全文获取类型
收费全文 | 675篇 |
免费 | 48篇 |
国内免费 | 3篇 |
专业分类
林业 | 30篇 |
农学 | 13篇 |
基础科学 | 2篇 |
159篇 | |
综合类 | 156篇 |
农作物 | 25篇 |
水产渔业 | 37篇 |
畜牧兽医 | 266篇 |
园艺 | 6篇 |
植物保护 | 32篇 |
出版年
2023年 | 5篇 |
2021年 | 10篇 |
2020年 | 12篇 |
2019年 | 10篇 |
2018年 | 12篇 |
2017年 | 13篇 |
2016年 | 16篇 |
2015年 | 23篇 |
2014年 | 18篇 |
2013年 | 23篇 |
2012年 | 31篇 |
2011年 | 35篇 |
2010年 | 27篇 |
2009年 | 11篇 |
2008年 | 26篇 |
2007年 | 24篇 |
2006年 | 24篇 |
2005年 | 37篇 |
2004年 | 26篇 |
2003年 | 22篇 |
2002年 | 21篇 |
2001年 | 25篇 |
2000年 | 23篇 |
1999年 | 25篇 |
1998年 | 18篇 |
1997年 | 10篇 |
1996年 | 15篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 14篇 |
1992年 | 16篇 |
1991年 | 12篇 |
1990年 | 17篇 |
1989年 | 12篇 |
1988年 | 10篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 12篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1981年 | 5篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 3篇 |
排序方式: 共有726条查询结果,搜索用时 125 毫秒
41.
X-ray absorption spectroscopy was used to probe the chemical and structural environment of vanadium in coal. It was found that vanadium exists in at least two environments, in both of which it was coordinated to oxygens. There was no evidence of vanadium in nitrogen (porphyrin) or sulfide environments. It was also found that the vanadium environments in the raw coal did not survive unchanged in a liquefaction process. These findings have implications for coal cleaning processes and for trace element release into the liquefaction process stream. 相似文献
42.
Films of silicon dioxide (SiO2) were deposited at room temperature by means of catalyzed binary reaction sequence chemistry. The binary reaction SiCl4 + 2H2O --> SiO2 + 4HCl was separated into SiCl4 and H2O half-reactions, and the half-reactions were then performed in an ABAB ellipsis sequence and catalyzed with pyridine. The pyridine catalyst lowered the deposition temperature from >600 to 300 kelvin and reduced the reactant flux required for complete reactions from approximately 10(9) to approximately 10(4) Langmuirs. Growth rates of approximately 2.1 angstroms per AB reaction cycle were obtained at room temperature for reactant pressures of 15 millitorr and 60-second exposure times with 200 millitorr of pyridine. This catalytic technique may be general and should facilitate the chemical vapor deposition of other oxide and nitride materials. 相似文献
43.
Aflatoxicol, a carcinogenic metabolite of the foodborne carcinogen aflatoxin B1 previously known only as a bioreduction product in vitro, was identified as the major aflatoxin metabolite in the plasma of Sprague-Dawley rats, a susceptible species, that had been doses orally or intravenously with aflatoxin B1 labeled with carbon-14. Alfatoxicol, however, was not detected in the plasma of similarly dosed mice and monkeys, which are both resistant to aflatoxin B1-induced cardinogenesis. The formation of aflatoxicol both in vitro and in vivo may be an indicatory of species sensitivity to aflatoxin-induced carcinogenesis and may be useful in the prediction of human susceptibility. 相似文献
44.
Significantly improved values of the zonal gravity harmonic coefficients J(39) J(4), and J(6) of Jupiter have been obtained from a preliminary analysis of Pioneer 11 spacecraft Doppler data taken while the spacecraft was near Jupiter. The new results, which will have an important application as boundary conditions for theoretical models of Jupiter's interior. are consistent with a planet in hydrostatic equilibrium. 相似文献
45.
We up-scaled the APSIM simulation model of crop growth, water and nitrogen dynamics to interpret and respond to spatial and temporal variations in soil, season and crop performance and improve yield and decrease nitrate leaching. Grain yields, drainage below the maximum root depth and nitrate leaching are strongly governed by interaction of plant available soil water storage capacity (PAWC), seasonal rainfall and nitrogen supply in the water-limited Mediterranean-type environment of Western Australia (WA). APSIM simulates the interaction of these key system parameters and the robustness of its simulations has been rigorously tested with the results of several field experiments covering a range of soil types and seasonal conditions in WA. We used yield maps, soil and weather data for farms at two locations in WA to determine spatial and temporal patterns of grain yield, drainage below the maximum root depth and nitrate leaching under a range of weather, soil and nitrogen management scenarios. On one farm, we up-scaled APSIM simulations across the whole farm using local weather and fertiliser use data and the average PAWC values of soil type polygons. On a 70 ha field on another farm, we used a linear regression of apparent soil electrical conductivity (ECa) measured by EM38 against PAWC to transform an ECa map of the field into a high resolution (5 m grid) PAWC map. We then used regressions of simulated yields, drainage below the maximum root depth and nitrate leaching on PAWC to upscale the APSIM simulations for a range of weather and fertiliser management scenarios. This continuous mapping approach overcame the weakness of the soil polygons approach, which assumed uniformity in soil properties and processes within soil type polygons. It identified areas at greatest financial and environmental risks across the field, which required focused management and simulated their response to management interventions. Splitting nitrogen applications increased simulated wheat yields at all sites across the field and decreased nitrate leaching particularly where the water storage capacity of the soil was small. Low water storage capacity resulted in both low wheat yields and large leaching loss. Another management option to decrease leaching may be to grow perennial vegetation that uses more water and loses less by drainage.Paper from the 5th European Conference on Precision Agriculture (5ECPA), Uppsala, Sweden, 2005 相似文献
46.
Wong J Krisch M Farber DL Occelli F Schwartz AJ Chiang TC Wall M Boro C Xu R 《Science (New York, N.Y.)》2003,301(5636):1078-1080
We report an experimental determination of the phonon dispersion curves in a face-centered cubic (fcc) delta-plutonium-0.6 weight % gallium alloy. Several unusual features, including a large elastic anisotropy, a small-shear elastic modulus C', a Kohn-like anomaly in the T1[011] branch, and a pronounced softening of the [111] transverse modes, are found. These features can be related to the phase transitions of plutonium and to strong coupling between the lattice structure and the 5f valence instabilities. Our results also provide a critical test for theoretical treatments of highly correlated 5f electron systems as exemplified by recent dynamical mean field theory calculations for delta-plutonium. 相似文献
47.
Galibert F Finan TM Long SR Puhler A Abola P Ampe F Barloy-Hubler F Barnett MJ Becker A Boistard P Bothe G Boutry M Bowser L Buhrmester J Cadieu E Capela D Chain P Cowie A Davis RW Dreano S Federspiel NA Fisher RF Gloux S Godrie T Goffeau A Golding B Gouzy J Gurjal M Hernandez-Lucas I Hong A Huizar L Hyman RW Jones T Kahn D Kahn ML Kalman S Keating DH Kiss E Komp C Lelaure V Masuy D Palm C Peck MC Pohl TM Portetelle D Purnelle B Ramsperger U Surzycki R Thebault P Vandenbol M Vorholter FJ 《Science (New York, N.Y.)》2001,293(5530):668-672
The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogen (N2) to ammonium (NH4+). We present here the annotated DNA sequence of the alpha-proteobacterium Sinorhizobium meliloti, the symbiont of alfalfa. The tripartite 6.7-megabase (Mb) genome comprises a 3.65-Mb chromosome, and 1.35-Mb pSymA and 1.68-Mb pSymB megaplasmids. Genome sequence analysis indicates that all three elements contribute, in varying degrees, to symbiosis and reveals how this genome may have emerged during evolution. The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments. 相似文献
48.
Discovery and directed evolution of a glyphosate tolerance gene 总被引:2,自引:0,他引:2
Castle LA Siehl DL Gorton R Patten PA Chen YH Bertain S Cho HJ Duck N Wong J Liu D Lassner MW 《Science (New York, N.Y.)》2004,304(5674):1151-1154
The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM-1 min-1 to 8320 mM-1 min-1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops. 相似文献
49.
Zhang Z Gildersleeve J Yang YY Xu R Loo JA Uryu S Wong CH Schultz PG 《Science (New York, N.Y.)》2004,303(5656):371-373
Posttranslational modifications of proteins regulate many biological processes, including metabolism, signal transduction, and gene expression. The synthetic challenges associated with generating homogeneous populations of selectively modified proteins, however, have hindered detailed studies of the effects of these modifications on protein structure and function. Here, we report an approach to the cotranslational synthesis of selectively glycosylated proteins in which the modified amino acid is genetically encoded. We show that myoglobin containing beta-N-acetylglucosamine (GlcNAc)-serine at a defined position can be expressed in Escherichia coli in good yield and with high fidelity. The beta-GlcNAc moiety can be recognized by a saccharide-binding protein, or subsequently modified with a galactosyltransferase to build more complex carbohydrates. This approach should be generally applicable to other posttranslational modifications such as protein phosphorylation, acetylation, and methylation. 相似文献
50.
Enzymatic catalysts in organic synthesis 总被引:4,自引:0,他引:4
C H Wong 《Science (New York, N.Y.)》1989,244(4909):1145-1152
The synthetic value of enzymes is being increasingly recognized. With an understanding of enzyme-catalyzed reactions and the techniques now available for the low-cost production and rational alteration of enzymes and for the design of new enzymatic activities, biocatalytic synthesis should become one of the most valuable synthetic methods. The fundamental as well as the practical issues of this rapidly growing area are described and its impact on the technology pertaining to synthesis is considered. 相似文献