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稀土对阿尔巴斯白绒山羊瘤胃发酵,消化代谢及其生产性能的影响 总被引:5,自引:0,他引:5
实验1用4只安装永久性瘤胃漏管的阿尔巴斯白绒山羊(母),进行瘤胃消化代谢试验;实验2用6只成年母绒山羊进行消化代谢试验;试验3选用同品种成年妊娠母羊进行放牧补饲实验。实验1得出,稀土的使用可促进瘤胃内NH3-N的利用;细菌数比对照组提高35.67%;丙酸产量提高28.63%。实验2得出,稀土可提高绒山羊日粮DM、OM、GE及ADF消化率,分别为11.58%、12.27%、16.69%和26.29%;每日氮存留率比对照组提高180.1%。实验3得出,稀土组产绒量比对照组提高8.90%。 相似文献
14.
β-葡聚糖对肉仔鸡生产性能及免疫功能的影响 总被引:6,自引:0,他引:6
选用1日龄AA肉仔鸡480只(公雏),随机分为5个处理组。每个处理6个重复,对照组饲喂基础日粮,抗生素组饲喂基础日粮 林可霉素,三个β-葡聚糖组,在基础日粮中分别添加25、50、100mg/kgβ-葡聚糖,以研究添加β-葡聚糖对肉鸡生产性能、免疫器官、细胞和体液免疫、血清中溶菌酶含量以及盲肠内乳酸杆菌的影响。结果显示:肉仔鸡日粮中添加50mg/kgβ-葡聚糖,显著提高0~49日龄日增重和饲料转化效率(P<0.05);显著提高21日龄脾脏的相对重量(P<0.05);添加β-葡聚糖有提高非特异性免疫力的趋势。 相似文献
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DingXiaodong YanLibo 《东北农业大学学报(英文版)》1996,3(1):55-62
Ribes plants,like most of other fruit trees ,are characterized by their large number but small size of somatic chromosomes.In the light of these spccial characteristies,we have examined several factorial combinations of material,pretreatment,slide preparing method and stain to identify the most promising techique for studying the chromosomes and further analyzing the karyotypes and chromosome banding of Ribes plants.The resuts indicated that the combination of root tip with pretreatment of 2m molL^-1 8-hydroxyquinoline plus 0.05% colchicine or 0.3% balm for 3-5 hours at 14℃,pre-hypotonic treatment of 0.07mol L^-1 KCl for 30min,fixation in Carnoy‘s fluid,hydrolysis in 5% cellulase and 5% pectinase mixture for 4-5 hours at 25℃,post-hypotoic treatment in distilled water for half an hour and staining in Giemsa Could make the chromosome preparation superior to other treatment combinations. 相似文献
17.
赤羽病微量病毒中和试验诊断方法的研究 总被引:6,自引:1,他引:5
应用引自日本的赤羽病病毒,制备了微量病毒中和试验抗原和高免阳性血清,建立了赤羽病微量病毒中和试验诊断方法,应用此方法对广东省724头牛血清进行了检查,阳性率为35.3%,对上海665头牛血清作了检查,阳性率为67.7%,说明这些地区曾流行过赤羽病,同时对澳大利亚当地牛108头进行检查,阳性率为55.5%,与外报道一致。对澳大利亚、加拿大,美国进口牛173头的检查结果,全部阴性,对澳大利亚进口羊992头的检查结果,发现阳性1头,已得到澳方认可。 相似文献
18.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
19.
蝴蝶花(IrisjaponicaThunb.)是鸢尾科鸢尾属的多年生常绿宿根草本植物,地下有横走的根状茎;耐荫,是良好的耐荫地被植物。试验于2003年10月至2004年5月进行。选择健康的蝴蝶花植株,取地下的根状茎,选带节的茎段作为外植体,用洗洁精溶液浸泡4~5min,采用75%乙醇60s结合0.1%HgCl212min进行灭菌。根状茎在培养基MS 6BA1.0mg·L-1 KT2.0mg·L-1 NAA0.1mg·L-1,25℃下黑暗培养,腋芽出芽率达65.4%。黑暗比光照更有利于腋芽的萌发,可能是由于蝴蝶花是阴性植物;在自然条件下其根状茎的生长和顶芽的萌发处于暗环境,离体条件下黑暗培养更符合其… 相似文献
20.
Tsutomu MATSUMOTO Yuichiro NARA Hiromitsu FURUYA Harumi TAKAHASHI Kiichi TAIRAKO Hideki YAMAMOTO 《Journal of General Plant Pathology》2002,68(4):382-384
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited.
Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these
results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established.
Received 10 June 2002/ Accepted in revised form 30 October 2002 相似文献