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91.
92.
后备公猪的科学选育及利用直接影响猪场制种效果和经济效益,同时也能体现规模化猪场的制种技术与水平。文章针对后备公猪选育的基本环节和操作要点,从如何选种选育、科学饲养管理、合理配种利用等方面进行系统阐述,为规模化猪场后备公猪生产提供技术指导和参考依据。  相似文献   
93.
甲壳素对连作平邑甜茶生长、光合及抗氧化酶的影响   总被引:2,自引:0,他引:2  
以苹果连作土盆栽的平邑甜茶幼苗为试材,探讨了0、0.5、1.0和2.5 g·kg-1不同施入量的甲壳素对其光合速率、活性氧含量及抗氧化酶活性的影响。结果表明,1.0 g·kg-1的甲壳素处理能显著促进幼苗株高、地径,干样质量和根冠比的增加,根冠比为对照的1.51倍;明显提高了幼苗叶片光合色素含量、净光合速率和蒸腾速率,其中光合速率为对照的1.30倍;同时提高了幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,分别为对照的1.10倍、1.85倍、1.77倍和1.43倍;减少了叶片丙二醛(MDA)、过氧化氢(H2O2)和超氧阴离子的积累,分别为对照的73%、62%和34%,降低了脯氨酸(Pro)和可溶性糖含量。当甲壳素施用量为2.5 g·kg-1时则显著抑制平邑甜茶幼苗生长,降低幼苗叶片光合速率和抗氧化酶活性,并使超氧阴离子和脯氨酸含量明显上升。因此,适宜用量的甲壳素能减轻苹果的连作障碍。  相似文献   
94.
ABSTRACT

Aims: To assess the change in body condition score (BCS) during the early and late dry periods and its association with postpartum diseases and milk yield in grazing dairy cows from central Argentina.

Methods: BCS assessments during the dry period, and cow health and milk production records up to 90 days in milk (DIM), were collated for cows from 28 farms at monthly visits between 2007 and 2008. Cows were categorised into four groups; those in Group 1 (n=7,067) maintained or gained BCS during the early and late dry periods; Group 2 (n=2,615) maintained or gained BCS during the early dry period and lost BCS during the late dry period; Group 3 (n=1,989) lost BCS during the early dry period and maintained or gained BCS during the late dry period; and Group 4 (n=5,144) lost BCS during the early and late dry periods.

Results: Cows in Group 1 had reduced odds of having retained fetal membranes (RFM), metritis, and clinical mastitis up to 90 DIM than cows in Group 2 (p<0.001), but the odds of disease were similar to cows in Group 3. The odds of having RFM or clinical mastitis tended to be lower in cows in Group 1 than cows in Group 4 (p=0.08). The odds of cows being culled or dying during the first 90 DIM were lower for cows in Group 1 than for those in Groups 2, 3, and 4 (p≤0.05). Mean accumulated milk yield up to 90 DIM was higher in cows in Group 1 than Group 2 and Group 4 (p<0.001), but was similar to that of cows in Group 3 (p=0.28).

Conclusions and clinical relevance: Cows that lost BCS during the late dry period had increased odds of being diagnosed with several postpartum diseases and had decreased milk yield compared to cows that maintained or gained BCS during the entire dry period. Loss of BCS during any stage of the dry period was also associated with increased incidence of culling or death during the first 90 DIM. These results should raise awareness among dairy cattle producers of the importance of properly managing cow body condition during the dry period, especially during the late dry period.  相似文献   
95.
96.
Aeolian-fluvial interplay erosion regions are subject to intense soil erosion and are of particular concern in loess areas of northwestern China.Understanding the composition,distribution,and transport processes of eroded sediments in these regions is of considerable scientific significance for controlling soil erosion.In this study,based on laboratory rainfall simulation experiments,we analyzed rainfall-induced erosion processes on sand-covered loess slopes(SS)with different sand cover patterns(including length and thickness)and uncovered loess slopes(LS)to investigate the influences of sand cover on erosion processes of loess slopes in case regions of aeolian-fluvial erosion.The grain-size curves of eroded sediments were fitted using the Weibull function.Compositions of eroded sediments under different sand cover patterns and rainfall intensities were analyzed to explore sediment transport modes of SS.The influences of sand cover amount and pattern on erosion processes of loess slopes were also discussed.The results show that sand cover on loess slopes influences the proportion of loess erosion and that the compositions of eroded sediments vary between SS and LS.Sand cover on loess slopes transforms silt erosion into sand erosion by reducing splash erosion and changing the rainfall-induced erosion processes.The percentage of eroded sand from SS in the early stage of runoff and sediment generation is always higher than that in the late stage.Sand cover on loess slopes aggravates loess erosion,not only by adding sand as additional eroded sediments but also by increasing the amount of eroded loess,compared with the loess slopes without sand cover.The influence of sand cover pattern on runoff yield and the amount of eroded sediments is larger than that of sand cover amount.Furthermore,given the same sand cover pattern,a thicker sand cover could increase sand erosion while a thinner sand cover could aggravate loess erosion.This difference explains the existence of intense erosion on slopes that are thinly covered with sand in regions where aeolian erosion and fluvial erosion interact.  相似文献   
97.
  【目的】  西北旱地春玉米种植区磷肥过量施用,磷肥利用效率偏低。本研究旨在探究春玉米磷肥减施的可行性以及实现磷素高效利用的优化施肥方式,以期为该地区春玉米磷肥高效利用提供参考。  【方法】  在西北典型雨养农业区设置3年的定位田间试验,共设5个处理:对照 (CK,不施磷肥)、农户模式 (FP,磷用量P2O5 120 kg/hm2,撒施)、减磷撒施 (RP,磷用量P2O5 70 kg/hm2,撒施)、减磷条施 (BF,磷用量P2O5 70 kg/hm2,条施)、硫酸铵模式 (SA,采用硫酸铵氮肥替代尿素氮肥,其他同减磷撒施处理)。在玉米四叶期、五叶期以及成熟期采集植物和土壤样品 (根际土和非根际土),测定玉米根系、地上部生物量及其磷含量,土壤碱性磷酸酶活性、pH、有效磷、丛枝菌根侵染率,并采用WinRHIZO根系扫描系统测定根长、根表面积、根体积等指标。  【结果】  三年试验结果表明,RP、BF、SA处理玉米籽粒产量、生物量与FP处理无显著差异 (P > 0.05),但磷肥偏生产力显著提高,平均增幅68.0%。RP、BF、SA处理成熟期玉米籽粒磷含量较FP处理降低7.1%~12.9%,磷累积量降低了8.8%~17.0%,其中RP和BF处理降低幅度达到显著水平,但SA处理籽粒磷含量和磷累积量与FP处理相当。与FP处理相比,RP和SA的磷肥回收利用率均有提高,其中SA处理显著提高7.2个百分点;RP、BF、SA处理均有促进春玉米苗期根系生长的趋势,其中SA处理根长、根表面积以及细根长 (直径小于0.50 mm) 分别提高13.9%~37.9%、8.6%~46.1%、12.2%~43.0%。此外,与FP处理相比,RP和SA处理提高了玉米苗期根系丛枝菌根侵染率,增幅在16.2%~21.7%;SA处理非根际土碱性酶活性有增加趋势,五叶期差异达显著水平,BF处理非根际土有效磷含量提高了18.8%~56.3%。  【结论】  在西北旱地春玉米种植区,磷肥施用量由现在的P2O5 120 kg/hm2减少至70~75 kg/hm2仍可保证玉米稳产。在此基础上,用生理酸性肥料硫酸铵代替尿素可促进玉米根系生长以及丛枝菌根侵染,促进玉米对磷素的吸收利用。  相似文献   
98.
【目的】 筛选不同温度下烟草花叶病毒(Tobacco mosaic virus,TMV)侵染后枯斑三生烟(Nicotiana tabacum var. Samsun NN)差异表达的长链非编码RNA(long non-coding RNA,lncRNA),研究lncRNA在枯斑三生烟抗性反应中的作用。【方法】 N基因的温度敏感性使枯斑三生烟在25℃时具备对TMV的抗性、在31℃抗性丧失,在这两个温度条件下对枯斑三生烟接种TMV和磷酸盐缓冲盐水(phosphate buffered saline,PBS),48 h后提取系统叶总RNA,构建链特异性文库后进行深度测序。对测序结果进行过滤后利用HTSeq将有效数据与近缘品种TN90(N. tabacum var. TN90)基因组比对,筛选得到lncRNA后利用FPKM法估计lncRNA的表达水平。通过edgeR筛选差异表达lncRNA(differentially expressed lncRNA,DElncRNA),并利用qRT-PCR技术对这一结果进行验证。通过共定位及共表达分析预测DElncRNA的靶基因,通过参考基因组注释、GO和KEGG富集分析研究靶基因的功能。【结果】 4个处理共12个样本经lncRNA-seq各测得约8 000万条clean reads,共获得4 737条已知lncRNA、40 169条新lncRNA。其中64个lncRNA在不同温度条件下TMV侵染后存在差异表达,qRT-PCR测定结果显示这些lncRNA的测序正确率在80%左右,表明本研究所得测序数据具备较高的可信度。对DElncRNA进行靶基因预测,发现一些基因同时被25℃下调和31℃上调的DElncRNA靶向。靶基因注释功能丰富,主要参与植物抗病、激素和代谢等生理过程。部分可能与激素通路相关的lncRNA,在25℃下TMV侵染时呈现下调趋势,而在31℃下TMV侵染则呈现上调趋势。GO富集分析显示靶基因主要参与构成膜、囊泡等组分,具备钙、钾离子通道抑制剂活性等分子功能,使相应离子得以转运引发随后的反应,同时也参与发病、抗原加工和呈现、细胞分裂素代谢等生理过程。KEGG分析发现靶基因显著富集在植物激素信号转导通路,25℃下调和31℃上调的DElncRNA靶基因同时富集在激素信号传导、ABC运输蛋白、苯丙烷类生物合成等通路。【结论】 不同温度(25℃和31℃)条件下TMV侵染枯斑三生烟后,长链非编码RNA差异表达,DElncRNA通过作用于激素信号传导、物质转运等过程参与寄主系统获得性抗性反应。研究结果可为揭示植物系统获得性抗性中lncRNA的调控功能以及新型抗病毒技术开发提供依据。  相似文献   
99.
100.
A new functional porous carbons (PC-WF) is prepared by activation-pyrolysis method use waste cotton fabrics (WF) as an abundant, cheap and available precursor for removal of Brilliant Crocein (BC-GR) and Cationic Red 2GL (CR-GL) from aqueous phase. The PC-WF was characterized by BET, FTIR, SEM, and XRD techniques, the surface area, total pore volume, average pore diameter was found as 1463.5 m2 g-1, 0.783 cm3 g-1 and 2.14 nm, respectively. The influences on BC-GR and CR-GL adsorption of various experimental factors such as initial concentration and temperature were investigated. Adsorption kinetics was found to be best represented by the pseudo-second order model. The adsorption capacity was 319.8 mg g-1 for BC-GR and 842.5 mg g-1 for CR-GL at 30 °C, respectively. The results indicate that for waste cotton fabrics in particular, the practical application of this process to the production of porous carbon would be possible.  相似文献   
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