spacer (ITS) and 5.8S of rDNA, a partial sequence of β-tubulin and mitochondrial
cytochrome b (cytb) genes of the four species were PCR-amplified and analyzed. On the basis of the ITS, β-tubulin and cytb sequences, F. avenaceum and M. nivale are distinct from the F. graminearum complex and F. culmorum, whereas the F. graminearum complex is closely related to F. culmorum. Moreover, thiophanate–methyl-resistant isolates of the F. graminearum complex and F. culmorum did not have an amino acid substitution at amino acid codon 198 or 200 of β-tubulin. In contrast, very highly or highly thiophanate–methyl-resistant
isolates of M. nivale had Glu (GAG) substituted with Ala (GCG) or Lys (AAG) at codon 198, respectively. The allele-specific PCR assay was used
to identify the F. graminearum complex and F. culmorum, and these Fusarium species could be distinguished rapidly. 相似文献
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In order to develop a model for predicting the period of male flower anthesis in Cryptomeria japonica, it is requisite to clarify the rest break process in male flowers. In this study, the effect of alternating temperatures
in a daily cycle on rest break was investigated using twigs bearing male flowers from three clones of C. japonica. Four temperature treatments with the same daily mean of 8°C were applied to sample twigs over a period of 35 days: (1) 8°C
constant (TR-A); (2) 7°–10°C alternating (TR-B); (3) 5.5°–13°C alternating (TR-C); and (4) 4°–16°C alternating (TR-D). After
treatment, the sample twigs were forced to bloom at a constant temperature of 16°C, and the cumulative effective temperatures
(CETs) required for anthesis were compared. While the CETs for anthesis were the same for TR-A and TR-B, that for TR-C was
significantly higher. After TR-D, almost no male flower bloomed, indicating that this treatment was ineffective in breaking
rest. When considering these results on an hour basis, it was a reasonable interpretation that 13°C had no effect on breaking
rest while the temperatures below 10°C were all equally effective. On the other hand, temperatures of 16°C or above were considered
to have an antagonizing effect that cancels the effects of chilling temperatures below 10°C. These results suggest that the
effects of high temperatures during the daytime in chilling periods should be properly incorporated into a model of the process
by which rest of male flowers is broken. 相似文献
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Mutations in the acetolactate synthase genes of sulfonylurea-resistant biotypes of Lindernia spp. 总被引：1，自引：0，他引：1
Acetolactate synthase (ALS) is a key enzyme in the biosynthetic pathway of branched-chain amino acids. A mutation of the ALS gene causing amino acid substitution at the position of proline in Domain A makes ALS less sensitive to sulfonylureas, which are ALS-inhibiting herbicides. We cloned partial ALS genes from four Lindernia plants, L . dubia var. dubia , L . dubia var. major , L . micrantha and L . procumbens , for which biotypes resistant to sulfonylureas have been found in paddy fields. The clones were classified into two groups in each Lindernia plant: Als1 and Als2 . Sequencing of the clones and alignment of deduced amino acid sequences with previously reported ALS of other species suggested that the cloned region contains an intron in both Als1 and Als2 . Comparison of Als1 between resistant and susceptible biotypes showed that the proline of Domain A was replaced by alanine, serine or glutamine in all resistant biotypes of Lindernia plants, while it was conserved in all susceptible biotypes. This amino acid substitution in ALS encoded by Als1 is involved in the resistant mechanism of ALS to sulfonylurea in the four Lindernia plants. 相似文献
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Takuhito Nozoe Akira Uchino Shigenori Okawa Shuichi Yoshida Yukihide Kanda Yukinori Nakayama 《Soil Science and Plant Nutrition》2013,59(2):200-205
Laboratory experiments were conducted to investigate (1) the effects of the addition of rice (Oryza sativa. L.) bran to paddy soil on the germination of Monochoria vaginalis (Burm. f.) Kunth, and (2) the relationship between the electric conductivity (EC) of the soil solution and germination. Soil samples were collected at 4 sites in Japan. After flooded soils with rice bran had been incubated for 7?d at 30°C, the soil solution was collected using a porous cup and the EC of the soil solution was measured. The amounts of rice bran added to the soil were 0%, 0.3%, 0.6% and 0.9% (weight(w)/w). In the soil solution obtained, seeds of M. vaginalis were incubated for 3?d at 30°C, and the germination percentage was then analyzed. The addition of rice bran suppressed germination, and the degree of suppression increased with increasing content of rice bran. Although the same amount of rice bran was applied to each soil, the degree of growth suppression by rice bran as well as the EC of the soil solution differed among the soils. In each soil, there was a positive correlation between the amount of rice bran and EC, and the degree of growth suppression significantly increased with an increase in EC. When EC was higher than 150?mS?m?1, seeds of M. vaginalis hardly germinated. There was no significant correlation between the oxidation-reduction potential (Eh) of soil and seed germination, suggesting that EC is a more reliable and convenient indicator than Eh for evaluating the relationship between the addition of organic material and seed germination. In conclusion, the addition of rice bran to soil increases the EC of the soil solution, and EC is one of the factors that suppress the germination of M. vaginalis. The suppressive effect of rice bran on germination is different among soils. This fact is attributed to the difference in EC due to the addition of rice bran. Thus, it is expected that EC can be used as an indicator for determining how much rice bran to add. 相似文献
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Koyama H Yoshii H Yabu H Kumada H Fukuda K Mitani S Rousselot JF Hirose H Uchino T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1107-1111
Comparison of the QT interval and corrected QT interval values that were calculated by the methods of Bazett (QTc1) and Fridericia (QTc2) were made between dogs with or without cardiac diseases to determine the influence of the QT interval on canine heart failure. Upon comparison of the measured values on ECG between the cardiac disease and non-cardiac disease groups, it was observed that the heart rate(HR) was significantly higher in the cardiac disease group than in the non-cardiac disease group, although the QT interval was similar in the two groups. The QTc1 and QTc2 were significantly longer in the cardiac disease group than in the non-cardiac disease group. With the progression of the New York Heart Association Class, the HR tended to increase. The QTc1 and QTc2 became significantly prolonged with the progression of heart failure. Nevertheless, because Bazett's correction formula is known to overcorrect when the HR is high, it was considered that the QTc1 was actually overcorrected by high HR with the progression of heart failure. The QTc2, on the other hand, was only slightly influenced by HR, suggesting that the prolongation was due to the progression of heart failure. These results suggest that the prolongation of QTc2 in cardiac disease reflects the substantial prolongation of the QT interval without the influence of HR. It is suggested that the QTc2 could be a useful parameter for assessing the degree of heart failure in dogs with cardiac disease. 相似文献
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Kazuya Sato Masaki Okamoto Yuta Suzuki Kagehisa Kiba Hiroki Tatsumi Hisashi Teramura Toshitaka Ikeuchi 《Fisheries Science》2013,79(6):943-948
The transactivational property of natural and synthetic chemicals via medaka GR1b was investigated after development of a stable cell line for the reporter gene assay. In our study, cortisol was the most potent agonist among the natural corticoids assayed for potency [EC50 (concentration of agonist provoking a response halfway between the baseline and maximum response) 68 nM] and efficacy. Three artificial corticosteroids, namely, dexamethasone [EC50 16 nM, relative agonistic activity to cortisol (RAA) 144 %], prednisolone (EC50 81 nM, RAA 116 %) and clobetasol propionate (EC50 0.10 nM, RAA 220 %), showed strong agonistic activity and were more potent than the original corticoid, F. All synthetic corticoids used in our study were full agonists. Interestingly, melengestrol acetate, a synthetic progestogen, induced luciferase activity in a dose-dependent manner. Based on its EC50 value and RAA of 29 nM and 57 %, respectively, this molecule was assessed as a partial agonist. None of the other steroids and chemicals assayed in our study induced an agonistic response. In conclusion, we successfully developed a stable reporter gene assay that can be used to assess the transactivational property of glucocorticoid-like chemicals toward medaka GR1b. 相似文献
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Yield losses of soybean and maize by competition with interseeded cover crops and weeds in organic-based cropping systems 总被引：1，自引：0，他引：1
Weed management is a major issue in organic farming systems. Although interseeding cover crops is one alternative to herbicides, cover crops often suppress not only weeds but also main crops. Therefore, using cover crops for weed control without adverse effects on main crop growth is important. To verify the effect of cover crops on competition between main crops, cover crops and weeds in a snowy-cold region, main crops soybean (Glycine max Merr.) in 2005 and maize (Zea mays L.) in 2006 were grown with cover crops winter rye (Secale cereale L.) and hairy vetch (Vicia villosa Roth), respectively. The cover crops were sown on three sowing dates: before main crop planting (Pre-MC), on the same date of main crop planting (Syn-MC) and after main crop planting (Post-MC). A plot without cover crops (Sole-MC) was used as a control. The dry weight (DW), vegetation cover ratio (VCR), vertical community structure and chlorophyll content were measured to estimate the competition between main crops, cover crops and weeds. Weed DW was suppressed significantly by sowing cover crops in both soybean and maize. This weed suppression was associated with the increase of VCR of main crops plus cover crops at the early growth stage of main crops. Soil seed banks of dominant weed also became lower by sowing cover crops, implying the importance of proper weed management for suppressing weeds successively. In addition, the sowing dates of cover crops had large effects on main crops DW especially in maize, i.e., it was significantly lower in Pre-MC and Syn-MC than in Post-MC. Although the cover crop height was markedly shorter than the main crop height, the chlorophyll content of the main crops was significantly lower when cover crops were sown earlier. These results suggest that the growth inhibition of main crops by cover crops was partly caused by competition for nutrients between main crops and cover crops, and this growth inhibition was alleviated when cover crops were sown after the establishment of main crops. Consequently, soybean yield was the highest in Post-MC and decreased by 29%, 18% and 7% in Sole-MC, Pre-MC and Syn-MC, respectively, and maize yield was also the highest in Post-MC and decreased by 68%, 100% and 24% in Sole-MC, Pre-MC and Syn-MC, respectively. It was concluded that weeds could be controlled effectively by sowing cover crops after planting main crops in organic farming systems in a snowy-cold region. 相似文献
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