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The annual timing of temperate forest leaf colouring is affected by climate change; to date, its modelling remains a challenge. We take advantage of a ca. 400 leaf colouring observations database acquired in France during the period of 1997–2006 in order to develop a new modelling framework aimed at predicting the spatial and year-to-year variability of leaf colouring in European beech and oak (Fagus sylvatica L., Quercus petraea (Matt.) Liebl. and Quercus robur L.). We postulate colouring to be the outcome of a one-way process triggered by photoperiod and progressing through a photoperiod-sensitive cold-degree day summation procedure. Observations were pooled according to genus for the fitting and ensuing validation procedures. Parameters of the model suggest that colouring processes start earlier, and are sensitive to higher temperatures for Quercus than for Fagus. Errors associated with the modelled predictions are up to 13.0 days in Fagus and 10.3 days in Quercus, which are significantly lower than errors associated with (1) the overall mean (null model) or (2) previously published modelling frameworks. When averaged on a site basis, model predictions reproduced spatial variability of leaf colouring over the French territory with good efficiency (modelling efficiencies: 0.44 for Fagus, 0.45 for Quercus). The interannual variability of leaf colouring over France was fairly reproduced (r2 = 0.74 for Fagus, 0.83 for Quercus). On that basis, we claim that the modelling framework developed herein can be efficiently integrated into more general schemes aimed at simulating matter and energy fluxes on a regional scale, and we provide a generic parameterisation of the model to be integrated into such schemes. When used in a prospective analysis, the model predicts a trend towards delay in leaf colouring of 1.4 and 1.7 days per decade in Fagus and Quercus, respectively, over the period of 1951–2099 in France.  相似文献   
94.
The therapeutic value of antibiotics depends on the susceptibility of the infecting microorganism and the pharmacological profile of the drugs. To assess the value of an antibiotic combination of polymyxin B and miconazole this study examined the in vitro synergistic potential of the two drugs on Gram-negative and Gram-positive bacteria and yeast. Antifungal and antibacterial activity was tested by minimum inhibitory concentration (MIC) of broth macrodilution and urea broth microdilution, by fluorescence microscopy and flow cytometry. Synergism was calculated using the fractional inhibitory concentration index (FICi). With Staphylococcus intermedius as target we found up to an eightfold reduction of the individual MICs when both drugs were combined. However, the FICi was 0.63 suggesting no real interaction between the two drugs. With Escherichia coli, Pseudomonas aeruginosa, and Malassezia pachydermatis as targets the antimicrobial drug combination reduced the MICs of polymyxin B and miconazole from fourfold to hundredfold resulting in FICi between 0.06 and 0.5 which defines a synergistic action. Thus, if polymyxin B and miconazole are combined their effect is greater than the sum of the effects observed with polymyxin B and miconazole independently, revealing bactericidal and fungicidal synergism. Our results indicate a strong therapeutic value for the combination of these antimicrobial agents against Gram-negative bacteria and yeast and a weaker value against Gram positive bacteria for clinical situations where these pathogens are involved.  相似文献   
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Short‐rotation forestry (SRF) on arable soils has high potentials for biomass production and leads to long‐term no‐tillage management. In the present study, the vertical distributions of soil chemical and microbial properties after 15 y of SRF with willows and poplar (Salix and Populus spp.) in 3‐ and 6‐year rotations on an arable soil were measured and compared to a pertinent tilled arable site. Two transects at different positions in the relief (upper and lower slope; transect 1 and 2) were investigated. Short‐rotation forestry caused significant changes in the vertical distribution of all investigated soil properties (organic and microbial C, total and microbial N, soil enzyme activities), however, the dimension and location (horizons) of significant effects varied. The rotation periods affected the vertical distribution of the soil properties within the SRF significantly. In transect 1, SRF had higher organic‐C concentrations in the subsoil (Bv horizon), whereas in transect 2, the organic‐C concentrations were increased predominantly in the topsoil (Ah horizon). Sufficient plant supply of P and K in combination with decreased concentrations of these elements in the subsoil under SRF pointed to an effective nutrient mobilization and transfer from the deeper soil horizons even in the long term. In transect 1, the microbial‐C concentrations were higher in the B and C horizons and in transect 2 in the A horizons under SRF than under arable use. The activities of β‐glucosidases and acid phosphatases in the soil were predominantly lower under SRF than under arable use in the topsoil and subsoil. We conclude, that long‐term SRF on arable sites can contribute to increased C sequestration and changes in the vertical distribution of soil microbial biomass and soil enzyme activities in the topsoil and also in the subsoil.  相似文献   
98.
  • ? Two decades after the launching of the monitoring program of forest ecosystems in Europe (ICP forests), a unique data set is now available regarding the foliar nutritional status of the main broad-leaved species growing in much-diversified sites (soil, climate).
  • ? This study focuses on the foliar concentration time series (1993 to 2005) of the French (RENECOFOR), Walloon and Luxemburg broad-leaved plots. The aim is to show long-term trends while taking the inter-plot and inter-annual variability into account.
  • ? Two kinds of statistical processing were used to analyze the data on foliar chemistry: principal component analysis (PCAs) and linear mixed models. In general, the main temporal trends reveal a decrease in the foliar P concentration and a decrease in the foliar Ca concentration, except for European beech on acid soils.
  • ? These temporal patterns confirm the trends previously observed and could be explained by the joint actions of several processes that influence tree nutrition in the long term: tree age, N and S deposition, harvesting and climate.
  •   相似文献   
    99.
    Biotrophic pathogens, such as the related maize pathogenic fungi Ustilago maydis and Sporisorium reilianum, establish an intimate relationship with their hosts by secreting protein effectors. Because secreted effectors interacting with plant proteins should rapidly evolve, we identified variable genomic regions by sequencing the genome of S. reilianum and comparing it with the U. maydis genome. We detected 43 regions of low sequence conservation in otherwise well-conserved syntenic genomes. These regions primarily encode secreted effectors and include previously identified virulence clusters. By deletion analysis in U. maydis, we demonstrate a role in virulence for four previously unknown diversity regions. This highlights the power of comparative genomics of closely related species for identification of virulence determinants.  相似文献   
    100.
    For two Scots pine (Pinus sylvestris) ecosystems in S Germany with different atmospheric N deposition (Pfaffenwinkel, intermediate N deposition; Pustert, large N deposition), the supply with phosphorus (P) has been monitored for unfertilized and fertilized plots over more than four decades by foliar analysis (1964–2007). Additionally, topsoil concentrations and stocks of total P and plant-available P (citric-acid-extractable phosphate) were quantified in 10-year intervals (1982/1984, 1994, 2004). At both sites, fertilization experiments, including the variants control, NPKMgCa + lime, PKMgCa + lime + introduction of lupine, corresponding to an addition of 75 and 90 kg ha−1 P in Pustert and Pfaffenwinkel, respectively had been established in 1964. Our study revealed different trends of the P nutritional status for the pines at the two sites during the recent four decades: At Pustert, elevated atmospheric N deposition together with small topsoil P pools resulted in significant deterioration of Scots pine P nutrition and in an increasingly unbalanced N/P nutrition. At Pfaffenwinkel a trend of improved P nutrition from 1964 to 1991 was replaced by an opposite trend in the most recent 15 years. For our study sites, which are characterized by acidic soils with thick O layers, the forest floor stock of citric-acid-extractable phosphate showed a strong and significant correlation with the P concentration in current-year pine foliage, and thus was an appropriate variable to predict the P nutritional status of the stands. Total P stocks as well as the concentrations of total P in the forest floor or in the mineral topsoil were poorly correlated with pine foliar P concentrations and thus inappropriate predictors of P nutrition. P fertilization in the 1960s sustainably improved the P nutritional status of the stands. At Pfaffenwinkel, foliar P concentrations and topsoil stocks of citric-acid-extractable phosphate were increased at the fertilized plots relative to the control plots even 40 years after fertilization; at Pustert, foliar P concentrations were increased for about 20 years.  相似文献   
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