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A micromethod employing the neutralisation and direct immunofluorescence technique for the detection of antibodies against swine fever in pig serum samples is described. The micromethod is simple and reproducible when compared with the macromethod. A total of 80 blood samples were randomly collected for comparison. The results are promising and reveal a 100 per cent correlation with the macrotechnique. 相似文献
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Abstract Extract As dairy herds get larger cows have to walk further, and foot care becomes more important. Lameness from bruising of the soles of the feet is a particular hazard (Tranter and Morris 1991, especially on hard tracks with loose angular stones or grit (Clackson and Ward 1991. One way of reducing this problem might be to provide an overlay of woodchips on top of the normal hard track. This would provide a softer grit-free surface, which could be more comfortable for the cows. 相似文献
14.
不同旱稻品种灌浆期抗旱生理适应性的研究 总被引:3,自引:0,他引:3
选择抗旱性不同的4个旱稻品种,对其灌浆期旗叶抗旱性生理指标进行测定。结果表明:超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及游离脯氨酸含量均为抗旱性较强品种(旱稻15号,旱稻2号)极显著地高于抗旱性较弱品种(旱稻1号,旱稻3号);丙二醛(MDA)含量和细胞膜相对透性大小表现为抗旱性较强品种(旱稻15号,旱稻2号)极显著地低于抗旱性较弱品种(旱稻1号,旱稻3号)。SOD、POD、CAT活性和丙二醛含量、游离脯氨酸含量、细胞膜相对透性可用来说明旱稻品种之间的抗旱生理适应性的差异。 相似文献
15.
Nikoletta Ntalli Nikolaos Monokrousos Christos Rumbos Dorothea Kontea Despoina Zioga Maria D. Argyropoulou Urania Menkissoglu-Spiroudi Nikolaos G. Tsiropoulos 《Journal of pest science》2018,91(1):29-40
The need for environmentally friendly agricultural practices has led to the development of plant-based nematicides for root-knot nematode control. The efficacy of these botanicals has been tested primarily under laboratory and rarely under actual field conditions. Moreover, any side effects on non-target soil organisms that support soil biological activity are usually ignored. Herein, we evaluate the efficacy of Melia azedarach preparations against Meloidogyne spp. in a tomato greenhouse, by root gall examination and soil J2 enumeration. We also assessed side effects on soil microbes through PLFA analysis and microbivorous nematodes, and we quantified several plant growth parameters (e.g., fruit number and weight, root weight). Different treatments within the greenhouse included M. azedarach ripe fruit powder (MFP), ripe fruit water extract (MWE) and furfural, one of the principal active ingredients of M. azedarach and previously known to exhibit fumigant nematicidal activity. Results were compared to those obtained with the commercial nematicide oxamyl (Vydate® 10 SL) and an untreated control. All treatments were repeated every 20 days throughout the cultivation period. MFP and MWE suppressed Meloidogyne spp. often at the same levels obtained by furfural and oxamyl treatments and enhanced soil biological activity, as indicated by the proliferation of soil microbes and microbial feeding nematodes. Furfural and oxamyl adversely affected the soil community, especially the free-living nematodes. Moreover, furfural was phytotoxic to tomato plants in spite of its natural origin. 相似文献
16.
Ioannis?GravalosEmail author Anastasios?Georgiadis Dimitrios?Kateris Theodoros?Gialamas Evagelos?Bompolas Zisis?Tsiropoulos Avgoustinos?Avgoustis Panagiotis?Xyradakis 《Precision Agriculture》2017,18(1):19-36
This research is a study on the relationship of irrigation water treatments and soil moisture distribution uniformity (DU). Soil moisture distribution was based on long-term data sets that were collected using a novel electromagnetic sensor-based platform moving inside sub-surface horizontal access-tubes. The research was conducted under controlled laboratory conditions in a soil tank. The irrigation treatments regarding two case studies under dry and wet soil conditions (from permanent wilting point to field capacity) were conducted for a period of 115 and 110 days respectively. In dry soil conditions, the irrigation water treatments strongly affect the DU of soil moisture that can be achieved constantly using non-uniform irrigation treatments. In contrast, the DU of soil moisture in wet soil conditions was maintained at a high percentage and was slightly affected by irrigation treatments. However, obtaining accurate soil moisture information at a large scale over a long period can be used to improve water use efficiency. 相似文献
17.
An established sward of binary mixtures of meadow fescue (Festuca pratensis) and white clover (Trifolium repens) (either AberHerald, Grasslands Huia or Sandra) was subjected to (A) no further defoliation, (B) a defoliation in late September or (C) a defoliation in late October after four harvests had been taken during the grazing season. About a tonne of dry matter (DM) was removed by the autumn defoliations. There were two levels of nitrogen application in spring, either 0 or 90 kg ha?1. The development of grass and clover morphology and population sizes from early autumn until the first harvest the following year was followed by regular sampling of the above-ground material. Stolons were analysed for total non-structural carbohydrates (TNCs), and the temperature at stolon level was continuously recorded. There were no interactions between autumn defoliation, clover cultivar or nitrogen treatments on any of the parameters studied. White clover growing-point numbers and stolon morphological characteristics were reduced in size during the winter and did not recover during the spring. A defoliation in late September resulted in the greatest reduction, whereas there were no differences between the other two treatments. The grass tiller population increased from early autumn until the last sampling occasion in May, but both autumn defoliations resulted in a smaller increase. Defoliation in late September had the greatest impact. The TNC content of white clover stolons fell from about 350 g kg?1 to 150 g kg?1 DM from late autumn until late April. There were small differences between the treatments, but a defoliation in late September resulted in a significantly lower level in late autumn. The temperature amplitude at stolon level was consistently greater in plots defoliated in late September. Total DM harvested in spring was 4367, 2564 and 3536 kg ha?1, of which 388, 352 and 460 kg ha?1 was white clover, from treatments A, B and C respectively. It is concluded that an autumn defoliation may affect the overwintering of white clover negatively, but that the effect on the grass may be even more detrimental. 相似文献
18.
玉米蛋白粉替代鱼粉对异育银鲫生长、蛋白酶活性及表观消化率的影响 总被引:3,自引:0,他引:3
分别用4%、8%、12%和16%玉米蛋白粉替代异育银鲫饲料中25%、50%、75%和100%鱼粉配制4种等氮、等能的试验饲料,以不替代鱼粉的饲料为对照,试验饲料与对照饲料等氮、等能,在室内循环水养殖系统饲养异育银鲫(初始体重1.32±0.16 g)71 d,旨在研究玉米蛋白粉替代鱼粉对异育银鲫生长、蛋白酶活性以及表观消化率的影响.结果表明,玉米蛋白替代鱼粉对异育银鲫的存活率、肥满度无显著性影响(P>0.05).4%、8%及12%玉米蛋白粉组的相对增重率、相对增长率、饲料系数、蛋白质效率、肠道和肝胰脏蛋白酶活性、干物质和蛋白质表现消化率与对照组无显著差异(P>0.05),玉米蛋白粉完全替代鱼粉组的生长性能、饲料利用、蛋白酶活性和表观消化率均显著低于对照组(P<0.05).因此,本实验条件下,玉米蛋白粉可以替代75%鱼粉. 相似文献
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20.