Lingual squamous cell carcinoma in a California sea lion (Zalophus californianus).     

Tsuneo Sato  Tomomi Higuchi  Hisashi Shibuya  Shigeo Ohba  Sadao Nogami  Wataru Shirai  Hideaki Watanabe  Shigeaki Honda 《Journal of zoo and wildlife medicine》2002,33(4):367-370

A 28-yr-old female California sea lion (Zalophus californianus) in a commercial aquarium developed an ulcerated lingual tumor and died. Necropsy revealed a moderately differentiated squamous cell carcinoma. Immunohistochemical staining and electron microscopic studies revealed that the tumor cells were strongly positive with anti-keratin-cytokeratin antibody and had abundant tonofibrils and desmosomes. The neoplasm had metastasized to a mandibular lymph node.  相似文献   

Comment on "Differential rescue of light- and food-entrainable circadian rhythms"     

Mistlberger RE  Yamazaki S  Pendergast JS  Landry GJ  Takumi T  Nakamura W 《Science (New York, N.Y.)》2008,322(5902):675; author reply 675

Fuller et al. (Reports, 23 May 2008, p. 1074) reported that the dorsomedial hypothalamus contains a Bmal1-based oscillator that can drive food-entrained circadian rhythms. We report that mice bearing a null mutation of Bmal1 exhibit normal food-anticipatory circadian rhythms. Lack of food anticipation in Bmal1-/- mice reported by Fuller et al. may reflect morbidity due to weight loss, thus raising questions about their conclusions.  相似文献   

Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan     

Wataru SATO  Eddy SUKMAWINATA  Ryoko UEMURA  Takuya KANDA  Kanichi KUSANO  Yoshinori KAMBAYASHI  Takashi SATO  Yuhiro ISHIKAWA  Ryohei TOYA  Masuo SUEYOSHI 《Journal of Equine Science》2020,31(4):85

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Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4     

Ueda W  Tohno M  Shimazu T  Fujie H  Aso H  Kawai Y  Numasaki M  Saito T  Kitazawa H 《Veterinary immunology and immunopathology》2011,143(1-2):148-154

Peptidoglycan recognition proteins (PGRPs) are innate immune molecules that are present in most invertebrates and vertebrates. Mammals have four PGRPs, PGLYRP1-4. In the present study, we cloned the cDNAs encoding porcine PGLYRP3 and 4 from the esophagus of adult swine. The length of the complete open reading frames of porcine PGLYRP3 and 4 are identical and contain 1125bp encoding 374 amino acid residues. The amino acid sequences of these two proteins were more similar to their human orthologs (78.9% [PGLYRP3] and 73.9% [PGLYRP4]) than to their mouse orthologs (71.3% [PGLYRP3] and 67.9% [PGLYRP4]). Expression analysis revealed that both PGLYRP3 and 4 were more strongly expressed in digestive tract, especially the esophagus, than in immune organs such as spleen or mesenteric lymph nodes in both newborn and adult swine. To analyze the subcellular distribution of porcine PGLYRP1-4, we constructed transfectant cell lines. Western blot and flow cytometric analyses revealed that porcine PGLYRP3 and 4 are not only secreted, but also expressed on the cell surface, unlike PGLYRP1 and 2. These results should help contribute to the understanding of PGLYRP3- and 4-mediated immune responses via their recognition of intestinal microorganisms in newborn and adult swine.  相似文献   

<Emphasis Type="Italic">Verticillium tricorpus</Emphasis> causing lettuce wilt in Japan differs genetically from California lettuce isolates     

Toshiyuki Usami  Takeshi Kanto  Patrik Inderbitzin  Mizuho Itoh  Gan Kisaki  Yoshiyuki Ebihara  Wataru Suda  Yoshimiki Amemiya  Krishna V. Subbarao 《Journal of General Plant Pathology》2011,77(1):17-23

In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California.  相似文献   

Large-scale molecular dynamics simulations of self-assembling systems     

Klein ML  Shinoda W 《Science (New York, N.Y.)》2008,321(5890):798-800

Relentless increases in the size and performance of multiprocessor computers, coupled with new algorithms and methods, have led to novel applications of simulations across chemistry. This Perspective focuses on the use of classical molecular dynamics and so-called coarse-grain models to explore phenomena involving self-assembly in complex fluids and biological systems.  相似文献   

Mapping the potential distribution of shorebirds in Japan: the importance of landscape‐level coastal geomorphology     

Hazuki Arakida  Hiromune Mitsuhashi  Mahito Kamada  Kazuo Koyama 《水产资源保护:海洋与淡水生态系统》2011,21(6):553-563

Correlation between skeletal muscle fiber type and responses of a taste sensing system in various beef samples     

Yusuke Komiya  Wataru Mizunoya  Kurumi Kajiwara  Issei Yokoyama  Hideki Ogasawara  Keizo Arihara 《Animal Science Journal》2020,91(1)

The difference of muscle fiber type composition affects several parameters related to meat quality; however, the relationship between muscle fiber types and meat taste is unclear. To elucidate this relationship, we determined the taste of various beef samples using a taste sensor (INSENT SA402B) and analyzed its correlation with different muscle fiber type composition. We used 22 kinds of beef samples and measured nine tastes, including the relative and change of membrane potential caused by adsorption (CPA) values, using six sensors (GL1, CT0, CA0, AAE, C00, and AE1). The taste sensor analysis indicated positive value outputs for the relative C00, AAE, and GL1 values as well as for the CPA value of AAE, which corresponded to bitterness, umami, sweetness, and richness, respectively. We found significant positive correlations of the myosin heavy chain 1 (MyHC1) composition with umami taste, and with richness. This result suggests that high levels of slow MyHC1 can induce strong umami taste and richness in beef. We expect that our results will contribute to the elucidation of the relationship between muscle fiber types and meat palatability.  相似文献   

Metabolic process of the C-sugars on the translocation pathways of cucumber plants     

Wataru Ohkawa  Yoshinori KanayamaNaoko Daibo  Takahiro SatoManabu Nishiyama  Koki Kanahama 《Scientia Horticulturae》2010

Studies on the metabolic process of photoassimilates and enhancement of sugar accumulation into fruit are important in fruit crop production. The metabolic process of the ¹⁴C-photoassimilates in cucumber plants was analyzed with respect to the vascular system. At 4 h after the start of ¹⁴CO₂ feeding, the ¹⁴C-photoassimilates synthesized in a selected leaf on the main shoot were translocated to the vascular bundles of the internode just below the ¹⁴CO₂-fed leaf. The radioactivity of ¹⁴C-stachyose was as high as that of ¹⁴C-sucrose in the vascular bundles of petiole and internode just below the ¹⁴CO₂-fed leaf as well as in the midrib, while the radioactivity of ¹⁴C-stachyose was lower than that of ¹⁴C-sucrose in the mesophyll. The ¹⁴C-photoassimilates appeared to have been translocated without any metabolic change in the translocation pathways between the petiole and internode just below the ¹⁴CO₂-fed leaf, because the ratio of ¹⁴C-stachyose radioactivity in the two parts was similar. At 8 h after the start of ¹⁴CO₂ feeding, the ¹⁴C-photoassimilates were translocated to the fruit. In the vascular bundles of the peduncle, the ratios of the radioactivity of ¹⁴C-stachyose and ¹⁴C-raffinose were lower, and the ratio of the radioactivity of ¹⁴C-sucrose was higher, than that at the petiole and internode just below the ¹⁴CO₂-fed leaf at 8 h after the start of ¹⁴CO₂ feeding. Therefore, it seemed that ¹⁴C-stachyose and ¹⁴C-raffinose were hydrolyzed to ¹⁴C-sucrose in the peduncle.  相似文献   

cDNA cloning and expression analysis of two heat shock protein genes,Hsp90 and Hsp60, from a sterile Ulva pertusa (Ulvales,Chlorophyta)     

Tominaga  Hiroshi  Coury  Daniel A.  Amano  Hideomi  Miki  Wataru  Kakinuma  Makoto 《Fisheries Science》2012,78(2):415-429

Molecular chaperones are synthesized and accumulated under a variety of unfavorable conditions in all organisms. Heat shock protein 90 (Hsp90) and Hsp60, which are classified into the major classes of molecular chaperones, play important roles in cellular stress responses. In this study, we characterized sterile Ulva pertusa Hsp90 (UpHsp90) and UpHsp60 genes which may be involved in tolerance to thermal and heavy metal stresses in this alga. The UpHsp90 cDNA consisting of 2,118 nucleotides encoded a polypeptide of 705 amino acids (AA). On the other hand, the UpHsp60 cDNA consisting of 1,722 nucleotides encoded a protein whose predicted length was 573 AA. The AA sequence alignment and phylogenetic analyses showed that the UpHsp90 and UpHsp60 proteins were more similar to cytoplasmic Hsp90s and mitochondrial Hsp60s, respectively, than to other types of the respective Hsps. Southern blot analysis indicated that the sterile U. pertusa genome had at least two cytoplasmic Hsp90-encoding genes and two mitochondrial Hsp60-encoding genes. The UpHsp90 and UpHsp60 mRNA levels were significantly affected by diurnal and temperature changes, and slightly affected by exposure to heavy metals. These results suggest that UpHsp90 and UpHsp60 genes play particularly important roles in adaptation to diurnal and temperature changes.  相似文献