排序方式： 共有60条查询结果，搜索用时 281 毫秒
31.
Oliveira CB Da Silva AS Vargas LB Bitencourt PE Souza VC Costa MM Leal CA Moretto MB Leal DB Lopes ST Monteiro SG 《Veterinary parasitology》2011,175(1-2):9-14
Pigeons (Columba livia) cohabit with humans in urban and rural areas, representing a public health problem since microorganisms are transmitted through the inhalation of dust from their dry feces (chlamydiosis) and through ingestion of their undercooked or poorly refrigerated meat (toxoplasmosis). This study aimed to evaluate the presence of Chlamydophila psittaci and Toxoplasma gondii in pigeons from four cities in São Paulo State, Brazil. C. psittaci was evaluated through hemi-nested polymerase chain reaction (hnPCR) using cloacal and tracheal swabs, whereas T. gondii specific antibodies were assessed by means of modified agglutination test (MAT), mouse brain and muscle bioassay, and polymerase chain reaction (PCR). To confirm the infection in mice, T. gondii antibodies were assayed by using indirect fluorescent antibody test (IFAT). Considering C. psittaci, 40/238 (16.8%; 95%CI 12.6–22.1%) samples were positive according to hnPCR, especially for the cities of São Paulo (42.5%) and Bauru (35%). As regards T. gondii, 12/238 (5%; 95%CI 2.9–8.6%) serum samples were positive according to MAT. Of these, five samples had titer equal to 1:8; six samples, 1:16; and one sample, 1:32. Bioassay, IFAT and PCR were negative for mouse toxoplasmosis. The absence of T. gondii antibodies suggests that pigeons may be infected with a low concentration of the agent, not detected by the antigen test. Thus, C. psittaci represents an actual problem concerning bird health. 相似文献
32.
Jaenicke-Després V Buckler ES Smith BD Gilbert MT Cooper A Doebley J Pääbo S 《Science (New York, N.Y.)》2003,302(5648):1206-1208
Maize was domesticated from teosinte, a wild grass, by approximately 6300 years ago in Mexico. After initial domestication, early farmers continued to select for advantageous morphological and biochemical traits in this important crop. However, the timing and sequence of character selection are, thus far, known only for morphological features discernible in corn cobs. We have analyzed three genes involved in the control of plant architecture, storage protein synthesis, and starch production from archaeological maize samples from Mexico and the southwestern United States. The results reveal that the alleles typical of contemporary maize were present in Mexican maize by 4400 years ago. However, as recently as 2000 years ago, allelic selection at one of the genes may not yet have been complete. 相似文献
33.
34.
Viviane Saporiti Juliana T. T. Fritzen Cesar Feronato Raquel A. Leme Zelia I. P. Lobato Alice F. Alfieri Amauri A. Alfieri 《Veterinary research communications》2017,41(4):317-321
Seneca Valley virus (SVV) is the etiological agent of vesicular disease in pigs, clinically indistinguishable of classical viral vesicular infections, including foot-and-mouth disease. The first outbreaks of SVV infection in Brazil were reported in 2014. However, it was not known whether the virus was circulating in Brazilian pig herds before this year. This study is a retrospective serological investigation of porcine health status to SVV in Brazil. Serum samples (n = 594) were grouped in before (2007–2013, n = 347) and after (2014–2016, n = 247) SVV outbreaks in Brazil. Twenty-three pig herds were analyzed, of which 19 and 4 were sampled before and after the beginning of SVV outbreaks, respectively. Two herds sampled after 2014 presented animals with SVV-associated clinical manifestations, while the other two housed asymptomatic pigs. Anti-SVV antibodies were evaluated by virus neutralization test. The results demonstrated that pig herds of different Brazilian geographical regions and distinct pig categories were negative to anti-SVV antibodies in sera obtained before 2014. Antibodies to SVV were detected only in serum samples obtained after 2014, particularly in herds with the presence of pigs with SVV-clinical signs. These results present robust serological evidence that the SVV was not present in the major Brazilian pig producing regions prior to 2014. 相似文献
35.
36.
Alica Chroňáková Viviane Radl Miloslav Šimek Dana Elhottová Michael Schloter 《Soil biology & biochemistry》2009,41(6):1132-1138
Pasture soils used for cattle overwintering may represent significant sources of N2O emissions from soils. Therefore, the long-term effect of cattle overwintering on the abundance and activity of a denitrifying community was explored. The study was performed at a cattle overwintering area in South Bohemia (Czech Republic), where three sites differing in the degree of animal impact were selected: severely impacted (SI) and moderately impacted (MI), as well as a control site with no impact (NI). N2O flux measurement and soil sampling were performed in spring and fall of 2005. The activity was measured in terms of potential denitrification activity. Bacterial nirK, nirS and nosZ genes were used as functional markers of the denitrifying communities; abundance was analyzed using a real-time PCR assay. Surprisingly, in situ N2O emissions were the highest in spring at MI and significantly differed from those at SI and NI, while in autumn, rates of emissions generally decreased. In contrast potential denitrification rates were highest at SI, followed by MI, and the lowest at NI. An overall significant shift in N2O/N2 molar ratio was shown in cattle impacted sites. The highest abundance of all genes measured at both sampling times was found at site SI, whereas at site MI increased numbers were observed only in spring. Our results indicate a strong influence of cattle on the abundance as well as the activity of microbes involved in denitrification. 相似文献
37.
Viviane Dal-Souto Frescura Aline Augusti Boligon Felipe Manzoni Barbosa Jéssica Maronez De Souza Maíne Alessandra Lerner Haywood Dail Laughinghouse IV 《Journal of plant nutrition》2018,41(10):1293-1302
Rosmarinus officinalis L. (rosemary), is a medicinal and aromatic species used in the cosmetics and food processing industries. The present study aimed to evaluate the biomass production, content, yield and chemical composition of the essential oil of rosemary in cultivation out of soil with different concentrations of nutrient solution in both harvests. The cultivation of rosemary was conducted in a protected system off the ground, with concentrations of nutrient solutions of 1, 2, 3, 4 and 5 dS.m?1 for fertigation with collections of leaves 100 and 160 days after planting. Nutrient solution concentration influences phytomass production and the content and yield of essential oil of rosemary, though not its chemical composition. The collection time does not interfere in the content of essential oil; however, does interfere in the chemical composition; the production of phytomass and the yield of oil per plant are higher in collections at 160 days after planting. 相似文献
38.
39.
Jenniffer Hauschildt Dias Maria Amélia Pupin Gabriela Saloni Duarte Viviane Lopes Brair Cleber Jonas Carvalho de Paula Marco Antonio Paula de Sousa Ribrio Ivan Tavares Pereira Batista Joanna Maria Gonçalves Souza-Fabjan Maria Emília Franco Oliveira Jeferson Ferreira Fonseca 《Reproduction in domestic animals》2020,55(7):844-850
This study assessed the efficiency of cervical relaxation protocol using none, half or full dose (1.0 mg) of oestradiol benzoate in Dorper ewes subjected to non-surgical embryo recovery (NSER). Thirty-six pluriparous ewes received progestogen sponge (60 mg) for 9 days plus eCG administration (300 IU i.m.) 24 hr before sponge removal. Ewes were not mated and were randomly assigned to receive at 16 hr before NSER 37.5 µg d-cloprostenol i.m. and different doses of oestradiol benzoate: 0.0 mg (0EB group; n = 12); 0.5 mg (0.5EB group; n = 12) or 1.0 mg of oestradiol (1.0EB group, n = 12). All ewes received oxytocin (50 IU) i.v. 20 min before NSER, which was performed 8 days after sponge removal. Corpora lutea were counted by transrectal ultrasonography 24 hr before NSER. After procedure, the ewes were kept in natural breeding period to check their post-NSER fertility. NSER was performed in 91.7% (33/36) of the animals with overall fluid recovery efficiency over 97% (p > .05). The cervical transposing with Hegar dilator was longer (p < .05) in 0EB (4.2 ± 0.3 min) compared to 0.5EB (1.7 ± 0.3 min) and 1.0EB group (1.5 ± 0.3 min). The cervical transposing with mandrel/catheter was longer (p < .05) in 0EB (2.4 ± 0.5 min) than 1.0EB group (1.3 ± 0.5 min). Overall duration of uterine flushing was 25.4 min with structure recovery rate of 43.5%, with no difference among groups (p > .05). The post-NSER fertility was higher (p < .05) in 0.0EB (90%) than 0.5EB group (36.4%). In conclusion, NSER can be successfully performed in Dorper ewes by using a cervical relaxation protocol without oestradiol benzoate. 相似文献
40.
Brunetti Isabella Alves Pereira Pâmela Castro Oliveira Danilo Sagrillos Silva Viviane Aline Oliveira Reis Rui Manuel Américo-Pinheiro Juliana Heloisa Pinê da Cruz Claudinei 《Water, air, and soil pollution》2022,233(3):1-14
Water, Air, & Soil Pollution - Decentralized tap water systems are an important drinking water source worldwide. A good quality, high-pressure continuous water supply (CWS) is always the target... 相似文献