Apparent ELISA detection times for albuterol after administration with the torpex equine inhaler device     

Dirikolu L  Mollett BA  Troppmann A  Woods WE  Bratton C  Cashman CP  Schroedter D  Mayer B  Lehner AF  Karpiesiuk W  Hughes C  Boyles J  Harkins JD  Tobin T 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(3):297-307

Single doses of one, three, and six actuations (120 micro g albuterol/actuation) and multiple daily doses (six actuations per dose four times daily) for 5 days of aerosol albuterol sulfate were sequentially administered to each of six horses using an equine inhaler device (Torpex, 3M Animal Care Products, St. Paul, MN [corrected] and Boehringer Ingleheim Vetmedica, Inc., St. Joseph, MO [corrected]). A 2-week washout period was allowed between each dose. ELISA testing revealed no evidence of albuterol in urine at 24 hours after any single-dose administration. Results indicated that 48 hours or longer should be allowed for albuterol to be cleared from urine after single doses. When given at the maximum recommended rate of six actuations per dose four times a day for 5 days, urine samples tested by ELISA showed no evidence of albuterol at 48 hours after the final dose. Testing of nasal swabs by ELISA demonstrated the presence of albuterol for 8 hours after each single dose, and some horses might have detectable levels of albuterol in nasal swabs for several days following administration of multiple doses. As a guideline for withdrawal time, 72 hours or longer should be allowed after administration of aerosol albuterol sulfate to horses before participation in equestrian competitions that are regulated for detection of certain performance-enhancing substances. However, these recommendations were based on a small sample of horses and the specific ELISA test used and interpreted as described. Factors specific to individual horses may influence these detection times.  相似文献   

Dispersal and mortality of translocated thick‐shelled river mussel Unio crassus Philipsson, 1788 adults revealed by radio tracking     

Katarzyna Zaj&#x;c  Tadeusz A. Zaj&#x;c  Pawe&#x; Adamski  Wojciech Biela&#x;ski  Adam M. &#x;miel  Anna M. Lipi&#x;ska 《水产资源保护:海洋与淡水生态系统》2019,29(3):331-340

Genotype analysis of Campylobacter jejuni isolated from broilers in Poland     

Wojciech L  Bednarski M  Wieliczko A  Ugorski M 《Polish journal of veterinary sciences》2005,8(1):41-47

Twenty-six Campylobacter jejuni strains isolated from poultry were analyzed by genotypic typing including ITS-profiling, REP- and ERIC-PCR. ITS-profiling revealed the presence of 8 different genotypes. Amplification of REP sequences by PCR gave similar results with 10 different genotypes. ERIC-PCR was found to be the most discriminatory for typing C. jejuni. As many as 13 different DNA patterns were obtained with this technique. Based on data obtained it was found that C. jejuni isolates recovered from broilers at the slaughterhouses in southwest Poland are characterized by a high degree of genetic heterogeneity.  相似文献   

Genomics. Not junk after all     

Makalowski W 《Science (New York, N.Y.)》2003,300(5623):1246-1247

  相似文献   

A manually controlled TDR soil moisture meter operating with 300 ps rise-time needle pulse     

Marek A. Malicki  Wojciech M. Skierucha 《Irrigation Science》1989,10(2):153-163

Summary The principle of operation of a simple, manually controlled Time Domain Reflectometer (TDR meter) for soil moisture measurements, which operates with a needle pulse of 300 ps rise-time, is described. A block diagram and constructions are also given. Construction of a switchless multiple sensor probe, having an inherent delay reference, is presented. Results of measurements of the soil dielectric constant as related to water content, for soils having different bulk densities, textures and humus content show a high correlation. The results agree closely with other investigators measurements with different, more expensive, TDR instruments. The general principle of microprocessor-controlled TDR operated soil moisture meter is considered.Patent pending  相似文献   

Fertile male tortoiseshell cat with true chimerism 38,XY/38,XY     

Monika Bugno-Poniewierska  Barbara Kij  Wojciech Witarski  Magdalena Wojtaszek  Anna Radko  Angelika Podbielska  Izabela Szczerbal  William J. Murphy 《Reproduction in domestic animals》2020,55(9):1139-1144

The tortoiseshell coat colour is characteristic to female cats, and its occurrence in tomcats is very rare and associated with chromosome abnormalities (additional copy of X chromosome). The aim of this study was identification of the genetic basis of a case of tortoiseshell colour in a fertile Maine coon tomcat. Cytogenetic and molecular genetic studies were carried out with painting molecular probes (WCPP) specific to the X and Y sex chromosomes as well as a DNA microsatellite panel for the parentage verification of cats. Cytogenetic analysis revealed only a single set of sex chromosomes typical for male – 38,XY. The results of the microsatellite polymorphism obtained from DNA showed three alleles in locus FCA201 and four alleles in loci FCA149 and FCA441 in different tissues (blood, hair roots and testicles). Based on these results, the case was diagnosed as a true chimerism 38,XY/38,XY. To the best of our knowledge, this is the first case of a 38,XY/38,XY chimera diagnosed in cats, confirmed by genetic analysis.  相似文献   

Survivability and developmental competences of domestic cat (Felis catus) oocytes after Cryotech method vitrification     

Agnieszka Nowak  Joanna Kochan  Ewa Świętek  Barbara Kij  Sylwia Prochowska  Wojciech Witarski  Monika Bugno-Poniewierska  Wojciech Niżański 《Reproduction in domestic animals》2020,55(8):992-997

The aim of this study was to evaluate the applicability of the Cryotech technique for the vitrification of domestic cat (Felis catus) oocytes, as a model for other feline species threatened with extinction. This technique, in which oocytes are stored in a minimal volume of medium, is already widely used in human assisted reproductive technology. In the first part of this study, a viability test (EtBr/FDA) was used to evaluate the toxicity of the vitrification media (solutions). After IVM, oocytes were placed in vitrification and warming solutions according to the manufacturer's procedure, with or without exposure to liquid nitrogen. The solutions and the vitrification procedure each caused a reduction in oocyte viability, with survival rates of 71.4% in oocytes exposed to the Cryotech media (without cooling in liquid nitrogen), and 62% in oocytes that were vitrified. In the second part of the experiment, parthenogenetic activation was used to evaluate the developmental potential of oocytes previously vitrified using the Cryotech method. After warming, the oocytes were activated using a combination of 0.7 µM ionomycin in TCM 199 medium (5 min) followed by 2 mM 6-DMAP in TCM 199 supplemented with 10% FBS (3 hr), then cultured and evaluated every 24 hr for parthenogenetic cleavage. In the experimental group, 23/50 (46%) cleaved embryos were obtained. Domestic cat oocytes, vitrified by the Cryotech method, are characterized by high survival rates. However, it is necessary to improve the technique to increase the developmental competence of embryos obtained from vitrified oocytes.  相似文献   

Analysis of genotype-by-environment interaction in a multisite progeny test with Scots pine for supporting selection decisions     

Chmura  Daniel J.  Barzdajn  Władysław  Kowalkowski  Wojciech  Guzicka  Marzenna  Rożkowski  Roman 《European Journal of Forest Research》2021,140(6):1457-1467

European Journal of Forest Research - In multisite forest genetic experiments, the presence of genotype?×?environment interaction (GEI) is common. GEI may negatively affect the...  相似文献   

Molecular characteristics of Polish field strains of Marek's disease herpesvirus isolated from vaccinated chickens     

Grzegorz Wo?niakowski  El?bieta Samorek-Salamonowicz  Wojciech Kozdruń 《Acta veterinaria Scandinavica》2011,53(1):10

Background

Twenty-nine Marek''s disease virus (MDV) strains were isolated during a 3 year period (2007-2010) from vaccinated and infected chicken flocks in Poland. These strains had caused severe clinical symptoms and lesions. In spite of proper vaccination with mono- or bivalent vaccines against Marek''s disease (MD), the chickens developed symptoms of MD with paralysis.Because of this we decided to investigate possible changes and mutations in the field strains that could potentially increase their virulence. We supposed that such mutations may have been caused by recombination with retroviruses of poultry - especially reticuloendotheliosis virus (REV).

Methods

In order to detect the possible reasons of recent changes in virulence of MDV strains, polymerase chain reaction (PCR) analyses for meq oncogene and for long-terminal repeat (LTR) region of REV were conducted. The obtained PCR products were sequenced and compared with other MDV and REV strains isolated worldwide and accessible in the GeneBank database.

Results

Sequencing of the meq oncogene showed a 68 basepair insertion and frame shift within 12 of 24 field strains. Interestingly, the analyses also showed 0.78, 0.8, 0.82, 1.6 kb and other random LTR-REV insertions into the MDV genome in 28 of 29 of strains. These genetic inserts were present after passage in chicken embryo kidney cells suggesting LTR integration into a non-functional region of the MDV genome.

Conclusion

The results indicate the presence of a recombination between MDV and REV under field conditions in Polish chicken farms. The genetic changes within the MDV genome may influence the virus replication and its features in vivo. However, there is no evidence that meq alteration and REV insertions are related to the strains'' virulence.  相似文献   

Influence of Catchment Characteristics and Flood Type on Relationship Between Streamwater Chemistry and Streamflow: Case Study from Carpathian Foothills in Poland     

Joanna P. Siwek  Miros?aw ?elazny  Wojciech Che?micki 《Water, air, and soil pollution》2011,214(1-4):547-563

The study aimed to determine the influence of catchment characteristics and flood type on the relationship between streamflow and a number of chemical characteristics of streamwater. These were specific electrical conductivity (SC), pH, the concentrations of main ions (Ca²⁺, Mg²⁺, Na⁺, K⁺, HCO ₃ ^? , SO ₄ ^2? , and Cl^?), and nutrients (NH ₄ ⁺ , NO ₂ ^? , NO ₃ ^? , and PO ₄ ^3? ). These relationships were studied in three small catchments with different geological structure and land use. Several flood types were distinguished based on the factors that initiate flooding and specific conditions during events. Geological factors led to a lower SC and main ion concentrations at a given specific runoff in catchments built of resistant sandstone versus those built of less resistant sediments. A lower concentration of nutrients was detected in the semi-natural woodland catchment versus agricultural and mixed-use catchments, which are strongly impacted by human activity. The strongest correlation between streamflow and the chemical characteristics of water was found in the woodland catchment. Different types of floods were characterized by different ion concentrations. In the woodland catchment, higher SC and higher concentrations of most main ions were noted during storm-induced floods than during floods induced by prolonged rainfall. The opposite was true for the agricultural and mixed-use catchments. During snowmelt floods, SC, NO ₃ ^? , and most main ion concentrations were higher when the soil was unfrozen in the agricultural and mixed-use catchments versus when the soil was frozen. In the case of the remaining nutrients, lower concentrations of NH ₄ ⁺ were detected during rain-induced floods than during snowmelt floods. The opposite was true of PO ₄ ^3? .  相似文献