青花菜根肿病苗期抗性鉴定技术的建立     

张小丽  柴阿丽  刘玉梅  方智远  李宝聚  李占省 《植物保护学报》2017,44(1):110-114

为建立快速、有效的青花菜根肿病苗期抗性鉴定技术,将芸薹根肿菌Plamodiophora brassicae Woron.人工接种于高感根肿病青花菜自交系90196,研究了接种菌液浓度、接种寄主苗龄、接种基质p H和接种方法等对人工接种鉴定效果的影响。结果表明,在一定范围内,根肿病发病率及病情指数随着接种菌液浓度的升高而增大,接种菌液浓度为3×108CFU/m L时,发病率及病情指数分别为97.22%和86.11,可以反映寄主真实的抗性水平;接种寄主苗龄为2~6叶期均能使植株发病,但2~3叶期发病效果最佳;接种基质偏酸性(p H 5~6)有利于根肿病的发生;使用伤根灌菌法进行鉴定,青花菜根肿病发病率和病情指数均最高,分别为100.00%和88.10,优于蘸根法和浸芽法。用已知抗性水平的12个自交系和8个杂交种进行验证,鉴定结果表明该苗期抗性鉴定技术可客观反映供试材料的实际抗性水平。  相似文献   

白菜型油菜PABP5基因启动子的克隆及表达特性分析   总被引：1，自引：0，他引：1  

石磊  董彩华  白泽涛  郭学兰  柴国华  黄军艳  刘胜毅 《分子植物育种》2009,7(2)

本研究中利用基因组步移法从白菜型油菜中克隆到Poly(A)结合蛋白基因PABP5起始密码子上游长588 bp的一段序列,经软件分析预测表明,该序列具有典型的启动子结构,并且具有多个与花药特异性表达相关的顺式作用元件,如TATA box,CAAT box,AGAAA motif,AGGTCA motif,AAACCCTAA motif,GTGA motif等.为了研究该片段的表达特性,本文将克隆到的序列置换pPBI121中的CaMV35S启动子,驱动其下游的GUS基因,构建了植物表达载体,转化拟南芥,获得了转基因植株.组织化学染色表明,在PABP5启动子Ppabp5的驱动下,报告基因GUS在拟南芥的根尖、第一片真叶叶原基以及花药中特异表达.本研究为PABP5基因的进一步功能研究奠定了基础.  相似文献   

浅析辽宁省清河区蔬菜施肥存在的问题及对策     

张宝兴  郭淑鲜  王亚平  柴春雁  尤雷 《北京农业》2010,(15)

简要分析了清河区蔬菜施肥概况,结合蔬菜生产实际,找出施肥上存在的问题,提出了蔬菜施肥要实行"控氮、稳磷、增钾、添微"及因作物施肥的对策。  相似文献   

浅析辽宁省喀左县生态农业存在问题及发展对策     

柴宝中 《北京农业》2010,(6)

辽宁省喀左县属典型的低山丘陵半干旱区,气候干旱,土壤瘠薄,农业生态环境恶化,经济基础薄弱,发展生态农业是该地区农业发展的必由之路。本文探讨了喀左地区农业生态环境存在的问题,并提出了相应的发展对策。  相似文献   

Synthesis and antifeedant activity of new oxadiazolyl 3(2H)-pyridazinones     

Cao S  Qian X  Song G  Chai B  Jiang Z 《Journal of agricultural and food chemistry》2003,51(1):152-155

A total of 20 new compounds containing the oxadiazolyl 3(2H)-pyridazinone moiety were synthesized. The structures of all the compounds were confirmed by (1)H NMR, IR, MS, and elemental analysis. Their insect antifeedant activities against Asiatic corn borer Ostrinia furnacalis (Guenee) were examined and compared with commercial azadirachtin. The compounds exhibited significant levels of activity. The feeding deterrency values of IIIa,j were 57% and 51% at 500 mg/kg concentration, respectively.  相似文献   

Spatial prediction of soil organic matter in the presence of different external trends with REML-EBLUP   总被引：3，自引：0，他引：3  

Xurong Chai  Chongyang Shen  Yuanfang Huang 《Geoderma》2008,148(2):159-166

The objective of our study was to compare the performance of the empirical best linear unbiased predictor (E-BLUP) with residual maximum likelihood (REML) with that of regression kriging (RK) for predicting soil organic matter (SOM) with the presence of different external drifts. The study was conducted on a 933 km² area in Pinggu district of Beijing. Terrain attributes (elevation, slope and topographic wetness index) calculated from DEM were used as external drift variable. The root mean squared errors (RMSE) and the mean squared deviation ratio (MSDR) were used to assess the accuracy of prediction and the goodness of fit of the theoretical estimate of error respectively. RK resulted in both the most and least accurate predictions. REML-EBLUP provided more correct residual variogram models than RK for each trend model. Our results have shown that when the value of adjusted R² is greater than 0.45, there is litter difference in the ability to increase the accuracy between REML-EBLUP and RK; and when the value is less than 0.45, the performance of REML-EBLUP is significantly better than RK. It also suggested that topographical data can further improve the accuracy of the spatial predictions of SOM by using RK and REML-EBLUP.  相似文献   

Modelling climate-related variability of tuna populations from a coupled ocean–biogeochemical-populations dynamics model     

Patrick Lehodey  Fei Chai  John Hampton 《Fisheries Oceanography》2003,12(4-5):483-494

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Frequency distribution of Echinococcus granulosus in dog populations in the Xinjiang Uygur Autonomous Region, China.     

R Ming  H D Tolley  F L Andersen  J Chai  Q Chang 《Veterinary parasitology》1992,43(3-4):233-241

Age-prevalence and age-intensity data of Echinococcus granulosus in dog populations were collected in four counties in the Xinjiang Uygur Autonomous Region, People's Republic of China. The frequency distribution of the parasite per dog was adequately described by the negative binomial model, with parameter k being 0.0571. The mathematical model of the life cycle of E. granulosus constructed by Roberts et al. (1986--Parasitology, Vol. 92, pages 621-641) was used to estimate the epidemiologic parameters of E. granulosus in Xinjiang. This model showed that the prevailing infection pressure on dogs was 0.4560 infectious insults year-1 and the mean length of an infection was 1.4975 years. The effect of acquired immunity on the prevalence of E. granulosus in dogs was considered to be insignificant.  相似文献   

庐山上的景天酸代谢植物     

蔡竹平 《江西农业大学学报》1992,14(3):242-245

本文讨论庐山的景天酸代谢植物的分布、表现特征、代谢途径和生理生态。根据Kluge和Ting判断CAM植物的四条标准,庐山上的CAM植物分布于20多个科,其中有些种类具有重要的经济价值。根据植物对CO_2的固定途径不同,可把庐山上的CAM植物分为两种类型,一种是在个体发育所有阶段中始终保持CAM途径;另一种是随植物生长发育情况和环境条件的变化而改变CO_2固定途径。本文指出庐山上的CAM植物具有对干旱环境的适应性。  相似文献   

20种植物提取物对马铃薯晚疫病菌的抗菌活性研究     

曹静  客绍英  王树桐  柴凤瑞  李川 《勤云标准版测试》2005,(12)

在室内测试了20种植物提取物对马铃薯晚疫病菌的抑菌作用,结果表明9种植物提取物对 孢子囊萌发具有显著的抑制作用,6种植物提取物对孢子囊产生具有显著的抑制作用。其中Ts-86、 Ts-39、Ts-109和Ts-121四种提取物抑菌效果与化学药剂“Ridomil”3000倍达到同一显著水平,并对 其进行了进一步测试。结果表明,Ts-86对马铃薯晚疫病菌产孢及孢子囊萌发的抑制效果显著优于另 外3种提取物,与“Ridomil”3000倍达到同一显著水平。  相似文献