Antiproliferative activity of melanoidins isolated from heated potato fiber (potex) in glioma cell culture model     

Langner E  Nunes FM  Pozarowski P  Kandefer-Szerszeń M  Pierzynowski SG  Rzeski W 《Journal of agricultural and food chemistry》2011,59(6):2708-2716

Potex constitutes a potato fiber preparation widely used as an ingredient to meat and bakery products which thermal treatment results in creation of new compounds. Melanoidins are high molecular weight brown end products of Maillard reaction, and few data presenting tumor cell growth inhibiting activity of melanoidins have been reported. Thus, in present study we utilized water extract of Potex roasted (180 °C for 2 h), whose chemical characterization revealed the presence of melanoidin complexes. Heated Potex extract inhibited C6 glioma cell proliferation in a dose-dependent manner measured by MTT method. High molecular weight components present in initial extract were responsible for stronger antiproliferative effect compared with low molecular weight fraction. Impaired MAPK (mitogen-activated protein kinase) and Akt signaling was found in cells treated with the extract. Moreover, flow cytometry analyses revealed the extract to induce G1/S arrest in glioma cells. Simultaneously, Western blot analysis showed elevated levels of p21 protein with concomitant decrease of cyclin D1. In conclusion, observed antiproliferative activity of melanoidins present in heated Potex was linked to disregulated MAPK and Akt signaling pathways, as well as to cell cycle cessation. These results suggest potential application of Potex preparation as a functional food ingredient and chemopreventive agent.  相似文献   

Riboflavin content in the coelomocytes of contrasting earthworm species is differentially affected by edaphic variables including organic matter and metal content     

Barbara Plytycz  Malgorzata Klimek  Beata Anna Klimek  Wojciech Szymanski  Jerzy Kruk  A. John Morgan 《Pedobiologia》2011

The riboflavin content in extruded coelomocyte lysates derived from Dendrodrilus rubidus may serve as a sensitive bioindicator of soil metal pollution: the vitamin (B2) content has previously been found to be high in worms from unpolluted soil but low in worms inhabiting Zn/Pb mine soils, aerially deposited Ni-contaminated soil, and in worms experimentally transferred from clean soil to the metalliferous field soils. The aim of the present work was to extend these observations by comparing the number and riboflavin composition of coelomocytes retrieved from three lumbricid species (Allolobophora chlorotica, Dendrobaena veneta, Eisenia andrei) after 4-week exposures to an unpolluted commercial soil, two geochemically contrasting unpolluted field soils, and two different Zn/Pb/Cd-polluted soils from the Bukowno district in South Poland. Whilst eco-physiologically contrasting, these three earthworm species share the trait of possessing relatively high numbers of eleocytes, a category of immune-competent coelomocyte rich in autofluorescent riboflavin. Spectroflurometric analysis indicated that coelomocyte riboflavin content in worms maintained in strongly metalliferous soils or in unpolluted sandy-clay and loamy-sand soils was increased in coleomocytes from epigeic D. veneta and E. andrei species, whilst was decreased in endogeic A. chlorotica. In conclusion, the riboflavin content of earthworm coelomocytes is affected in species-specific ways by edaphic variables, including organic matter and metal pollution.  相似文献   

Rapid detection of Marek's disease virus in feather follicles by loop-mediated amplification     

Woźniakowski G  Samorek-Salamonowicz E  Kozdruń W 《Avian diseases》2011,55(3):462-467

Marek's disease (MD) remains a serious problem in the production of poultry. The disease is caused by Marek's disease virus (MDV), and despite the ubiquitous use of vaccination to control losses, MD still affects poultry farming worldwide. The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the simple and inexpensive detection of MDV in feather tips of chickens. Two pairs of specific primers complementary to the meq oncogene of MDV were designed, targeting the sequence of the very virulent MDV strain, RB1B. Bst polymerase was used for the isothermal amplification of viral DNA at 65 C for 90 min in a water bath. The fluorescence signal was identified in MDV-positive samples after the addition of SYBR Green and ultraviolet (UV) illumination. The sensitivity of LAMP was 2 log 10 plaque-forming units (PFU)/ml of HPRS16 and 10(3) copies/il of plasmid containing the target gene (meq) and was equal in sensitivity to PCR amplification. Due to the use of three sets of primers, LAMP was highly specific for MDV-1 DNA. The developed LAMP technique is a rapid and simple tool for the specific detection of MDV in samples of feathers taken from live chickens. Since the use of thermocyclers is not necessary for LAMP assay, it can be conducted by small laboratories and even field veterinarians.  相似文献   

Erratum to: Coarse woody debris of Fagus sylvatica produced a quantitative organic carbon imprint in an andic soil     

Viliam Pichler  Erika Gömöryová  Marián Homolák  Magdaléna Pichlerová  Wojciech Skierucha 《Journal of Forest Research》2015,20(3):363-363

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Genetic comparison of <Emphasis Type="Italic">Ips duplicatus</Emphasis> (Sahlberg, 1836) (Coleoptera: Curculionidae,Scolytinae) populations from Europe and Asia     

Ferenc Lakatos  Wojciech Grodzki  Qing-He Zhang  Christian Stauffer 《Journal of Forest Research》2007,12(5):345-349

The distribution of the double spined spruce engraver beetle Ips duplicatus ranges from Scandinavia and northeastern Europe to northern Asia. In Europe, I. duplicatus usually is associated with I. typographus on Picea abies, and due to morphological similarities and similar gallery constructions the damage and significance of I. duplicatus are often not recognised and thus underestimated. I. duplicatus has been recently reported from the southern part of Poland, the Czech Republic, Slovakia, northern parts of Austria and Germany; records are missing from many other central European countries (e.g., Hungary). The species became an important pest in some parts of central Europe, and continuous outbreaks of this bark beetle have been reported in Inner Mongolia, China, since the 1950s. The aim of this study was to compare I. duplicatus populations from Europe and Asia by genetic means using the analysis of the mitochondrial DNA. Individuals of I. duplicatus populations from China, Poland, the Czech Republic and Slovakia were collected, and 520 bp fragments of the cytochrome oxidase I (COI) gene were analysed. Four haplotypes were detected and a sequence divergence of 0.8% was found between the populations from China and Europe. These differences associate with behavioural differences in the pheromone bouquet and behavioural response of the two groups. Within Europe three haplotypes were found, but due to the small sample size no significant geographical distribution was demonstrated.  相似文献   

Lupinus anatolicus — a new lupin species of the old world     

Wiktor Świ  cecicki  Wojciech K. Świ  cecicki  Bogdan Wolko 《Genetic Resources and Crop Evolution》1996,43(2):109-117

Summary During a collecting mission in South-West Turkey some lupin plants differing from Lupinus pilosus Murr., L. micranthus Guss. and L. angustifolius L., wild-growing in this region were found. These plants markedly distinguished from a dwarf habit of L. micranthus and exuberant L. pilosus. As found later, these plants with regard to many traits differed still more from the remaining lupin species of the Old World. The collected seeds of these plants were multiplied at the Plant Breeding Station in Wiatrowo. They were characterized by a smooth seed coat and according to classification of Gladstones could be referred to a group of European lupin crops containing the primitive species L. micranthus.New accession was compared to L. micranthus and L. pilosus considering 20 morphological, some physiological features and chemotaxonomic analyses. New accession was also artificially crossed to L. micranthus and L. pilosus but hybrid seeds were not obtained. The most pronounced morphological differences were in the height and exuberance of plants, the size of inflorescences and flowers, the size and coloration of strongly pubescent, ripen pods but first of all, the size and shape of seeds and seed coat surface.Differences in the protein and fat content in seeds as well as those in quantitative and qualitative composition of alkaloids also appear to be significant.Marked differences occurred also in the electrophoretic phenotype of isozymes. They consisted not only in differences of electrophoretic mobility of bands, but also in different number of bands.The obtained results enabled us to give the population of these plants a separate species name — Lupinus anatolicus.  相似文献   

Does Freezing Affect Sediment Sampling Results?     

Dabrowski  Wojciech  Mackie  R. Ian  Zielina  Michal 《Water, air, and soil pollution》2002,140(1-4):367-370

Sewer sediment sampling is subject to errors, and these canoften make a significant contribution to the discrepanciesbetween predictions from mathematical models of pollutiontransport and what actually happens. Deep freezing ofsediment and cutting samples is the most reliable method ofsampling, unfortunately it is expensive and is limited mostlyto trunk sewers of diameters exceeding 800 mm. The impact offreezing sediment collected from filter backwash watersedimentation tank on the size distribution of particles below180 μm was investigated, demonstrating that the process had asignificant impact. Small particles are known to contribute toheavy metal content in sewer sediments, so they are the subjectof special concern.  相似文献   

Heavy metals in the slope deposits of loess areas of the Lublin Upland (E Poland)     

Wojciech Zgłobicki  Jan Rodzik 《CATENA》2007

The concentration of heavy metals (Cd, Cu, Pb, Zn) has been analyzed in Holocene slope deposits of the loess agricultural area in the Eastern Poland. Five profiles were examined. In general, low concentrations of the examined metals were determined, on average 50% of the samples exceeded the designated regional, geochemical background. The average concentrations were: cadmium < 0.5 ppm, copper up to 15, and lead up to 20 and zinc up to 70 ppm, showing a vertical diversity in the profile. Higher heavy metal concentrations in Holocene deposits are related to soil formation or accumulation of organic matter. The direct influence of human factors was most visible in recent deposits. It includes the modification of the development conditions of soil-forming processes as well as the influence on the character of the colluvial deposits. Taking into account the ¹⁴C datings as well as the archeological and historical data the age and rate of sedimentation of deposits have been calculated. The average rate of the colluvia deposition varied from 1.3 to 7.3 mm/year depending on their age and the character of sedimentation.  相似文献   

New therapeutic approaches for equine protozoal myeloencephalitis: pharmacokinetics of diclazuril sodium salts in horses     

Dirikolu L  Karpiesiuk W  Lehner AF  Hughes C  Woods WE  Harkins JD  Boyles J  Atkinson A  Granstrom DE  Tobin T 《Veterinary therapeutics : research in applied veterinary medicine》2006,7(1):52-63, 72

Diclazuril is a triazine-based antiprotozoal agent which may have clinical application in the treatment of equine protozoal myeloencephalomyelitis (EPM). In this study, the use of the sodium salt diclazuril to increase the apparent bioavailability of diclazuril for the treatment and prophylaxis of EPM and various other Apicomplexan mediated diseases is described. In this study, diclazuril sodium salt was synthesized and administered to horses as diclazuril sodium salt formulations. The absorption, distribution, and clearance of diclazuril sodium salt in the horse are described. Diclazuril was rapidly absorbed, with peak plasma concentrations occurring at 8-24 hours following an oral mucosal administration of diclazuril sodium salt. The mean oral bioavailability of diclazuril as Clinacox was 9.5% relative to oral mucosal administration of diclazuril sodium salt. Additionally, diclazuril in DMSO administered orally was 50% less bioavailable than diclazuril sodium salt following an oral mucosal administration. It was also shown that diclazuril sodium salt has the potential to be used as a feed additive for the treatment and prophylaxis of EPM and various other Apicomplexan mediated diseases.  相似文献   

Temperature dependence of time domain reflectometry–measured soil dielectric permittivity     

Wojciech Skierucha 《植物养料与土壤学杂志》2009,172(2):186-193

The purpose of this study is to determine the temperature influence on the soil bulk dielectric permittivity, ?_b, calculated from the measurement of the electromagnetic‐wave velocity of propagation along the parallel waveguide in a TDR probe, i.e., a probe working in time domain reflectometry technique. The experimental evidence shows that the existing models do not completely describe the temperature effect. However, it has been confirmed that the observed temperature effect is the result of two competing phenomena: ?_b increases with temperature following the release of bound water from soil solid particles, and ?_b decreases with temperature increase following the temperature effect of free water molecules. It has been found that there is a soil type–characteristic moisture value, θ_eq, named the equilibrium water content, at which both competing phenomena compensate each other. The equilibrium water content, θ_eq, is correlated with the soil specific surface area. Based on knowledge of θ_eq, a temperature‐correction formula is presented that adjusts the TDR soil‐moisture measurements at various temperatures to the corresponding value at 25°C. This decreases the absolute measurement error of soil moisture, θ_TDR, by the factor of 2 as compared to the uncorrected values.  相似文献