Survival change of ventral mesencephalon-derived progenitor cells after grafting into unilateral intact adult rat striatum     

Park MS  Lee HY  Li SP  Koh PO  Bahk JY  Chung BC  Kim MO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):25-30

Neural transplantation is one of the most promising treatments for neurodegenerative disorders. Survival rates of embryonic dopamine (DA) neurons following transplantation are low, between 2% and 20% in a number of animal models. To further establish survival changes of the transplanted gestational day 13.5 ventral mesencephalic (VM) cells into left intact adult rat striata so that design strategies of increasing survival of DA neurons, the tyrosine hydroxylase (TH) expression of VM-derived progenitor cells has been examined using immunohistochemistry and Western blot analysis. TH immunostaining revealed that the grafted VM cells developed to mature TH-positive neurons strongly at 3 weeks, peaked at 4 weeks, thereafter, gradually dropped following the degenerative expression of the grafted cells at both 5 and 6 weeks after transplantation. Western blot analysis also showed that the TH proteins were maximally expressed at 4 weeks post-grafting. Our finding suggested that the peak of surviving VM-derived TH positive cells occurred approximately 4 weeks after transplantation.  相似文献   

Increased collagen III in culled chicken meat after feeding dietary wood charcoal and vinegar contributes to palatability and tenderness     

Kohsyo Yamauchi  Noboru Manabe  Yoshiki Matsumoto  Shin‐ichi Takenoyama  Koh‐en Yamauchi 《Animal Science Journal》2014,85(4):468-480

We comprehensively evaluated meat quality in chickens fed a diet consisting of wood charcoal and vinegar (WCV) using food scientific and histological approaches. In culled hens, lipid and fatty acid in Musculus semimembranosus, cooking loss and sensory tests of whole thigh meat, and meat texture of breast meat were observed. In male broilers, cross section of M. semimembranosus was used for observations on muscle area, perimysium, non‐collagen total protein and total collagen content, and anti‐collagen I and III reactions. In frozen male broilers, conventional morphology of M. semimembranosus as well as chicken anti‐collagen III reaction to selected muscles of thigh meat and breast meat were compared between the control and WCV‐fed birds. Increased lipid and fatty acids, decreased cooking loss, high score in total evaluation for sensory test of thigh meat, and decreased meat texture values were observed for culled hens fed WCV. The higher values of muscle area, total collagen and collagen III were observed for broilers fed WCV. No perimysium collapse for M. semitendinosus or increased collagen III reactions of M. tensor fasciae latae, the flexor muscle group and M. pectoralis superficialis were observed for frozen muscles in the WCV group. These total results suggest that WCV produces palatable and tender meat by increasing collagen III.  相似文献   

Phenolic extractives in the trunk of Toxicodendron vernicifluum: chemical characteristics,contents and radial distribution     

Koh Hashida  Masanobu Tabata  Katsushi Kuroda  Yuichiro Otsuka  Satoshi Kubo  Rei Makino  Yoshitaka Kubojima  Mario Tonosaki  Seiji Ohara 《Journal of Wood Science》2014,60(2):160-168

Phenolic extractives in the trunk of Toxicodendron vernicifluum (syn. Rhus verniciflua) were investigated. Seventeen compounds, gallic acid, protocatechuic acid, (?)-fisetinidol-4β-ol, (?)-fisetinidol-4α-ol, 2-benzyl-2,6,3′,4′-tetrahydroxycoumaran-3-one, (?)-fustin, 1,2,3,6-Tetra-O-galloyl-β-d-glucose, (?)-epifustin, (+)-taxifolin, 1,2,3,4,6-penta-O-galloyl-β-d-glucose, (?)-garbanzol, (?)-fustin-3-O-gallate, (?)-epifustin-3-O-gallate, fisetin, sulfuretin, quercetin and butein, were identified from the heartwood extractives. It was found that only (+)-taxifolin which had 5,7-dihydroxy A-ring possessed a 3R configuration although other flavonoids which had 7-hydroxy A-ring possessed a 3S configuration. Quantitative analysis revealed that the total phenolic contents were much higher in the heartwood (5–7 wt%) than in the sapwood and bark (1–2 wt%), and (?)-fustin was the most abundant extractive in the heartwood (1.4–2.4 wt%). For the radial distribution of phenolic extractives, it was generally found that their content was lowest in the sapwood, increased to the highest in the outer heartwood, and then decreased in the inner heartwood.  相似文献   

Interlaboratory study of blood selenium determinations   总被引：1，自引：0，他引：1  

T S Koh 《Journal of the Association of Official Analytical Chemists》1987,70(4):664-667

Fifty-one laboratories from 14 countries participated in a survey on the determination of selenium (Se) in 8 bovine blood samples with Se concentrations ranging from 0.2 mumol/L (0.016 microgram/mL) to 14 mumol/L (1.1 micrograms/mL). The methods used (and the percentage of participants using each method) were fluorometry (61), hydride-generation atomic absorption spectrophotometry (AAS) (23), graphite-furnace AAS (6), gas chromatography (4), neutron activation analysis (4), and X-ray fluorometry (2). There was little difference in the mean Se results obtained by fluorometry or hydride-generation AAS (P greater than 0.05). Mean intralaboratory coefficients of variation (CVs) from known replicates ranged from 4 to 14% for all samples. Interlaboratory CVs were related to blood Se concentration and increased to 55% at Se levels below 0.4 mumol/L (0.032 microgram/mL). Laboratories that used quality control (QC) schemes had lower interlaboratory CVs than those that did not, but the advantage began to diminish at blood Se concentration below 0.4 mumol/L (0.032 microgram/mL). The high interlaboratory CVs, coupled with the false assurance from the low intralaboratory CVs and the ineffectiveness of the QC schemes at blood Se concentrations below 0.4 mumol/L (0.032 microgram/mL), are of concern in diagnosis of marginal Se deficiency in livestock where the concentrations of interest are in the range 0.15-0.5 mumol/L (0.012-0.039 microgram/mL).  相似文献   

Nonproteolytic neuroprotection by human recombinant tissue plasminogen activator     

Kim YH  Park JH  Hong SH  Koh JY 《Science (New York, N.Y.)》1999,284(5414):647-650

Human recombinant tissue plasminogen activator (tPA) may benefit ischemic stroke patients by dissolving clots. However, independent of thrombolysis, tPA may also have deleterious effects on neurons by promoting excitotoxicity. Zinc neurotoxicity has been shown to be an additional key mechanism in brain injuries. Hence, if tPA affects zinc neurotoxicity, this may provide additional insights into its effect on neuronal death. Independent of its proteolytic action, tPA markedly attenuated zinc-induced cell death in cortical culture, and, when injected into cerebrospinal fluid, also reduced kainate seizure-induced hippocampal neuronal death in adult rats.  相似文献   

Alkaline weight reduction monitoring of sea-island type polyamide microfiber fabrics     

Yoon-Jung Kwon  Joonseok Koh  Myung Joon Oh  Sung Dong Kim 《Fibers and Polymers》2006,7(1):20-25

The alkaline dissolution behavior of sea-island type polyamide microfibers were successfully monitored using a cationic dye staining method. Weight reduction behavior of the alkali-treated microfiber fabrics and the treated fabrics stained with cationic dye were investigated in a comparative manner. The termination of dissolution monitored by both methods was also confirmed by scanning electron microscopy.  相似文献   

Dye-resist properties of reactive dye-resist agents in acid dyeing of silk     

Sung Hee Park  Myung Joon Oh  Joonseok Koh 《Fibers and Polymers》2007,8(6):601-607

The dye-resist effect of reactive dye-resist agents in acid dyeing of silk was investigated. The dye-resist agent containing dichlorotriazine achieved a higher resist effectiveness than others since they make a charge barrier against diffusion in the silk fiber periphery due to the high reactivity of dichlorotriazine group. Especially, the increase in the number of ionic groups in acid dyes leads to better electrostatic repulsion of reactive dye resist agent treated silk and thus improves the resist effectiveness. However, the hydrophobicity effect of acid dyes on the dye-resist properties was relatively minor.  相似文献   

Dyeing and fastness properties of a reactive disperse dye on PET, nylon, silk and N/P fabrics     

Jin-Seok Bae  Jong Ho Park  Joonseok Koh  Sung Dong Kim 《Fibers and Polymers》2006,7(2):174-179

Dyeing and color fastness properties of a reactive disperse dye containing an acetoxyethylsulphone group on PET, Nylon, silk and N/P fabrics were examined. The reactive disperse dye exhibited almost the same dyeing properties on PET fabric as a conventional disperse dye except the level of dye uptake. The most appropriate pH and dyeing temperature for the dyeing of Nylon fabric were 7 and 100°C respectively. The build-up on Nylon fabric was good and various color fastnesses were good to excellent due to the formation of the covalent bond. Application of the reactive disperse dye on silk fabric at pH 9 and 80°C yielded optimum color strength. The rate of dyeing on Nylon fabric was faster than that on PET fabric when both fabrics were dyed simultaneously in a dye bath, accordingly color strength of the dyed Nylon was higher. The reactive disperse dye can be applied for one-step and one-bath dyeing of N/P mixture fabric with good color fastness.  相似文献   

Dyebath reuse in dyeing of nylon microfiber non-woven fabric with 1:2 metal complex dyes     

Joon Seok Koh  Yong Geol Kim  Jae Pil Kim 《Fibers and Polymers》2001,2(1):35-40

Dyebath used for metal complex dyeing of nylon microfiber was recycled to reduce the overall amounts of metal complex dyeing effluents. Instead of discharging the dyebath after each dyeing cycle, the residual dyebath was analyzed spectrophotometrically and reconstituted to the required concentration of dyes and auxiliaries. Dyebaths were reused eight times and the CIELAB coordinates of dyed samples were measured after each recycling. Color difference (ΔE*) between the sample dyed in the fresh bath and that from reused dyebath was maintained below 1.5. The levelness and fastness of dyed fabrics from recycled dyebath were not impaired either. Chromium content of each recycled dyebath was similar to that of the first residual dyebath.  相似文献