Describing the sticking phenomenon of aminoplastic resins: introduction of a new test method     

Alexandra Himsel  Johann Moser  Wolfgang Kantner  Roland Mitter  Jürgen Gießwein  Hendrikus W. G. van Herwijnen  Ulrich Müller 《Wood Science and Technology》2015,49(4):681-694

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Homogeneous shear stress field of wood in an Arcan shear test configuration measured by means of electronic speckle pattern interferometry: description of the test setup     

Müller  Ulrich  Ringhofer  Andreas  Brandner  Reinhard  Schickhofer  Gerhard 《Wood Science and Technology》2015,49(6):1123-1136

Wood Science and Technology - Several studies have dealt with the problem of how to measure the shear modulus of small clear wood specimens, avoiding bias from normal compression, bending, tension...  相似文献   

Differential Activity of Carboxylic Acid Amide Fungicides Against Various Developmental Stages of Phytophthora infestans     

Cohen Y  Gisi U 《Phytopathology》2007,97(10):1274-1283

ABSTRACT Three carboxylic acid amide (CAA) fungicides, mandipropamid (MPD), dimethomorph (DMM) and iprovalicarb (IPRO) were examined for their effects on various asexual developmental stages of Phytophthora infestans in vitro and in planta. Germination of cystospores and direct germination of sporangia were inhibited with nanomole concentrations of MPD (0.005 mug/ml) and micromole concentrations of DMM (0.05 mug/ml) or IPRO (0.5 mug/ml). A temporary exposure of 1 h to CAAs was not detrimental to germination and infectivity of sporangia or cystospores. CAAs applied to cystospores at 1 h after the onset of germination did not prevent the emergence of germ tubes, but inhibited their further growth and deformed their shape. None of the fungicides affected discharge of zoospores from sporangia or the encystment (cell wall formation/assembly) of the zoospores. Mycelium growth in solid or liquid media was inhibited with micromole concentrations. CAAs mixed with sporangia and drop inoculated onto detached leaves strongly suppressed infection. Curative application at 1 day postinoculation (dpi) required higher concentrations of CAAs than preventive application to inhibit infection and lost its effectiveness at 2 dpi. When sprayed on established late blight lesions 4 days after inoculation, CAAs reduced sporangial production in a dose-dependent manner. Trans-laminar protection of potato or tomato leaves, although achieved with higher doses, was more effective with MPD than with DMM or IPRO. Shade house studies demonstrated superior control of late blight epidemics by MPD compared with the other molecules. The data suggest that germ tube formation by cystospores or sporangia is the most sensitive stage in the life cycle of P. infestans to CAAs. Of the three CAAs, MPD had the highest intrinsic activity against spore germination. This property, together with its better trans-laminar activity, makes MPD more effective than DMM or IPRO in controlling epidemics caused by P. infestans.  相似文献   

After nasopharyngeal infection,foot-and-mouth disease virus serotype A RNA is shed in bovine milk without associated mastitis     

Marcel Suchowski  Michael Eschbaumer  Jens P. Teifke  Reiner Ulrich 《Journal of veterinary diagnostic investigation》2021,33(5):997

Foot-and-mouth disease (FMD) is a highly contagious aphthoviral infection of cloven-hoofed animals, inducing vesiculopustular stomatitis, pododermatitis, and thelitis. Vesicular fluid represents a major pathway of virus excretion, but bovine milk is another important source of virus shedding. We describe here the time course of FMD virus (FMDV) excretion in the milk and characterize associated lesions in the mammary gland. Three dairy cows were infected by nasopharyngeal instillation of FMDV and monitored over 12 d. Autopsy was performed at the end of the study, and specimens were collected for histopathology, IHC, and RT-qPCR. All 3 cows developed fever, drooling, vesiculopustular stomatitis, interdigital dermatitis, and thelitis. FMDV RNA was detectable in whole milk until the end of the trial, but only transiently in saliva, nasal secretions, and blood serum. Although histology confirmed vesiculopustular lesions in the oral and epidermal specimens, the mammary glands did not have unequivocal evidence of FMDV-induced inflammation. FMDV antigen was detectable in skin and oral mucosa, but not in the mammary gland, and FMDV RNA was detectable in 9 of 29 samples of squamous epithelia but only in 1 of 12 samples of mammary gland.  相似文献   

Impact of temperature and nutrition on the toxicity of the insecticide λ-cyhalothrin in full-lifecycle tests with the target mosquito species Aedes albopictus and Culex pipiens     

Aljoscha Kreß  Ulrich Kuch  Jörg Oehlmann  Ruth Müller 《Journal of pest science》2014,87(4):739-750

The global spread of the Asian tiger mosquito Aedes albopictus, an urban pest as well as a vector for arboviruses, is a threat for public health. As control measures include the use of insecticides such as the pyrethroid λ-cyhalothrin, it is crucial to assess their efficiency and their potential impact on the biodiversity especially under climate change conditions. To evaluate the environmental risk, biotests are well established for non-target organisms but not yet for mosquitoes. We therefore developed a full-lifecycle biotest for mosquitoes kept under quarantine conditions based on the OECD guideline 219. Therewith we tested the effect of temperature and nutrition on the ecotoxicological response to λ-cyhalothrin on the mosquitoes Ae. albopictus and Culex pipiens by assessing sublethal and life history parameters. The efficiency of λ-cyhalothrin decreased in both mosquito species with increasing temperature and changed with feeding protocol. At effective concentrations for potential mosquito control in surface waters, λ-cyhalothrin poses a high risk for indigenous aquatic key role species inhabiting the same microhabitats. Those aspects should to be taken into account in vector control strategies.  相似文献   

Habitat structure and prey aggregation determine the functional response in a soil predator-prey interaction     

Olivera Vucic-Pestic  Björn C. Rall  Ulrich Brose 《Pedobiologia》2010,53(5):307-5

Functional responses describe the per capita consumption rates of predators depending on prey density, which quantifies the energy transfer between trophic levels. We studied a typical interaction of the litter-soil systems between hunting spiders (Pardosa lugubris; Araneae: Lycosidae) and springtails (Heteromurus nitidus; Collembola: Entomobryidae) at varying habitat structure, i.e. with moss vs. without moss. We found a hyperbolic increase in consumption (functional response type II) in the treatment without habitat structure that was converted into a roller-coaster (or dome-shaped in a broad sense) functional response in treatments with habitat structure. Additional experiments suggest that the reduced per capita consumption rates at high prey densities may be explained by aggregative defence behaviour of the springtails. Experimentally, this behaviour was induced by the presence of habitat structure. We analyzed the net-energy gain of this predator-prey interaction by comparing the predator’s metabolic energy loss to its energy gain by consumption. In treatments with habitat structure, the net-energy gain of the predator was limited at intermediate prey densities where prey aggregation reduced the consumption rates. Our results stress the importance of habitat structure and prey behaviour in shaping the functional response in a typical soil-litter predator-prey interaction.  相似文献   

Erstellung eines Moorstandortkataloges als eine grundlage für das moorschutzprogramm in mecklenburg‐vorpommern     

Ulrich Ratzke 《Archives of Agronomy and Soil Science》2013,59(3):187-206

Der Moorstandortkatalog Mecklenburg‐Vorpommern wird als Bestandteil der bodenkundlichen Landesaufnahme seit 1992 durch den Geologischen Dienst erarbeitet. Moore nehmen 12% der Wirtschaftsfläche, entsprechend etwa 300000 ha ein. Fast ausschließlich sind es Niedermoore (nur ca. 5 000 ha Regenmoore) verschiedener Typen unter subkontinentalen Klimabedingungen und größtenteils landwirtschaftlicher Nutzung.

Gegenwärtig sind 30% aller Niedermoorböden, es handelt sich um die größten, mit kartographischen Übersichten, Daten und fachspezifischen Erläuterungen über ihren Aufbau und Zustand katalogisiert. Damit liegt eine wichtige Grundlage für das in Ausarbeitung befindliche Landes‐Moorschutzprogramm vor. Die Daten für jedes Moorgebiet werden im Fachinformationssystem Boden gespeichert. Die auf die Erstellung des Moorstandortkataloges gerichteten Untersuchungen zeigen, daß sich die ursprüngliche Genese der Niedermoore den lithogenetischen und morphologischen Bedingungen ihres Wassereinzugsgebietes anglich. Die Zuordnung der hydrologisch‐genetischen Moortypen folgt weitgehend der geomorphologischen Struktur des Jungmoränengebietes, z.B. Durchströmungsmoore in der ebenen bis welligen Grundmoräne, Küstenüberflutungsmoore am ca. 314km langen Küstenabschnitt der südlichen Ostsee, Kesselmoore in Endmoränengebieten usw.  相似文献   

News item     

Hady Farag  Ulrich Hommel 《Euphytica》1991,59(1):97-97

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Efficient analysis of egg yolk proteins and their thermal sensitivity using sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing and nonreducing conditions     

Guilmineau F  Krause I  Kulozik U 《Journal of agricultural and food chemistry》2005,53(24):9329-9336

The multiple functional properties of egg yolk are mostly influenced by its complex protein composition. The high lipid content of egg yolk as well as the low solubility of delipidated egg yolk lipoproteins make analysis by conventional chromatographic or electrophoretic techniques a difficult task. This work describes a method to profile egg yolk proteins after delipidation with acetone using sodium dodecyl sulfate polyacrylamide gel electrophoresis on precast 8-18% T polyacrylamide gradient gels. Twenty bands were obtained for the whole egg yolk profile with molecular weights ranging between 5 and 221 kDa. The bands were identified based on their molecular weight and by comparison with isolated egg yolk subfractions. The dissociation behavior under reducing and nonreducing conditions provided additionally helpful information for identification and characterization of the yolk proteins. The method presented is very well suited for assaying the thermal sensitivity of whole yolk and its components and thus for the characterization of heat treatment processes.  相似文献   

Distortion of genetically modified organism quantification in processed foods: influence of particle size compositions and heat-induced DNA degradation     

Moreano F  Busch U  Engel KH 《Journal of agricultural and food chemistry》2005,53(26):9971-9979

Milling fractions from conventional and transgenic corn were prepared at laboratory scale and used to study the influence of sample composition and heat-induced DNA degradation on the relative quantification of genetically modified organisms (GMO) in food products. Particle size distributions of the obtained fractions (coarse grits, regular grits, meal, and flour) were characterized using a laser diffraction system. The application of two DNA isolation protocols revealed a strong correlation between the degree of comminution of the milling fractions and the DNA yield in the extracts. Mixtures of milling fractions from conventional and transgenic material (1%) were prepared and analyzed via real-time polymerase chain reaction. Accurate quantification of the adjusted GMO content was only possible in mixtures containing conventional and transgenic material in the form of analogous milling fractions, whereas mixtures of fractions exhibiting different particle size distributions delivered significantly over- and underestimated GMO contents depending on their compositions. The process of heat-induced nucleic acid degradation was followed by applying two established quantitative assays showing differences between the lengths of the recombinant and reference target sequences (A, deltal(A) = -25 bp; B, deltal(B) = +16 bp; values related to the amplicon length of the reference gene). Data obtained by the application of method A resulted in underestimated recoveries of GMO contents in the samples of heat-treated products, reflecting the favored degradation of the longer target sequence used for the detection of the transgene. In contrast, data yielded by the application of method B resulted in increasingly overestimated recoveries of GMO contents. The results show how commonly used food technological processes may lead to distortions in the results of quantitative GMO analyses.  相似文献