The influence of aluminium on the structural organization of the roots of barley in culture <Emphasis Type="Italic">in Vitro</Emphasis>     

E. N. Baranova  A. V. Bakulina  I. G. Shirokikh  O. N. Shuplechova  I. A. Chaban  A. A. Gulevich  V. Y. Polyakov 《Russian Agricultural Sciences》2011,37(5):358-363

The structural organization of roots, induced in the callus cultures of barley genotypes differing in aluminum tolerance: cv Kupetz, cv 999-93 and its somaclonal form R 999-93 was studied. The goal of this research was to determine how the toxic effects of aluminum at low pH affects the structure of the root and any cells damage of different tissues meristematic zone. At pH 5.8–6.0 root callus from different genotypes had specific features, manifested in the structure root cap, the epidermis, the form of roots. In the presence of 40 mg/1 Al³⁺ ions and pH 3.7–3.8 in the roots, callus was obtained from the cv Kupetz, there is strong damage to the epidermis, root cap and some other tissues, which, although and resulted in the death of cells, however, were not lethal character. In roots, callus derived from varieties 999-93, under the influence Al³⁺ ions was observed irreversible changes that lead either to compaction cytoplasm and nuclei, especially in the central part of the root or to the plasmolysis and the destruction of cells, especially in the meristematic zone. On transverse sections was found damaged cells of the central part of the root, formation of the densified outer cell wall of epidermal cells. At the investigation of R 999-93 callus roots it was be shown that most of the cells of meristematic zone of root retained their viability at influence of Al3+ ions. So, the somaclon from cv 999-93, obtained earlier in callus culture by selection on Al-tolerance, differs from the original form at the level of mesostructure.  相似文献   

Species proportions by area in mixtures of Scots pine (<Emphasis Type="Italic">Pinus sylvestris</Emphasis> L.) and European beech (<Emphasis Type="Italic">Fagus sylvatica</Emphasis> L.)     

Gerald Dirnberger  Hubert Sterba  Sonia Condés  Christian Ammer  Peter Annighöfer  Admir Avdagić  Kamil Bielak  Gediminas Brazaitis  Lluís Coll  Michael Heym  Václav Hurt  Viktor Kurylyak  Renzo Motta  Maciej Pach  Quentin Ponette  Ricardo Ruiz-Peinado  Jerzy Skrzyszewski  Vít Šrámek  Géraud de Streel  Miroslav Svoboda  Tzvetan Zlatanov  Hans Pretzsch 《European Journal of Forest Research》2017,136(1):171-183

Scots pine (Pinus sylvestris L.) and European beech (Fagus sylvatica L.) dominate many of the European forest stands. Also, mixtures of European beech and Scots pine more or less occur over all European countries, but have been scarcely investigated. The area occupied by each species is of high relevance, especially for growth evaluation and comparison of different species in mixed and monospecific stands. Thus, we studied different methods to describe species proportions and their definition as proportion by area. 25 triplets consisting of mixed and monospecific stands were established across Europe ranging from Lithuania to Spain in northern to southern direction and from Bulgaria to Belgium in eastern to western direction. On stand level, the conclusive method for estimating the species proportion as a fraction of the stand area relates the observed density (tree number or basal area) to its potential. This stand-level estimation makes use of the potential from comparable neighboring monospecific stands or from maximum density lines derived from other data, e.g. forest inventories or permanent observations plots. At tree level, the fraction of the stand area occupied by a species can be derived from the proportions of their crown projection area or of their leaf area. The estimates of the potentials obtained from neighboring monospecific stands, especially in older stands, were poorer than those from the maximum density line depending on the Martonne aridity index. Therefore, the stand-level method in combination with the Martonne aridity index for potential densities can be highly recommended. The species’ proportions estimated with this method are best approximated by the proportions of the species’ leaf areas. In forest practice, the most commonly applied method is an ocular estimation of the proportions by crown projection area. Even though the proportions of pine were calculated here by measuring crown projection areas in the field, we found this method to underestimate the proportion by 25% compared to the stand-level approach.  相似文献   

Nature-inspired soluble iron-rich humic compounds: new look at the structure and properties     

Tatiana A. Sorkina  Alexander Yu. Polyakov  Natalia A. Kulikova  Anastasia E. Goldt  Olga I. Philippova  Alina A. Aseeva  Alexey A. Veligzhanin  Yan V. Zubavichus  Denis A. Pankratov  Eugene A. Goodilin  Irina V. Perminova 《Journal of Soils and Sediments》2014,14(2):261-268

Purpose

Humic substances (HS) being natural polyelectrolyte macromolecules with complex and disordered molecular structures are a key component of the terrestrial ecosystem. They have remarkable influence on environmental behavior of iron, the essential nutrient for plants. They might be considered as environmental friendly iron deficiency correctors free of synthetic iron (III) chelates disadvantages. The main goal of this study was to obtain water-soluble iron-rich humic compounds (IRHCs) and to evaluate their efficiency as chlorosis correctors.

Materials and methods

A facile preparation technique of IRHCs based on low-cost and available parent material was developed. The iron-containing precursor (ferrous sulfate) was added dropwisely into alkaline potassium humate solution under vigorous stirring and pH-control. A detailed characterization both of organic and inorganic parts of the compounds was provided, the iron species identification was carried out jointly by EXAFS and Mössbauer spectroscopy. Bioassay experiments were performed using cucumber Cucumis sativus L. as target plants. Plants were grown in modified Hoagland nutrient solution, prepared on deionized water and containing iron in the form of IRHCs. Total iron content in dry plants measured by spectrophotometry after oxidative digestion and the chlorophyll a content determined after acetone extraction from fresh plant were used as parameters illustrating plants functional status under iron deficiency condition.

Results and discussion

The high solubility (up to130 g/l) and iron content (about 11 wt%) of the IRHCs obtained allow considering them to be perspective for practical applications. A set of analytical methods has shown that the main iron species in IRHCs are finely dispersed iron (III) oxide and hydroxide nanoparticles. An application of the precursor solution acidification allows to obtain compounds containing a significant part of total iron (up to 30 %) in the form of partly disordered iron (II–III) hydroxysulphate green rust GR(SO₄ ^2?). Bioavailability of iron from IRHCs was demonstrated using bioassay in cucumber plants grown up on hydroponics under iron deficiency conditions.

Conclusions

The application of iron oxides chemistry for humic substance containing solution was proved to be an effective approach to synthesis of IRHCs. Using bioassay on cucumber plants C. sativus L. under iron deficiency conditions, the efficiency of compounds obtained as chlorosis correctors was demonstrated. Application of water-soluble IRHCs led to significant increase of chlorophyll a content (up to 415 % of the blank) and iron content in plants (up to 364 % of the blank) grown up on hydroponics.  相似文献   

Minute virus as a possible cause of neurological problems in dogs     

Eminaga S  Palus V  Cherubini GB 《The Veterinary record》2011,168(4):111-112

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Temporal changes in clinical and radiographic variables in dogs with preclinical myxomatous mitral valve disease: The EPIC study     

Adrian Boswood  Sonya G. Gordon  Jens Häggström  Martin Vanselow  Gerhard Wess  Rebecca L. Stepien  Mark A. Oyama  Bruce W. Keene  John Bonagura  Kristin A. MacDonald  Mark Patteson  Sarah Smith  Philip R. Fox  Karen Sanderson  Richard Woolley  Viktor Szatmári  Pierre Menaut  Whitney M. Church  M. Lynne O'Sullivan  Jean-Philippe Jaudon  Jan-Gerd Kresken  John Rush  Kirstie A. Barrett  Steven L. Rosenthal  Ashley B. Saunders  Ingrid Ljungvall  Michael Deinert  Eric Bomassi  Amara H. Estrada  Maria J. Fernandez Del Palacio  N. Sydney Moise  Jonathan A. Abbott  Yoko Fujii  Alan Spier  Michael W. Luethy  Roberto A. Santilli  Masami Uechi  Anna Tidholm  Christoph Schummer  Philip Watson 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(3):1108-1118

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LETTERS     

Viktor Szatmari  DVM 《Veterinary radiology & ultrasound》2003,44(4):486-487

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Simultaneous congenital and acquired extrahepatic portosystemic shunts in two dogs.     

Viktor Szatmári 《Veterinary radiology & ultrasound》2003,44(4):486-7; author reply 487

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IMAGING DIAGNOSIS — MAGNETIC RESONANCE IMAGING OF INTRACRANIAL INFLAMMATORY FIBROSARCOMA IN A MIXED BREED DOG          下载免费PDF全文

Elena Scarpante  Viktor Palus  Brian Alan Summers  Abby Caine  Giunio Bruto Cherubini 《Veterinary radiology & ultrasound》2016,57(1):E5-E9

An 8‐year‐old mixed‐breed dog presented with progressive behavioral changes and altered mentation. Magnetic resonance imaging (MRI) of the brain revealed an olfactory and frontal lobe extra‐axial mass. The mass exhibited the following MRI signal intensity characteristics: T2W mixed, T1W iso‐ to hypointense, FLAIR hyperintense, and strong contrast enhancement. The mass was removed with cavitronic ultrasonic surgical aspirator (CUSA) assisted neurosurgery. Based on histopathological appearance and immunohistochemistry, the diagnosis of inflammatory fibrosarcoma was made. To our knowledge, this is the first report describing MRI characteristics of intracranial inflammatory fibrosarcoma in the veterinary literature.  相似文献   

MRI features of CNS lymphoma in dogs and cats     

Palus V  Volk HA  Lamb CR  Targett MP  Cherubini GB 《Veterinary radiology & ultrasound》2012,53(1):44-49

The magnetic resonance (MR) imaging features of central nervous system lymphoma in eight dogs and four cats are described. Intracranial lesions affected the rostrotentorial structures in six dogs and caudotentorial structures in two cats. Lesions affected the spinal cord in two dogs and in two cats. One dog and one cat with intracranial lymphoma had signs of local extracranial extension and lymphadenopathy. Lesions were considered extraparenchymal in four dogs and three cats, intraparenchymal in two dogs and one cat, and appeared to have both intra- and extraparenchymal components in two dogs. All lesions were hyperintense in T2-weighted images when compared to white matter, most were hypointense in T1-weighted images (7/12), and most were hyperintense in fluid-attenuated inversion recovery (FLAIR) images (5/9). When compared to grey matter, these lesions appear either isointense (5/12) or hyperintense (7/12) on T2-weighted images, half of them were hypointense in T1-weighted images (6/12), and most were isointense in FLAIR images (7/9). Lesion margins were usually indistinct in T2-weighted images (10/12) and had perilesional hyperintensity in FLAIR images (7/9). The majority of lesions (10/12) had abnormal meninges around the lesion and half (6/12) had generalized contrast enhancement. Mass effect was evident in all lesions. Although not specific, when combined with the history and neurologic signs, MR features aid presumptive diagnosis that should be confirmed by cytology or histopathology.  相似文献   

Typhlitis and abdominal cystic lymphangiomatosis in a Mt. Carmel blind mole rat (Nannospalax (ehrenbergi) carmeli)     

Sós E  Molnár V  Gál J  Németh A  Perge E  Lajos Z  Csorba G 《Journal of zoo and wildlife medicine》2012,43(2):416-420

An abdominal cystic lymphangiomatosis in a Mt. Carmel blind mole rat (Nannospalax (ehrenbergi) carmeli) is described. This case was most likely due to a congenital abnormality with long-term compensation by the animal. The case describes the clinical course and subsequent postmortem examination. The death in the animal was caused by an abscess in the peritoneal wall and subsequent peritonitis.  相似文献